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Sandro Palmieri - One of the best experts on this subject based on the ideXlab platform.
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isolation and biochemical characterization of a basic myrosinase from ripe crambe abyssinica seeds highly specific for epi Progoitrin
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Roberta Bernardi, Michelina G Finiguerra, And Alessandro A Rossi, Sandro PalmieriAbstract:On the basis of previous studies on the mechanism-based inhibition, activation, and active site structure of myrosinase(s) isolated from Sinapis alba and other cruciferous seeds, crambe myrosinase shows uncommon properties and behavior. For this reason homogeneous crambe myrosinase was isolated and investigated to establish the most important physicochemical features, including kinetic properties determined with the epimers Progoitrin (R) and epi-Progoitrin (S) as substrates, with and without ascorbate as an activator. The results of this study demonstrate that crambe myrosinase is highly specific for epi-Progoitrin due to a better stabilization of the enzyme-substrate complex. This stabilization is caused by additional hydrogen bonding that only epi-Progoitrin can set up between its hydroxyl group and a suitable residue in the hydrophobic pocket where the "docking" of the glucosinolates side chain takes place.
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Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil
Pest Management Science, 2000Co-Authors: Luisa M. Manici, Onofrio Leoni, Luca Lazzeri, G. Baruzzi, Stefania Galletti, Sandro PalmieriAbstract:Glucosinolate degradation products (GLDPs), mainly isothiocyanates, obtained by myrosinase-catalysed hydrolysis of glucosinolates (GLs), are an important group of natural bioactive substances. The fungitoxic activity of four GLDPs, chosen on the basis of their side-chain structures, was assayed against Pythium irregulare and Rhizoctonia solani. The effectiveness of the degradation products (DPs) of sinigrin (alkenyl GL), epi-Progoitrin (hydroxy-alkenyl GL), glucoiberin and glucoerucin (thiofunctionalised GLs) in inhibiting P irregulare oospore germination and R solani soil colonisation were tested in a closed system, using an artificially infected soil. The fungitoxic activity of these GLDPs varied according to their side-chain structure. As in previous in vitro studies, the thiofunctionalised GLDPs were found the most effective, producing complete inhibition of P irregulare oospore germination (0.01 µmole g−1 soil) and R solani soil colonisation (0.5 µmole g−1 soil), but even sinigrin DP showed a fungitoxic activity higher than that of epi-Progoitrin DP. © 2000 Society of Chemical Industry
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In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables.
Journal of agricultural and food chemistry, 2000Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:A comparison of the effect of isothiocyanates and nitriles derived from some glucosinolates, namely, epi-Progoitrin, sinalbin, glucotropaeolin, glucocheirolin, and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocyanates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocyanates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-Progoitrin, glucotropaeolin, glucoraphenin, and glucocheirolin; while for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin-derived product. Considering the antiproliferative activity found for isothiocyanates and nitriles, further studies will be aimed to the possible application of glucosinolate-derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
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Crambe abyssinica meal as starting material for the production of enantiomerically pure fine chemicals
Industrial Crops and Products, 1998Co-Authors: P Daubos, Valérie Grumel, R Iori, Onofrio Leoni, Sandro Palmieri, Patrick RollinAbstract:Epi-Progoitrin, the major glucosinolate of Crambe abyssinica, was readily extracted from defatted crambe meal. A simple biotechnological process using immobilized myrosinase allowed a high-yielding chemo- and stereocontrolled transformation of epi-Progoitrin into epi-goitrin, which was obtained in pure stereochemical form. Many diverse chemical modifications can be envisaged on the derived epi-goitrin which should be considered as a novel sophisticated starting material for the production of enantiomerically pure fine chemicals.
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Myrosinase-generated isothiocyanate from glucosinolates: isolation, characterization and in vitro antiproliferative studies.
Bioorganic & medicinal chemistry, 1997Co-Authors: Onofrio Leoni, Sandro Palmieri, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Claudio NastruzziAbstract:Epidemiological and pharmacological studies have shown that colorectal cancer development could be reduced by consuming vegetables that contain glucosinolates. In view of this the effect of some glucosinolates and their isothiocyanate (ITC)-derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS, and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells has been investigated by using two alternative approaches: the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either of these methods. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs' inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, Progoitrin, epi-Progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with a different origin and hystotype. Considering the antiproliferative activity found for ITCs these compounds could be considered potentially responsible for the reduction of colorectal cancer associated with diets rich in cruciferous vegetables. Further studies will be aimed at the possible application of glucosinolate-derived products as chemopreventive cancer agents.
Onofrio Leoni - One of the best experts on this subject based on the ideXlab platform.
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Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil
Pest Management Science, 2000Co-Authors: Luisa M. Manici, Onofrio Leoni, Luca Lazzeri, G. Baruzzi, Stefania Galletti, Sandro PalmieriAbstract:Glucosinolate degradation products (GLDPs), mainly isothiocyanates, obtained by myrosinase-catalysed hydrolysis of glucosinolates (GLs), are an important group of natural bioactive substances. The fungitoxic activity of four GLDPs, chosen on the basis of their side-chain structures, was assayed against Pythium irregulare and Rhizoctonia solani. The effectiveness of the degradation products (DPs) of sinigrin (alkenyl GL), epi-Progoitrin (hydroxy-alkenyl GL), glucoiberin and glucoerucin (thiofunctionalised GLs) in inhibiting P irregulare oospore germination and R solani soil colonisation were tested in a closed system, using an artificially infected soil. The fungitoxic activity of these GLDPs varied according to their side-chain structure. As in previous in vitro studies, the thiofunctionalised GLDPs were found the most effective, producing complete inhibition of P irregulare oospore germination (0.01 µmole g−1 soil) and R solani soil colonisation (0.5 µmole g−1 soil), but even sinigrin DP showed a fungitoxic activity higher than that of epi-Progoitrin DP. © 2000 Society of Chemical Industry
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In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables.
Journal of agricultural and food chemistry, 2000Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:A comparison of the effect of isothiocyanates and nitriles derived from some glucosinolates, namely, epi-Progoitrin, sinalbin, glucotropaeolin, glucocheirolin, and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocyanates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocyanates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-Progoitrin, glucotropaeolin, glucoraphenin, and glucocheirolin; while for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin-derived product. Considering the antiproliferative activity found for isothiocyanates and nitriles, further studies will be aimed to the possible application of glucosinolate-derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
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Crambe abyssinica meal as starting material for the production of enantiomerically pure fine chemicals
Industrial Crops and Products, 1998Co-Authors: P Daubos, Valérie Grumel, R Iori, Onofrio Leoni, Sandro Palmieri, Patrick RollinAbstract:Epi-Progoitrin, the major glucosinolate of Crambe abyssinica, was readily extracted from defatted crambe meal. A simple biotechnological process using immobilized myrosinase allowed a high-yielding chemo- and stereocontrolled transformation of epi-Progoitrin into epi-goitrin, which was obtained in pure stereochemical form. Many diverse chemical modifications can be envisaged on the derived epi-goitrin which should be considered as a novel sophisticated starting material for the production of enantiomerically pure fine chemicals.
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Myrosinase-generated isothiocyanate from glucosinolates: isolation, characterization and in vitro antiproliferative studies.
Bioorganic & medicinal chemistry, 1997Co-Authors: Onofrio Leoni, Sandro Palmieri, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Claudio NastruzziAbstract:Epidemiological and pharmacological studies have shown that colorectal cancer development could be reduced by consuming vegetables that contain glucosinolates. In view of this the effect of some glucosinolates and their isothiocyanate (ITC)-derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS, and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells has been investigated by using two alternative approaches: the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either of these methods. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs' inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, Progoitrin, epi-Progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with a different origin and hystotype. Considering the antiproliferative activity found for ITCs these compounds could be considered potentially responsible for the reduction of colorectal cancer associated with diets rich in cruciferous vegetables. Further studies will be aimed at the possible application of glucosinolate-derived products as chemopreventive cancer agents.
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In Vitro Cytotoxic Activity of Some Glucosinolate-Derived Products Generated by Myrosinase Hydrolysis
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:The effects of glucosinolates (GLs) (sinigrin, gluconapin, Progoitrin, epi-Progoitrin, sinalbin, glucotropaeolin, glucoerucin, glucocheirolin, and glucoraphenin) and their enzymatic hydrolysis-derived products (EHDPs) have been studied in controlling the proliferation of cancer cell lines. The results of this study indicate the following: (i) neither myrosinase nor intact GLs have any effect on tumor cell growth when used up to 36 U/mL and 500 μM, respectively; (ii) all EHDPs show a clear inhibition of human erythroleukemic K562 cell proliferative growth, which is particularly evident for EHDPs from sinigrin, glucotropaeolin, glucoerucin, and glucocheirolin (IC50 < 20 μM); (iii) the EHDP production by in situ or pre-mix procedures gives rather similar antiproliferative effects; and finally, (iv) the EHDPs from glucoraphenin are active toward several other tumor cells, viz. FL (murine erythroleukemic cells), Jurkat (human T-lymphoid cells), HeLa (human cervix carcinoma cells), H9 (human T-lymphoid cells),...
Claudio Nastruzzi - One of the best experts on this subject based on the ideXlab platform.
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In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables.
Journal of agricultural and food chemistry, 2000Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:A comparison of the effect of isothiocyanates and nitriles derived from some glucosinolates, namely, epi-Progoitrin, sinalbin, glucotropaeolin, glucocheirolin, and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocyanates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocyanates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-Progoitrin, glucotropaeolin, glucoraphenin, and glucocheirolin; while for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin-derived product. Considering the antiproliferative activity found for isothiocyanates and nitriles, further studies will be aimed to the possible application of glucosinolate-derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
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Myrosinase-generated isothiocyanate from glucosinolates: isolation, characterization and in vitro antiproliferative studies.
Bioorganic & medicinal chemistry, 1997Co-Authors: Onofrio Leoni, Sandro Palmieri, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Claudio NastruzziAbstract:Epidemiological and pharmacological studies have shown that colorectal cancer development could be reduced by consuming vegetables that contain glucosinolates. In view of this the effect of some glucosinolates and their isothiocyanate (ITC)-derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS, and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells has been investigated by using two alternative approaches: the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either of these methods. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs' inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, Progoitrin, epi-Progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with a different origin and hystotype. Considering the antiproliferative activity found for ITCs these compounds could be considered potentially responsible for the reduction of colorectal cancer associated with diets rich in cruciferous vegetables. Further studies will be aimed at the possible application of glucosinolate-derived products as chemopreventive cancer agents.
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In Vitro Cytotoxic Activity of Some Glucosinolate-Derived Products Generated by Myrosinase Hydrolysis
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:The effects of glucosinolates (GLs) (sinigrin, gluconapin, Progoitrin, epi-Progoitrin, sinalbin, glucotropaeolin, glucoerucin, glucocheirolin, and glucoraphenin) and their enzymatic hydrolysis-derived products (EHDPs) have been studied in controlling the proliferation of cancer cell lines. The results of this study indicate the following: (i) neither myrosinase nor intact GLs have any effect on tumor cell growth when used up to 36 U/mL and 500 μM, respectively; (ii) all EHDPs show a clear inhibition of human erythroleukemic K562 cell proliferative growth, which is particularly evident for EHDPs from sinigrin, glucotropaeolin, glucoerucin, and glucocheirolin (IC50 < 20 μM); (iii) the EHDP production by in situ or pre-mix procedures gives rather similar antiproliferative effects; and finally, (iv) the EHDPs from glucoraphenin are active toward several other tumor cells, viz. FL (murine erythroleukemic cells), Jurkat (human T-lymphoid cells), HeLa (human cervix carcinoma cells), H9 (human T-lymphoid cells),...
Renato Iori - One of the best experts on this subject based on the ideXlab platform.
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In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables.
Journal of agricultural and food chemistry, 2000Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:A comparison of the effect of isothiocyanates and nitriles derived from some glucosinolates, namely, epi-Progoitrin, sinalbin, glucotropaeolin, glucocheirolin, and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocyanates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocyanates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-Progoitrin, glucotropaeolin, glucoraphenin, and glucocheirolin; while for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin-derived product. Considering the antiproliferative activity found for isothiocyanates and nitriles, further studies will be aimed to the possible application of glucosinolate-derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
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Myrosinase-generated isothiocyanate from glucosinolates: isolation, characterization and in vitro antiproliferative studies.
Bioorganic & medicinal chemistry, 1997Co-Authors: Onofrio Leoni, Sandro Palmieri, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Claudio NastruzziAbstract:Epidemiological and pharmacological studies have shown that colorectal cancer development could be reduced by consuming vegetables that contain glucosinolates. In view of this the effect of some glucosinolates and their isothiocyanate (ITC)-derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS, and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells has been investigated by using two alternative approaches: the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either of these methods. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs' inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, Progoitrin, epi-Progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with a different origin and hystotype. Considering the antiproliferative activity found for ITCs these compounds could be considered potentially responsible for the reduction of colorectal cancer associated with diets rich in cruciferous vegetables. Further studies will be aimed at the possible application of glucosinolate-derived products as chemopreventive cancer agents.
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In Vitro Cytotoxic Activity of Some Glucosinolate-Derived Products Generated by Myrosinase Hydrolysis
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:The effects of glucosinolates (GLs) (sinigrin, gluconapin, Progoitrin, epi-Progoitrin, sinalbin, glucotropaeolin, glucoerucin, glucocheirolin, and glucoraphenin) and their enzymatic hydrolysis-derived products (EHDPs) have been studied in controlling the proliferation of cancer cell lines. The results of this study indicate the following: (i) neither myrosinase nor intact GLs have any effect on tumor cell growth when used up to 36 U/mL and 500 μM, respectively; (ii) all EHDPs show a clear inhibition of human erythroleukemic K562 cell proliferative growth, which is particularly evident for EHDPs from sinigrin, glucotropaeolin, glucoerucin, and glucocheirolin (IC50 < 20 μM); (iii) the EHDP production by in situ or pre-mix procedures gives rather similar antiproliferative effects; and finally, (iv) the EHDPs from glucoraphenin are active toward several other tumor cells, viz. FL (murine erythroleukemic cells), Jurkat (human T-lymphoid cells), HeLa (human cervix carcinoma cells), H9 (human T-lymphoid cells),...
Enea Menegatti - One of the best experts on this subject based on the ideXlab platform.
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In vitro antiproliferative activity of isothiocyanates and nitriles generated by myrosinase-mediated hydrolysis of glucosinolates from seeds of cruciferous vegetables.
Journal of agricultural and food chemistry, 2000Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:A comparison of the effect of isothiocyanates and nitriles derived from some glucosinolates, namely, epi-Progoitrin, sinalbin, glucotropaeolin, glucocheirolin, and glucoraphenin, on human erythroleukemic in vitro cultured cells was studied. Many studies have in fact evidenced that a consumption of vegetable containing glucosinolates could reduce the development of colorectal cancer. In the experimental conditions used, the production of isothiocyanates and nitriles from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The obtained results demonstrated that in general nitriles are considerably less potent than the corresponding isothiocyanates in inhibiting cancer cell growth. Particularly, the isothiocyanates inhibitory activity on K562 cells growth is higher in the case of products derived from epi-Progoitrin, glucotropaeolin, glucoraphenin, and glucocheirolin; while for nitriles the higher activity in inhibiting K562 cells growth is showed by sinalbin-derived product. Considering the antiproliferative activity found for isothiocyanates and nitriles, further studies will be aimed to the possible application of glucosinolate-derived products as chemopreventive cancer agents for the reduction of colorectal cancer.
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Myrosinase-generated isothiocyanate from glucosinolates: isolation, characterization and in vitro antiproliferative studies.
Bioorganic & medicinal chemistry, 1997Co-Authors: Onofrio Leoni, Sandro Palmieri, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Claudio NastruzziAbstract:Epidemiological and pharmacological studies have shown that colorectal cancer development could be reduced by consuming vegetables that contain glucosinolates. In view of this the effect of some glucosinolates and their isothiocyanate (ITC)-derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS, and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells has been investigated by using two alternative approaches: the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either of these methods. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs' inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, Progoitrin, epi-Progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with a different origin and hystotype. Considering the antiproliferative activity found for ITCs these compounds could be considered potentially responsible for the reduction of colorectal cancer associated with diets rich in cruciferous vegetables. Further studies will be aimed at the possible application of glucosinolate-derived products as chemopreventive cancer agents.
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In Vitro Cytotoxic Activity of Some Glucosinolate-Derived Products Generated by Myrosinase Hydrolysis
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Claudio Nastruzzi, Onofrio Leoni, Rita Cortesi, Elisabetta Esposito, Enea Menegatti, Renato Iori, Sandro PalmieriAbstract:The effects of glucosinolates (GLs) (sinigrin, gluconapin, Progoitrin, epi-Progoitrin, sinalbin, glucotropaeolin, glucoerucin, glucocheirolin, and glucoraphenin) and their enzymatic hydrolysis-derived products (EHDPs) have been studied in controlling the proliferation of cancer cell lines. The results of this study indicate the following: (i) neither myrosinase nor intact GLs have any effect on tumor cell growth when used up to 36 U/mL and 500 μM, respectively; (ii) all EHDPs show a clear inhibition of human erythroleukemic K562 cell proliferative growth, which is particularly evident for EHDPs from sinigrin, glucotropaeolin, glucoerucin, and glucocheirolin (IC50 < 20 μM); (iii) the EHDP production by in situ or pre-mix procedures gives rather similar antiproliferative effects; and finally, (iv) the EHDPs from glucoraphenin are active toward several other tumor cells, viz. FL (murine erythroleukemic cells), Jurkat (human T-lymphoid cells), HeLa (human cervix carcinoma cells), H9 (human T-lymphoid cells),...
