The Experts below are selected from a list of 33048 Experts worldwide ranked by ideXlab platform
Laura Cancedda - One of the best experts on this subject based on the ideXlab platform.
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Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe
Nature Protocols, 2016Co-Authors: Joanna Szczurkowska, Andrzej W. Cwetsch, Diego Ghezzi, Gian Michele Ratto, Marco Dal Maschio, Laura CanceddaAbstract:This Protocol is an Extension to: Nat. Protoc. 1 , 1552–1558 (2006); doi:10.1038/nprot.2006.276; published online 9 November 2006 This article describes how to reliably electroporate with DNA plasmids rodent neuronal progenitors of the hippocampus; the motor, prefrontal and visual cortices; and the cerebellum in utero. As a Protocol Extension article, this article describes an adaptation of an existing Protocol and offers additional applications. The earlier Protocol describes how to electroporate mouse embryos using two standard forceps-type electrodes. In the present Protocol, additional electroporation configurations are possible because of the addition of a third electrode alongside the two standard forceps-type electrodes. By adjusting the position and polarity of the three electrodes, the electric field can be directed with great accuracy to different neurogenic areas. Bilateral transfection of brain hemispheres can be achieved after a single electroporation episode. Approximately 75% of electroporated embryos survive to postnatal ages, and depending on the target area, 50–90% express the electroporated vector. The electroporation procedure takes 1 h 35 min. The Protocol is suitable for the preparation of animals for various applications, including histochemistry, behavioral studies, electrophysiology and in vivo imaging. This Protocol Extension describes how to electroporate rodent neuronal progenitors in utero with greater accuracy by using triple-electrode probes.
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Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe
Nature protocols, 2016Co-Authors: Joanna Szczurkowska, Andrzej W. Cwetsch, Marco Dal Maschio, Diego Ghezzi, Gian Michele Ratto, Laura CanceddaAbstract:This Protocol Extension describes how to electroporate rodent neuronal progenitors in utero with greater accuracy by using triple-electrode probes.
Joakim Lundeberg - One of the best experts on this subject based on the ideXlab platform.
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Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays
Nature Protocols, 2018Co-Authors: Stefania Giacomello, Joakim LundebergAbstract:This Protocol Extension describes how to prepare plant tissue to enable Spatial Transcriptomics profiling. Spatial Transcriptomics is achieved through the combination of histological staining of the plant tissue with spatially resolved RNA sequencing. Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This Protocol Extension is an adaptation of a Protocol that describes how to use barcoded oligo-dT microarrays to evaluate spatial global gene expression profiles in mammalian tissue to enable it to be applied to plant material. Here, we explain the required adjustments for preparing and treating plant tissue sections on the array surface, specifically in regard to how to permeabilize and remove the tissue. Once the tissue has been removed, the cDNA–mRNA hybrid that is left on the slide is processed in the same way as cDNA obtained during experiments on mammalian tissue; thus the later stages of the Protocol are not included here, and readers should follow the accompanying Protocol for those. We have previously used our Protocol to generate high-quality sequencing libraries for Arabidopsis thaliana inflorescence, Populus tremula developing and dormant leaf buds, and Picea abies female cones. However, we anticipate that the Protocol can be adapted to other tissue types and species. The entire Protocol for preparing samples and processing libraries can be completed in 3–4 d.
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Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays
Nature protocols, 2018Co-Authors: Stefania Giacomello, Joakim LundebergAbstract:Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This Protocol Extension is an adaptation of a protoc ...
Peter Saint-andre - One of the best experts on this subject based on the ideXlab platform.
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Publishing Available Jingle Sessions
2017Co-Authors: Philipp Hancke, Lance Stout, Peter Saint-andreAbstract:This specification defines an XMPP Protocol Extension that enables an XMPP entity to advertise the fact that it is willing accept a particular Jingle session request. The Protocol is used mainly to inform other entities that a particular file is available for transfer via the Jingle File Transfer Protocol defined in XEP-0234.
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External Service Discovery
2015Co-Authors: Peter Saint-andre, Sean Egan, Marcus Lundblad, Lance StoutAbstract:This document specifies an XMPP Protocol Extension for discovering services external to the XMPP network.
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In-Band Registration
2012Co-Authors: Peter Saint-andreAbstract:This specification defines an XMPP Protocol Extension for in-band registration with XMPP-based instant messaging servers and other services hosted on an XMPP network (such as groupchat rooms and gateways to non-XMPP IM services). The Protocol is extensible via use of data forms, thus enabling services to gather a wide range of information during the registration process. The Protocol also supports in-band password changes and cancellation of an existing registration.
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Unique Room Names for Multi-User Chat
2011Co-Authors: Peter Saint-andreAbstract:This specification defines an XMPP Protocol Extension for requesting a unique room ID from a multi-user chat service.
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Stanza Interception and Filtering Technology (SIFT)
2011Co-Authors: Joe Hildebrand, Jack Moffitt, Peter Saint-andreAbstract:This specification defines an XMPP Protocol Extension that enables a client to exercise control over the XML stanzas it will receive from the server by instructing the server to intercept and filter inbound stanzas.
Joanna Szczurkowska - One of the best experts on this subject based on the ideXlab platform.
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Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe
Nature Protocols, 2016Co-Authors: Joanna Szczurkowska, Andrzej W. Cwetsch, Diego Ghezzi, Gian Michele Ratto, Marco Dal Maschio, Laura CanceddaAbstract:This Protocol is an Extension to: Nat. Protoc. 1 , 1552–1558 (2006); doi:10.1038/nprot.2006.276; published online 9 November 2006 This article describes how to reliably electroporate with DNA plasmids rodent neuronal progenitors of the hippocampus; the motor, prefrontal and visual cortices; and the cerebellum in utero. As a Protocol Extension article, this article describes an adaptation of an existing Protocol and offers additional applications. The earlier Protocol describes how to electroporate mouse embryos using two standard forceps-type electrodes. In the present Protocol, additional electroporation configurations are possible because of the addition of a third electrode alongside the two standard forceps-type electrodes. By adjusting the position and polarity of the three electrodes, the electric field can be directed with great accuracy to different neurogenic areas. Bilateral transfection of brain hemispheres can be achieved after a single electroporation episode. Approximately 75% of electroporated embryos survive to postnatal ages, and depending on the target area, 50–90% express the electroporated vector. The electroporation procedure takes 1 h 35 min. The Protocol is suitable for the preparation of animals for various applications, including histochemistry, behavioral studies, electrophysiology and in vivo imaging. This Protocol Extension describes how to electroporate rodent neuronal progenitors in utero with greater accuracy by using triple-electrode probes.
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Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe
Nature protocols, 2016Co-Authors: Joanna Szczurkowska, Andrzej W. Cwetsch, Marco Dal Maschio, Diego Ghezzi, Gian Michele Ratto, Laura CanceddaAbstract:This Protocol Extension describes how to electroporate rodent neuronal progenitors in utero with greater accuracy by using triple-electrode probes.
Stefania Giacomello - One of the best experts on this subject based on the ideXlab platform.
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Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays
Nature Protocols, 2018Co-Authors: Stefania Giacomello, Joakim LundebergAbstract:This Protocol Extension describes how to prepare plant tissue to enable Spatial Transcriptomics profiling. Spatial Transcriptomics is achieved through the combination of histological staining of the plant tissue with spatially resolved RNA sequencing. Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This Protocol Extension is an adaptation of a Protocol that describes how to use barcoded oligo-dT microarrays to evaluate spatial global gene expression profiles in mammalian tissue to enable it to be applied to plant material. Here, we explain the required adjustments for preparing and treating plant tissue sections on the array surface, specifically in regard to how to permeabilize and remove the tissue. Once the tissue has been removed, the cDNA–mRNA hybrid that is left on the slide is processed in the same way as cDNA obtained during experiments on mammalian tissue; thus the later stages of the Protocol are not included here, and readers should follow the accompanying Protocol for those. We have previously used our Protocol to generate high-quality sequencing libraries for Arabidopsis thaliana inflorescence, Populus tremula developing and dormant leaf buds, and Picea abies female cones. However, we anticipate that the Protocol can be adapted to other tissue types and species. The entire Protocol for preparing samples and processing libraries can be completed in 3–4 d.
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Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays
Nature protocols, 2018Co-Authors: Stefania Giacomello, Joakim LundebergAbstract:Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This Protocol Extension is an adaptation of a protoc ...
