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Vassya Bankova - One of the best experts on this subject based on the ideXlab platform.
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standard methods for apis mellifera Propolis research
Journal of Apicultural Research, 2019Co-Authors: Vassya Bankova, Soraia Falcão, Davide Bertelli, Renata S Borba, Bruno Jose Conti, Ildenize B S Cunha, Carolina Danert, Marcos N Eberlin, Maria Ines Isla, Mar Ia Ines Nieva MorenoAbstract:Propolis is one of the most fascinating honey bee (Apis mellifera L.) products. It is a plant derived product that bees produce from resins that they collect from different plant organs and with which they mix beeswax. Propolis is a building material and a protective agent in the bee hive. It also plays an important role in honey bee social immunity, and is widely used by humans as an ingredient of nutraceuticals, over-the-counter preparations and cosmetics. Its chemical composition varies by geographic location, climatic zone and local flora. The understanding of the chemical diversity of Propolis is very important in Propolis research. In this manuscript, we give an overview of the available methods for studying Propolis in different aspects: Propolis in the bee colony; chemical composition and plant sources of Propolis; biological activity of Propolis with respect to bees and humans; and approaches for standardization and quality control for the purposes of industrial application.
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Propolis volatile compounds: chemical diversity and biological activity: a review
Chemistry Central Journal, 2014Co-Authors: Vassya Bankova, Milena Popova, Boryana TrushevaAbstract:Propolis is a sticky material collected by bees from plants, and used in the hive as building material and defensive substance. It has been popular as a remedy in Europe since ancient times. Nowadays, Propolis use in over-the-counter preparations, “bio”-cosmetics and functional foods, etc., increases. Volatile compounds are found in low concentrations in Propolis, but their aroma and significant biological activity make them important for Propolis characterisation. Propolis is a plant-derived product: its chemical composition depends on the local flora at the site of collection, thus it offers a significant chemical diversity. The role of Propolis volatiles in identification of its plant origin is discussed. The available data about chemical composition of Propolis volatiles from different geographic regions are reviewed, demonstrating significant chemical variability. The contribution of volatiles and their constituents to the biological activities of Propolis is considered. Future perspectives in research on Propolis volatiles are outlined, especially in studying activities other than antimicrobial.
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Propolis: properties, application, and its potential.
Evidence-Based Complementary and Alternative Medicine, 2013Co-Authors: Wojciech Król, Vassya Bankova, José Maurício Sforcin, Ewelina Szliszka, Zenon P. Czuba, Andrzej K. KuropatnickiAbstract:Propolis is a honeybee product known for its biological and pharmacological properties for centuries. It has been extensively used in traditional medicine and also, because of its antibacterial, antiseptic, anti-inflammatory, and anesthetic activities, in complementary medicine. Propolis became subject of numerous studies developed and carried out all over the world in order to analyze its chemical composition as well as medicinal properties. The inspiration for this special issue was the tenth anniversary of death of Professor Stan Scheller, a precursor of Propolis research in Poland. For this special issue we invited investigators and scholars to submit original research reports and review articles as well as short communications on the topic of Propolis: its history, the history of the research on Propolis, chemical composition of Propolis, activity of Propolis, and application of Propolis in medicine, dentistry and veterinary medicine. More than 50 papers were submitted from which we have selected 41 which represent the rich and multifaceted knowledge. They cover a wide range of topics and are divided into review and research articles. The research articles provide background and are the starting point for discussion of research trends in general terms. They deal with historical aspects of Propolis research and focus on Professor Scheller's pioneer studies on Propolis that commenced in Poland in the early 1960s. It was Scheller and his team who developed a method of introducing ethanol extracts of Propolis into aqueous solutions. They showed that Propolis acts as antioxidant and radioprotector, stimulates regeneration of tissue, and has immunomodulatory properties. Another paper describes Scheller's achievements in applying Propolis in the treatment of burns, venous ulcerations, suppurative osteitis, and arthritis as well as postoperative wound complications. Some authors describe advances in the studies on chemical composition of Propolis as well as botanical sources resulting in its geographically conditioned diversity. In another paper the authors present developments in the analysis and pharmacological properties of Propolis which are the starting point for preparation standardization using as an example Romanian Propolis. Practical applications of Propolis in medicinal therapy and cosmetics are also reviewed. A separate group of papers deals with prophylactic and medicinal properties of Propolis preparations in the treatment of cardiovascular diseases, cancer, oral diseases, and wound healing. Most of the articles in this special issue are of research character. They present the results of a variety of studies comprising different Propolis extracts and their fractions as well as chemical compounds isolated from them. The articles describe experimental studies, both in vitro and in vivo, and clinical studies. One group of articles deals with anticancer properties of Propolis. The effect of caffeic acid phenethyl ester (CAPE) on epithelial-mesenchymal transition (EMT) of human pancreatic cancer cells was investigated. The authors conclude that CAPE could inhibit the orthotopic growth and EMT of pancreatic cancer cells. The assessment of cytotoxic action of geoPropolis produced by stingless bees on canine osteosarcoma cells showed that it was efficient against OSA cells in a dose- and time-dependent way. Bioactive fraction of geoPropolis was also shown to decrease neutrophils migration in inflammatory process. Other authors showed that nymphaeol-A, the major component of Okinawan Propolis, suppresses angiogenesis and that is why it may be a useful agent for preventing tumor-induced angiogenesis. In other studies, anticancer activity of the ethanol extract of Indian stingless bee Propolis was explored by testing the cytotoxic and apoptotic effects in four different cancer cell lines at different concentrations. It was demonstrated that antioxidant potential of Indian stingless bee Propolis substantiates its anticancer activity. Another group of articles deals with anti-inflammatory and immunomodulatory activity of Propolis. Some researchers showed that Brazilian green Propolis and Chinese (poplar type) Propolis demonstrate anti-inflammatory property. Brazilian green Propolis extract proved to be effective in regulating inflammasomes which are formed in the cell cystol in response to stress signals, toxins, and microbial infections. It also showed a direct action against parasite and displayed immunomodulatory effects on murine macrophages. A separate group of articles deals with wound healing. Propolis proved effective in decreasing the amount of free radicals in burn wounds. Propolis burn treatment led to enhanced collagens and its components expression. Application of Propolis ointment for topical treatment of nonhealing venous leg ulceration accelerated the healing process. Other studies demonstrated the ability of Propolis phenolic acids and vanillin to penetrate into skin epidermis and dermis and thus to contribute to skin protection from free radicals formed under UV and premature skin aging. Also Brazilian green Propolis which contains biocellulose membranes demonstrates antimicrobial activity and wound healing properties and as such is a promising biomaterial for skin wound healing. From another paper it is clear that Propolis accelerates chondroitin/dermatan sulfates structure modification responsible for binding growth factors which play a crucial role in the tissue repair. Use of Propolis in dentistry is the topic of another group of articles. The exposure of Streptococcus mutans and Lactobacilli isolated from saliva to ethanol extract of Polish Propolis showed its antibacterial effect. Brazilian green Propolis demonstrated a similar effect to miconazole in the treatment of Candida-associated denture stomatitis. A toothpaste containing Propolis was found to be effective in improving oral health and treatment of gingivitis caused by dental plaque. A good effect on the health of oral cavity was also demonstrated when studying the application of toothpaste containing ethanol extract of Brazilian Propolis. EEP had a positive influence on hygiene, gingival condition, and oral microflora in patients with cleft lip and palate treated with fixed orthodontic appliances. Propolis was shown to have antidepressant-like properties, antimicrobial activity, cytotoxic activity, and protective effect against liver damage with cholestasis. Polyphenols that are contained in Propolis possess immunomodulatory, chemopreventive, and antitumor effects. They exert their chemopreventive effect by multiple molecular mechanisms on apoptosis signaling pathways in cancer cells. EEP and polyphenols isolated from Propolis have been shown to sensitize cancer cells to TRAIL-induced apoptosis. We envision that this special issue will arise more interest in Propolis and more interesting investigations will be conducted.
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Propolis: is there a potential for the development of new drugs?
Journal of Ethnopharmacology, 2011Co-Authors: José Maurício Sforcin, Vassya BankovaAbstract:Introduction: Propolis has plenty of biological and pharmacological properties and its mechanisms of action have been widely investigated in the last years, using different experimental models in vitro and in vivo. Researchers have been interested in the investigation of isolated compounds responsible for Propolis action; however, there is lack of clinical research on the effects of Propolis. Strategy and objectives: Since Propolis-containing products have been marketed and humans have used Propolis for different purposes, the goal of this review is to discuss the potential of Propolis for the development of new drugs, by comparing data from the literature that suggest candidate areas for the establishment of drugs against tumors, infections, allergy, diabetes, ulcers and with immunomodulatory action. Conclusions: The efficacy of Propolis in different protocols in vitro and in vivo suggests its therapeutic properties, but before establishing a strategy using this bee product, it is necessary to study: (a) the chemical nature of the Propolis sample. (b) Propolis efficacy should be compared to well-established parameters, e.g. positive or negative controls in the experiments. Moreover, possible interactions between Propolis and other medicines should be investigated in humans as well. (c) Clinical investigation is needed to evaluate Propolis potential in patients or healthy individuals, to understand under which conditions Propolis may promote health. Data point out the importance of this research field not only for the readers and researchers in the scientific community waiting for further clarification on the potential of Propolis but also for the pharmaceutical industry that looks for new drugs. © 2010 Elsevier Ireland Ltd. All rights reserved.
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recent trends and important developments in Propolis research
Evidence-based Complementary and Alternative Medicine, 2005Co-Authors: Vassya BankovaAbstract:The newest developments in Propolis pharmacological research are summarized. The problem regarding biological studies, caused by the chemical variability of Propolis, is discussed. The most important trends and developments in recent Propolis research are outlined: biological studies performed with chemically characterized samples, bioassay-guided studies of active principles and comparative biological studies of Propolis of different origin and chemical composition. These types of studies are extremely valuable with respect to Propolis standardization and practical applications in therapy. They will allow scientists to connect a particular chemical Propolis type to a specific type of biological activity and formulate recommendations for practitioners.
Boryana Trusheva - One of the best experts on this subject based on the ideXlab platform.
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Propolis volatile compounds: chemical diversity and biological activity: a review
Chemistry Central Journal, 2014Co-Authors: Vassya Bankova, Milena Popova, Boryana TrushevaAbstract:Propolis is a sticky material collected by bees from plants, and used in the hive as building material and defensive substance. It has been popular as a remedy in Europe since ancient times. Nowadays, Propolis use in over-the-counter preparations, “bio”-cosmetics and functional foods, etc., increases. Volatile compounds are found in low concentrations in Propolis, but their aroma and significant biological activity make them important for Propolis characterisation. Propolis is a plant-derived product: its chemical composition depends on the local flora at the site of collection, thus it offers a significant chemical diversity. The role of Propolis volatiles in identification of its plant origin is discussed. The available data about chemical composition of Propolis volatiles from different geographic regions are reviewed, demonstrating significant chemical variability. The contribution of volatiles and their constituents to the biological activities of Propolis is considered. Future perspectives in research on Propolis volatiles are outlined, especially in studying activities other than antimicrobial.
Mochamad Moedjiono - One of the best experts on this subject based on the ideXlab platform.
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The cytotoxicity test of calcium hydroxide, Propolis, and calcium hydroxide-Propolis combination in human pulp fibroblast.
Journal of Advanced Pharmaceutical Technology & Research, 2020Co-Authors: Ira Widjiastuti, Mieke Kusuma Dewi, Edhi Arief Prasetyo, Nirawati Pribadi, Mochamad MoedjionoAbstract:Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp-capping but still has a high failure rate. Thus, an alternative material is needed, one of which is Propolis. The combination of Ca(OH)2Propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, Propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16-year-old patients, were divided into seven groups: Group 1 (10 μg Ca(OH)2); Group 2 (10 μg Propolis); Group 3 (15 μg Propolis); Group 4 (20 μg Propolis); Group 5 (Ca(OH)2-Propolis 1:1); Group 6 (Ca(OH)2-Propolis 1:1.5); and Group 7 (calcium hydroxide-Propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene's test, one-way analysis of variance, and Tukey-honestly significant difference (P
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The cytotoxicity test of calcium hydroxide, Propolis, and calcium hydroxide‑Propolis combination in human pulp fibroblast
Wolters Kluwer - Medknow, 2020Co-Authors: Ira Widjiastuti, Mieke Kusuma Dewi, Edhi Arief Prasetyo, Nirawati Pribadi, Mochamad MoedjionoAbstract:Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp‑capping but still has a high failure rate. Thus, an alternative material is needed, one of which is Propolis. The combination of Ca(OH)2 Propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, Propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16‑year‑old patients, were divided into seven groups: Group 1 (10 µg Ca(OH)2); Group 2 (10 µg Propolis); Group 3 (15 µg Propolis); Group 4 (20 µg Propolis); Group 5 (Ca(OH)2‑Propolis 1:1); Group 6 (Ca(OH)2‑Propolis 1:1.5); and Group 7 (calcium hydroxide‑Propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene’s test, one‑way analysis of variance, and Tukey‑honestly significant difference (P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH)2 and Propolis combination is greater than the application of Ca(OH)2 or Propolis with significant different between groups (P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH)2‑Propolis combination is greater than that of the application of Ca(OH)2 and Propolis alone
Maria Cristina Marcucci - One of the best experts on this subject based on the ideXlab platform.
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antibacterial activity of honey and Propolis from apis mellifera and tetragonisca angustula against staphylococcus aureus
Journal of Applied Microbiology, 2003Co-Authors: P L Miorin, N C Levy, Angela Ramalho Custodio, Walter A Bretz, Maria Cristina MarcucciAbstract:Aims: The antibacterial activity against Staphylococcus aureus of honey and Propolis produced by Apis mellifera and Tetragonisca angustula was evaluated. Secondary aims included the study of the chemical composition of Propolis and honey samples and its relationship with antibacterial activity against S. aureus. Methods and Results: The antibacterial activity of honey and Propolis was determined by the method of macrodilution. The minimum inhibitory concentration (MICs) of A. mellifera honey ranged from 126·23 to 185·70 mg ml−1 and of T. angustula from 142·87 to 214·33 mg ml−1. For Propolis, the MIC ranged from 0·36 to 3·65 mg ml−1 (A. mellifera) and from 0·44 to 2·01 mg ml−1 (T. angustula). Honey and Propolis were evaluated by high-performance liquid chromatography. Some typical compounds of Brazilian Propolis were also identified in honey samples. Principal component analysis revealed that the chemical composition of honey and Propolis samples was distinct based on the geographical location of the samples. Conclusions: Propolis samples had higher antibacterial activity against S. aureus when compared with honey. However, both Propolis and honey samples had antibacterial against S. aureus. Significance and Impact of the Study: These antimicrobial properties would warrant further studies on the clinical applications of Propolis and honey against S. aureus.
Ira Widjiastuti - One of the best experts on this subject based on the ideXlab platform.
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The cytotoxicity test of calcium hydroxide, Propolis, and calcium hydroxide-Propolis combination in human pulp fibroblast.
Journal of Advanced Pharmaceutical Technology & Research, 2020Co-Authors: Ira Widjiastuti, Mieke Kusuma Dewi, Edhi Arief Prasetyo, Nirawati Pribadi, Mochamad MoedjionoAbstract:Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp-capping but still has a high failure rate. Thus, an alternative material is needed, one of which is Propolis. The combination of Ca(OH)2Propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, Propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16-year-old patients, were divided into seven groups: Group 1 (10 μg Ca(OH)2); Group 2 (10 μg Propolis); Group 3 (15 μg Propolis); Group 4 (20 μg Propolis); Group 5 (Ca(OH)2-Propolis 1:1); Group 6 (Ca(OH)2-Propolis 1:1.5); and Group 7 (calcium hydroxide-Propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene's test, one-way analysis of variance, and Tukey-honestly significant difference (P
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The cytotoxicity test of calcium hydroxide, Propolis, and calcium hydroxide‑Propolis combination in human pulp fibroblast
Wolters Kluwer - Medknow, 2020Co-Authors: Ira Widjiastuti, Mieke Kusuma Dewi, Edhi Arief Prasetyo, Nirawati Pribadi, Mochamad MoedjionoAbstract:Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp‑capping but still has a high failure rate. Thus, an alternative material is needed, one of which is Propolis. The combination of Ca(OH)2 Propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, Propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16‑year‑old patients, were divided into seven groups: Group 1 (10 µg Ca(OH)2); Group 2 (10 µg Propolis); Group 3 (15 µg Propolis); Group 4 (20 µg Propolis); Group 5 (Ca(OH)2‑Propolis 1:1); Group 6 (Ca(OH)2‑Propolis 1:1.5); and Group 7 (calcium hydroxide‑Propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene’s test, one‑way analysis of variance, and Tukey‑honestly significant difference (P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH)2 and Propolis combination is greater than the application of Ca(OH)2 or Propolis with significant different between groups (P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH)2‑Propolis combination is greater than that of the application of Ca(OH)2 and Propolis alone
