The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Zhijun Sun - One of the best experts on this subject based on the ideXlab platform.
-
erythrocyte membrane coated upconversion nanoparticles with minimal protein adsorption for enhanced Tumor Imaging
ACS Applied Materials & Interfaces, 2017Co-Authors: Lang Rao, Qianfang Meng, Bo Cai, Qin Qin Huang, Zhijun SunAbstract:Upconversion nanoparticles (UCNPs) with superior optical and chemical features have been broadly employed for in vivo cancer Imaging. Generally, UCNPs are surface modified with ligands for cancer active targeting. However, nanoparticles in biological fluids are known to form a long-lived “protein corona”, which covers the targeting ligands on nanoparticle surface and dramatically reduces the nanoparticle targeting capabilities. Here, for the first time, we demonstrated that by coating UCNPs with red blood cell (RBC) membranes, the resulting cell membrane-capped nanoparticles (RBC-UCNPs) adsorbed virtually no proteins when exposed to human plasma. We further observed in various scenarios that the cancer targeting ability of folic acid (FA)-functionalized nanoparticles (FA-RBC-UCNPs) was rescued by the cell membrane coating. Next, the FA-RBC-UCNPs were successfully utilized for enhanced in vivo Tumor Imaging. Finally, blood parameters and histology analysis suggested that no significant systematic toxicity ...
Xiaoyuan Chen - One of the best experts on this subject based on the ideXlab platform.
-
Near-Infrared Quantum Dots as Optical Probes for Tumor Imaging
Current topics in medicinal chemistry, 2010Co-Authors: Jinhao Gao, Xiaoyuan Chen, Zhen ChengAbstract:Molecular Imaging plays a key role in personalized medicine, which is the goal and future of patient management. Among the various molecular Imaging modalities, optical Imaging may be the fastest growing area for bioanalysis, and the major reason is the research on fluorescence semiconductor quantum dots (QDs) and dyes have evolved over the past two decades. The great efforts on the synthesis of QDs with fluorescence emission from UV to near-infrared (NIR) regions speed up the studies of QDs as optical probes for in vitro and in vivo molecular Imaging. For in vivo applications, the fluorescent emission wavelength ideally should be in a region of the spectrum where blood and tissue absorb minimally and tissue penetration reach maximally, which is NIR region (typically 700-1000 nm). The goal of this review is to provide readers the basics of NIR-emitting QDs, the bioconjugate chemistry of QDs, and their applications for diagnostic Tumor Imaging. We will also discuss the benefits, challenges, limitations, perspective, and the future scope of NIR-emitting QDs for Tumor Imaging applications.
-
^64Cu-Labeled PEGylated Polyethylenimine for Cell Trafficking and Tumor Imaging
Molecular Imaging and Biology, 2009Co-Authors: Zi-bo Li, Kai Chen, Zhanhong Wu, Hui Wang, Xiaoyuan ChenAbstract:Purpose In this study, we exploited the potential of ^64Cu-labeled polyethylenimine (PEI) for cell trafficking and Tumor Imaging as compared to copper-64-pyruvaldehyde-bis( N ^4-methylthiosemicarbazone) (^64Cu-PTSM). Procedures U87MG cells were labeled with both ^64Cu-PEI and ^64Cu-PTSM, and their in vivo distributions in mice were tracked by positron emission tomography (PET). The Tumor Imaging ability of ^64Cu-PTSM and ^64Cu-PEI was investigated in U87MG human glioblastoma xenograft model. ^64Cu-PEI-polyethylene glycol (PEG) was also synthesized, and the cell uptake, efflux, cytotoxicity, and the biodistribution were carried out and compared with ^64Cu-PEI. Results Both ^64Cu-PEI and ^64Cu-PEI-PEG were obtained in high labeling yield without the need of macrocyclic chelating agents. ^64Cu-PEI showed lower cell labeling efficiency than ^64Cu-PTSM. Small-animal PET images of living mice indicate that tail-vein-injected U87MG cells labeled with ^64Cu-PTSM or ^64Cu-PEI traffic to the lungs and liver. In a subcutaneous U87MG xenograft model, ^64Cu-PEI had higher Tumor uptake (18.7 ± 2.2 %ID/g at 24 h) than ^64Cu-PTSM (12.4 ± 1.7 %ID/g at 24 h). In comparison with ^64Cu-PEI, ^64Cu-PEI-PEG had decreased toxicity and increased cell uptake in cell culture, as well as higher Tumor uptake and better Tumor-to-background contrast in U87MG xenograft model. Conclusion ^64Cu-labeled polyethylenimine can be used for both cell trafficking and Tumor Imaging. PEGylation reduces the toxicity of ^64Cu-PEI and improves the Tumor Imaging ability.
-
64Cu-labeled PEGylated polyethylenimine for cell trafficking and Tumor Imaging.
Molecular imaging and biology, 2009Co-Authors: Kai Chen, Hui Wang, Gang Niu, Xiaoyuan ChenAbstract:Purpose In this study, we exploited the potential of 64Cu-labeled polyethylenimine (PEI) for cell trafficking and Tumor Imaging as compared to copper-64-pyruvaldehyde-bis(N4-methylthiosemicarbazone) (64Cu-PTSM).
-
RESEARCH ARTICLE 64 Cu-Labeled PEGylated Polyethylenimine for Cell Trafficking and Tumor Imaging
2009Co-Authors: Kai Chen, Hui Wang, Gang Niu, Xiaoyuan ChenAbstract:Purpose: In this study, we exploited the potential of 64 Cu-labeled polyethylenimine (PEI) for cell trafficking and Tumor Imaging as compared to copper-64-pyruvaldehyde-bis(N 4 -methylthiosemicarbazone) ( 64 Cu-PTSM). Procedures: U87MG cells were labeled with both 64 Cu-PEI and 64 Cu-PTSM, and their in vivo distributions in mice were tracked by positron emission tomography (PET). The Tumor Imaging ability of 64 Cu-PTSM and 64 Cu-PEI was investigated in U87MG human glioblastoma xenograft model. 64 Cu-PEI-polyethylene glycol (PEG) was also synthesized, and the cell uptake, efflux, cytotoxicity, and the biodistribution were carried out and compared with 64 Cu-PEI. Results: Both 64 Cu-PEI and 64 Cu-PEI-PEG were obtained in high labeling yield without the need of macrocyclic chelating agents. 64 Cu-PEI showed lower cell labeling efficiency than 64 CuPTSM. Small-animal PET images of living mice indicate that tail-vein-injected U87MG cells labeled with 64 Cu-PTSM or 64 Cu-PEI traffic to the lungs and liver. In a subcutaneous U87MG xenograft model, 64 Cu-PEI had higher Tumor uptake (18.7±2.2 %ID/g at 24 h) than 64 Cu-PTSM (12.4±1.7 %ID/g at 24 h). In comparison with 64 Cu-PEI, 64 Cu-PEI-PEG had decreased toxicity and increased cell uptake in cell culture, as well as higher Tumor uptake and better Tumor-tobackground contrast in U87MG xenograft model. Conclusion: 64 Cu-labeled polyethylenimine can be used for both cell trafficking and Tumor Imaging. PEGylation reduces the toxicity of 64 Cu-PEI and improves the Tumor Imaging ability.
-
64Cu-Labeled polyethyleneimine (PEI) for cell trafficking and Tumor Imaging
The Journal of Nuclear Medicine, 2008Co-Authors: Kai Chen, Hui Wang, Gang Niu, Xiaoyuan ChenAbstract:562 Objectives: Direct visualization of cell migration patterns in vivo will greatly assist the cellular therapy regime as it supplies definitive evidence of successful targeting and allows quantification of the degree of migration to a particular site. Suitably radiolabeled cells have been proven to be a simple and sensitive technique for cell trafficking. In this study, we developed 64Cu-polyethyleneimine (64Cu-PEI) to radiolabel cells ex vivo for in vivo positron-emission tomography (PET) Imaging. Its Tumor Imaging ability was also investigated by PET. Methods: U87MG cells were labeled with both 64Cu-PEI and 64Cu-PTSM and their in vivo distributions were tracked with PET in living mice. The Tumor Imaging ability of 64Cu-PTSM and 64Cu-PEI was investigated in U87MG human glioblastoma xenograft model. PEI-PEG was also synthesized and cell uptake, cell efflux, MTT assay, and bio-distribution were carried out to evaluate these agents in vitro and in vivo. Results: Both 64Cu-PEI and 64Cu-PEI-PEG were obtained in high labeling yield. 64Cu-PEI showed comparable cell tracking ability with 64Cu-PTSM. 64Cu-PEI had higher Tumor uptake (18.7 ± 2.2 %ID/g at 24 h) and better Imaging quality than 64Cu-PTSM (12.4 ± 1.7 %ID/g at 24 h). 64Cu-PEI-PEG reduced the toxicity of 64Cu-PEI and maintained the Tumor Imaging ability. Conclusions: 64Cu-labeled polyethylenimine can be used for both cell labeling and Tumor Imaging. As one of the most popularly employed cationic gene carriers, suitably labeled PEI may also be potentially useful for tracking the delivery of DNA and RNA in vivo. Research Support: This work was supported by National Cancer Institute (NCI) (R01 CA119053, R21 CA121842, R21 CA102123, P50 CA114747, U54 CA119367, and R24 CA93862), and Department of Defense (DOD) (W81XWH-07-1-0374, W81XWH-04-1-0697, W81XWH-06-1-0665, W81XWH-06-1-0042, and DAMD17-03-1-0143).
Shang-ren Pan - One of the best experts on this subject based on the ideXlab platform.
-
[99mTc]polyamine analogs as potential Tumor Imaging agent
Drug Development Research, 2008Co-Authors: Weixing Wan, Min Yang, Shang-ren PanAbstract:Polyamines are essential for the growth and survival of all cells with biosynthesis and transportation of polyamines being very active in Tumors. With the aim of developing a new Tumor Imaging agent, three artificial synthetic polyamine analogs, diethylenetriamine (DETA), triethylenetetramine (TETA), and tetraethylenepentamine (TEPA), were labeled with 99mTc. The 99mTc-labeled complexes were characterized by TLC and studied for stability. 99mTc-DETA, 99mTc-TETA, and 99mTc-TEPA were formed in high yields and found to be stable. 99mTc-DETA, 99mTc-TETA, and 99mTc-TEPA revealed high Tumor uptakes of 0.65, 0.58, and 0.59% ID/g at 4 h pi, respectively, in HepA Tumor-bearing mice. The ratios of the Tumor to the contralataral muscle (T/CM) were 13.68, 5.99, and 9.81, respectively. SPECT images revealed clear visualization of Tumors. Excretion routes were mainly through urine and feces. The Tumor-to-muscle count density ratios of 99mTc-DETA, 99mTc-TETA, and 99mTc-TEPA were 2.47, 2.45, and 2.91, respectively. [99mTc]-labeled polyamine analogs are promising agents for Tumor Imaging and warrant further evaluation. Drug Dev Res 69:520–525, 2008. © 2008 Wiley-Liss, Inc.
Jimmy D Bell - One of the best experts on this subject based on the ideXlab platform.
-
a low molecular weight folate receptor targeted contrast agent for magnetic resonance Tumor Imaging
Molecular Imaging and Biology, 2011Co-Authors: Tammy L Kalber, Nazila Kamaly, John A Pugh, Josephine Bunch, Cameron W Mcleod, Michael R Jorgensen, Andrew D Miller, Jimmy D BellAbstract:Purpose This study aims to develop a low molecular weight folate receptor (FR) contrast agent for MR Tumor Imaging.
-
A Low Molecular Weight Folate Receptor Targeted Contrast Agent for Magnetic Resonance Tumor Imaging
Molecular Imaging and Biology, 2011Co-Authors: Tammy L Kalber, Nazila Kamaly, John A Pugh, Josephine Bunch, Cameron W Mcleod, Michael R Jorgensen, Andrew D Miller, Jimmy D BellAbstract:Purpose This study aims to develop a low molecular weight folate receptor (FR) contrast agent for MR Tumor Imaging. Procedures Gadolinium-tetraazacyclododecane tetraacetic acid (Gd.DOTA) was conjugated to folic acid to create Gd.DOTA.Folate. The efficacy of Gd.DOTA.Folate to bind FR was evaluated in vitro by inductively coupled mass spectrometry (ICP-MS) and in vivo by magnetic resonance Imaging (MRI) Tumor enhancement over 14 h, utilizing an overexpressing α-FR cell line (IGROV-1), compared to an α-FR-negative cell line (OVCAR-3). Gd.DOTA.Folate localization ex vivo was verified by laser ablation ICP-MS. Results ICP-MS confirmed Gd.DOTA.Folate uptake by IGROV-1 cells and competitive binding with free folic acid inhibited binding. IGROV-1 Tumors showed an increase in R _1 at 2 h, which increased significantly over 14 h post-Gd.DOTA.Folate with clear enhancement on MR images. This was not observed in controls. Conclusion These data support the use of FR-targeted small molecular weight MRI contrast agents for Tumor Imaging in vivo .
Lang Rao - One of the best experts on this subject based on the ideXlab platform.
-
erythrocyte membrane coated upconversion nanoparticles with minimal protein adsorption for enhanced Tumor Imaging
ACS Applied Materials & Interfaces, 2017Co-Authors: Lang Rao, Qianfang Meng, Bo Cai, Qin Qin Huang, Zhijun SunAbstract:Upconversion nanoparticles (UCNPs) with superior optical and chemical features have been broadly employed for in vivo cancer Imaging. Generally, UCNPs are surface modified with ligands for cancer active targeting. However, nanoparticles in biological fluids are known to form a long-lived “protein corona”, which covers the targeting ligands on nanoparticle surface and dramatically reduces the nanoparticle targeting capabilities. Here, for the first time, we demonstrated that by coating UCNPs with red blood cell (RBC) membranes, the resulting cell membrane-capped nanoparticles (RBC-UCNPs) adsorbed virtually no proteins when exposed to human plasma. We further observed in various scenarios that the cancer targeting ability of folic acid (FA)-functionalized nanoparticles (FA-RBC-UCNPs) was rescued by the cell membrane coating. Next, the FA-RBC-UCNPs were successfully utilized for enhanced in vivo Tumor Imaging. Finally, blood parameters and histology analysis suggested that no significant systematic toxicity ...