The Experts below are selected from a list of 5835 Experts worldwide ranked by ideXlab platform
Bo Chuan Wang - One of the best experts on this subject based on the ideXlab platform.
-
ITS and trnL-F Sequences analysis of Potentilla discolor Bge.
Proceedings of the 3rd International Conference on Material Mechanical and Manufacturing Engineering, 2015Co-Authors: Shao Xuan Zhang, Yarong Li, Tiantian Wang, Bo Chuan WangAbstract:To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Potentilla discolor Bge., we extracted the genome DNA from the leaves of Potentilla discolor Bge., purchased from Hebei Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
Sequences Analysis of ITS and Trnl-F Regions in Potentilla Fragarioides L
2015 8th International Conference on Intelligent Computation Technology and Automation (ICICTA), 2015Co-Authors: Shao Xuan Zhang, Tiantian Wang, Bo Chuan WangAbstract:For identification of Potentilla fragarioides L., scientific data of the internal transcribed spacer (ITS) and chloroplast trnL-F sequences are needed. So in this study, we extracted the genome DNA from the leaves of Potentilla fragarioides L. Collected in Jilin Province, amplified the ITS and chloroplast trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
ITS and trnL-F Sequences Analysis of Potentilla bifurca L var. humilior Rupr.
2015 Seventh International Conference on Measuring Technology and Mechatronics Automation, 2015Co-Authors: Shao Xuan Zhang, Bo Chuan Wang, Tiantian WangAbstract:To provide scientific data of the internal transcribed spacer (ITS) and chloroplast trn L-F sequences for identification of Potentilla bifurca L var. Humilior Rupr., we extracted the genome DNA from the leaves of Potentilla bifurca L var. Humilior Rupr. Collected in Jilin Province, amplified the ITS and chloroplast trn L-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
comparison of chloroplast trnl f sequences of 5 common Potentilla species in jilin province of china
Advanced Materials Research, 2012Co-Authors: Shao Xuan Zhang, Yun Hui Ling, Bo Chuan WangAbstract:To find the differences in the trnL-F sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. We found that the trnL-F sequences of different species of Potentilla are significantly different. So trn-L sequence analysis and other methods derived from it can be used in authentication of Potentilla.
-
comparison of the its sequences of 5 common Potentilla species in jilin province of china
Advanced Materials Research, 2012Co-Authors: Shao Xuan Zhang, Bo Chuan Wang, Yun Hui LingAbstract:To find the differences in the internal transcribed spacer(ITS) sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis and the sequence data suggested that our samples of this species might be related to hybridization. Other 4 species showed intraspecies-stability in ITS sequence. The ITS sequences of these 5 Potentilla species are significantly different. So ITS sequence analysis and other methods derived from it can be used in authentication of Potentilla.
Shao Xuan Zhang - One of the best experts on this subject based on the ideXlab platform.
-
ITS and trnL-F Sequences analysis of Potentilla discolor Bge.
Proceedings of the 3rd International Conference on Material Mechanical and Manufacturing Engineering, 2015Co-Authors: Shao Xuan Zhang, Yarong Li, Tiantian Wang, Bo Chuan WangAbstract:To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Potentilla discolor Bge., we extracted the genome DNA from the leaves of Potentilla discolor Bge., purchased from Hebei Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
Sequences Analysis of ITS and Trnl-F Regions in Potentilla Fragarioides L
2015 8th International Conference on Intelligent Computation Technology and Automation (ICICTA), 2015Co-Authors: Shao Xuan Zhang, Tiantian Wang, Bo Chuan WangAbstract:For identification of Potentilla fragarioides L., scientific data of the internal transcribed spacer (ITS) and chloroplast trnL-F sequences are needed. So in this study, we extracted the genome DNA from the leaves of Potentilla fragarioides L. Collected in Jilin Province, amplified the ITS and chloroplast trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
ITS and trnL-F Sequences Analysis of Potentilla bifurca L var. humilior Rupr.
2015 Seventh International Conference on Measuring Technology and Mechatronics Automation, 2015Co-Authors: Shao Xuan Zhang, Bo Chuan Wang, Tiantian WangAbstract:To provide scientific data of the internal transcribed spacer (ITS) and chloroplast trn L-F sequences for identification of Potentilla bifurca L var. Humilior Rupr., we extracted the genome DNA from the leaves of Potentilla bifurca L var. Humilior Rupr. Collected in Jilin Province, amplified the ITS and chloroplast trn L-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.
-
comparison of chloroplast trnl f sequences of 5 common Potentilla species in jilin province of china
Advanced Materials Research, 2012Co-Authors: Shao Xuan Zhang, Yun Hui Ling, Bo Chuan WangAbstract:To find the differences in the trnL-F sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. We found that the trnL-F sequences of different species of Potentilla are significantly different. So trn-L sequence analysis and other methods derived from it can be used in authentication of Potentilla.
-
comparison of the its sequences of 5 common Potentilla species in jilin province of china
Advanced Materials Research, 2012Co-Authors: Shao Xuan Zhang, Bo Chuan Wang, Yun Hui LingAbstract:To find the differences in the internal transcribed spacer(ITS) sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis and the sequence data suggested that our samples of this species might be related to hybridization. Other 4 species showed intraspecies-stability in ITS sequence. The ITS sequences of these 5 Potentilla species are significantly different. So ITS sequence analysis and other methods derived from it can be used in authentication of Potentilla.
Dengwu Li - One of the best experts on this subject based on the ideXlab platform.
-
phytochemical profiles antioxidant and antimicrobial activities of three Potentilla species
BMC Complementary and Alternative Medicine, 2013Co-Authors: Shanshan Wang, Dongmei Wang, Wenjun Pu, Dengwu LiAbstract:Extracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species (Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia) in order to discover new resources for lead structures and pharmaceutical products. Chemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride/chloranil-based method (SBC); Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method. The results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 (P. fruticosa), 2.56 (P. glabra) and 2.68 mg/g (P. parvifolia), respectively. The highest content of total identified phenolic compounds (hyperoside, (+)-catechin, caffeic acid, ferulic acid, rutin and ellagic acid) was observed in P. parvifolia (14.17 mg/g), follow by P. fruticosa (10.01 mg/g) and P. glabra (7.01 mg/g). P. fruticosa possessed the highest content of total phenolic (84.93 ± 0.50 mmol gallic acid equivalent/100 g) and total flavonoid (84.14 ± 0.03 mmol quercetin equivalent/100 g), which were in good correlation with its significant DPPHIC50 (16.87 μg/mL), ABTS (2763.48 μmol Trolox equivalent/g) and FRAP (1398.70 μmol Trolox equivalent/g) capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg/mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC50 and MIC values of 20.52–47.02 mg/mL and 0.78–50 mg/mL, respectively. These results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.
Duan Hong-quan - One of the best experts on this subject based on the ideXlab platform.
-
The determination of Potentilla flavone from Potentilla chinesis in different growth periods and parts by HPLC
Journal of Tianjin Medical University, 2020Co-Authors: Duan Hong-quanAbstract:Objective:To develop an HPLC method for determination of Potentilla flavone from Potentilla chinesis in different growth periods and growth sites.Methods:Kromasil-C18 was used as analysis column with methanol-3‰ acetic acid water(49∶51) as mobile phase.The flow rate was 1.0 ml.min-1 and the determination wavelength was 254 nm.Results:The calibration curve of Potentilla flavone was linear within the range of 5~150 μg.ml-1,the r value was 0.999 9;and its average recovery was 101.21%.The highest content of Potentilla flavone are in July and leaves of P.chinesis,with the contents are 1.018‰ and 1.477‰,respectively.Conclusion:This method is simple,can be used for the content analysis of Potentilla flavone in Potentilla chinesis.
-
Study on absorption of Potentilla flavone in isolated rat small intestine utilizing everted-gut technique
Journal of Tianjin Medical University, 2020Co-Authors: Duan Hong-quanAbstract:Objective:To study the absorption of Potentilla flavone in isolated rat small intestine.Methods:Everted-gut technique was utilized to study the absorption properties of Potentilla flavone at three different concentration range (high range,middle range and low range).Results:The absorption of Potentilla flavone increased according to their concentration in a definite concentration range,as well as a concentration-depent.Conclution:The pharmacokinetics of gut absorption studies showes the absorption of Potentilla flavone fits to linear absorption model and the passive transport features.
-
Isolation Process of Anti-diabetes Constituents from Potentilla chinesis
Lishizhen Medicine and Materia Medica Research, 2020Co-Authors: Duan Hong-quanAbstract:Objective To study the technological parameters of isolation of anti-diabetes constituents,Potentilla flavone from Potentilla chinesis.MethodsThe separation process was established through evaluation of separation performance and the optimal separation conditions of macroporous absorption resin and polyamide.ResultsAB-8 Macroporous Resin and polyamide were chosen for isolation and purification from Potentilla chinesis.ConclusionThe extraction process is stable and suitable for industry.
Woochan Kwon - One of the best experts on this subject based on the ideXlab platform.
-
The complete chloroplast genome of Potentilla Freyniana Bornm. (Rosaceae)
Mitochondrial DNA Part B, 2019Co-Authors: Jongsun Park, Woochan KwonAbstract:Potentilla freyniana Bornm. has radical leaves with petioles and trifoliolate distinguished from Potentilla fragarioides and Duchesnea indica. In this study, we presented first complete chloroplast...
-
The complete chloroplast genome of Potentilla stolonifera var. quelpaertensis Nakai
Mitochondrial DNA Part B, 2019Co-Authors: Jongsun Park, Woochan KwonAbstract:Potentilla stolonifera var. quelpaertensis Nakai has developed stolon in comparison to Potentilla fragarioides var. major. To uncover phylogenetic relations of two species, we presented complete ch...
