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Shohei Sakuda - One of the best experts on this subject based on the ideXlab platform.
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Precocene II a trichothecene production inhibitor binds to voltage dependent anion channel and increases the superoxide level in mitochondria of fusarium graminearum
PLOS ONE, 2015Co-Authors: Tomohiro Furukawa, Naoko Sakamoto, Michio Suzuki, Makoto Kimura, Hiromichi Nagasawa, Shohei SakudaAbstract:Precocene II, a constituent of essential oils, shows antijuvenile hormone activity in insects and inhibits trichothecene production in fungi. We investigated the molecular mechanism by which Precocene II inhibits trichothecene production in Fusarium graminearum, the main causal agent of Fusarium head blight and trichothecene contamination in grains. Voltage-dependent anion channel (VDAC), a mitochondrial outer membrane protein, was identified as the Precocene II-binding protein by an affinity magnetic bead method. Precocene II increased the superoxide level in mitochondria as well as the amount of oxidized mitochondrial proteins. Ascorbic acid, glutathione, and α-tocopherol promoted trichothecene production by the fungus. These antioxidants compensated for the inhibitory activity of Precocene II on trichothecene production. These results suggest that the binding of Precocene II to VDAC may cause high superoxide levels in mitochondria, which leads to stopping of trichothecene production.
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correlation of atp citrate lyase and acetyl coa levels with trichothecene production in fusarium graminearum
Toxins, 2013Co-Authors: Naoko Sakamoto, Tomohiro Furukawa, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Jermnak Usuma, Shohei SakudaAbstract:The correlation of ATP citrate lyase (ACL) and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor (Precocene II) and an enhancer (cobalt chloride) of trichothecene production by changing carbon sources in liquid medium. When Precocene II (30 µM) was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of Precocene II (300 µM) together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that Precocene II may affect a step down-stream of the target of cobalt chloride.
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Isolation and identification of Precocenes and piperitone from essential oils as specific inhibitors of trichothecene production by Fusarium graminearum.
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Atsushi Yaguchi, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Haruo Takahashi, Takashi Nakajima, Yoshiko Sugita-konishi, Shohei SakudaAbstract:Inhibitors of deoxynivalenol production by Fusarium graminearum are useful for protecting crops from deoxynivalenol contamination. We isolated Precocenes and piperitone from the essential oils of Matricaria recutita and Eucalyptus dives, respectively, as specific inhibitors of the production of 3-acetyldeoxynivalenol, a biosynthetic precursor of deoxynivalenol. Precocenes I and II and piperitone inhibited 3-acetyldeoxynivalenol production by F. graminearum in a liquid culture with IC50 values of 16.6, 1.2, and 306 μM, respectively, without inhibiting fungal growth. Precocene II also inhibited deoxynivalenol production by the fungus in a solid culture on rice with an IC50 value of 2.0 ppm. Precocene II and piperitone decreased the mRNA levels of Tri4, Tri5, Tri6, and Tri10 encoding proteins required for deoxynivalenol biosynthesis.
Tomohiro Furukawa - One of the best experts on this subject based on the ideXlab platform.
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Precocene II a trichothecene production inhibitor binds to voltage dependent anion channel and increases the superoxide level in mitochondria of fusarium graminearum
PLOS ONE, 2015Co-Authors: Tomohiro Furukawa, Naoko Sakamoto, Michio Suzuki, Makoto Kimura, Hiromichi Nagasawa, Shohei SakudaAbstract:Precocene II, a constituent of essential oils, shows antijuvenile hormone activity in insects and inhibits trichothecene production in fungi. We investigated the molecular mechanism by which Precocene II inhibits trichothecene production in Fusarium graminearum, the main causal agent of Fusarium head blight and trichothecene contamination in grains. Voltage-dependent anion channel (VDAC), a mitochondrial outer membrane protein, was identified as the Precocene II-binding protein by an affinity magnetic bead method. Precocene II increased the superoxide level in mitochondria as well as the amount of oxidized mitochondrial proteins. Ascorbic acid, glutathione, and α-tocopherol promoted trichothecene production by the fungus. These antioxidants compensated for the inhibitory activity of Precocene II on trichothecene production. These results suggest that the binding of Precocene II to VDAC may cause high superoxide levels in mitochondria, which leads to stopping of trichothecene production.
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correlation of atp citrate lyase and acetyl coa levels with trichothecene production in fusarium graminearum
Toxins, 2013Co-Authors: Naoko Sakamoto, Tomohiro Furukawa, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Jermnak Usuma, Shohei SakudaAbstract:The correlation of ATP citrate lyase (ACL) and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor (Precocene II) and an enhancer (cobalt chloride) of trichothecene production by changing carbon sources in liquid medium. When Precocene II (30 µM) was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of Precocene II (300 µM) together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that Precocene II may affect a step down-stream of the target of cobalt chloride.
Naoko Sakamoto - One of the best experts on this subject based on the ideXlab platform.
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Precocene II a trichothecene production inhibitor binds to voltage dependent anion channel and increases the superoxide level in mitochondria of fusarium graminearum
PLOS ONE, 2015Co-Authors: Tomohiro Furukawa, Naoko Sakamoto, Michio Suzuki, Makoto Kimura, Hiromichi Nagasawa, Shohei SakudaAbstract:Precocene II, a constituent of essential oils, shows antijuvenile hormone activity in insects and inhibits trichothecene production in fungi. We investigated the molecular mechanism by which Precocene II inhibits trichothecene production in Fusarium graminearum, the main causal agent of Fusarium head blight and trichothecene contamination in grains. Voltage-dependent anion channel (VDAC), a mitochondrial outer membrane protein, was identified as the Precocene II-binding protein by an affinity magnetic bead method. Precocene II increased the superoxide level in mitochondria as well as the amount of oxidized mitochondrial proteins. Ascorbic acid, glutathione, and α-tocopherol promoted trichothecene production by the fungus. These antioxidants compensated for the inhibitory activity of Precocene II on trichothecene production. These results suggest that the binding of Precocene II to VDAC may cause high superoxide levels in mitochondria, which leads to stopping of trichothecene production.
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correlation of atp citrate lyase and acetyl coa levels with trichothecene production in fusarium graminearum
Toxins, 2013Co-Authors: Naoko Sakamoto, Tomohiro Furukawa, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Jermnak Usuma, Shohei SakudaAbstract:The correlation of ATP citrate lyase (ACL) and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor (Precocene II) and an enhancer (cobalt chloride) of trichothecene production by changing carbon sources in liquid medium. When Precocene II (30 µM) was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of Precocene II (300 µM) together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that Precocene II may affect a step down-stream of the target of cobalt chloride.
Hiromichi Nagasawa - One of the best experts on this subject based on the ideXlab platform.
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Precocene II a trichothecene production inhibitor binds to voltage dependent anion channel and increases the superoxide level in mitochondria of fusarium graminearum
PLOS ONE, 2015Co-Authors: Tomohiro Furukawa, Naoko Sakamoto, Michio Suzuki, Makoto Kimura, Hiromichi Nagasawa, Shohei SakudaAbstract:Precocene II, a constituent of essential oils, shows antijuvenile hormone activity in insects and inhibits trichothecene production in fungi. We investigated the molecular mechanism by which Precocene II inhibits trichothecene production in Fusarium graminearum, the main causal agent of Fusarium head blight and trichothecene contamination in grains. Voltage-dependent anion channel (VDAC), a mitochondrial outer membrane protein, was identified as the Precocene II-binding protein by an affinity magnetic bead method. Precocene II increased the superoxide level in mitochondria as well as the amount of oxidized mitochondrial proteins. Ascorbic acid, glutathione, and α-tocopherol promoted trichothecene production by the fungus. These antioxidants compensated for the inhibitory activity of Precocene II on trichothecene production. These results suggest that the binding of Precocene II to VDAC may cause high superoxide levels in mitochondria, which leads to stopping of trichothecene production.
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correlation of atp citrate lyase and acetyl coa levels with trichothecene production in fusarium graminearum
Toxins, 2013Co-Authors: Naoko Sakamoto, Tomohiro Furukawa, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Jermnak Usuma, Shohei SakudaAbstract:The correlation of ATP citrate lyase (ACL) and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor (Precocene II) and an enhancer (cobalt chloride) of trichothecene production by changing carbon sources in liquid medium. When Precocene II (30 µM) was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of Precocene II (300 µM) together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that Precocene II may affect a step down-stream of the target of cobalt chloride.
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Isolation and identification of Precocenes and piperitone from essential oils as specific inhibitors of trichothecene production by Fusarium graminearum.
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Atsushi Yaguchi, Hiromichi Nagasawa, Rie Tsuyuki, Tomoya Yoshinari, Haruo Takahashi, Takashi Nakajima, Yoshiko Sugita-konishi, Shohei SakudaAbstract:Inhibitors of deoxynivalenol production by Fusarium graminearum are useful for protecting crops from deoxynivalenol contamination. We isolated Precocenes and piperitone from the essential oils of Matricaria recutita and Eucalyptus dives, respectively, as specific inhibitors of the production of 3-acetyldeoxynivalenol, a biosynthetic precursor of deoxynivalenol. Precocenes I and II and piperitone inhibited 3-acetyldeoxynivalenol production by F. graminearum in a liquid culture with IC50 values of 16.6, 1.2, and 306 μM, respectively, without inhibiting fungal growth. Precocene II also inhibited deoxynivalenol production by the fungus in a solid culture on rice with an IC50 value of 2.0 ppm. Precocene II and piperitone decreased the mRNA levels of Tri4, Tri5, Tri6, and Tri10 encoding proteins required for deoxynivalenol biosynthesis.
Rania A Salama - One of the best experts on this subject based on the ideXlab platform.
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toxicological and biochemical effects of Precocene II against cotton leafworm spodoptera littoralis boisd
Egyptian Journal of Agricultural Research, 2019Co-Authors: Abdelgawad A Fahmi, Mahmoud I Nassar, Erian S Mansour, Anwaar M Abaza, Rania A SalamaAbstract:The cotton leafworm, Spodoptera littoralis (Boisd.) is very polyphagous insect against the most economic plants all over the world. The traditional synthetic chemical insecticides had dramatic effect on the living organisms including man. Anti-juvenile hormones; Precocene II effect on the cotton leafworm, Spodoptera littoralis were assayed under laboratory conditions. (PrecoceneII (6,7-dimethoxy-2,2dimethylchromene) was the main constituent isolated from Ageratum houstonianum plant. The compound caused toxicity against the larvae with (LC10 was 5.59 mg/l), (LC25 was 24.69 mg/l) and (LC50 was 128.53 mg/l). There was some morphogenic effect obtained due to effect of PrecoceneII on larvae, pupae and adult stages. On the other hands the biochemical analysis of the cotton leafworm revealed that Precocene II caused significant increase in chitinase, protease and total carbohydrates with (17.32, 50.17 and 36.85 %), respectively comparison to control.