The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Xavier Druart - One of the best experts on this subject based on the ideXlab platform.
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Seminal Plasma proteins as markers of sperm fertility.
Theriogenology, 2019Co-Authors: Xavier Druart, J.p. Rickard, Guillaume Tsikis, Simon P. De GraafAbstract:Abstract During ejaculation and deposition in the female genital tract, spermatozoa are exposed to Seminal Plasma, a mix of secretions primarily from the accessory sex glands. Proteins, which make up the largest contribution to Seminal Plasma by weight, have been the focus of much interest, in particular the identification of specific proteins both in the Plasma and/or found bound to the sperm surface post ejaculation. Global proteomic studies of Seminal Plasma originating from a range of species over the last 15 years have revealed their hitherto unknown diversity and complexity. Seminal Plasma is generally known to aid sperm survival and fertility in a range of species and studies have begun to reveal its link with sperm function and identification, as markers of fertility. This review summarises recent data on proteins found on the sperm surface that originate from Seminal Plasma and have subsequently been shown to correlate with fertility, with a focus on the pig.
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Seminal Plasma proteins as markers of sperm fertility
Theriogenology, 2019Co-Authors: Xavier Druart, J.p. Rickard, Guillaume Tsikis, Simon P. De GraafAbstract:During ejaculation and deposition in the female genital tract, spermatozoa are exposed to Seminal Plasma, a mix of secretions primarily from the accessory sex glands. Proteins, which make up the largest contribution to Seminal Plasma by weight, have been the focus of much interest, in particular the identification of specific proteins both in the Plasma and/or found bound to the sperm surface post ejaculation. Global proteomic studies of Seminal Plasma originating from a range of species over the last 15 years have revealed their hitherto unknown diversity and complexity. Seminal Plasma is generally known to aid sperm survival and fertility in a range of species and studies have begun to reveal its link with sperm function and identification, as markers of fertility. This review summarises recent data on proteins found on the sperm surface that originate from Seminal Plasma and have subsequently been shown to correlate with fertility, with a focus on the pig. (C) 2019 Published by Elsevier Inc.
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Seminal Plasma proteomes and sperm fertility.
Animal Reproduction Science, 2018Co-Authors: Xavier Druart, Simon P. De GraafAbstract:Abstract During ejaculation, the spermatozoa are transported by the Seminal Plasma, a fluid resulting from secretions originating mainly from the prostate and the Seminal vesicles in mammals. The interaction of the Seminal Plasma with spermatozoa induces binding of Seminal proteins onto the sperm surface and membrane remodeling potentially impacting the sperm transport, survival and fertilizing ability in the female genital tract. The Seminal Plasma also contains peptides and proteins involved in the inflammatory and immune response of the female tract. Therefore the Seminal Plasma proteome has been investigated in a large range of taxa, including mammals, birds, fishes and insect species. The association of the Seminal Plasma with semen preservation or fertility identified proteic markers of Seminal Plasma function in domestic species. This review summarizes the current knowledge in Seminal Plasma proteomes and proteic markers of sperm preservation in animal species.
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Proteomic characterization and cross species comparison of mammalian Seminal Plasma
Journal of Proteomics, 2013Co-Authors: Xavier Druart, C.m. Kershaw-young, Guillaume Tsikis, J.p. Rickard, S. Mactier, Philippa Kohnke, R. Bathgate, Z. Gibb, B. Crossett, Valérie LabasAbstract:Seminal Plasma contains a large protein component which has been implicated in the function, transit and survival of spermatozoa within the female reproductive tract. However, the identity of the majority of these proteins remains unknown and a direct comparison between the major domestic mammalian species has yet to be made. As such, the present study characterized and compared the Seminal Plasma proteomes of cattle, horse, sheep, pig, goat, camel and alpaca. GeLC-MS/MS and shotgun proteomic analysis by 2D-LC-MS/MS identified a total of 302 proteins in the Seminal Plasma of the chosen mammalian species. Nucleobindin 1 and RSVP14, a member of the BSP (binder of sperm protein) family, were identified in all species. Beta nerve growth factor (bNGF), previously identified as an ovulation inducing factor in alpacas and llamas, was identified in this study in alpaca and camel (induced ovulators), cattle, sheep and horse (spontaneous ovulators) Seminal Plasma. These findings indicate that while the mammalian species studied have common ancestry as ungulates, their Seminal Plasma is divergent in protein composition, which may explain variation in reproductive capacity and function. The identification of major specific proteins within Seminal Plasma facilitates future investigation of the role of each protein in mammalian reproduction. BIOLOGICAL SIGNIFICANCE: This proteomic study is the first study to compare the protein composition of Seminal Plasma from seven mammalian species including two camelid species. Beta nerve growth factor, previously described as the ovulation inducing factor in camelids is shown to be the major protein in alpaca and camel Seminal Plasma and also present in small amounts in bull, ram, and horse Seminal Plasma.
Simon P. De Graaf - One of the best experts on this subject based on the ideXlab platform.
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Seminal Plasma proteins as markers of sperm fertility.
Theriogenology, 2019Co-Authors: Xavier Druart, J.p. Rickard, Guillaume Tsikis, Simon P. De GraafAbstract:Abstract During ejaculation and deposition in the female genital tract, spermatozoa are exposed to Seminal Plasma, a mix of secretions primarily from the accessory sex glands. Proteins, which make up the largest contribution to Seminal Plasma by weight, have been the focus of much interest, in particular the identification of specific proteins both in the Plasma and/or found bound to the sperm surface post ejaculation. Global proteomic studies of Seminal Plasma originating from a range of species over the last 15 years have revealed their hitherto unknown diversity and complexity. Seminal Plasma is generally known to aid sperm survival and fertility in a range of species and studies have begun to reveal its link with sperm function and identification, as markers of fertility. This review summarises recent data on proteins found on the sperm surface that originate from Seminal Plasma and have subsequently been shown to correlate with fertility, with a focus on the pig.
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Seminal Plasma proteins as markers of sperm fertility
Theriogenology, 2019Co-Authors: Xavier Druart, J.p. Rickard, Guillaume Tsikis, Simon P. De GraafAbstract:During ejaculation and deposition in the female genital tract, spermatozoa are exposed to Seminal Plasma, a mix of secretions primarily from the accessory sex glands. Proteins, which make up the largest contribution to Seminal Plasma by weight, have been the focus of much interest, in particular the identification of specific proteins both in the Plasma and/or found bound to the sperm surface post ejaculation. Global proteomic studies of Seminal Plasma originating from a range of species over the last 15 years have revealed their hitherto unknown diversity and complexity. Seminal Plasma is generally known to aid sperm survival and fertility in a range of species and studies have begun to reveal its link with sperm function and identification, as markers of fertility. This review summarises recent data on proteins found on the sperm surface that originate from Seminal Plasma and have subsequently been shown to correlate with fertility, with a focus on the pig. (C) 2019 Published by Elsevier Inc.
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Seminal Plasma proteomes and sperm fertility.
Animal Reproduction Science, 2018Co-Authors: Xavier Druart, Simon P. De GraafAbstract:Abstract During ejaculation, the spermatozoa are transported by the Seminal Plasma, a fluid resulting from secretions originating mainly from the prostate and the Seminal vesicles in mammals. The interaction of the Seminal Plasma with spermatozoa induces binding of Seminal proteins onto the sperm surface and membrane remodeling potentially impacting the sperm transport, survival and fertilizing ability in the female genital tract. The Seminal Plasma also contains peptides and proteins involved in the inflammatory and immune response of the female tract. Therefore the Seminal Plasma proteome has been investigated in a large range of taxa, including mammals, birds, fishes and insect species. The association of the Seminal Plasma with semen preservation or fertility identified proteic markers of Seminal Plasma function in domestic species. This review summarizes the current knowledge in Seminal Plasma proteomes and proteic markers of sperm preservation in animal species.
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Application of Seminal Plasma in sex-sorting and sperm cryopreservation.
Theriogenology, 2008Co-Authors: Simon P. De Graaf, T. Leahy, J.i. Marti, Gareth Evans, W.m.c. MaxwellAbstract:Substantial dilution of boar semen during processing decreased the concentration of Seminal Plasma, perhaps contributing to the decline in sperm quality after cryopreservation and sex-sorting. Results of replacing Seminal Plasma in investigations from many laboratories have been contradictory. Results and discussion here suggest that whereas membrane status can be influenced by Seminal Plasma, the action of its various components, both positive and negative, is determined in part by the membrane status of the spermatozoa to which it is being exposed. Although progress has been made in identifying components of Seminal Plasma responsible for its protective effect (notably PSP-I/II spermadhesin for sex-sorted boar spermatozoa), little is known (in any species) regarding how external factors may influence their levels, and their functionality, in Seminal Plasma. It is noteworthy that Seminal Plasma is beneficial to post-thaw quality of sex-sorted ram spermatozoa only when added before freezing, not after thawing. Therefore, the action of Seminal Plasma and its components is dependent on sperm-related factors, in particular the type of processing to which they have been previously exposed. Further research is needed to unravel these biological complexities, and then characterise and synthesise useful proteins within Seminal Plasma.
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Seminal Plasma effects on sperm handling and female fertility
Reproduction in domestic ruminants VI. Proceedings of the Seventh International Symposium on Reproduction in Domestic Ruminants Wellington New Zealand, 2007Co-Authors: W.m.c. Maxwell, Simon P. De Graaf, Elhajj R Ghaoui, Gareth EvansAbstract:The components of ruminant Seminal Plasma and their influence on the fertility of spermatozoa are reviewed. Seminal Plasma can both inhibit and stimulate sperm function and fertility through the multifunctional actions of organic and inorganic components. These effects are now better understood because the composition of the Seminal Plasma, including its protein content and that of other structures, specifically membrane vesicles, has been clarified. Spermatozoa gain motility and fertilizing capacity as they transit the epididymis under the influence of factors produced by that organ. At ejaculation, inhibitory (termed "decapacitation") factors, sourced from the accessory sex glands, bind to the sperm surface. The major proteins isolated and characterised in ram Seminal Plasma, whose specific functions are yet to be determined, originate from the vesicular gland and comprise a spermadhesin together with proteins with fibronectin-II domains. In vitro handling of spermatozoa in preparation for artificial insemination (AI), involving processes such as dilution, cooling, freezing, re-warming and sperm sexing by flow cytometric sorting, can remove Seminal Plasma and may modify the proteins bound to the sperm surface. This destabilises the membranes and may pre-capacitate the spermatozoa, shortening their fertilizing lifespan. These changes may be reversible by Seminal Plasma fractions but responses differ depending on the type of sperm pre-treatment. Fertility after AI of ruminant semen may be improved if the role of Seminal Plasma proteins and their effect, if added individually or in combination to spermatozoa at different stages of preservation, or other manipulations such as flow cytometric sorting, can be determined.
W.m.c. Maxwell - One of the best experts on this subject based on the ideXlab platform.
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The effect of Seminal Plasma on alpaca sperm function.
Theriogenology, 2011Co-Authors: C.m. Kershaw-young, W.m.c. MaxwellAbstract:In order to advance the development of assisted reproductive technologies in alpacas and other Camelids, the objective of this study was to explore the role of Seminal Plasma concentration on motility and functional integrity of alpaca sperm. Sixteen male alpacas > 3 y of age were used. In Experiment 1, epididymal sperm were incubated for 0 to 6 h in 0, 10, 25, 50, or 100% Seminal Plasma and motility was assessed. In Experiment 2, epididymal sperm were incubated in 0, 10, or 100% Seminal Plasma for 3 h and motility, acrosome integrity and DNA integrity were assessed. In Experiment 3, ejaculated sperm were incubated in 10, 25, 50, or 100% Seminal Plasma for 0 to 6 h and motility assessed. In Experiment 4, ejaculated sperm were incubated in 10 or 100% Seminal Plasma for 3 h and motility, acrosome integrity, DNA integrity, and viability were assessed. Epididymal and ejaculated sperm maintained motility longer when incubated in the presence of 10% Seminal Plasma compared to 0, 25, 50, or 100% Seminal Plasma (P < 0.001). The mean ± SEM percentage of epididymal sperm with intact acrosomes was less (P < 0.001) in samples incubated in 0% Seminal Plasma (39.4 ± 3.73) compared to 10% (75.3 ± 1.20) or 100% (77.4 ± 0.90) within 1 h after incubation. However, DNA integrity of ejaculated and epididymal sperm was not significantly affected by Seminal Plasma concentration. The mean viability of ejaculated sperm was reduced in the presence of 100 (12.7 ± 2.33) compared to 10% (36.2 ± 4.68) Seminal Plasma (P < 0.001) within 1 h of incubation. We concluded that alpaca semen should be diluted to a final concentration of 10% Seminal Plasma to prolong motility, preserve acrosome integrity, and maintain viability of sperm.
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Application of Seminal Plasma in sex-sorting and sperm cryopreservation.
Theriogenology, 2008Co-Authors: Simon P. De Graaf, T. Leahy, J.i. Marti, Gareth Evans, W.m.c. MaxwellAbstract:Substantial dilution of boar semen during processing decreased the concentration of Seminal Plasma, perhaps contributing to the decline in sperm quality after cryopreservation and sex-sorting. Results of replacing Seminal Plasma in investigations from many laboratories have been contradictory. Results and discussion here suggest that whereas membrane status can be influenced by Seminal Plasma, the action of its various components, both positive and negative, is determined in part by the membrane status of the spermatozoa to which it is being exposed. Although progress has been made in identifying components of Seminal Plasma responsible for its protective effect (notably PSP-I/II spermadhesin for sex-sorted boar spermatozoa), little is known (in any species) regarding how external factors may influence their levels, and their functionality, in Seminal Plasma. It is noteworthy that Seminal Plasma is beneficial to post-thaw quality of sex-sorted ram spermatozoa only when added before freezing, not after thawing. Therefore, the action of Seminal Plasma and its components is dependent on sperm-related factors, in particular the type of processing to which they have been previously exposed. Further research is needed to unravel these biological complexities, and then characterise and synthesise useful proteins within Seminal Plasma.
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Seminal Plasma effects on sperm handling and female fertility
Reproduction in domestic ruminants VI. Proceedings of the Seventh International Symposium on Reproduction in Domestic Ruminants Wellington New Zealand, 2007Co-Authors: W.m.c. Maxwell, Simon P. De Graaf, Elhajj R Ghaoui, Gareth EvansAbstract:The components of ruminant Seminal Plasma and their influence on the fertility of spermatozoa are reviewed. Seminal Plasma can both inhibit and stimulate sperm function and fertility through the multifunctional actions of organic and inorganic components. These effects are now better understood because the composition of the Seminal Plasma, including its protein content and that of other structures, specifically membrane vesicles, has been clarified. Spermatozoa gain motility and fertilizing capacity as they transit the epididymis under the influence of factors produced by that organ. At ejaculation, inhibitory (termed "decapacitation") factors, sourced from the accessory sex glands, bind to the sperm surface. The major proteins isolated and characterised in ram Seminal Plasma, whose specific functions are yet to be determined, originate from the vesicular gland and comprise a spermadhesin together with proteins with fibronectin-II domains. In vitro handling of spermatozoa in preparation for artificial insemination (AI), involving processes such as dilution, cooling, freezing, re-warming and sperm sexing by flow cytometric sorting, can remove Seminal Plasma and may modify the proteins bound to the sperm surface. This destabilises the membranes and may pre-capacitate the spermatozoa, shortening their fertilizing lifespan. These changes may be reversible by Seminal Plasma fractions but responses differ depending on the type of sperm pre-treatment. Fertility after AI of ruminant semen may be improved if the role of Seminal Plasma proteins and their effect, if added individually or in combination to spermatozoa at different stages of preservation, or other manipulations such as flow cytometric sorting, can be determined.
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The origin of membrane vesicles in ram Seminal Plasma.
Reproduction in domestic animals = Zuchthygiene, 2006Co-Authors: R. El-hajj Ghaoui, Gareth Evans, Peter C. Thomson, W.m.c. MaxwellAbstract:Contents The hypothesis tested in this study was that the membrane vesicles present in ram Seminal Plasma are of testicular origin, rather than being secreted by the accessory sex glands as has been previously reported for a number of species. Membrane vesicles were present in cellular extracts from reproductive organs and accessory sex glands of six rams, and in the Seminal Plasma of a further eight rams. When four of the latter rams were subjected to vasectomy, to isolate ejaculate contents to only the secretions of the accessory sex glands, the vesicles were largely eliminated from their ejaculates, while vesicles were still present in the ejaculates of the four control rams. The constituents of the cytoplasmic droplets and membrane vesicles derived from the Seminal Plasma were compared by transmission electron microscopy (TEM). Vesicles present in the cytoplasmic droplets were similar in morphology but smaller on average than those in the Seminal Plasma. It was concluded that the membrane vesicles in ram Seminal Plasma originate from either the cytoplasmic droplets, or a combination of vesicles from the droplets and the epididymis.
Woelders Henri - One of the best experts on this subject based on the ideXlab platform.
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Seminal Plasma amino acid profile in different breeds of chicken : Role of Seminal Plasma on sperm cryoresistance
2019Co-Authors: Santiago-moreno Juliá, Ernal Erenice, Pérez-cerezales Serafí, Castaño Cristina, Toledano-díaz Adolfo, Esteso, Milagros C., Gutiérrez-adá Alfonso, López-sebastiá Antonio, Gil, María G., Woelders HenriAbstract:Seminal Plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of Seminal Plasma in 12 Spanish chicken breeds and to investigate the role of Seminal Plasma on cryoresistance of rooster sperm. To investigate the role of Seminal Plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of Seminal Plasma. Glutamic acid was the most abundant free amino acid in Seminal Plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with Seminal Plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between Seminal Plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of Seminal Plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between Seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of Seminal Plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific Seminal Plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of Seminal Plasma decreases the variability of the results and DNA fragmentation damages.
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Seminal Plasma amino acid profile in different breeds of chicken: Role of Seminal Plasma on sperm cryoresistance
2019Co-Authors: Ernal Erenice, Pérez-cerezales Serafí, Castaño Cristina, Toledano-díaz Adolfo, Esteso, Milagros C., Gutiérrez-adá Alfonso, López-sebastiá Antonio, Gil, María G., Woelders Henri, Lesbois ElisabethAbstract:Seminal Plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of Seminal Plasma in 12 Spanish chicken breeds and to investigate the role of Seminal Plasma on cryoresistance of rooster sperm. To investigate the role of Seminal Plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of Seminal Plasma. Glutamic acid was the most abundant free amino acid in Seminal Plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P
Ernal Erenice - One of the best experts on this subject based on the ideXlab platform.
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Seminal Plasma amino acid profile in different breeds of chicken : Role of Seminal Plasma on sperm cryoresistance
2019Co-Authors: Santiago-moreno Juliá, Ernal Erenice, Pérez-cerezales Serafí, Castaño Cristina, Toledano-díaz Adolfo, Esteso, Milagros C., Gutiérrez-adá Alfonso, López-sebastiá Antonio, Gil, María G., Woelders HenriAbstract:Seminal Plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of Seminal Plasma in 12 Spanish chicken breeds and to investigate the role of Seminal Plasma on cryoresistance of rooster sperm. To investigate the role of Seminal Plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of Seminal Plasma. Glutamic acid was the most abundant free amino acid in Seminal Plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with Seminal Plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between Seminal Plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of Seminal Plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between Seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of Seminal Plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific Seminal Plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of Seminal Plasma decreases the variability of the results and DNA fragmentation damages.
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Seminal Plasma amino acid profile in different breeds of chicken: Role of Seminal Plasma on sperm cryoresistance
2019Co-Authors: Ernal Erenice, Pérez-cerezales Serafí, Castaño Cristina, Toledano-díaz Adolfo, Esteso, Milagros C., Gutiérrez-adá Alfonso, López-sebastiá Antonio, Gil, María G., Woelders Henri, Lesbois ElisabethAbstract:Seminal Plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of Seminal Plasma in 12 Spanish chicken breeds and to investigate the role of Seminal Plasma on cryoresistance of rooster sperm. To investigate the role of Seminal Plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of Seminal Plasma. Glutamic acid was the most abundant free amino acid in Seminal Plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P
