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Hans-peter Klenk - One of the best experts on this subject based on the ideXlab platform.
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Dissecting the taxonomic heterogeneity within Propionibacterium acnes : proposal for Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Itaru Dekio, Renata Culak, Raju Misra, Tom Gaulton, Min Fang, Mitsuo Sakamoto, Moriya Ohkuma, Kenshiro Oshima, Masahira Hattori, Hans-peter KlenkAbstract:Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, l-pyrrolidonyl arylamidase or d-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T ( = ATCC 6919T = JCM 6425T = DSM 1897T = CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T ( = NCTC 13655T = JCM 18919T).
Raniarifiyanty Nabella - One of the best experts on this subject based on the ideXlab platform.
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Uji Aktivitas Antibakteri Fraksi Etil Asetat Kulit Buah Citrus reticulata terhadap Propionibacterium acnes dengan menggunakan Metode Difusi Cakram
2020Co-Authors: Raniarifiyanty NabellaAbstract:Background: Acne vulgaris (AV) is a chronic inflammation caused by the abnormalities of polisebaceous duct. One of pathogenesis of acne is the colonization of Propionibacterium acnes that caused inflammation. The first line therapy used for acne vulgaris treatment is antibiotics that might have many unwanted side effects including antibiotic resistance. Citrus reticulata proven to have many pharmacologic benefits including antibacterial therapy. Objective: The aim of this study is to determine the antibacterial activity of ethyl acetate fraction of Citrus reticulata peel against Propionibacterium acnes. Method: Antibacterial activity test that is performed using disc diffusion method. The first step is fractionation. And then phytochemical screening was performed to identify the chemical compounds. Antibacterial activity test is performed using 2,5%, 6,5%, and 12,5% concentration. The disc containing test solution, positive control and negative control were placed at plate that already containing P. acnes and MHA. Then it is incubated at 37°C for 24 hours and then inhibition zone formed is measured. Results and conclusion: The average diameter measured from inhibition zone of antibacterial activity of ethyl acetate fraction Citrus reticulata peel concentration 2.5% is 6.90mm, contentration 6.5% is 7.95mm, and contentration 12.5% is 9.36mm. The result shows that ethyl acetate fraction of Citrus reticulata has potential as antibacterial therapy starting from the lowest concentration
Andreas F Widmer - One of the best experts on this subject based on the ideXlab platform.
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Propionibacterium acnes prosthetic valve endocarditis with abscess formation a case report
BMC Infectious Diseases, 2014Co-Authors: Mario Kurz, Beat A Kaufmann, Larry M Baddour, Andreas F WidmerAbstract:Background Endocarditis due to Propionibacterium acnes is a rare disease. Scant data on treatment of these infections is available and is based on case reports only. If the disease is complicated by abscess formation, surgical intervention combined with an antibiotic therapy might improve clinical outcome. In some cases, cardiac surgeons are reluctant to perform surgery, since they consider the intervention as high risk. Therefore, a conservative therapy is required, with little, if any evidence to choose the optimal antibiotic. We report the first case of a successfully treated patient with P. acnes prosthetic valve endocarditis without surgery.
Askarani S Nurul - One of the best experts on this subject based on the ideXlab platform.
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UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA KULIT BUAH Citrus reticulata TERHADAP Propionibacterium acnes DENGAN MENGGUNAKAN METODE DIFUSI CAKRAM
2019Co-Authors: Askarani S NurulAbstract:Background: Citrus reticulata peel has proven to inhibit the growth of Propionibacterium acnes with secondary metabolites which include alkaloids, flavonoids, anthraquinones, and polyphenols. Objective: The aim of this research was observing at the inhibitory zone of Citrus reticulata fruit peel fraction against the growth of Propionibacterium acnes with different concentrations. Method: This research applied antibacterial activity test using the disc diffusion method. The n-hexane fraction used in this study was obtained from previous studies. The antibacterial activity test was conducted with the concentration of n-hexane fraction used 25 mg/ml; 65 mg/ml; 125 mg/ml; 250 mg/ml; and 500 mg/ml. The disc containing the test solution, negative control, and positive control was affixed to the plate which contained Propionibacterium acnes and MHA media, then incubated at 37° C for ± 24 hours and the clear zone formed was measured in diameter. Results : The results of the secondary metabolite identification in the previous study showed that n-hexane fascination of Citrus reticulata fruit peel contained alkaloids, flavonoids, anthraquinones, and polyphenols. The results of testing the antibacterial activity at a concentration of 25 mg/ml, 65 mg/ml, 125 mg/ml and 250 mg did not provide a zone of inhibition while at a concentration of 500 mg/ml gave an inhibition zone of 13.5 mm. Conclutions : From the result of observation in this research it can be concluded that relatively the inhibition obtained fall into the category of resistant, so it’s not effective to work as an antibacterial
Itaru Dekio - One of the best experts on this subject based on the ideXlab platform.
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Dissecting the taxonomic heterogeneity within Propionibacterium acnes : proposal for Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Itaru Dekio, Renata Culak, Raju Misra, Tom Gaulton, Min Fang, Mitsuo Sakamoto, Moriya Ohkuma, Kenshiro Oshima, Masahira Hattori, Hans-peter KlenkAbstract:Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, l-pyrrolidonyl arylamidase or d-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T ( = ATCC 6919T = JCM 6425T = DSM 1897T = CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T ( = NCTC 13655T = JCM 18919T).
