The Experts below are selected from a list of 8382 Experts worldwide ranked by ideXlab platform
Maria Bryszewska - One of the best experts on this subject based on the ideXlab platform.
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Dendrimer mediated targeting of siRNA against polo‐like kinase for the treatment of triple negative breast cancer
Journal of Biomedical Materials Research Part A, 2019Co-Authors: Anjali Jain, Maria Bryszewska, Shaheen Mahira, Jean Pierre Majoral, Wahid Khan, Maksim IonovAbstract:Irresponsiveness of triple negative breast cancer (TNBC) toward conventional therapies has drawn attention toward siRNA therapeutics. In gene delivery, dendrimers are gaining significant attention due to their characteristic features and polo-like kinase (PLK1) is reported as a potential target for TNBC. In this work, phosphorus and Polyamidoamine dendrimer (generation 3 and 4 of each type) are explored to address delivery challenges of PLK1 siRNA (siPLK1). Dendriplexes were formed and complexation was found at 3:1 N/P ratio for all dendrimers by gel electrophoresis. Complexation was also supported by zeta potential, circular dichroism and intercalation assay. Dendriplexes were found to be stable in presence of ribonuclease and serum. Dendriplexes resulted in enhanced cell uptake of siPLK1 compared to siPLK1 solution in MDA-MB-231 and MCF-7 cells. Dendriplexes caused increased cell arrest in sub-G1 phase compared to solution. These observations suggested phosphorus and Polyamidoamine dendrimers as potential carriers for siPLK1 delivery to treat TNBC.
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Haemolytic activity of Polyamidoamine dendrimers and the protective role of human serum albumin
Proceedings of the Royal Society A: Mathematical Physical and Engineering Sciences, 2009Co-Authors: Barbara Klajnert, Sylwia Pikala, Maria BryszewskaAbstract:We have examined the haemolytic activity of Polyamidoamine dendrimers. Haemolysis increased in a generation- and concentration-dependent manner. Moreover, the longer the incubation time, the greate...
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Use of Polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells.
Molecular biology reports, 2009Co-Authors: Antos Shakhbazau, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, S. M. Kosmacheva, M. P. Potapnev, Barbara Gabara, Maxim Ionov, Maria BryszewskaAbstract:We report the use of Polyamidoamine (PAMAM-NH(2)) dendrimers along with other non-viral vehicles for the in vitro transfection of human bone marrow mesenchymal stem cells (hMSCs) and for engineering MSCs to secrete brain-derived neurotrophic factor (BDNF). Different generations of cationic Polyamidoamine dendrimers (generations 3-6) were tested on HEK 293T cells. hMSCs were then transfected with PAMAM-NH(2) G4 dendrimers and Lipofectamine 2000, which elicited the expression of GFP reporter in around 6 and 20% of the cells, respectively. Both vehicles were then shown to elicit the expression of BDNF in MSCs from a bicistronic cassette. Non-virally induced neurotrophin expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural system injuries.
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Use of a Spectrofluorimetric Method to Monitor Changes of Human Serum Albumin Thermal Stability in the Presence of Polyamidoamine Dendrimers
Journal of Fluorescence, 2006Co-Authors: M. Jokiel, Barbara Klajnert, Maria BryszewskaAbstract:Polyamidoamine (PAMAM) dendrimers' impact on human serum albumin thermal stability was investigated. Thermal denaturation profiles were determined from changes in the intrinsic fluorescence intensity. In the presence of dendrimers the shift of a denaturation temperature toward higher values was observed. It indicates a slight increase of protein stability upon dendrimers treatment.
Xiurong Yang - One of the best experts on this subject based on the ideXlab platform.
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A sensitive impedimetric thrombin aptasensor based on Polyamidoamine dendrimer.
Talanta, 2009Co-Authors: Zhanxia Zhang, Wen Yang, Juan Wang, Cheng Yang, Fan Yang, Xiurong YangAbstract:A label-free and highly sensitive impedimetric aptasensor based on a Polyamidoamine dendrimer modified gold electrode was developed for the determination of thrombin. Amino-terminated Polyamidoamine dendrimer was firstly covalently attached to the cysteine functionalized gold electrode through glutaraldehyde coupling. Subsequently, the dendrimer was activated with glutaraldehyde, and amino-modified thrombin aptamer probe was immobilized onto the activated dendrimer monolayer film. The layer-by-layer assembly process was traced by surface plasmon resonance and electrochemical impedance spectroscopy. After electrode preparation, the detection of thrombin was investigated in the presence of the reversible [Fe(CN)6](3-/4-) redox couple using impedance technique. The results showed that the charge-transfer resistance (Rct) value had a linear relationship with the concentrations of thrombin in the range of 1-50 nM, and the detection limit (S/N=3) as low as 0.01 nM was obtained. The covalent immobilization of dendrimer on the electrode surface not only improved the immobilization capacity of probe molecules but also magnified the response signal. The aptasensor exhibited favorable regeneration ability, selectivity and stability. It also showed the detectability in biological fluid.
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A sensitive impedimetric thrombin aptasensor based on Polyamidoamine dendrimer.
Talanta, 2009Co-Authors: Zhanxia Zhang, Wen Yang, Juan Wang, Cheng Yang, Fan Yang, Xiurong YangAbstract:A label-free and highly sensitive impedimetric aptasensor based on a Polyamidoamine dendrimer modified gold electrode was developed for the determination of thrombin. Amino-terminated Polyamidoamine dendrimer was firstly covalently attached to the cysteine functionalized gold electrode through glutaraldehyde coupling. Subsequently, the dendrimer was activated with glutaraldehyde, and amino-modified thrombin aptamer probe was immobilized onto the activated dendrimer monolayer film. The layer-by-layer assembly process was traced by surface plasmon resonance and electrochemical impedance spectroscopy.
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Polyamidoamine dendrimers-assisted electrodeposition of gold-platinum bimetallic nanoflowers.
The journal of physical chemistry. B, 2006Co-Authors: Lei Qian, Xiurong YangAbstract:Novel Au−Pt bimetallic flower nanostructures fabricated on a Polyamidoamine dendrimers-modified surface by electrodeposition are reported. These Polyamidoamine dendrimers were stable, and they assisted the formation of Au−Pt bimetallic nanoflowers during the electrodeposition process. These nanoflowers were characterized by field-emitted scanning electron microscopy (FE-SEM), energy-dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction, and electrochemical methods. FE-SEM images showed that the bimetallic nanoflower included two parts: the “light” and the “pale” part. The two parts consisted of many small bimetallic nanoparticles, which was attributed to the progressive nucleation process. Moreover, the “light” part contained more bimetallic nanoparticles. The morphologies of bimetallic nanoflowers depended on the electrodeposition time and potential and the layer number of assembled dendrimers. The average size of nanoflowers increased with the increase in electrodepos...
Maksim Ionov - One of the best experts on this subject based on the ideXlab platform.
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Dendrimer mediated targeting of siRNA against polo‐like kinase for the treatment of triple negative breast cancer
Journal of Biomedical Materials Research Part A, 2019Co-Authors: Anjali Jain, Maria Bryszewska, Shaheen Mahira, Jean Pierre Majoral, Wahid Khan, Maksim IonovAbstract:Irresponsiveness of triple negative breast cancer (TNBC) toward conventional therapies has drawn attention toward siRNA therapeutics. In gene delivery, dendrimers are gaining significant attention due to their characteristic features and polo-like kinase (PLK1) is reported as a potential target for TNBC. In this work, phosphorus and Polyamidoamine dendrimer (generation 3 and 4 of each type) are explored to address delivery challenges of PLK1 siRNA (siPLK1). Dendriplexes were formed and complexation was found at 3:1 N/P ratio for all dendrimers by gel electrophoresis. Complexation was also supported by zeta potential, circular dichroism and intercalation assay. Dendriplexes were found to be stable in presence of ribonuclease and serum. Dendriplexes resulted in enhanced cell uptake of siPLK1 compared to siPLK1 solution in MDA-MB-231 and MCF-7 cells. Dendriplexes caused increased cell arrest in sub-G1 phase compared to solution. These observations suggested phosphorus and Polyamidoamine dendrimers as potential carriers for siPLK1 delivery to treat TNBC.
Zhanxia Zhang - One of the best experts on this subject based on the ideXlab platform.
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A sensitive impedimetric thrombin aptasensor based on Polyamidoamine dendrimer.
Talanta, 2009Co-Authors: Zhanxia Zhang, Wen Yang, Juan Wang, Cheng Yang, Fan Yang, Xiurong YangAbstract:A label-free and highly sensitive impedimetric aptasensor based on a Polyamidoamine dendrimer modified gold electrode was developed for the determination of thrombin. Amino-terminated Polyamidoamine dendrimer was firstly covalently attached to the cysteine functionalized gold electrode through glutaraldehyde coupling. Subsequently, the dendrimer was activated with glutaraldehyde, and amino-modified thrombin aptamer probe was immobilized onto the activated dendrimer monolayer film. The layer-by-layer assembly process was traced by surface plasmon resonance and electrochemical impedance spectroscopy.
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A sensitive impedimetric thrombin aptasensor based on Polyamidoamine dendrimer.
Talanta, 2009Co-Authors: Zhanxia Zhang, Wen Yang, Juan Wang, Cheng Yang, Fan Yang, Xiurong YangAbstract:A label-free and highly sensitive impedimetric aptasensor based on a Polyamidoamine dendrimer modified gold electrode was developed for the determination of thrombin. Amino-terminated Polyamidoamine dendrimer was firstly covalently attached to the cysteine functionalized gold electrode through glutaraldehyde coupling. Subsequently, the dendrimer was activated with glutaraldehyde, and amino-modified thrombin aptamer probe was immobilized onto the activated dendrimer monolayer film. The layer-by-layer assembly process was traced by surface plasmon resonance and electrochemical impedance spectroscopy. After electrode preparation, the detection of thrombin was investigated in the presence of the reversible [Fe(CN)6](3-/4-) redox couple using impedance technique. The results showed that the charge-transfer resistance (Rct) value had a linear relationship with the concentrations of thrombin in the range of 1-50 nM, and the detection limit (S/N=3) as low as 0.01 nM was obtained. The covalent immobilization of dendrimer on the electrode surface not only improved the immobilization capacity of probe molecules but also magnified the response signal. The aptasensor exhibited favorable regeneration ability, selectivity and stability. It also showed the detectability in biological fluid.
H A Tajmirriahi - One of the best experts on this subject based on the ideXlab platform.
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review on the targeted conjugation of anticancer drugs doxorubicin and tamoxifen with synthetic polymers for drug delivery
Journal of Biomolecular Structure & Dynamics, 2017Co-Authors: S Sanyakamdhorn, Daniel Agudelo, H A TajmirriahiAbstract:In this review, the binding and loading efficacy (LE) of anticancer drugs doxorubicin (DOX), tamoxifen (Tam) and its metabolites 4-hydroxytamoxifen (4-Hydroxytam) and endoxifen (Endox) with several synthetic polymers poly(ethylene glycol) (PEG), methoxypoly (ethylene glycol) Polyamidoamine (mPEG-PAMAM-G3), and Polyamidoamine (PAMAM-G4) dendrimers were compared in aqueous solution at pH 7.4. The results of multiple spectroscopic methods, transmission electron microscopy (TEM) and molecular modeling of conjugated drug–polymer were examined. Structural analysis showed that drug–polymer conjugation occurs mainly via H-bonding and hydrophobic contacts. The order of binding is PAMAM-G4 > mPEG-PAMAM-G3 > PEG-6000 with 4-hydroxttamoxifen forming more stable conjugate than tamoxifen and endoxifen. Doxorubicin shows stronger affinity for PAMAM-G4 than tamoxifen and its metabolites. The drug LE was 30–55%. TEM showed significant changes in the carrier morphology upon drug encapsulation. Modeling also showed that dru...
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targeted conjugation of breast anticancer drug tamoxifen and its metabolites with synthetic polymers
Colloids and Surfaces B: Biointerfaces, 2016Co-Authors: S Sanyakamdhorn, Daniel Agudelo, L Bekale, H A TajmirriahiAbstract:Conjugation of antitumor drug tamoxifen and its metabolites, 4-hydroxytamxifen and ednoxifen with synthetic polymers poly(ethylene glycol) (PEG), methoxypoly (ethylene glycol) Polyamidoamine (mPEG-PAMAM-G3) and Polyamidoamine (PAMAM-G4) dendrimers was studied in aqueous solution at pH 7.4. Multiple spectroscopic methods, transmission electron microscopy (TEM) and molecular modeling were used to characterize the drug binding process to synthetic polymers. Structural analysis showed that drug-polymer binding occurs via both H-bonding and hydrophobic contacts. The order of binding is PAMAM-G4>mPEG-PAMAM-G3>PEG-6000 with 4-hydroxttamoxifen forming more stable conjugate than tamoxifen and endoxifen. Transmission electron microscopy showed significant changes in carrier morphology with major changes in the shape of the polymer aggregate as drug encapsulation occurred. Modeling also showed that drug is located in the surface and in the internal cavities of PAMAM with the free binding energy of -3.79 for tamoxifen, -3.70 for 4-hydroxytamoxifen and -3.69kcal/mol for endoxifen, indicating of spontaneous drug-polymer interaction at room temperature.
