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Masaru Hori - One of the best experts on this subject based on the ideXlab platform.
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differential data on the responsiveness of multiple cell types to cell death induced by non thermal atmospheric Pressure Plasma activated solutions
Data in Brief, 2021Co-Authors: Ko Eto, Hiromasa Tanaka, Chiaki Ishinada, Takuya Suemoto, Keiichiro Hyakutake, Masaru HoriAbstract:A discovery that cells die of a novel and distinctive process, along with some characteristic events, such as cellular shrinkage and Programmed cell death 4 disappearance, has been done by using non-thermal atmospheric Pressure Plasma-activated solutions [1]. Data on the responsiveness of multiple cell types to the induction of cellular shrinkage and cell death and the loss of Programmed cell death 4 by exposure to the non-thermal atmospheric Pressure Plasma-activated solutions were collected. Human neuroblastoma SH-SY5Y cells, murine myoblast C2C12 cells, and murine embryonic fibroblasts were cultured for various periods in each of the non-thermal atmospheric Pressure Plasma-activated solutions and then examined by light field microscopic observation for their effects on cell morphology, by Trypan blue dye exclusion assay for those on cell death, and by Western blotting for those on Programmed cell death 4 disappearance. The data clarified some differences in the responsiveness to the induction of cellular shrinkage, cell death, and Pdcd4 disappearance by all the non-thermal atmospheric Pressure Plasma-activated solutions among the cells.
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non thermal atmospheric Pressure Plasma activates lactate in ringer s solution for anti tumor effects
Scientific Reports, 2016Co-Authors: Hiromasa Tanaka, Kae Nakamura, Hiroaki Kajiyama, Masaaki Mizuno, Fumitaka Kikkawa, Fumi Utsumi, Kenji Ishikawa, Keigo Takeda, Masaru HoriAbstract:Non-thermal atmospheric Pressure Plasma is a novel approach for wound healing, blood coagulation, and cancer therapy. A recent discovery in the field of Plasma medicine is that non-thermal atmospheric Pressure Plasma not only directly but also indirectly affects cells via Plasma-treated liquids. This discovery has led to the use of non-thermal atmospheric Pressure Plasma as a novel chemotherapy. We refer to these Plasma-treated liquids as Plasma-activated liquids. We chose Ringer’s solutions to produce Plasma-activated liquids for clinical applications. In vitro and in vivo experiments demonstrated that Plasma-activated Ringer’s lactate solution has anti-tumor effects, but of the four components in Ringer’s lactate solution, only lactate exhibited anti-tumor effects through activation by non-thermal Plasma. Nuclear magnetic resonance analyses indicate that Plasma irradiation generates acetyl and pyruvic acid-like groups in Ringer’s lactate solution. Overall, these results suggest that Plasma-activated Ringer’s lactate solution is promising for chemotherapy.
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synthesis of calcium oxalate crystals in culture medium irradiated with non equilibrium atmospheric Pressure Plasma
Applied Physics Express, 2016Co-Authors: Naoyuki Kurake, Kae Nakamura, Hiroaki Kajiyama, Hiromasa Tanaka, Masaaki Mizuno, Fumitaka Kikkawa, Kenji Ishikawa, Yoko Yamanishi, Masaru HoriAbstract:Octahedral particulates several tens of microns in size were synthesized in a culture medium irradiated through contact with a plume of non-equilibrium atmospheric-Pressure Plasma (NEAPP). The particulates were identified in the crystalline phase as calcium oxalate dihydrate (COD). The original medium contained constituents such as NaCl, d-glucose, CaCl2, and NaHCO3 but not oxalate or oxalic acid. The oxalate was clearly synthesized and crystallized in the medium as thermodynamically unstable COD crystals after the NEAPP irradiation.
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cancer therapy using non thermal atmospheric Pressure Plasma with ultra high electron density
Physics of Plasmas, 2015Co-Authors: Hiromasa Tanaka, Tetsuo Adachi, Masaaki Mizuno, Shinya Toyokuni, Shoichi Maruyama, Yasuhiro Kodera, Hiroko Terasaki, Masashi Kato, Fumitaka Kikkawa, Masaru HoriAbstract:Cancer therapy using non-thermal atmospheric Pressure Plasma is a big challenge in Plasma medicine. Reactive species generated from Plasma are key factors for treating cancer cells, and thus, non-thermal atmospheric Pressure Plasma with high electron density has been developed and applied for cancer treatment. Various cancer cell lines have been treated with Plasma, and non-thermal atmospheric Plasma clearly has anti-tumor effects. Recent innovative studies suggest that Plasma can both directly and indirectly affect cells and tissues, and this observation has widened the range of applications. Thus, cancer therapy using non-thermal atmospheric Pressure Plasma is promising. Animal experiments and understanding the mode of action are essential for clinical application in the future. A new academic field that combines Plasma science, the biology of free radicals, and systems biology will be established.
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selective cytotoxicity of indirect nonequilibrium atmospheric Pressure Plasma against ovarian clear cell carcinoma
SpringerPlus, 2014Co-Authors: Fumi Utsumi, Kae Nakamura, Hiroaki Kajiyama, Hiromasa Tanaka, Masaru Hori, Fumitaka KikkawaAbstract:Ovarian clear cell carcinoma (CCC) is a histological type of epithelial ovarian cancer that is less responsive to chemotherapy and associated with a poorer prognosis than serous and endometrioid carcinoma. Non-thermal atmospheric Pressure Plasma which produces reactive species has recently led to an explosion of research in Plasma medicine. Plasma treatment can be applied to cancer treatment to induce apoptosis and tumor growth arrest. Furthermore, recent studies have shown that a medium exposed to Plasma also has an anti-proliferative effect against cancer in the absence of direct exposure to Plasma. In this study, we confirmed whether this indirect Plasma has an anti-tumor effect against CCC, and investigated whether this efficacy is selective for cancer cells. Non-thermal atmospheric Pressure Plasma induced apoptosis in CCC cells, while human peritoneal mesothelial cells remained viable. Non-thermal atmospheric Pressure Plasma exhibits selective cytotoxicity against CCC cells which are resistant to chemotherapy.
Hiromasa Tanaka - One of the best experts on this subject based on the ideXlab platform.
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differential data on the responsiveness of multiple cell types to cell death induced by non thermal atmospheric Pressure Plasma activated solutions
Data in Brief, 2021Co-Authors: Ko Eto, Hiromasa Tanaka, Chiaki Ishinada, Takuya Suemoto, Keiichiro Hyakutake, Masaru HoriAbstract:A discovery that cells die of a novel and distinctive process, along with some characteristic events, such as cellular shrinkage and Programmed cell death 4 disappearance, has been done by using non-thermal atmospheric Pressure Plasma-activated solutions [1]. Data on the responsiveness of multiple cell types to the induction of cellular shrinkage and cell death and the loss of Programmed cell death 4 by exposure to the non-thermal atmospheric Pressure Plasma-activated solutions were collected. Human neuroblastoma SH-SY5Y cells, murine myoblast C2C12 cells, and murine embryonic fibroblasts were cultured for various periods in each of the non-thermal atmospheric Pressure Plasma-activated solutions and then examined by light field microscopic observation for their effects on cell morphology, by Trypan blue dye exclusion assay for those on cell death, and by Western blotting for those on Programmed cell death 4 disappearance. The data clarified some differences in the responsiveness to the induction of cellular shrinkage, cell death, and Pdcd4 disappearance by all the non-thermal atmospheric Pressure Plasma-activated solutions among the cells.
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non thermal atmospheric Pressure Plasma activates lactate in ringer s solution for anti tumor effects
Scientific Reports, 2016Co-Authors: Hiromasa Tanaka, Kae Nakamura, Hiroaki Kajiyama, Masaaki Mizuno, Fumitaka Kikkawa, Fumi Utsumi, Kenji Ishikawa, Keigo Takeda, Masaru HoriAbstract:Non-thermal atmospheric Pressure Plasma is a novel approach for wound healing, blood coagulation, and cancer therapy. A recent discovery in the field of Plasma medicine is that non-thermal atmospheric Pressure Plasma not only directly but also indirectly affects cells via Plasma-treated liquids. This discovery has led to the use of non-thermal atmospheric Pressure Plasma as a novel chemotherapy. We refer to these Plasma-treated liquids as Plasma-activated liquids. We chose Ringer’s solutions to produce Plasma-activated liquids for clinical applications. In vitro and in vivo experiments demonstrated that Plasma-activated Ringer’s lactate solution has anti-tumor effects, but of the four components in Ringer’s lactate solution, only lactate exhibited anti-tumor effects through activation by non-thermal Plasma. Nuclear magnetic resonance analyses indicate that Plasma irradiation generates acetyl and pyruvic acid-like groups in Ringer’s lactate solution. Overall, these results suggest that Plasma-activated Ringer’s lactate solution is promising for chemotherapy.
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synthesis of calcium oxalate crystals in culture medium irradiated with non equilibrium atmospheric Pressure Plasma
Applied Physics Express, 2016Co-Authors: Naoyuki Kurake, Kae Nakamura, Hiroaki Kajiyama, Hiromasa Tanaka, Masaaki Mizuno, Fumitaka Kikkawa, Kenji Ishikawa, Yoko Yamanishi, Masaru HoriAbstract:Octahedral particulates several tens of microns in size were synthesized in a culture medium irradiated through contact with a plume of non-equilibrium atmospheric-Pressure Plasma (NEAPP). The particulates were identified in the crystalline phase as calcium oxalate dihydrate (COD). The original medium contained constituents such as NaCl, d-glucose, CaCl2, and NaHCO3 but not oxalate or oxalic acid. The oxalate was clearly synthesized and crystallized in the medium as thermodynamically unstable COD crystals after the NEAPP irradiation.
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cancer therapy using non thermal atmospheric Pressure Plasma with ultra high electron density
Physics of Plasmas, 2015Co-Authors: Hiromasa Tanaka, Tetsuo Adachi, Masaaki Mizuno, Shinya Toyokuni, Shoichi Maruyama, Yasuhiro Kodera, Hiroko Terasaki, Masashi Kato, Fumitaka Kikkawa, Masaru HoriAbstract:Cancer therapy using non-thermal atmospheric Pressure Plasma is a big challenge in Plasma medicine. Reactive species generated from Plasma are key factors for treating cancer cells, and thus, non-thermal atmospheric Pressure Plasma with high electron density has been developed and applied for cancer treatment. Various cancer cell lines have been treated with Plasma, and non-thermal atmospheric Plasma clearly has anti-tumor effects. Recent innovative studies suggest that Plasma can both directly and indirectly affect cells and tissues, and this observation has widened the range of applications. Thus, cancer therapy using non-thermal atmospheric Pressure Plasma is promising. Animal experiments and understanding the mode of action are essential for clinical application in the future. A new academic field that combines Plasma science, the biology of free radicals, and systems biology will be established.
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selective cytotoxicity of indirect nonequilibrium atmospheric Pressure Plasma against ovarian clear cell carcinoma
SpringerPlus, 2014Co-Authors: Fumi Utsumi, Kae Nakamura, Hiroaki Kajiyama, Hiromasa Tanaka, Masaru Hori, Fumitaka KikkawaAbstract:Ovarian clear cell carcinoma (CCC) is a histological type of epithelial ovarian cancer that is less responsive to chemotherapy and associated with a poorer prognosis than serous and endometrioid carcinoma. Non-thermal atmospheric Pressure Plasma which produces reactive species has recently led to an explosion of research in Plasma medicine. Plasma treatment can be applied to cancer treatment to induce apoptosis and tumor growth arrest. Furthermore, recent studies have shown that a medium exposed to Plasma also has an anti-proliferative effect against cancer in the absence of direct exposure to Plasma. In this study, we confirmed whether this indirect Plasma has an anti-tumor effect against CCC, and investigated whether this efficacy is selective for cancer cells. Non-thermal atmospheric Pressure Plasma induced apoptosis in CCC cells, while human peritoneal mesothelial cells remained viable. Non-thermal atmospheric Pressure Plasma exhibits selective cytotoxicity against CCC cells which are resistant to chemotherapy.
Klaus-dieter Weltmann - One of the best experts on this subject based on the ideXlab platform.
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atmospheric Pressure Plasma jets an overview of devices and new directions
Plasma Sources Science and Technology, 2015Co-Authors: Jorn Winter, Ronny Brandenburg, Klaus-dieter WeltmannAbstract:Atmospheric Pressure Plasma jets have a long history of more than 50 years. During this time their design and Plasma generation mechanism has been developed and adapted to various fields of applications. This review aims at giving an overview of jet devices by starting with a brief history of their development. This is followed by an overview of commonly used terms and definitions as well as a survey of different classification schemes (e.g. geometry, excition frequency or specific energy input) described in literature. A selective update of new designs and novel research achievments on atmospheric Pressure Plasma jets published in 2012 or later shows the impressive variety and rapid development of the field. Finally, a brief outlook on the future trends and directions is given.
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Non-thermal atmospheric-Pressure Plasma possible application in wound healing
Biomolecules and Therapeutics, 2014Co-Authors: Beate Haertel, Thomas Von Woedtke, Klaus-dieter Weltmann, Ulrike LindequistAbstract:Non-thermal atmospheric-Pressure Plasma, also named cold Plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with Plasma from blood; it is not biological in nature. Non-thermal atmospheric-Pressure Plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes Plasma effects on wound healing. Non-thermal atmospheric-Pressure Plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of Plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of Plasma. The outcome of first clinical trials regarding wound healing is pointed out.
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Controlling the NO production of an atmospheric Pressure Plasma jet
Journal of Physics D: Applied Physics, 2012Co-Authors: A V Pipa, Rdiger Foest, Stephan Reuter, Klaus-dieter WeltmannAbstract:The production of NO radicals by an atmospheric Pressure Plasma jet has been investigated by means of absorption spectroscopy in the mid-infrared region (IR) and optical emission spectroscopy (OES) in the ultraviolet (UV) part of the spectrum. The Plasma jet investigated here operates in argon with air admixtures up to 1%. The study shows that OES can be used to characterize the relative NO production at small air admixtures. The Production of NO radicals can be controlled by variation of air admixture. Important to note—especially for operation in ambient conditions—is that a small addition of water vapour strongly affects the production of NO radicals especially at higher air admixtures (greater than 0.2%).
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high rate etching of polymers by means of an atmospheric Pressure Plasma jet
Plasma Processes and Polymers, 2011Co-Authors: Katja Fricke, Thomas Von Woedtke, H Steffen, Karsten Schroder, Klaus-dieter WeltmannAbstract:The impact of atmospheric Pressure Plasma on surfaces, in particular its potential application of modification and decontamination of different materials has been intensively investigated. In this study, the etching capability of an atmospheric Pressure Plasma jet is shown. A variety of polymers [e.g., polyethylene and poly (ether ether ketone)] was exposed to pure argon and argon/oxygen Plasma. The influence of the oxygen admixture (up to 1%) and of the jet-nozzle to substrate distance on the etch rate of chemically different polymers was explored. Particular attention was applied on the feasible use of atmospheric Pressure Plasma on biofilm removal. For that reason a theory was postulated with each polymer representing a model compound of bacterial cells. The etch rates were obtained by determination of the mass loss and etch profiles after Plasma exposure. The experiments showed that reactive oxygen species play an important role in the polymer removal which results in etch rates of 50 up to 300 nm · s ―1 depending on the polymeric material. These high etch rates imply that non-thermal atmospheric Plasma jets could be used for removal of organic material including micro-organisms from surfaces.
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In vitro activity of atmospheric Pressure Plasma jet (APPJ) Plasma against clinical isolates of Demodex folliculorum
IEEE Transactions on Plasma Science, 2010Co-Authors: Georg Daeschlein, Thomas Von Woedtke, Maria Niggemeier, Andreas Arnold, Eckhard Kindel, Klaus-dieter Weltmann, Sebastian Scholz, Michael JüngerAbstract:Rosacea is a frequent and often disfiguring and chronic dermatologic disease mainly of the midface causing central facial erythema, teleangiectasia, papules, and pustules. In the context of complex pathogenicity, Demodex folliculorum plays an important role showing significant density in the skin of patients with papulopustular rosacea. Rosacea belongs to the hard-to-heal diseases, and new approaches for treatment are strongly required. As atmospheric low-temperature Plasma [atmospheric Pressure Plasma jet (APPJ)] proved high efficacy against bacterial and fungal pathogens, we tested its potency as nonantibiotic-based method to inactivate Demodex folliculorum. We isolated five parasites of Demodex folliculorum from a 54-year-old patient suffering from chronic pustulous rosacea and irradiated the living parasites by APPJ ex vivo. The APPJ Plasma killed Demodex folliculorum after an exposure time of 2 resp. 60 s. Low-temperature atmospheric Pressure Plasma seems suitable for the treatment of dermatologic and veterinarian diseases caused by Demodex spp.
R F Hicks - One of the best experts on this subject based on the ideXlab platform.
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deposition of silicon dioxide films with a non equilibrium atmospheric Pressure Plasma jet
Plasma Sources Science and Technology, 2001Co-Authors: S E Babayan, Gary S Selwyn, J.-y. Jeong, A Schutze, M Moravej, R F HicksAbstract:Silicon dioxide films were grown using an atmospheric-Pressure Plasma jet that was produced by flowing oxygen and helium between two coaxial metal electrodes that were driven by 13.56 MHz radio frequency power. The Plasma exiting from between the electrodes was mixed with tetraethoxysilane (TEOS), and directed onto a silicon substrate held at 115-350 °C. Silicon dioxide films were deposited at rates ranging from 20±2 to 300±25 nm min-1. The deposition rate increased with decreasing temperature and increasing TEOS Pressure, oxygen Pressure and RF power. For the latter two variables, the rate increased as follows: Rd∝P0.3O2(RF)1.4. Films grown at 115 °C were porous and contained adsorbed hydroxyl groups, whereas films grown at 350 °C were smooth, dense and free of impurities. These results suggest that the mechanism in the atmospheric Pressure Plasma is the same as that in low-Pressure Plasmas.
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an atmospheric Pressure Plasma source
Applied Physics Letters, 2000Co-Authors: Jaeyoung Park, J.-y. Jeong, I Henins, H W Herrmann, G S Selwyn, R F Hicks, D Shim, C S ChangAbstract:An atmospheric Pressure Plasma source operated by radio frequency power has been developed. This source produces a unique discharge that is volumetric and homogeneous at atmospheric Pressure with a gas temperature below 300 °C. It also produces a large quantity of oxygen atoms, ∼5×1015 cm−3, which has important value for materials applications. A theoretical model shows electron densities of 0.2–2×1011 cm−3 and characteristic electron energies of 2–4 eV for helium discharges at a power level of 3–30 W cm−3.
Hiroyuki Kano - One of the best experts on this subject based on the ideXlab platform.
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Plasma Medical Science for Cancer Therapy: Toward Cancer Therapy Using Nonthermal Atmospheric Pressure Plasma
IEEE Transactions on Plasma Science, 2014Co-Authors: Hiromasa Tanaka, Kae Nakamura, Hiroaki Kajiyama, Hiroyuki Kano, Masaaki Mizuno, Fumi Utsumi, Kenji Ishikawa, Keigo Takeda, Yasumasa Okazaki, Shinnya ToyokuniAbstract:We have been developing novel ultrahigh density atmospheric Pressure Plasma sources and succeeded in the selective killing ovarian cancer cells against normal ones. Furthermore, we have found out the Plasma-activated medium (PAM) also killed glioblastoma brain tumor cells selectively against normal ones and the chemical products in the PAM have long lifetime healing effects. To clarify the mechanism, interactions of Plasma with the organism and the medium where the organism belongs were investigated on the viewpoint of intracellular molecular mechanism.
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laser scattering diagnosis of a 60 hz non equilibrium atmospheric Pressure Plasma jet
Applied Physics Express, 2011Co-Authors: Fengdong Jia, Hiroyuki Kano, Kenji Ishikawa, Keigo Takeda, Naoya Sumi, Hirotoshi Inui, Jagath S Kularatne, Hiroki Kondo, Makoto Sekine, Akihiro KonoAbstract:A non-equilibrium atmospheric Pressure Plasma jet excited by 60-Hz ac power was diagnosed by laser Thomson and laser Raman scattering. We obtained the spatial distributions of the electron density, electron temperature, and gas temperature. The results show that the Plasma can generate an electron density of up to 1021 m-3, an electron temperature of approximately 1 eV, and a gas temperature as low as approximately 700 K, indicating that the Plasma is in the non-equilibrium state. The laser scattering diagnostic method and the obtained data are useful in the application of the non-equilibrium atmospheric Pressure Plasma jet.
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rapid inactivation of penicillium digitatum spores using high density nonequilibrium atmospheric Pressure Plasma
Applied Physics Letters, 2010Co-Authors: Sachiko Iseki, Akiyoshi Aomatsu, Yasuhiro Higashijima, Hiroyuki Kano, Takayuki Ohta, Masaru HoriAbstract:A promising, environmentally safe method for inactivating fungal spores of Penicillium digitatum, a difficult-to-inactivate food spoilage microorganism, was developed using a high-density nonequilibrium atmospheric Pressure Plasma (NEAPP). The NEAPP employing Ar gas had a high electron density on the order of 1015 cm−3. The spores were successfully and rapidly inactivated using the NEAPP, with a decimal reduction time in spores (D value) of 1.7 min. The contributions of ozone and UV radiation on the inactivation of the spores were evaluated and concluded to be not dominant, which was fundamentally different from the conventional sterilizations.
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etching process of silicon dioxide with nonequilibrium atmospheric Pressure Plasma
Journal of Applied Physics, 2005Co-Authors: Koji Yamakawa, Masaru Hori, Toshio Goto, Shoji Den, Toshirou Katagiri, Hiroyuki KanoAbstract:An ultrahigh etch rate (14μm∕min) of SiO2 and a high selectivity of SiO2∕Si over 200 were achieved using a microwave-excited nonequilibrium atmospheric Pressure Plasma source employing He, NF3, and H2O gases, which have been developed for application to microelectromechanical systems and other bionanotechnology fields. In order to clarify the etching mechanism, two diagnostic methods have been performed: (1) imaging of Plasma emission with an intensified charge-coupled device camera, and (2) absorption measurements using Fourier transform infrared spectroscopy. The etching characteristics are discussed in relation to the spatial distributions of the species involved. The etch rate depended considerably on the distance between the Plasma and the substrate. Some radicals generated from the feed gases reached the substrate directly, while other radicals recombined into different species, which reached the substrate. An abundance of HF molecules were produced through a reaction between radicals generated by t...
