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Alex Sparreboom - One of the best experts on this subject based on the ideXlab platform.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ping He, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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Simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography/tandem mass spectrometry
Analytical biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid-liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50 x 2.1mm internal diameter) packed with a 3.5-microm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (approximately 0.4 ng/ml) and 1.00 microg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
Abu T.m. Serajuddin - One of the best experts on this subject based on the ideXlab platform.
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Study of Phase Behavior of Poly(ethylene glycol)–Polysorbate 80 and Poly(ethylene glycol)–Polysorbate 80–Water Mixtures
Journal of Pharmaceutical Sciences, 2000Co-Authors: Ravindra W. Tejwani, Hemant Joshi, Sailesh A. Varia, Abu T.m. SerajuddinAbstract:Abstract Mixtures of poly(ethylene glycols) (PEGs) with Polysorbate 80 are often used to dissolve poorly water‐soluble drugs in dosage forms, where Polysorbate 80 helps either in enhancing dispersion or in inhibiting precipitation of drugs once the solution is mixed with water. Binary phase diagrams of Polysorbate 80 with several low molecular weight PEGs and a ternary phase diagram of Polysorbate 80 with PEG 400 and water are presented. Two phases were observed in the binary mixtures when the concentration of PEG 200, PEG 300, PEG 400, or PEG 600 was >55%(w/w). The miscibility of the binary mixtures increases with an increase in temperature; the upper consolute temperatures of PEG 200–Polysorbate 80, PEG 300–Polysorbate 80, PEG 400–Polysorbate 80, and PEG 600–Polysorbate 80 mixtures were 100, 85, 75, and 40 °C, respectively. The upper consolute temperature of PEG 1000–Polysorbate 80 could not be determined because the melting temperature of the mixtures is ∼40 °C and the consolute temperature appeared to be less than this temperature. The decrease in upper consolute temperature with an increase in PEG molecular weight indicated a greater miscibility of the two components. In the ternary system, phase separation of Polysorbate 80 was observed when the concentration of PEG 400 was >50–60 % (w/w), possibly because of the high exclusion volume of PEG 400. © 2000 Wiley‐Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 89:946–950, 2000
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Structural properties of polyethylene glycol—Polysorbate 80 mixture, a solid dispersion vehicle
Journal of Pharmaceutical Sciences, 1992Co-Authors: Kenneth R. Morris, Gregory T. Knipp, Abu T.m. SerajuddinAbstract:The structural properties of the mixtures of Polysorbate 80 with various polyethylene glycols (PEG), viz., PEG 1000, PEG 1450, PEG 3350, and PEG 8000, have been investigated by powder X‐ray diffraction (XRD) and differential scanning calorimetric studies. These mixtures may be used as solid dispersion vehicles to insure complete dissolution of poorly water‐soluble drugs. Although Polysorbate 80 is a liquid at room temperature, the PEG‐Polysorbate 80 mixtures with up to 75% (w/w) Polysorbate 80 were solid. The XRD studies revealed that the crystal structures (d‐spacings) of the PEGs (Mr 1000, 1450, 3350, and 8000) did not change with increasing amounts of Polysorbate 80 in the mixture. The intensities of the XRD peaks, however, varied approximately in proportion to the concentration of PEG present. Similarly, the differential scanning calorimetric studies showed that the melting behavior of a PEG–Polysorbate 80 mixture was similar to that of the PEG used. The lowering of the mp of a particular PEG due to the presence of 50% (w/w) Polysorbate 80 in the mixture was
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Structural properties of polyethylene glycol—Polysorbate 80 mixture, a solid dispersion vehicle
Journal of Pharmaceutical Sciences, 1992Co-Authors: Kenneth R. Morris, Gregory T. Knipp, Abu T.m. SerajuddinAbstract:The structural properties of the mixtures of Polysorbate 80 with various polyethylene glycols (PEG), viz., PEG 1000, PEG 1450, PEG 3350, and PEG 8000, have been investigated by powder X‐ray diffraction (XRD) and differential scanning calorimetric studies. These mixtures may be used as solid dispersion vehicles to insure complete dissolution of poorly water‐soluble drugs. Although Polysorbate 80 is a liquid at room temperature, the PEG‐Polysorbate 80 mixtures with up to 75% (w/w) Polysorbate 80 were solid. The XRD studies revealed that the crystal structures (d‐spacings) of the PEGs (Mr 1000, 1450, 3350, and 8000) did not change with increasing amounts of Polysorbate 80 in the mixture. The intensities of the XRD peaks, however, varied approximately in proportion to the concentration of PEG present. Similarly, the differential scanning calorimetric studies showed that the melting behavior of a PEG–Polysorbate 80 mixture was similar to that of the PEG used. The lowering of the mp of a particular PEG due to the presence of 50% (w/w) Polysorbate 80 in the mixture was
Sharyn D Baker - One of the best experts on this subject based on the ideXlab platform.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ping He, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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Simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography/tandem mass spectrometry
Analytical biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid-liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50 x 2.1mm internal diameter) packed with a 3.5-microm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (approximately 0.4 ng/ml) and 1.00 microg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
Yasuo Oyama - One of the best experts on this subject based on the ideXlab platform.
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zinc at clinically relevant concentrations potentiates the cytotoxicity of Polysorbate 80 a non ionic surfactant
Toxicology in Vitro, 2010Co-Authors: Tomohiro M Oyama, Yoshiro Okano, Keisuke Oyama, Toshihisa B Oyama, Shiro Ishida, Yasuo OyamaAbstract:Abstract Polysorbate 80, a non-ionic surfactant, is used in the formula of water-insoluble anticancer agents for intravenous application. In our recent studies, this surfactant decreased cellular thiol content and the chemicals decreasing cellular thiol content increased intracellular Zn 2+ concentration. In this study using rat thymocytes, the effect of Polysorbate 80 on FluoZin-3 fluorescence, an indicator for intracellular Zn 2+ , and the influence of ZnCl 2 on cytotoxicity of Polysorbate 80 were examined in order to test the possibility that Zn 2+ is involved in cytotoxic action of Polysorbate 80. The surfactant at concentrations of 10 μg/ml or more significantly augmented FluoZin-3 fluorescent in a concentration-dependent manner, indicating an increase in intracellular Zn 2+ concentration. The increase by Polysorbate 80 was also observed after removing extracellular Zn 2+ , suggesting an intracellular Zn 2+ release. The simultaneous application of Polysorbate 80 (30 μg/ml) and ZnCl 2 (10–30 μM) significantly increased cell lethality. The simultaneous application of ZnCl 2 accelerated the process of cell death induced by Polysorbate 80 and the combination increased oxidative stress. Results may indicate that the cytotoxicity of Polysorbate 80 at clinical concentrations is modified by micromolar zinc. Although there is no clinical report that Polysorbate 80 and zinc salt are simultaneously applied to human as far as our knowledge, it may be speculated that zinc induces some diverse actions in cancer treatment with water-insoluble anticancer agent including nanoparticle drug of which the solvent is Polysorbate 80.
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Polysorbate 80 increases the susceptibility to oxidative stress in rat thymocytes.
Toxicology, 2005Co-Authors: Tomoko Tatsuishi, Kyoko Iwase, Masako Kobayashi, Jun-ya Yamaguchi, Yumiko Nishimura, Aimi Kanada, Yasuo Oyama, Seigo HiramaAbstract:Effect of simultaneous application of Polysorbate 80, a nonionic surfactant widely used in pharmaceutical products, and hydrogen peroxide on rat thymocytes was examined to see if Polysorbate 80 increases the susceptibility to oxidative stress because this surfactant decreases the cellular content of glutathione. Polysorbate 80 at clinically-relevant concentrations increases the cytotoxicity of hydrogen peroxide under the in vitro condition. Result suggests that Polysorbate 80 may increase the susceptibility of cells to oxidative stress.
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Flow-cytometric analysis on adverse effects of Polysorbate 80 in rat thymocytes.
Toxicology, 2004Co-Authors: Seigo Hirama, Tomoko Tatsuishi, Kyoko Iwase, Hiromi Nakao, Chisato Umebayashi, Yasutaka Nishizaki, Masako Kobayashi, Yoshiro Okano, Shiro Ishida, Yasuo OyamaAbstract:The effects of Polysorbate 80, a non-ionic surfactant widely used in pharmaceutical products, on rat thymocytes were examined to reveal its toxic property at the cellular level. Polysorbate 80 at concentrations of 1-100 microg/ml did not significantly affect the cell viability. This surfactant at 30 microg/ml or more augmented the intensity of fluo-3 fluorescence, indicating the increase in intracellular Ca(2+) concentration. Such an augmentation of fluo-3 fluorescence by Polysorbate 80 was not seen under the Ca(2+)-free condition, suggesting that Polysorbate 80 increased membrane Ca(2+) permeability. The concentration-dependent Polysorbate 80 at 10 microg/ml or more attenuated the intensity of 5-chloromethylfluorescein, indicating a decrease in cellular content of glutathione by Polysorbate 80. Furthermore, the agent at 1 microg/ml or more attenuated the intensity of bis-(1,3-dibutylbarbituric acid) trimethine oxonol fluorescence, being independent from the changes in membrane potential. This phenomenon indicates that Polysorbate 80 at 1 microg/ml or more may attenuate the incorporation of anionic compounds into the membranes. It can be suggested that Polysorbate 80 modifies some of membranes and intracellular physiological parameters without affecting the cell viability.
Michael A. Carducci - One of the best experts on this subject based on the ideXlab platform.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ping He, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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Simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography/tandem mass spectrometry
Analytical biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:Abstract An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid–liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50×2.1 mm internal diameter) packed with a 3.5-μm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (∼0.4 ng/ml) and 1.00 μg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.
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simultaneous analysis of docetaxel and the formulation vehicle Polysorbate 80 in human plasma by liquid chromatography tandem mass spectrometry
Analytical Biochemistry, 2004Co-Authors: Sharyn D Baker, Jaap Verweij, Michael A. Carducci, Ming Zhao, Alex SparreboomAbstract:An analytical procedure for the simultaneous determination of the anticancer agent docetaxel (Taxotere) and its formulation vehicle Polysorbate 80 (Tween 80) in human plasma samples is described. Sample pretreatment involved a double liquid-liquid extraction step with a mixture of acetonitrile/n-butyl chloride (1/4, v/v). Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a reversed-phase Waters X-Terra mass spectrometry (MS) column (50 x 2.1mm internal diameter) packed with a 3.5-microm octadecyl stationary phase, using isocratic elution. Detection of docetaxel and Polysorbate 80 was performed using tandem MS detection with electrospray ionization. Validation results indicated that the method is accurate and precise and has lower limits of quantitation of 0.500 nM (approximately 0.4 ng/ml) and 1.00 microg/ml for docetaxel and Polysorbate 80, respectively. The method was subsequently used to measure concentrations of docetaxel and Polysorbate 80 in plasma samples in support of a project to assess the influence of Polysorbate 80 concentrations on the disposition and toxicity profile of docetaxel in cancer patients receiving Taxotere.