The Experts below are selected from a list of 4314 Experts worldwide ranked by ideXlab platform
Vera Jonáková - One of the best experts on this subject based on the ideXlab platform.
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Isolation of non-heparin-binding and heparin-binding proteins of boar Prostate.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2002Co-Authors: Pavla Manásková, Jirí Liberda, Marie Tichá, Vera JonákováAbstract:Proteins of boar Prostate Secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding (H) and heparin-binding (H+) protein fractions. H- and H+ fractions were then subjected to RP HPLC. Elution profiles of H-and H+ fractions of Prostate Secretion were compared with those of seminal plasma and the amounts of corresponding proteins were compared. Besides, the isolated proteins were characterized by SDS-PAGE. In the H- fraction of Prostate Secretion, PSP I and PSP II spermadhesins and in the H+ fraction AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the contrary, beta-microseminoprotein was identified in abundant amounts both in H- and H+ fractions of boar Prostate Secretion. AQN 2 and AWN 1 spermadhesins were proved by their antibodies. Some seminal plasma proteins originating mainly in seminal vesicles could also be secreted by the prostatic gland. beta-Microseminoprotein was found to be produced mainly by the Prostate.
Pavla Manásková - One of the best experts on this subject based on the ideXlab platform.
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Isolation of non-heparin-binding and heparin-binding proteins of boar Prostate.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2002Co-Authors: Pavla Manásková, Jirí Liberda, Marie Tichá, Vera JonákováAbstract:Proteins of boar Prostate Secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding (H) and heparin-binding (H+) protein fractions. H- and H+ fractions were then subjected to RP HPLC. Elution profiles of H-and H+ fractions of Prostate Secretion were compared with those of seminal plasma and the amounts of corresponding proteins were compared. Besides, the isolated proteins were characterized by SDS-PAGE. In the H- fraction of Prostate Secretion, PSP I and PSP II spermadhesins and in the H+ fraction AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the contrary, beta-microseminoprotein was identified in abundant amounts both in H- and H+ fractions of boar Prostate Secretion. AQN 2 and AWN 1 spermadhesins were proved by their antibodies. Some seminal plasma proteins originating mainly in seminal vesicles could also be secreted by the prostatic gland. beta-Microseminoprotein was found to be produced mainly by the Prostate.
Camilla Valtonenandre - One of the best experts on this subject based on the ideXlab platform.
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beta microseminoprotein in serum correlates with the levels in seminal plasma of young healthy males
Journal of Andrology, 2008Co-Authors: Camilla Valtonenandre, Per Fernlund, Charlotta Sävblom, Aleksander Giwercman, Hans Lilja, Åke LundwallAbstract:Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the Prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the Prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and Prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the Prostate Secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of Prostate Secretion, despite the contribution from extra prostatic tissues. (Less)
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structure evolution and expression of proteins in the male reproductive tract studies on semenogelins beta microseminoprotein and pc3 secreted microprotein
(2007), 2007Co-Authors: Camilla ValtonenandreAbstract:Human semen has several important constituents, including the Prostate secreted proteins beta-microseminoprotein (MSP), Prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), and the seminal-vesicle-derived coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2). The research underlying this thesis explored the evolution, expression and structure of seminal plasma proteins to provide further knowledge in the area of reproduction, particularly regarding MSP and the semenogelins. Several of the studies focused on two species of New World monkeys, that represent evolutionary intermediates between the rodents and humans, and work was also done to characterize a human MSP homologue, and obtain physiological data on human MSP. The results show that the cotton-top tamarin carries five genes for MSP, one of which exhibits transcriptional activity, and two are nonfunctional pseudogenes. Moreover, the closely related common marmoset has several MSP genes and at least three different forms of MSP in the ejaculate, which also contains semenogelins, but not PAP and PSA. In the marmoset, the genes for both SEMG1 and SEMG2 are transcribed in the seminal vesicles, and they are very similar due to gene conversion. Marmoset SEMG1 has two allelic variants, which differ by one 60-amino-acid repeat. The studies also revealed the existence of a previously unknown and highly conserved human gene with distant homology to the MSP gene. The protein encoded by this gene is designated PC3-secreted microprotein (PSMP), because it is expressed and secreted by the Prostate cancer cell line PC-3, and it can also be detected in benign and malignant Prostate tissues. An immunoassay for MSP was developed and used to measure the protein in serum and seminal plasma from young men. Correlations were found between the levels of MSP in seminal plasma and serum, and also between the concentrations of MSP, zinc and PSA in seminal plasma. Accordingly, we suggest that MSP in serum and semen can serve as a valid marker of Prostate Secretion. (Less)
Åke Lundwall - One of the best experts on this subject based on the ideXlab platform.
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beta microseminoprotein in serum correlates with the levels in seminal plasma of young healthy males
Journal of Andrology, 2008Co-Authors: Camilla Valtonenandre, Per Fernlund, Charlotta Sävblom, Aleksander Giwercman, Hans Lilja, Åke LundwallAbstract:Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the Prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the Prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and Prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the Prostate Secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of Prostate Secretion, despite the contribution from extra prostatic tissues. (Less)
Ekaterina Kulchavenya - One of the best experts on this subject based on the ideXlab platform.
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Spectrum of urogenital tuberculosis
Journal of Infection and Chemotherapy, 2013Co-Authors: Ekaterina Kulchavenya, Irina Zhukova, Denis KholtobinAbstract:Urogenital tuberculosis (UGTB) plays an important role because its complications may be fatal, it significantly reduces quality of life, and it is often associated with AIDS. Diagnosis of UGTB is often delayed. We analyzed 131 case histories of UGTB patients from the years 2009–2011. Gender, age, and the clinical form and main features of the disease were taken into account. The most common form was kidney tuberculosis (74.8 %). Isolated kidney tuberculosis (KTB) more often occurs in women: 56.8 %. Patients of middle and old age more often showed the stage of cavernous KTB; younger patients had smaller forms. Among all cases, an asymptomatic course was seen in 12.2 % and, among cases of KTB, in 15.9 %. Every third patient complained of flank pain and dysuria (35.2 % and 39.8 %, respectively); 17 % presented with toxicity symptoms, 9.1 % with renal colic, and 7.9 % with gross hematuria. Mycobacterium tuberculosis (MTB) in urine was found in 31.8 % of cases in all levels of isolated KTB. UGTB has no specific symptom; even sterile pyuria occurs only in 25 %. The acute onset of tuberculous orchiepididymitis was seen in 35.7 % of patients, hemospermia in 7.1 %, and dysuria in 35.7 %. The most common complaints for Prostate tuberculosis were perineal pain (31.6 %), dysuria (also 31.6 %), and hemospermia (26.3 %). MTB in Prostate Secretion/ejaculate was revealed in 10.5 % of this group. All urogenital tract infections should be suspected as UGTB in patients who are living in a region with a high incidence rate, who have had contact with tuberculosis infection, and who have a recurrence of the disease that is resistant to standard therapy.
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Male genital tuberculosis in Siberians
World Journal of Urology, 2006Co-Authors: Ekaterina Kulchavenya, Victor KhomyakovAbstract:To study the epidemiology and the clinical picture of male genital tuberculosis in Siberia, Russia. Five hundred and fourteen patients with genitourinary tuberculosis were enrolled in the study: 414 had kidney tuberculosis only, 100 had genital involvement. The clinical picture and structure of genital tuberculosis were investigated: 42 had tuberculosis of scrotal organs and 58 had tuberculosis of the Prostate. Urinary cultures, urinalysis, three-glass test, and investigation of the Prostate Secretion, Mycobacteria culture, and susceptibility testing were performed in all 514 patients. 33.6% of all patients earlier suffered from pulmonary or extrapulmonary tuberculosis and were successfully cured. In 61.9% nephrotuberculosis was diagnosed alongside with an orchiepididymitis. In 30.9% of patients bilateral epididymorchitis was diagnosed. Mycobacteriuria was present in 38.1%. Scrotal fistula was found in 11.9%. In 66.7% the symptoms appeared acutely. Half of the patients with Prostate tuberculosis complained of dysuria, 23 (39.6%) had perineal pain, 34 (58.6%) had flank pain. Leucocytes in urine were present in 49 (84.5%) patients, and in prostatic Secretion in 45 (77.6%) patients. Erythrocytes in urine were present in 31 (53.4%) patients, and in prostatic Secretions in 17 (29.3%) patients. Male genital tuberculosis has no specific pathognomonic signs. Using a special algorithm for the management of patients with prostatitis or epididymitis is recommended.
