The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Arai Katsutoshi - One of the best experts on this subject based on the ideXlab platform.
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Non-motile tetraploid spermatozoa of Misgurnus Loach hybrids
'Springer Science and Business Media LLC', 2016Co-Authors: Zhao Yan, Fujimoto Takafumi, Psenicka Martin, Saito Taiju, Arai KatsutoshiAbstract:We have compared various properties of spermatozoa from the wild diploid male Pond Loach Misgurnus anguillicaudatus to those from the interspecific male hybrid of the cross between a female M. anguillicaudatus and a male mud Loach M. mizolepis. Our results show that spermatozoa from this interspecific hybrid had poor motility, low viability, abnormal morphology, a larger volume of mitochondrial mass per cell and higher ATP content of spermatozoa with tetraploid DNA content, and they were present at a low concentration. The interspecific hybrid males produced spermatozoa with a larger head, with either no flagellum (36.4 %), one flagellum (46.7 %) or two flagella (16.9 %). These flagella were shorter than those of the normal wild-type male M. anguillicaudatus and often presented with abnormalities in microtubule structure. An abnormally shorter flagellum has difficulty in propelling tetraploid spermatozoa with an increased head size in normal progressive motility, although they had higher energy, as shown by their larger volume of mitochondrial mass and higher ATP content. These tetraploid spermatozoa are likely produced by the arrest of the regular meiotic division after chromosomal replication, followed by abnormal spermiogenesis
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Heat-shock-induced tetraploid and diploid/tetraploid mosaic in Pond Loach, Misgurnus anguillicaudatus
'Springer Science and Business Media LLC', 2013Co-Authors: Fujimoto Takafumi, Sakao Suzu, Oshima Kouzou, Yamaha Etsuro, Arai KatsutoshiAbstract:Tetraploid fish, which are considered as key resources of diploid gametes for further breeding and ploidy manipulation, can be artificially induced by inhibition of the mitotic cell division with hydrostatic pressure or temperature treatments. Although many attempts have been made to induce artificial tetraploid strains, successful establishment of viable and fertile tetraploid strains are rare. In Pond Loach, Misgurnus anguillicaudatus, natural tetraploid individuals are distributed in wild populations and diploid gametes from the tetraploid fish have been used for the induction of polyploid individuals, but artificially induced tetraploid strains have not been established yet. In the present study, we optimised starting timing of the heat-shock treatment (41 °C for 2 min) to inhibit a mitotic cell division in fertilised eggs of the normal diploid Pond Loach between 21 and 51 min after insemination at 20 °C. After the treatment, we observed external appearance of hatching larvae and flow cytometrically determined ploidy status of the resultant larvae. Although tetraploid and diploid/tetraploid mosaic larvae were obtained, the optimum timings for induction of tetraploidy varied amongst crosses. Various kinds of ploidy such as haploidy, diploidy, triploidy, pentaploidy, hexaploidy, aneuploidy and mosaic were detected in non-optimum heat-shock timings for tetraploidisation. Survivors, a tetraploid and a diploid/tetraploid mosaic male, matured at the age of 1-year-old, but they produced functional haploid spermatozoa
Yaowei Fang - One of the best experts on this subject based on the ideXlab platform.
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effects of dietary lactobacillus helveticus on the growth rate disease resistance and intestinal health of Pond Loach misgurnus anguillicaudatus
Aquaculture, 2021Co-Authors: Guang Yang, Xiaolin Cui, Shu Liu, Xiaoyue Hou, Wenrong Meng, Hongyu Zhang, Wei Zheng, Yaowei FangAbstract:Abstract This study sought to investigate the effects of Lactobacillus helveticus HML037 dietary supplementation on the growth performance, innate immunity, disease resistance and intestinal health of the Pond Loach (Misgurnus anguillicaudatus). Three hundred and sixty Loaches were randomly divided into four experimental diets supplemented with 0 (C group), 1 × 107 (T1 group), 1 × 108 (T2 group), and 1 × 109 (T3 group) CFU g−1 of L. helveticus HML037. After 8 weeks of feeding, the results demonstrated that the growth performance, digestive enzyme activity, the expression levels of immune-related genes and growth-related genes, antioxidant response, and disease resistance against pathogenic bacteria of M. anguillicaudatus were all significantly increased by the diets containing different levels of L. helveticus HML037 compared with the control group, specifically, the optimal dietary L. helveticus HML037 was 1 × 108 CFU g−1. Activation of immunity was consistent with the microbiota data showing that the species richness and diversity of the L. helveticus HML037 addition group were remarkably increased, and the abundance of Lactobacillus was increased, and the abundance of Aeromonas, Acidovorax and Shewanella were decreased. These results indicated that dietary L. helveticus HML037 supplementation could promote growth performance, non-specific immunity and the healthy gut microbiota of M. anguillicaudatus, and therefore may be implemented as an effective Loach dietary supplement.
Fujimoto Takafumi - One of the best experts on this subject based on the ideXlab platform.
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Non-motile tetraploid spermatozoa of Misgurnus Loach hybrids
'Springer Science and Business Media LLC', 2016Co-Authors: Zhao Yan, Fujimoto Takafumi, Psenicka Martin, Saito Taiju, Arai KatsutoshiAbstract:We have compared various properties of spermatozoa from the wild diploid male Pond Loach Misgurnus anguillicaudatus to those from the interspecific male hybrid of the cross between a female M. anguillicaudatus and a male mud Loach M. mizolepis. Our results show that spermatozoa from this interspecific hybrid had poor motility, low viability, abnormal morphology, a larger volume of mitochondrial mass per cell and higher ATP content of spermatozoa with tetraploid DNA content, and they were present at a low concentration. The interspecific hybrid males produced spermatozoa with a larger head, with either no flagellum (36.4 %), one flagellum (46.7 %) or two flagella (16.9 %). These flagella were shorter than those of the normal wild-type male M. anguillicaudatus and often presented with abnormalities in microtubule structure. An abnormally shorter flagellum has difficulty in propelling tetraploid spermatozoa with an increased head size in normal progressive motility, although they had higher energy, as shown by their larger volume of mitochondrial mass and higher ATP content. These tetraploid spermatozoa are likely produced by the arrest of the regular meiotic division after chromosomal replication, followed by abnormal spermiogenesis
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Heat-shock-induced tetraploid and diploid/tetraploid mosaic in Pond Loach, Misgurnus anguillicaudatus
'Springer Science and Business Media LLC', 2013Co-Authors: Fujimoto Takafumi, Sakao Suzu, Oshima Kouzou, Yamaha Etsuro, Arai KatsutoshiAbstract:Tetraploid fish, which are considered as key resources of diploid gametes for further breeding and ploidy manipulation, can be artificially induced by inhibition of the mitotic cell division with hydrostatic pressure or temperature treatments. Although many attempts have been made to induce artificial tetraploid strains, successful establishment of viable and fertile tetraploid strains are rare. In Pond Loach, Misgurnus anguillicaudatus, natural tetraploid individuals are distributed in wild populations and diploid gametes from the tetraploid fish have been used for the induction of polyploid individuals, but artificially induced tetraploid strains have not been established yet. In the present study, we optimised starting timing of the heat-shock treatment (41 °C for 2 min) to inhibit a mitotic cell division in fertilised eggs of the normal diploid Pond Loach between 21 and 51 min after insemination at 20 °C. After the treatment, we observed external appearance of hatching larvae and flow cytometrically determined ploidy status of the resultant larvae. Although tetraploid and diploid/tetraploid mosaic larvae were obtained, the optimum timings for induction of tetraploidy varied amongst crosses. Various kinds of ploidy such as haploidy, diploidy, triploidy, pentaploidy, hexaploidy, aneuploidy and mosaic were detected in non-optimum heat-shock timings for tetraploidisation. Survivors, a tetraploid and a diploid/tetraploid mosaic male, matured at the age of 1-year-old, but they produced functional haploid spermatozoa
Katsutoshi Arai - One of the best experts on this subject based on the ideXlab platform.
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heat shock induced tetraploid and diploid tetraploid mosaic in Pond Loach misgurnus anguillicaudatus
Aquaculture International, 2013Co-Authors: Takafumi Fujimoto, Suzu Sakao, Kouzou Oshima, Etsuro Yamaha, Katsutoshi AraiAbstract:Tetraploid fish, which are considered as key resources of diploid gametes for further breeding and ploidy manipulation, can be artificially induced by inhibition of the mitotic cell division with hydrostatic pressure or temperature treatments. Although many attempts have been made to induce artificial tetraploid strains, successful establishment of viable and fertile tetraploid strains are rare. In Pond Loach, Misgurnus anguillicaudatus, natural tetraploid individuals are distributed in wild populations and diploid gametes from the tetraploid fish have been used for the induction of polyploid individuals, but artificially induced tetraploid strains have not been established yet. In the present study, we optimised starting timing of the heat-shock treatment (41 °C for 2 min) to inhibit a mitotic cell division in fertilised eggs of the normal diploid Pond Loach between 21 and 51 min after insemination at 20 °C. After the treatment, we observed external appearance of hatching larvae and flow cytometrically determined ploidy status of the resultant larvae. Although tetraploid and diploid/tetraploid mosaic larvae were obtained, the optimum timings for induction of tetraploidy varied amongst crosses. Various kinds of ploidy such as haploidy, diploidy, triploidy, pentaploidy, hexaploidy, aneuploidy and mosaic were detected in non-optimum heat-shock timings for tetraploidisation. Survivors, a tetraploid and a diploid/tetraploid mosaic male, matured at the age of 1-year-old, but they produced functional haploid spermatozoa.
Weimin Wang - One of the best experts on this subject based on the ideXlab platform.
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cloning characterization antibacterial activity and expression of hamp in Pond Loach misgurnus anguillicaudatus after bacterial challenge with aeromonas hydrophila
Aquaculture, 2019Co-Authors: Supranee Ruenkoed, Weimin WangAbstract:Abstract Loaches are popular in aquaculture in China due to their flesh having a high concentration of nutrients important for human health, such as amino acids, vitamins, minerals, and taurine. As such, they are used in Chinese traditional medicine to restore health and slow aging. However, the quick expansion of intensive Loach culture to meet consumer demand has been accompanied by the increased prevalence of disease outbreaks, most often caused by Aeromonas spp. bacteria. We collected and identified pathogenic bacteria causing hemorrhagic septicemia in Pond Loach sourced from two provinces of China (Hubei and Hunan), and found three Aeromonas species: hydrophila (the most prevalent), sobria and veronii. We performed a bacterial challenge on the Pond Loach (Misgurnus anguillicaudatus) using the different concentrations of A. hydrophila in order to determine the median lethal dose, and found that the LD50 value was 2.14 × 107 CFU/fish. Hepcidin protein encoded by hepcidin antimicrobial peptide (hamp) gene is a key factor for iron regulation, enhancement of the host's innate immune system and acts as antimicrobial peptide. To better understand the functions of this protein in Pond Loach, we cloned it, conducted in silico physicochemical characterization, and studied its expression at the mRNA level both in healthy Loach, and after a challenge with A. hydrophila. We found two very similar paralogs (a and b) of the hamp 1 gene, the open reading frames of which were 276 bp, both encoding an identical 91 amino acid putative peptide. The purified recombinant hepcidin protein exhibited antibacterial activity against all six studied bacterial species at 100–3200 arbitrary units/ml and can decrease 50% of the cumulation mortality of Loaches which were injected by A. hydrophila. Both hamp 1 paralogs were basally expressed in most tissues, and significantly up-regulated in the liver, spleen and kidney after the bacterial challenge. The results of this study strongly indicate that hepcidin plays a role in defense mechanisms against pathogenic bacteria and inflammation in Loaches.