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Myeong Hee Moon - One of the best experts on this subject based on the ideXlab platform.

  • A soft Preparative Method for membrane proteome analysis using frit inlet asymmetrical flow field-flow fractionation: application in a prostatic cancer cell line.
    Journal of proteome research, 2009
    Co-Authors: Dukjin Kang, Jong Shin Yoo, Myeong Ok Kim, Myeong Hee Moon
    Abstract:

    Membrane proteins participate in a number of important biological functions such as signal transduction, molecular transport, and cell-cell interactions. However, due to the nature of membrane proteins, the development of a Preparative Method that produces a sufficient yield of purified membrane proteins from the cell remains a challenge. In the present study, frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) was employed to fractionate membrane fragments containing membrane proteins from free cytoplasmic proteins of prostatic cancer cell (DU145 cell) lysates. The isolated membrane proteins were then digested and analyzed by nanoflow liquid chromatography/tandem mass spectrometry (nLC-ESI-MS-MS). Since fractionation of the cell lysate mixtures containing membrane fragments and cytoplasmic proteins could be achieved based on the differences of their sizes in FI-AFlFFF, membrane fragments were partially isolated from the cytoplasmic proteins and collected. The performance of FI-AFlFFF for prefractionation of the membrane proteome was examined by comparing the number of membrane proteins that were identified with the number identified using an ultracentrifugation Method. The application of FI-AFlFFF to membrane proteomics produced an increased yield of purified membrane proteins with fewer cytoplasmic proteins compared to a conventional ultracentrifugation Method.

  • a soft Preparative Method for membrane proteome analysis using frit inlet asymmetrical flow field flow fractionation application in a prostatic cancer cell line
    Journal of Proteome Research, 2009
    Co-Authors: Dukjin Kang, Myeong Hee Moon
    Abstract:

    Membrane proteins participate in a number of important biological functions such as signal transduction, molecular transport, and cell−cell interactions. However, due to the nature of membrane proteins, the development of a Preparative Method that produces a sufficient yield of purified membrane proteins from the cell remains a challenge. In the present study, frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) was employed to fractionate membrane fragments containing membrane proteins from free cytoplasmic proteins of prostatic cancer cell (DU145 cell) lysates. The isolated membrane proteins were then digested and analyzed by nanoflow liquid chromatography/tandem mass spectrometry (nLC-ESI-MS-MS). Since fractionation of the cell lysate mixtures containing membrane fragments and cytoplasmic proteins could be achieved based on the differences of their sizes in FI-AFlFFF, membrane fragments were partially isolated from the cytoplasmic proteins and collected. The performance of FI-AFlFFF for pr...

Dukjin Kang - One of the best experts on this subject based on the ideXlab platform.

  • A soft Preparative Method for membrane proteome analysis using frit inlet asymmetrical flow field-flow fractionation: application in a prostatic cancer cell line.
    Journal of proteome research, 2009
    Co-Authors: Dukjin Kang, Jong Shin Yoo, Myeong Ok Kim, Myeong Hee Moon
    Abstract:

    Membrane proteins participate in a number of important biological functions such as signal transduction, molecular transport, and cell-cell interactions. However, due to the nature of membrane proteins, the development of a Preparative Method that produces a sufficient yield of purified membrane proteins from the cell remains a challenge. In the present study, frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) was employed to fractionate membrane fragments containing membrane proteins from free cytoplasmic proteins of prostatic cancer cell (DU145 cell) lysates. The isolated membrane proteins were then digested and analyzed by nanoflow liquid chromatography/tandem mass spectrometry (nLC-ESI-MS-MS). Since fractionation of the cell lysate mixtures containing membrane fragments and cytoplasmic proteins could be achieved based on the differences of their sizes in FI-AFlFFF, membrane fragments were partially isolated from the cytoplasmic proteins and collected. The performance of FI-AFlFFF for prefractionation of the membrane proteome was examined by comparing the number of membrane proteins that were identified with the number identified using an ultracentrifugation Method. The application of FI-AFlFFF to membrane proteomics produced an increased yield of purified membrane proteins with fewer cytoplasmic proteins compared to a conventional ultracentrifugation Method.

  • a soft Preparative Method for membrane proteome analysis using frit inlet asymmetrical flow field flow fractionation application in a prostatic cancer cell line
    Journal of Proteome Research, 2009
    Co-Authors: Dukjin Kang, Myeong Hee Moon
    Abstract:

    Membrane proteins participate in a number of important biological functions such as signal transduction, molecular transport, and cell−cell interactions. However, due to the nature of membrane proteins, the development of a Preparative Method that produces a sufficient yield of purified membrane proteins from the cell remains a challenge. In the present study, frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) was employed to fractionate membrane fragments containing membrane proteins from free cytoplasmic proteins of prostatic cancer cell (DU145 cell) lysates. The isolated membrane proteins were then digested and analyzed by nanoflow liquid chromatography/tandem mass spectrometry (nLC-ESI-MS-MS). Since fractionation of the cell lysate mixtures containing membrane fragments and cytoplasmic proteins could be achieved based on the differences of their sizes in FI-AFlFFF, membrane fragments were partially isolated from the cytoplasmic proteins and collected. The performance of FI-AFlFFF for pr...

J.m. Molina - One of the best experts on this subject based on the ideXlab platform.

  • aluminum diamond composites a Preparative Method to characterize reactivity and selectivity at the interface
    Scripta Materialia, 2012
    Co-Authors: I.e. Monje, Enrique Louis, J.m. Molina
    Abstract:

    Reactivity at the interface in aluminum/diamond composites, which are excellent candidates for thermal management, has been identified to be determinant of their final properties. In this contribution a new Preparative Method consisting of electrochemical etching of the metal and spray cleaning, allows microstructural characterization of the interface. This Methodology preserves interfacial reaction products and highlights the importance of a proper analysis of both fabrication parameters and sample preparation before any conclusion on reactive selectivity at the interface can be reached.

  • Aluminum/diamond composites: A Preparative Method to characterize reactivity and selectivity at the interface
    Scripta Materialia, 2012
    Co-Authors: I.e. Monje, Enrique Louis, J.m. Molina
    Abstract:

    Reactivity at the interface in aluminum/diamond composites, which are excellent candidates for thermal management, has been identified to be determinant of their final properties. In this contribution a new Preparative Method consisting of electrochemical etching of the metal and spray cleaning, allows microstructural characterization of the interface. This Methodology preserves interfacial reaction products and highlights the importance of a proper analysis of both fabrication parameters and sample preparation before any conclusion on reactive selectivity at the interface can be reached.

I.e. Monje - One of the best experts on this subject based on the ideXlab platform.

  • aluminum diamond composites a Preparative Method to characterize reactivity and selectivity at the interface
    Scripta Materialia, 2012
    Co-Authors: I.e. Monje, Enrique Louis, J.m. Molina
    Abstract:

    Reactivity at the interface in aluminum/diamond composites, which are excellent candidates for thermal management, has been identified to be determinant of their final properties. In this contribution a new Preparative Method consisting of electrochemical etching of the metal and spray cleaning, allows microstructural characterization of the interface. This Methodology preserves interfacial reaction products and highlights the importance of a proper analysis of both fabrication parameters and sample preparation before any conclusion on reactive selectivity at the interface can be reached.

  • Aluminum/diamond composites: A Preparative Method to characterize reactivity and selectivity at the interface
    Scripta Materialia, 2012
    Co-Authors: I.e. Monje, Enrique Louis, J.m. Molina
    Abstract:

    Reactivity at the interface in aluminum/diamond composites, which are excellent candidates for thermal management, has been identified to be determinant of their final properties. In this contribution a new Preparative Method consisting of electrochemical etching of the metal and spray cleaning, allows microstructural characterization of the interface. This Methodology preserves interfacial reaction products and highlights the importance of a proper analysis of both fabrication parameters and sample preparation before any conclusion on reactive selectivity at the interface can be reached.

Krasnoselsky S.s. - One of the best experts on this subject based on the ideXlab platform.