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P C Lau - One of the best experts on this subject based on the ideXlab platform.
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characterization of the genes encoding a receptor like histidine kinase and a cognate response regulator from a biphenyl Polychlorobiphenyl degrading bacterium rhodococcus sp strain m5
Journal of Bacteriology, 1997Co-Authors: D Labbe, J Garnon, P C LauAbstract:We report the cloning, sequence, and expression of the bpdS and bpdT genes from Rhodococcus sp. strain M5, which are believed to encode the first two-component signal transduction system in the genus Rhodococcus, which potentially regulates biphenyl/Polychlorobiphenyl metabolism in M5. BpdT has a typical responses regulator sequence (209 amino acids; 23 kDa), whereas BpdS, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kDa) that contains ATP-binding and leucine-rich repeat motifs and some conserved residues of protein kinases. Expression of bpdST, like that of the bpdC1C2BADE degradative operon, is inducible by biphenyl.
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Characterization of the Genes Encoding a Receptor-Like
1996Co-Authors: D Labbé, J Garnon, P C Lau, J. BacteriolAbstract:Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/Polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5
D Labbe - One of the best experts on this subject based on the ideXlab platform.
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characterization of the genes encoding a receptor like histidine kinase and a cognate response regulator from a biphenyl Polychlorobiphenyl degrading bacterium rhodococcus sp strain m5
Journal of Bacteriology, 1997Co-Authors: D Labbe, J Garnon, P C LauAbstract:We report the cloning, sequence, and expression of the bpdS and bpdT genes from Rhodococcus sp. strain M5, which are believed to encode the first two-component signal transduction system in the genus Rhodococcus, which potentially regulates biphenyl/Polychlorobiphenyl metabolism in M5. BpdT has a typical responses regulator sequence (209 amino acids; 23 kDa), whereas BpdS, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kDa) that contains ATP-binding and leucine-rich repeat motifs and some conserved residues of protein kinases. Expression of bpdST, like that of the bpdC1C2BADE degradative operon, is inducible by biphenyl.
J Garnon - One of the best experts on this subject based on the ideXlab platform.
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characterization of the genes encoding a receptor like histidine kinase and a cognate response regulator from a biphenyl Polychlorobiphenyl degrading bacterium rhodococcus sp strain m5
Journal of Bacteriology, 1997Co-Authors: D Labbe, J Garnon, P C LauAbstract:We report the cloning, sequence, and expression of the bpdS and bpdT genes from Rhodococcus sp. strain M5, which are believed to encode the first two-component signal transduction system in the genus Rhodococcus, which potentially regulates biphenyl/Polychlorobiphenyl metabolism in M5. BpdT has a typical responses regulator sequence (209 amino acids; 23 kDa), whereas BpdS, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kDa) that contains ATP-binding and leucine-rich repeat motifs and some conserved residues of protein kinases. Expression of bpdST, like that of the bpdC1C2BADE degradative operon, is inducible by biphenyl.
Roberto Caproni - One of the best experts on this subject based on the ideXlab platform.
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Gas Chromatography-Mass Spectrometry: Application of New Findings in Analyzing Organochlorine Contaminants in a Candidate Food Reference Material
Food Analytical Methods, 2019Co-Authors: Maurizio Masci, Teresina Nevigato, Roberto CaproniAbstract:Organochlorines are common toxic contaminants in the food chain, especially in aquatic biota, thus leading the world population to undesired exposures. The chemical analyses of such trace pollutants may represent a challenge: if not properly conducted, they could suffer from great uncertainties. In the present work, a new candidate reference material of oyster tissue was analyzed for the content of organochlorines. This was done within the H2020 project PRO-METROFOOD to which 17 European Countries participated. Our laboratory strictly applied the international guidelines on the subject. Even by following the recommended guidelines, however, it is difficult to analyze some Polychlorobiphenyls (PCBs). The chromatographic coelution of Polychlorobiphenyls with the same degree of chlorination or coelutions between Polychlorobiphenyls different for one chlorine only (isobaric interferences), for example, is to be cited. For a reliable quantification, therefore, we usefully exploited some innovative findings recently made by us in the field of mass spectrometry: they allowed to achieve an optimum accuracy in the case of isobaric interferences so ensuring that a correct food safety assessment is carried out. When Polychlorobiphenyls are analyzed, there are a number of good reasons for maintaining the use of the electron capture detection (ECD) in combination with the mass spectrometry. However, the present study highlights the limits and the critical points of the approach using ECD only, as many laboratories do.
D Labbé - One of the best experts on this subject based on the ideXlab platform.
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Characterization of the Genes Encoding a Receptor-Like
1996Co-Authors: D Labbé, J Garnon, P C Lau, J. BacteriolAbstract:Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/Polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5