The Experts below are selected from a list of 76197 Experts worldwide ranked by ideXlab platform
Jose A Pomposo - One of the best experts on this subject based on the ideXlab platform.
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ph responsive single chain Polymer Nanoparticles utilising dynamic covalent enamine bonds
Chemical Communications, 2014Co-Authors: David A Fulton, Ana Sanchezsanchez, Jose A PomposoAbstract:Structurally dynamic single-chain Polymer Nanoparticles that can reversibly undergo a coil to particle transition via formation and cleavage of intramolecular dynamic enamine cross-links are reported.
Jesse V Jokerst - One of the best experts on this subject based on the ideXlab platform.
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molecular afterglow imaging with bright biodegradable Polymer Nanoparticles
Nature Biotechnology, 2017Co-Authors: Qingqing Miao, Xu Zhen, Hongwei Duan, Jesse V Jokerst, Kanyi PuAbstract:Ultra-high signal-to-background in vivo imaging is enabled by biocompatible semiconducting Polymer Nanoparticles.
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molecular afterglow imaging with bright biodegradable Polymer Nanoparticles
Nature Biotechnology, 2017Co-Authors: Qingqing Miao, Xu Zhen, Hongwei Duan, Yan Lyu, Chen Xie, Xiaogang Liu, Jesse V JokerstAbstract:Afterglow optical agents, which emit light long after cessation of excitation, hold promise for ultrasensitive in vivo imaging because they eliminate tissue autofluorescence. However, afterglow imaging has been limited by its reliance on inorganic Nanoparticles with relatively low brightness and short-near-infrared (NIR) emission. Here we present semiconducting Polymer Nanoparticles (SPNs) <40 nm in diameter that store photon energy via chemical defects and emit long-NIR afterglow luminescence at 780 nm with a half-life of ∼6 min. In vivo, the afterglow intensity of SPNs is more than 100-fold brighter than that of inorganic afterglow agents, and the signal is detectable through the body of a live mouse. High-contrast lymph node and tumor imaging in living mice is demonstrated with a signal-to-background ratio up to 127-times higher than that obtained by NIR fluorescence imaging. Moreover, we developed an afterglow probe, activated only in the presence of biothiols, for early detection of drug-induced hepatotoxicity in living mice.
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diketopyrrolopyrrole based semiconducting Polymer Nanoparticles for in vivo photoacoustic imaging
Advanced Materials, 2015Co-Authors: Jesse V Jokerst, Adam J Shuhendler, Jianguo Mei, Guosong Hong, Alexander L Antaris, Niladri Chattopadhyay, Tadanori Kurosawa, Yan Zhou, Sanjiv S GambhirAbstract:Diketopyrrolopyrrole-based semiconducting Polymer Nanoparticles with high photostability and strong photoacoustic brightness are designed and synthesized, which results in 5.3-fold photoacoustic signal enhancement in tumor xenografts after systemic administration.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Adam J Shuhendler, Jesse V Jokerst, Jianguo Mei, Sanjiv S Gambhir, Zhenan Bao, Jianghong RaoAbstract:Photoacoustic imaging holds great promise for the visualization of physiology and pathology at the molecular level with deep tissue penetration and fine spatial resolution. To fully utilize this potential, photoacoustic molecular imaging probes have to be developed. Here, we introduce near-infrared light absorbing semiconducting Polymer Nanoparticles as a new class of contrast agents for photoacoustic molecular imaging. These Nanoparticles can produce a stronger signal than the commonly used single-walled carbon nanotubes and gold nanorods on a per mass basis, permitting whole-body lymph-node photoacoustic mapping in living mice at a low systemic injection mass. Furthermore, the semiconducting Polymer Nanoparticles possess high structural flexibility, narrow photoacoustic spectral profiles and strong resistance to photodegradation and oxidation, enabling the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time imaging of reactive oxygen species--vital chemical mediators of many diseases. These results demonstrate semiconducting Polymer Nanoparticles to be an ideal nanoplatform for developing photoacoustic molecular probes.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Kanyi Pu, Jesse V Jokerst, Adam J Shuhendler, Sanjiv S GambhirAbstract:Polymer Nanoparticles that absorb near-infrared light can be used for photoacoustic imaging with clear advantages over existing contrast agents.
Sanjiv S Gambhir - One of the best experts on this subject based on the ideXlab platform.
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diketopyrrolopyrrole based semiconducting Polymer Nanoparticles for in vivo photoacoustic imaging
Advanced Materials, 2015Co-Authors: Jesse V Jokerst, Adam J Shuhendler, Jianguo Mei, Guosong Hong, Alexander L Antaris, Niladri Chattopadhyay, Tadanori Kurosawa, Yan Zhou, Sanjiv S GambhirAbstract:Diketopyrrolopyrrole-based semiconducting Polymer Nanoparticles with high photostability and strong photoacoustic brightness are designed and synthesized, which results in 5.3-fold photoacoustic signal enhancement in tumor xenografts after systemic administration.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Adam J Shuhendler, Jesse V Jokerst, Jianguo Mei, Sanjiv S Gambhir, Zhenan Bao, Jianghong RaoAbstract:Photoacoustic imaging holds great promise for the visualization of physiology and pathology at the molecular level with deep tissue penetration and fine spatial resolution. To fully utilize this potential, photoacoustic molecular imaging probes have to be developed. Here, we introduce near-infrared light absorbing semiconducting Polymer Nanoparticles as a new class of contrast agents for photoacoustic molecular imaging. These Nanoparticles can produce a stronger signal than the commonly used single-walled carbon nanotubes and gold nanorods on a per mass basis, permitting whole-body lymph-node photoacoustic mapping in living mice at a low systemic injection mass. Furthermore, the semiconducting Polymer Nanoparticles possess high structural flexibility, narrow photoacoustic spectral profiles and strong resistance to photodegradation and oxidation, enabling the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time imaging of reactive oxygen species--vital chemical mediators of many diseases. These results demonstrate semiconducting Polymer Nanoparticles to be an ideal nanoplatform for developing photoacoustic molecular probes.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Kanyi Pu, Jesse V Jokerst, Adam J Shuhendler, Sanjiv S GambhirAbstract:Polymer Nanoparticles that absorb near-infrared light can be used for photoacoustic imaging with clear advantages over existing contrast agents.
Kenneth J Shea - One of the best experts on this subject based on the ideXlab platform.
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Preparation of abiotic Polymer Nanoparticles for sequestration and neutralization of a target peptide toxin
Nature Protocols, 2015Co-Authors: Keiichi Yoshimatsu, Yu Hoshino, Hiroyuki Koide, Kenneth J SheaAbstract:Synthetic Polymer Nanoparticles (NPs) with intrinsic affinity for target biomacromolecules hold great promise in the development of novel tools for biological and biomedical research. We recently reported the design and synthesis of abiotic, synthetic Polymer NPs with high intrinsic affinity for a peptide toxin melittin. The NP was selected by screening a small library of NPs (∼100 nm) composed of various ratios of monomers that contain functional groups complementary to the peptide melittin. The selected Polymer NP, a co-Polymer of acrylic acid (AAc), N - tert -butylacrylamide (TBAm), N -isopropylacrylamide (NIPAm) and N , N ′-methylenebisacrylamide (BIS), effectively captures and neutralizes the toxicity of the peptide through a combination of electrostatic and hydrophobic interactions. This protocol describes a step-by-step procedure for the preparation and evaluation of synthetic Polymer NPs for sequestration and neutralization of the target peptide toxin. The Polymer NPs can be synthesized in a one-step Polymerization reaction using commercially available reagents. The Polymerization reaction for the synthesis of Polymer NPs takes several hours, and the total protocol including subsequent purification and characterization by dynamic light scattering, NMR and toxicity neutralization assays takes 1–2 weeks in total. Through screening libraries of Polymer Nanoparticles (NPs) containing various comonomers, it is possible to develop NPs with high affinity for target biomacromolecules. This protocol describes the preparation and characterization of melittin-binding NPs.
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recognition neutralization and clearance of target peptides in the bloodstream of living mice by molecularly imprinted Polymer Nanoparticles a plastic antibody
Journal of the American Chemical Society, 2010Co-Authors: Yu Hoshino, Hiroyuki Koide, Takeo Urakami, Hiroaki Kanazawa, Takashi Kodama, Naoto Oku, Kenneth J SheaAbstract:We report that simple, synthetic organic Polymer Nanoparticles (NPs) can capture and clear a target peptide toxin in the bloodstream of living mice. The protein-sized Polymer Nanoparticles, with a binding affinity and selectivity comparable to those of natural antibodies, were prepared by combining a functional monomer optimization strategy with molecular-imprinting nanoparticle synthesis. As a result of binding and removal of melittin by NPs in vivo, the mortality and peripheral toxic symptoms due to melittin were significantly diminished. In vivo imaging of the Polymer Nanoparticles (or “plastic antibodies”) established that the NPs accelerate clearance of the peptide from blood and accumulate in the liver. Coupled with their biocompatibility and nontoxic characteristics, plastic antibodies offer the potential for neutralizing a wide range of biomacromolecules in vivo.
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synthetic Polymer Nanoparticles with antibody like affinity for a hydrophilic peptide
ACS Nano, 2010Co-Authors: Zhiyang Zeng, Yu Hoshino, Andy Rodriguez, Hoseong Yoo, Kenneth J SheaAbstract:Synthetic Polymer Nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion Polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the Polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting Polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during Polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide−NP binding affinities (ca. 90−900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the Nanoparticles. This method can be used for the preparation of nanosized synthetic Polymers with antibody-like affinity for hydrophilic peptides and proteins (“plastic antibodies”).
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peptide imprinted Polymer Nanoparticles a plastic antibody
Journal of the American Chemical Society, 2008Co-Authors: Yu Hoshino, Takashi Kodama, Yoshio Okahata, Kenneth J SheaAbstract:A novel method for preparation of biomacromolecular imprinted Nanoparticles is described. Combinations of functional monomers were Polymerized in the presence of the imprinting peptide melittin in aqueous solution at room temperature to produce a small library of Polymer Nanoparticles. The template peptide and unreacted monomers are subsequently removed by dialysis. Nanoparticles (NPs) from the library were evaluated for their binding to melittin by 27 MHz QCM analysis. NPs prepared with optimized functional monomer combinations bind strongly to the target molecule. Nanoparticles that were Polymerized in the absence of template peptide were found to have little affinity to the peptide. Binding affinity and the size of imprinted particles are comparable to those of natural antibodies. They interact specifically with the target peptide and show little affinity for other proteins. These NPs are of interest as inert and stable substitutes for antibodies. Extension of this approach to other targets of biological importance and the applications of these materials are currently being evaluated.
Adam J Shuhendler - One of the best experts on this subject based on the ideXlab platform.
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diketopyrrolopyrrole based semiconducting Polymer Nanoparticles for in vivo photoacoustic imaging
Advanced Materials, 2015Co-Authors: Jesse V Jokerst, Adam J Shuhendler, Jianguo Mei, Guosong Hong, Alexander L Antaris, Niladri Chattopadhyay, Tadanori Kurosawa, Yan Zhou, Sanjiv S GambhirAbstract:Diketopyrrolopyrrole-based semiconducting Polymer Nanoparticles with high photostability and strong photoacoustic brightness are designed and synthesized, which results in 5.3-fold photoacoustic signal enhancement in tumor xenografts after systemic administration.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Adam J Shuhendler, Jesse V Jokerst, Jianguo Mei, Sanjiv S Gambhir, Zhenan Bao, Jianghong RaoAbstract:Photoacoustic imaging holds great promise for the visualization of physiology and pathology at the molecular level with deep tissue penetration and fine spatial resolution. To fully utilize this potential, photoacoustic molecular imaging probes have to be developed. Here, we introduce near-infrared light absorbing semiconducting Polymer Nanoparticles as a new class of contrast agents for photoacoustic molecular imaging. These Nanoparticles can produce a stronger signal than the commonly used single-walled carbon nanotubes and gold nanorods on a per mass basis, permitting whole-body lymph-node photoacoustic mapping in living mice at a low systemic injection mass. Furthermore, the semiconducting Polymer Nanoparticles possess high structural flexibility, narrow photoacoustic spectral profiles and strong resistance to photodegradation and oxidation, enabling the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time imaging of reactive oxygen species--vital chemical mediators of many diseases. These results demonstrate semiconducting Polymer Nanoparticles to be an ideal nanoplatform for developing photoacoustic molecular probes.
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semiconducting Polymer Nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Kanyi Pu, Jesse V Jokerst, Adam J Shuhendler, Sanjiv S GambhirAbstract:Polymer Nanoparticles that absorb near-infrared light can be used for photoacoustic imaging with clear advantages over existing contrast agents.