The Experts below are selected from a list of 2214 Experts worldwide ranked by ideXlab platform
Mst. Mansura Khan - One of the best experts on this subject based on the ideXlab platform.
-
Studying the effects of Profenofos, an endocrine disruptor, on organogenesis of zebrafish
Environmental Science and Pollution Research, 2021Co-Authors: Zakia Sultana, Mst. Mansura Khan, Golam Mohammod Mostakim, Md. Moniruzzaman, Md. Khalilur Rahman, Md. Shahjahan, M. Sadiqul IslamAbstract:Profenofos is an endocrine-disrupting chemical that can enter into the aquatic ecosystem either through surface runoff or through percolation of a toxicant from the soil. In order to clarify the effect of Profenofos on the developmental stages of zebrafish, the embryos were treated with serial dilutions of Profenofos (0%, 10%, 25%, and 50% of LC_50). Embryos were treated with Profenofos for 7 days or until hatching. The toxic endpoints assessed include hatching time, survival, malformation, and heartbeats of the embryos. In a 96-h test on zebrafish embryos, the LC_50 of Profenofos was 0.057 mg/L. Profenofos considerably lowered survival, increased abnormalities at different ontogenetic stages, and developed malformations of different organs in a concentration-dependent fashion. The identified developmental malformations were fluid accumulation, impaired jaw, short tail, ruptured pectoral and caudal fin, curved body, thin yolk sac tube, and deformed heart. The way of looping arrangement of the heart at the early stage of embryos was significantly influenced by the higher concentration of Profenofos. Heartbeat is also reduced significantly in a concentration-dependent fashion. The results show that the zebrafish are susceptible to Profenofos even at lower concentrations in the initial stage. Therefore, when used in agricultural areas adjacent to the aquatic environment, endocrine-disrupting chemicals should be used in an appropriate manner.
-
aberrations of the peripheral erythrocytes and its recovery patterns in a freshwater teleost silver barb exposed to Profenofos
Environmental Pollution, 2018Co-Authors: Mst. Mansura Khan, M. Moniruzzaman, Golam Mohammod Mostakim, Mohammad Sadequr Rahman Khan, Md. Khalilur Rahman, Sadiqul M IslamAbstract:The present experiment was conducted to explicate the genotoxic effects of Profenofos, an organophosphate insecticide, on the erythrocytes of silver barb (Barbonymus gonionotus). Silver barb were exposed to a solution of 10% and 50% of lethal concentrations (LC50) of Profenofos as sub-lethal concentrations at different days (1, 7, 15, and 30 d), along with a control (0% Profenofos). Subsequent recovery patterns were assessed allowing the fish exposed to Profenofos free water for the same period that they were exposed to Profenofos. Our results revealed that with the progression of time and concentration, fish exposed to Profenofos showed significantly (p < .05) higher level of erythrocytic nuclear abnormalities (ENA) such as micronuclei, bi-nuclei, degenerated nuclei, notched nuclei, nuclear bridge and nuclear buds, as well as erythrocytic cellular abnormalities (ECA) such as echinocytic, elongated, fusion, spindle, tear-drop and twin shaped cells. After exposure, the silver barb recovered spontaneously, and the abnormal erythrocytic parameters were normalized with a concentration- and duration-dependent fashion. Therefore, these abnormalities and their recovery can be used to assess the toxic levels of pesticides on aquatic organisms. There is great potential to use this technique as in vivo to predict susceptibility of aquatic animals to environmental pollution.
-
Aberrations of the peripheral erythrocytes and its recovery patterns in a freshwater teleost, silver barb exposed to Profenofos.
Environmental pollution (Barking Essex : 1987), 2017Co-Authors: Mst. Mansura Khan, M. Moniruzzaman, Golam Mohammod Mostakim, Mohammad Sadequr Rahman Khan, Khalilur Rahman, M. Sadiqul IslamAbstract:The present experiment was conducted to explicate the genotoxic effects of Profenofos, an organophosphate insecticide, on the erythrocytes of silver barb (Barbonymus gonionotus). Silver barb were exposed to a solution of 10% and 50% of lethal concentrations (LC50) of Profenofos as sub-lethal concentrations at different days (1, 7, 15, and 30 d), along with a control (0% Profenofos). Subsequent recovery patterns were assessed allowing the fish exposed to Profenofos free water for the same period that they were exposed to Profenofos. Our results revealed that with the progression of time and concentration, fish exposed to Profenofos showed significantly (p
Sanjay D. Sawant - One of the best experts on this subject based on the ideXlab platform.
-
Biodegradation of Profenofos by Bacillus subtilis Isolated from Grapevines (Vitis vinifera)
Journal of agricultural and food chemistry, 2013Co-Authors: Varsha P. Salunkhe, Indu S. Sawant, Kaushik Banerjee, Yogita R. Rajguru, Pallavi N. Wadkar, Dasharath P. Oulkar, Dattatraya G. Naik, Sanjay D. SawantAbstract:The biodegradation of Profenofos, an organophosphorus insecticide, by four Bacillus subtilis strains, namely, DR-39, CS-126, TL-171, and TS-204, isolated from grapevines or grape rhizosphere was studied in liquid culture, on grape berries, and in vineyard soil. Each of the four B. subtilis strains enhanced the degradation of Profenofos in all three matrices. Degradation rate constants were best obtained by first + first-order kinetics module. In nutrient broth spiked with 5 μg/mL Profenofos, inoculation with B. subtilis strains DR-39, CS-126, TL-171, and TS-204 reduced the half-life (DT50) of Profenofos to 4.03, 3.57, 2.87, and 2.53 days, respectively, from the DT50 = 12.90 days observed in the uninoculated control. In Thompson Seedless grapes sprayed with Profenofos at a field dose of 1250 mL ai/ha, the DT50 values were 1.07, 1.00, 2.13, and 2.20 days in grapes inoculated with B. subtilis strains DR-39, CS-126, TL-171, and TS-204, respectively, as compared to 2.20 days in uninoculated grapes. These four B. subtilis strains also enhanced the degradation of Profenofos in autoclaved soil (DT50 = 5.93, 7.47, 6.00, and 4.37 days) and in nonautoclaved soil (DT50 = 0.87, 2.00, 2.07, and 2.43 days) amended with 5 μg/g Profenofos from the half-lives of 17.37 and 14.37 days in respective uninoculated soils. Growth dynamic studies indicated that all four B. subtilis strains were able to establish and proliferate on berries and soil equally well in the presence or absence of Profenofos. Degradation product 4-bromo-2-chlorophenol was identified by GC-MS. Strain DR-39 was most effective in the natural environments of grape and soil.
-
biodegradation of Profenofos by bacillus subtilis isolated from grapevines vitis vinifera
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Varsha P. Salunkhe, Indu S. Sawant, Kaushik Banerjee, Yogita R. Rajguru, Pallavi N. Wadkar, Dasharath P. Oulkar, Dattatraya G. Naik, Sanjay D. SawantAbstract:The biodegradation of Profenofos, an organophosphorus insecticide, by four Bacillus subtilis strains, namely, DR-39, CS-126, TL-171, and TS-204, isolated from grapevines or grape rhizosphere was studied in liquid culture, on grape berries, and in vineyard soil. Each of the four B. subtilis strains enhanced the degradation of Profenofos in all three matrices. Degradation rate constants were best obtained by first + first-order kinetics module. In nutrient broth spiked with 5 μg/mL Profenofos, inoculation with B. subtilis strains DR-39, CS-126, TL-171, and TS-204 reduced the half-life (DT50) of Profenofos to 4.03, 3.57, 2.87, and 2.53 days, respectively, from the DT50 = 12.90 days observed in the uninoculated control. In Thompson Seedless grapes sprayed with Profenofos at a field dose of 1250 mL ai/ha, the DT50 values were 1.07, 1.00, 2.13, and 2.20 days in grapes inoculated with B. subtilis strains DR-39, CS-126, TL-171, and TS-204, respectively, as compared to 2.20 days in uninoculated grapes. These four ...
M. Sadiqul Islam - One of the best experts on this subject based on the ideXlab platform.
-
Studying the effects of Profenofos, an endocrine disruptor, on organogenesis of zebrafish
Environmental Science and Pollution Research, 2021Co-Authors: Zakia Sultana, Mst. Mansura Khan, Golam Mohammod Mostakim, Md. Moniruzzaman, Md. Khalilur Rahman, Md. Shahjahan, M. Sadiqul IslamAbstract:Profenofos is an endocrine-disrupting chemical that can enter into the aquatic ecosystem either through surface runoff or through percolation of a toxicant from the soil. In order to clarify the effect of Profenofos on the developmental stages of zebrafish, the embryos were treated with serial dilutions of Profenofos (0%, 10%, 25%, and 50% of LC_50). Embryos were treated with Profenofos for 7 days or until hatching. The toxic endpoints assessed include hatching time, survival, malformation, and heartbeats of the embryos. In a 96-h test on zebrafish embryos, the LC_50 of Profenofos was 0.057 mg/L. Profenofos considerably lowered survival, increased abnormalities at different ontogenetic stages, and developed malformations of different organs in a concentration-dependent fashion. The identified developmental malformations were fluid accumulation, impaired jaw, short tail, ruptured pectoral and caudal fin, curved body, thin yolk sac tube, and deformed heart. The way of looping arrangement of the heart at the early stage of embryos was significantly influenced by the higher concentration of Profenofos. Heartbeat is also reduced significantly in a concentration-dependent fashion. The results show that the zebrafish are susceptible to Profenofos even at lower concentrations in the initial stage. Therefore, when used in agricultural areas adjacent to the aquatic environment, endocrine-disrupting chemicals should be used in an appropriate manner.
-
Aberrations of the peripheral erythrocytes and its recovery patterns in a freshwater teleost, silver barb exposed to Profenofos.
Environmental pollution (Barking Essex : 1987), 2017Co-Authors: Mst. Mansura Khan, M. Moniruzzaman, Golam Mohammod Mostakim, Mohammad Sadequr Rahman Khan, Khalilur Rahman, M. Sadiqul IslamAbstract:The present experiment was conducted to explicate the genotoxic effects of Profenofos, an organophosphate insecticide, on the erythrocytes of silver barb (Barbonymus gonionotus). Silver barb were exposed to a solution of 10% and 50% of lethal concentrations (LC50) of Profenofos as sub-lethal concentrations at different days (1, 7, 15, and 30 d), along with a control (0% Profenofos). Subsequent recovery patterns were assessed allowing the fish exposed to Profenofos free water for the same period that they were exposed to Profenofos. Our results revealed that with the progression of time and concentration, fish exposed to Profenofos showed significantly (p
Rajesh Pamanji - One of the best experts on this subject based on the ideXlab platform.
-
Profenofos induced biochemical alterations and in silico modelling of hatching enzyme, ZHE1 in zebrafish (Danio rerio) embryos.
Environmental toxicology and pharmacology, 2016Co-Authors: Rajesh Pamanji, Bomma Yashwanth, Janapala Venkateswara RaoAbstract:The current study was aimed to investigate the oxidative stress response in zebrafish embryos exposed to sub-lethal (LC10) and lethal (LC50) concentrations of Profenofos for 96-h and in silico modelling of zebrafish hatching enzyme, ZHE1 to explain the delayed hatching. Embryos exposed to Profenofos under semi-static conditions significantly diminished glutathione (GSH), superoxide dismutase (SOD) and glutathione reductase (GR) levels, but increased the activities of catalase (CAT) and glutathione S-transferase (GST) concomitantly with marked elevation in malondialdehyde (MDA) content in whole-body homogenate of the treated groups compared with control. In addition, stress protein Hsp70 expression and DNA damage were significantly increased in a concentration- dependent manner compared with controls. From the computational docking studies of ZHE1 with Profenofos revealed that Profenofos is binding to three amino acids, histidine 99, histidine 109 and arginine 182 at the active site of the enzyme through hydrogen bonding which may lead to inhibition of hatching.
-
Toxicity effects of Profenofos on embryonic and larval development of Zebrafish (Danio rerio).
Environmental toxicology and pharmacology, 2015Co-Authors: Rajesh Pamanji, Bomma Yashwanth, M. S. Bethu, S. Leelavathi, K. Ravinder, J. Venkateswara RaoAbstract:Abstract The aim of the present study was to evaluate the developmental toxicity of Profenofos to early developing Zebrafish ( Danio rerio ) embryos (4 h post fertilization) in a static system at 1.0 to 2.25 mg/L. Median lethal concentrations (LC 50 ) of Profenofos at 24-h, 48-h, 72-h and 96-h were determined as 2.04, 1.58, 1.57 and 1.56 mg/L, respectively. The hatching of embryos were recorded at every 12 h interval and the median hatching time (HT 50 ) was also calculated for each concentration. In a separate set of experiments, 96-h LC 10 (0.74 mg/L) and LC 50 (1.56 mg/L) concentrations were used to assess the developmental toxicity in relation to behavior, morphology, and interactions with the targeted enzyme acetylcholinesterase. Live video-microscopy revealed that the Profenofos exposed embryos exhibited an abnormal development, skeletal defects and altered heart morphology in a concentration-dependent manner, which leads to alterations in the swimming behavior of hatchlings at 144-h, which indicate that developing zebrafish are sensitive to Profenofos.
Golam Mohammod Mostakim - One of the best experts on this subject based on the ideXlab platform.
-
Studying the effects of Profenofos, an endocrine disruptor, on organogenesis of zebrafish
Environmental Science and Pollution Research, 2021Co-Authors: Zakia Sultana, Mst. Mansura Khan, Golam Mohammod Mostakim, Md. Moniruzzaman, Md. Khalilur Rahman, Md. Shahjahan, M. Sadiqul IslamAbstract:Profenofos is an endocrine-disrupting chemical that can enter into the aquatic ecosystem either through surface runoff or through percolation of a toxicant from the soil. In order to clarify the effect of Profenofos on the developmental stages of zebrafish, the embryos were treated with serial dilutions of Profenofos (0%, 10%, 25%, and 50% of LC_50). Embryos were treated with Profenofos for 7 days or until hatching. The toxic endpoints assessed include hatching time, survival, malformation, and heartbeats of the embryos. In a 96-h test on zebrafish embryos, the LC_50 of Profenofos was 0.057 mg/L. Profenofos considerably lowered survival, increased abnormalities at different ontogenetic stages, and developed malformations of different organs in a concentration-dependent fashion. The identified developmental malformations were fluid accumulation, impaired jaw, short tail, ruptured pectoral and caudal fin, curved body, thin yolk sac tube, and deformed heart. The way of looping arrangement of the heart at the early stage of embryos was significantly influenced by the higher concentration of Profenofos. Heartbeat is also reduced significantly in a concentration-dependent fashion. The results show that the zebrafish are susceptible to Profenofos even at lower concentrations in the initial stage. Therefore, when used in agricultural areas adjacent to the aquatic environment, endocrine-disrupting chemicals should be used in an appropriate manner.
-
aberrations of the peripheral erythrocytes and its recovery patterns in a freshwater teleost silver barb exposed to Profenofos
Environmental Pollution, 2018Co-Authors: Mst. Mansura Khan, M. Moniruzzaman, Golam Mohammod Mostakim, Mohammad Sadequr Rahman Khan, Md. Khalilur Rahman, Sadiqul M IslamAbstract:The present experiment was conducted to explicate the genotoxic effects of Profenofos, an organophosphate insecticide, on the erythrocytes of silver barb (Barbonymus gonionotus). Silver barb were exposed to a solution of 10% and 50% of lethal concentrations (LC50) of Profenofos as sub-lethal concentrations at different days (1, 7, 15, and 30 d), along with a control (0% Profenofos). Subsequent recovery patterns were assessed allowing the fish exposed to Profenofos free water for the same period that they were exposed to Profenofos. Our results revealed that with the progression of time and concentration, fish exposed to Profenofos showed significantly (p < .05) higher level of erythrocytic nuclear abnormalities (ENA) such as micronuclei, bi-nuclei, degenerated nuclei, notched nuclei, nuclear bridge and nuclear buds, as well as erythrocytic cellular abnormalities (ECA) such as echinocytic, elongated, fusion, spindle, tear-drop and twin shaped cells. After exposure, the silver barb recovered spontaneously, and the abnormal erythrocytic parameters were normalized with a concentration- and duration-dependent fashion. Therefore, these abnormalities and their recovery can be used to assess the toxic levels of pesticides on aquatic organisms. There is great potential to use this technique as in vivo to predict susceptibility of aquatic animals to environmental pollution.
-
Aberrations of the peripheral erythrocytes and its recovery patterns in a freshwater teleost, silver barb exposed to Profenofos.
Environmental pollution (Barking Essex : 1987), 2017Co-Authors: Mst. Mansura Khan, M. Moniruzzaman, Golam Mohammod Mostakim, Mohammad Sadequr Rahman Khan, Khalilur Rahman, M. Sadiqul IslamAbstract:The present experiment was conducted to explicate the genotoxic effects of Profenofos, an organophosphate insecticide, on the erythrocytes of silver barb (Barbonymus gonionotus). Silver barb were exposed to a solution of 10% and 50% of lethal concentrations (LC50) of Profenofos as sub-lethal concentrations at different days (1, 7, 15, and 30 d), along with a control (0% Profenofos). Subsequent recovery patterns were assessed allowing the fish exposed to Profenofos free water for the same period that they were exposed to Profenofos. Our results revealed that with the progression of time and concentration, fish exposed to Profenofos showed significantly (p
