The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform

Marcos Fábio Gadelha Rocha - One of the best experts on this subject based on the ideXlab platform.

  • subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for malassezia pachydermatis
    Journal of Microbiological Methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 microg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.

  • Subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for Malassezia pachydermatis.
    Journal of microbiological methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of

Rossana De Aguiar Cordeiro - One of the best experts on this subject based on the ideXlab platform.

  • subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for malassezia pachydermatis
    Journal of Microbiological Methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 microg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.

  • Subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for Malassezia pachydermatis.
    Journal of microbiological methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of

M.r. Prado - One of the best experts on this subject based on the ideXlab platform.

  • subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for malassezia pachydermatis
    Journal of Microbiological Methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 microg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.

  • Subculture on Potato Dextrose Agar as a complement to the broth microdilution assay for Malassezia pachydermatis.
    Journal of microbiological methods, 2008
    Co-Authors: M.r. Prado, Erika H.s. Brito, Raimunda Sâmia Nogueira Brilhante, Rossana De Aguiar Cordeiro, João Jaime Giffoni Leite, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
    Abstract:

    The main aim of this study was to verify the efficacy of subculture on Potato Dextrose Agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of

Lorne M. Golub - One of the best experts on this subject based on the ideXlab platform.

  • Potato Dextrose Agar Antifungal Susceptibility Testing for Yeasts and Molds: Evaluation of Phosphate Effect on Antifungal Activity of CMT-3
    Antimicrobial agents and chemotherapy, 2002
    Co-Authors: Yu Liu, G.t. Tortora, Maria Emanuel Ryan, Hsi-ming Lee, Lorne M. Golub
    Abstract:

    The broth macrodilution method (BMM) for antifungal susceptibility testing, approved by the National Committee for Clinical Laboratory Standards (NCCLS), was found to have deficiencies in testing of the antifungal activity of a new type of antifungal agent, a nonantibacterial chemically modified tetracycline (CMT-3). The high content of phosphate in the medium was found to greatly increase the MICs of CMT-3. To avoid the interference of phosphate in the test, a new method using Potato Dextrose Agar (PDA) as a culture medium was developed. Eight strains of fungi, including five American Type Culture Collection strains and three clinical isolates, were used to determine the MICs of amphotericin B and itraconazole with both the BMM and the PDA methods. The MICs of the two antifungal agents determined with the PDA method showed 99% agreement with those determined with the BMM method within 1 log2 dilution. Similarly, the overall reproducibility of the MICs with the PDA method was above 97%. Three other antifungal agents, fluconazole, ketoconazole, and CMT-3, were also tested in parallel against yeasts and molds with both the BMM and the PDA methods. The MICs of fluconazole and ketoconazole determined with the PDA method showed 100% agreement within 1 log2 dilution of those obtained with the BMM method. However, the MICs of CMT-3 determined with the BMM method were as high as 128 times those determined with the PDA method. The effect of phosphate on the antifungal activity of CMT-3 was evaluated by adding Na2HPO4 to PDA in the new method. It was found that the MIC of CMT-3 against a Penicillium sp. increased from 0.5 μg/ml (control) to 2.0 μg/ml when the added phosphate was used at a concentration of 0.8 mg/ml, indicating a strong interference of Na2HPO4 with the antifungal activity of CMT-3. Except for fluconazole, all the other antifungal agents demonstrated clear end points among the yeasts and molds tested. Nevertheless, with its high reproducibility, good agreement with NCCLS proposed MIC ranges, and lack of interference of phosphate, the PDA method shows promise as a useful assay for antifungal susceptibility testing and screening for new antifungal agents, especially for drugs that may be affected by high (supraphysiologic) phosphate concentrations.

William T. Perkins - One of the best experts on this subject based on the ideXlab platform.

  • Copper deficiency in Potato Dextrose Agar causes reduced pigmentation in cultures of various fungi
    FEMS microbiology letters, 2007
    Co-Authors: Garth W. Griffith, Gary L. Easton, Andrew P. Detheridge, Kevin M Roderick, Arwyn Edwards, Hilary J. Worgan, Jean Nicholson, William T. Perkins
    Abstract:

    Potato Dextrose Agar (PDA) is one of the most commonly used media for the isolation and cultivation of fungi, with morphological features and pigmentation in culture often being important for identification of cultures. Cultivation of various fungi on different brands and batches of powdered (commercial) Potato Dextrose media revealed deficient pigmentation in five of 10 media tested. Reduced pigmentation on these media was correlated with low levels of copper and colony colour was restored by the addition of copper. Deficient pigmentation was most pronounced when copper levels in the medium were below 50ngmL � 1 (50p.p.b.; 0.8mM). Differences in pigmentation and laccase activity of spore and mycelial preparations were quantified for representative species belonging to the genera Aspergillus, Fusarium, Trichoderma, Cladosporium and Penicillium grown on PDA containing different amounts of copper. A strong positive correlation between laccase activity and copper levels was observed. Differences were also found between batches of raw Potatoes, with organically cultivated tubers having higher copper levels than those grown by conventional methods, possibly because of the use of copper-based fungicides in the former case. Routine addition of 1000ngmL � 1 copper (or standard trace element solutions) to PDA and other undefined media is advised to avoid atypical culture pigmentation and possibly other consequences of reduced activity of copper-requiring enzymes.