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Jennwen Huang - One of the best experts on this subject based on the ideXlab platform.
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determination of Sinigrin in vegetable seeds by online microdialysis sampling coupled to reverse phase ion pair liquid chromatography
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Tsaihung Lin, Jennwen Huang, Ponnusamy Vinoth Kumar, Jenfon JenAbstract:A hollow fiber microdialysis sampling coupled online to ion-pair liquid chromatography was investigated as an alternative to sample pretreatment for the direct determination of Sinigrin in cruciferous vegetables without desulfation. After microdialysis, the dialysate was online injected into the chromatographic system to analyze the Sinigrin with UV detection at 227 nm. Parameters affecting the microdialysis efficiency, such as flow rate, polarity modifier, pH in perfusion stream, pH, and salt added in sample solution, were studied. Through ion suppression in the donor phase and ion-pair formation in the acceptor phase, the microdialysis efficiency of Sinigrin was enhanced. Experimental results revealed that the microdialysis of a sample solution (pH 2.0) using 0.1 M tetrabutylammonium (pH 12) as a perfusate at 10 μL/min flow rate maximized the extraction efficiency. Detection was linear in the concentration of 1.0−100 mg/L with a detection limit of 0.3 mg/L. Three seed samples were analyzed, with sinigri...
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direct determination of Sinigrin in mustard seed without desulfatation by reversed phase ion pair liquid chromatography
Journal of Chromatography A, 2001Co-Authors: Jennwen Huang, Wenchuan ChungAbstract:Reversed-phase ion-pair liquid chromatography has been investigated for directly analyzing Sinigrin in mustard seed without desulfatation. After extraction by phosphate buffer (pH 7.0) from the grind-pastes of inactivated-myrosinase mustard seeds, Sinigrin was first isolated through deproteinization and centrifugation, followed by filtration and injection into the chromatographic system. A reversed-phase C18 column was used to separate the Sinigrin with an eluent of acetonitrile (ACN)–water (20:80) containing 0.02 M tetrabutylammonium (TBA) as the counter ion at pH 7.0. Detection was carried out with an UV detector operated at 227 nm. Factors affecting the chromatographic separation and quantitative determination, such as concentrations of TBA and ACN, and pH, were studied. The linear dynamic range is larger than three orders of magnitude and the detection limit is 0.045 mg/L. The RSD is around 3% and the recovery is 85% (3% RSD, n=3).
Qipeng Yuan - One of the best experts on this subject based on the ideXlab platform.
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concentration of Sinigrin from indian mustard brassica juncea l seeds using nanofiltration membrane
2015Co-Authors: Tianxin Wang, Hao Liang, Qipeng YuanAbstract:Sinigrin has drawn more attention of pathologists and immunologists, which exhibits a wide range of biological activities. In the present study, the feasibility of concentration of Sinigrin using nanofiltration membranes was studied using one-factor-at-a-time method with the permeate flux and Sinigrin retention as responses. After seven commercial membranes were compared, including NF, NF-270, SPESI, STARMEN 120, STARMEN 128, STARMEN 220, STARMEN 240, we investigated the effects of temperature, pressure, and feed concentration on the concentration process of Sinigrin from crude aqueous extracts of Indian mustard (Brassica juncea L.) seeds followed by primary ultrafiltration. The results indicated that NF-270 membrane showed the best concentration capability to Sinigrin. Their ranges of permeate flux and Sinigrin retention were 7.44–23.47 L/(m2 h) and 90.77–97.13 %, respectively. Furthermore, the high flux and high retention were explained by swelling ratio, SEM, and ATR-FTIR, which were applied to determine the differences of nanofiltration membrane before and after soaking in aqueous solution. Finally, it could be concluded that the concentration process using nanofiltration provided a novel, rapid, and economical method for concentration of Sinigrin.
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Separation and Purification of Sinigrin and Gluconapin from Defatted Indian Mustard Seed Meals by Macroporous Anion Exchange Resin and Medium Pressure Liquid Chromatography
Separation Science and Technology, 2014Co-Authors: Tianxin Wang, Hao Liang, Qipeng YuanAbstract:Defatted seed meals, the byproducts of Indian mustard seed oil industry, which are just used as animal feedstock and nitrogen fertilizers, possess various nutrients and phytochemical compounds, such as Sinigrin and gluconapin. The present study demonstrated a novel, low-cost, and efficient method for the co-production of Sinigrin and gluconapin from defatted Indian mustard seed meals by D261 strong basic anion-exchange macroporous resin and medium pressure liquid chromatography (MPLC). The adsorption/desorption capacities of eight adsorbents characterized by BET, IR, and EDS were screened. 26.4 g defatted Indian mustard seed meals, which contained about 1210.3 mg Sinigrin and 696.6 mg gluconapin, could produce 2059.6 mg of glucosinolate-rich extract of 53.67% Sinigrin and 31.30% gluconapin after separation by D261 resin. Then, 949.40 mg of 97% Sinigrin and 568.40 mg of 95% gluconapin could be obtained after the extracts were purified by MPLC, with the recovery of 76.03% of Sinigrin and 77.38% of gluconapi...
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separation of Sinigrin from indian mustard brassica juncea l seed using macroporous ion exchange resin
Korean Journal of Chemical Engineering, 2012Co-Authors: Tianxin Wang, Hao Liang, Qipeng YuanAbstract:Sinigrin is a major glucosinolate present in Indian mustard (Brassica juncea L.) seeds as the precursor of the anticancer compound allyl isothiocyanate. In the present study, the adsorption and desorption characteristics of six macroporous ion-exchange resins for the separation of Sinigrin from crude aqueous extracts have been compared. The results indicated that D261 resin showed the best adsorption and desorption capacity to Sinigrin, and its adsorption data fit best to the Freundlich isotherm. The dynamic adsorption/desorption experiments were carried out to optimize the separation process. After treatment with D261 resin in one run, the purity of Sinigrin in the product was increased 15.57-fold from 3.75% to 58.37% with the recovery of 79.82%. Meanwhile, the separation effect of D261 resin was also supported by UV and IR. The separation process using macroporous ion-exchange resin in our paper provides a novel, rapid and economical method for separation of Sinigrin.
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optimization of ultrasonic stimulated solvent extraction of Sinigrin from indian mustard seed brassica juncea l using response surface methodology
Phytochemical Analysis, 2011Co-Authors: Tianxin Wang, Hao Liang, Qipeng YuanAbstract:Introduction – Sinigrin, a major glucosinolate present in Indian mustard (Brassica juncea L.) seeds as the precursor of the anticancer compound allyl isothiocyanate, shows a wide range of biological activities. It's necessary to optimize the extraction methods and conditions, in order to improve the extraction productivity and save raw material. Objective – To systemically investigate and optimize the most important factors affected the productivity of Sinigrin in the process of extraction using response surface methodology. Methodology – The ranges of three main factors including the ethanol concentration, extraction time and extraction temperature were selected by the one-factor-at-a-time method. The conditions of ultrasonic-stimulated extraction of Sinigrin from defatted Indian mustard seed powder were optimized by Box-Behnken design to obtain the maximum productivity. Result – The predicted productivity (3.81%) was obtained using 57% ethanol concentration at 81°C for 60 min, with the coefficient of the model R2 > 0.96 (n = 17). The actual productivity (3.84 ± 0.02%) of Sinigrin under the optimized condition was increased by 70.67% compared with the result of conventional extraction. Meanwhile, HPLC, UV and IR were applied to examine if there is a difference between the ultrasonic-stimulated solvent extraction and conventional extraction, and the improvement of productivity of Sinigrin depended on the destruction of cell wall caused by the elimination of outer pectinous material was explained by SEM and composition content analysis. Conclusion – The ultrasonic-stimulated solvent extraction was suggested to be a promising method to improve the productivity of Sinigrin. And the results demonstrated that Sinigrin productivity may be related to pectinous materials existed in the seeds. Copyright © 2010 John Wiley & Sons, Ltd.
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optimization of ultrasonic stimulated solvent extraction of Sinigrin from indian mustard seed brassica juncea l using response surface methodology
Phytochemical Analysis, 2011Co-Authors: Tianxin Wang, Hao Liang, Qipeng YuanAbstract:Introduction – Sinigrin, a major glucosinolate present in Indian mustard (Brassica juncea L.) seeds as the precursor of the anticancer compound allyl isothiocyanate, shows a wide range of biological activities. It's necessary to optimize the extraction methods and conditions, in order to improve the extraction productivity and save raw material. Objective – To systemically investigate and optimize the most important factors affected the productivity of Sinigrin in the process of extraction using response surface methodology. Methodology – The ranges of three main factors including the ethanol concentration, extraction time and extraction temperature were selected by the one-factor-at-a-time method. The conditions of ultrasonic-stimulated extraction of Sinigrin from defatted Indian mustard seed powder were optimized by Box-Behnken design to obtain the maximum productivity. Result – The predicted productivity (3.81%) was obtained using 57% ethanol concentration at 81°C for 60 min, with the coefficient of the model R2 > 0.96 (n = 17). The actual productivity (3.84 ± 0.02%) of Sinigrin under the optimized condition was increased by 70.67% compared with the result of conventional extraction. Meanwhile, HPLC, UV and IR were applied to examine if there is a difference between the ultrasonic-stimulated solvent extraction and conventional extraction, and the improvement of productivity of Sinigrin depended on the destruction of cell wall caused by the elimination of outer pectinous material was explained by SEM and composition content analysis. Conclusion – The ultrasonic-stimulated solvent extraction was suggested to be a promising method to improve the productivity of Sinigrin. And the results demonstrated that Sinigrin productivity may be related to pectinous materials existed in the seeds. Copyright © 2010 John Wiley & Sons, Ltd.
Wenchuan Chung - One of the best experts on this subject based on the ideXlab platform.
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direct determination of Sinigrin in mustard seed without desulfatation by reversed phase ion pair liquid chromatography
Journal of Chromatography A, 2001Co-Authors: Jennwen Huang, Wenchuan ChungAbstract:Reversed-phase ion-pair liquid chromatography has been investigated for directly analyzing Sinigrin in mustard seed without desulfatation. After extraction by phosphate buffer (pH 7.0) from the grind-pastes of inactivated-myrosinase mustard seeds, Sinigrin was first isolated through deproteinization and centrifugation, followed by filtration and injection into the chromatographic system. A reversed-phase C18 column was used to separate the Sinigrin with an eluent of acetonitrile (ACN)–water (20:80) containing 0.02 M tetrabutylammonium (TBA) as the counter ion at pH 7.0. Detection was carried out with an UV detector operated at 227 nm. Factors affecting the chromatographic separation and quantitative determination, such as concentrations of TBA and ACN, and pH, were studied. The linear dynamic range is larger than three orders of magnitude and the detection limit is 0.045 mg/L. The RSD is around 3% and the recovery is 85% (3% RSD, n=3).
Jenfon Jen - One of the best experts on this subject based on the ideXlab platform.
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determination of Sinigrin in vegetable seeds by online microdialysis sampling coupled to reverse phase ion pair liquid chromatography
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Tsaihung Lin, Jennwen Huang, Ponnusamy Vinoth Kumar, Jenfon JenAbstract:A hollow fiber microdialysis sampling coupled online to ion-pair liquid chromatography was investigated as an alternative to sample pretreatment for the direct determination of Sinigrin in cruciferous vegetables without desulfation. After microdialysis, the dialysate was online injected into the chromatographic system to analyze the Sinigrin with UV detection at 227 nm. Parameters affecting the microdialysis efficiency, such as flow rate, polarity modifier, pH in perfusion stream, pH, and salt added in sample solution, were studied. Through ion suppression in the donor phase and ion-pair formation in the acceptor phase, the microdialysis efficiency of Sinigrin was enhanced. Experimental results revealed that the microdialysis of a sample solution (pH 2.0) using 0.1 M tetrabutylammonium (pH 12) as a perfusate at 10 μL/min flow rate maximized the extraction efficiency. Detection was linear in the concentration of 1.0−100 mg/L with a detection limit of 0.3 mg/L. Three seed samples were analyzed, with sinigri...
B K Mitchell - One of the best experts on this subject based on the ideXlab platform.
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deterrency and the variable caterpillar trichoplusia ni and Sinigrin
Entomologia Experimentalis Et Applicata, 1996Co-Authors: B K Mitchell, K A Justus, Kiyoshi AsaokaAbstract:Shields & Mitchell (1995a) demonstrated that Sinigrin, a simple glucosinolate found in some host plants of Trichoplusia ni (Hubner), acts as a feeding deterrent for this insect at ecologically meaningful concentrations, and that feeding stimulants can modulate the effect of such deterrents. They also showed that cells sensitive to Sinigrin are present in T. ni and that the activity of these cells is correlated with feeding behaviour. They concluded that Sinigrin is not a feeding stimulant for the cabbage looper but that it does act as a deterrent under certain conditions (Shields & Mitchell, 1995b, c). Despite the fact that correlations could be made between sensory input and behavioural output, individual insects were observed that were significant outliers. The most obvious of these were larvae that fed normally on agar disks containing very high (up to 20 mM) Sinigrin.
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Sinigrin as a feeding deterrent in two crucifer feeding polyphagous lepidopterous species and the effects of feeding stimulant mixtures on deterrency
Philosophical Transactions of the Royal Society B, 1995Co-Authors: V D C Shields, B K MitchellAbstract:The glucosinolate, Sinigrin (allyl- or 2-propenyl glucosinolate), present in several Gruciferae, was incorporated in varying concentrations into four different diet background mixtures to study the behavioural reactions of Mamestra configurata and Trichoplusia ni. Sinigrin concentrations were chosen to mimic normal levels in naturally occurring cruciferous plants, or to represent a plant during a particular stage in its growth cycle. One diet background mixture contained potassium chloride (KC1) and no stimulatory sugar or sugar alcohol, two backgrounds contained KC1 and a single sugar or sugar alcohol (sucrose or inositol, respectively), and the fourth background contained KC1 and both sugar and sugar alcohol (sucrose and inositol, respectively). Sinigrin acted primarily to reduce (deter) feeding in all backgrounds, although the effect varied with Sinigrin concentration, background and species. When inositol or sucrose was included in the mixture, the deterrent effect of Sinigrin was decreased in both species. When inositol and sucrose were present, suppression of the deterrent effect of Sinigrin was greatest for M. configurata . The effects of mixtures were not predictable from a knowledge of the action of individual components. Differences observed between species may reflect different sensory capabilities.
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responses of maxillary styloconic receptors to stimulation by Sinigrin sucrose and inositol in two crucifer feeding polyphagous lepidopterous species
Philosophical Transactions of the Royal Society B, 1995Co-Authors: V D C Shields, B K MitchellAbstract:Chemosensory cells in the lateral and the medial styloconic sensillia on the galea of larval Mamestra configurata and Trichoplusia ni were investigated electrophysiologically. Sinigrin, sucrose, inositol and potassium chloride (KCl) were tested on both sensilla of each species. One of the four cells in both the lateral and medial sensilla of T. ni was Sinigrin-sensitive, whereas in M. configurata, one cell in only the lateral sensillum was Sinigrin-sensitive. The lateral Sinigrin-sensitive cell of T. ni was eightfold more sensitive than the corresponding cell of M. configurata. One cell in the lateral sensillum of both species was sucrose-sensitive. The medial sensillum of M. configurata housed an inositol-sensitive cell but no cell sensitive to inositol was present in T. ni. One cell in the lateral and medial sensilla of both species was KCl-sensitive. Adaptation and dose-response data are given for the Sinigrin-sensitive cells in both species. These are discussed in the context of deterrent-sensitive cells reported from other Lepidoptera. Comparative physiological and evolutionary aspects are considered and the general concepts of the lepidopteran deterrent cell is discussed.