The Experts below are selected from a list of 171 Experts worldwide ranked by ideXlab platform
Hiroyoshi Takano - One of the best experts on this subject based on the ideXlab platform.
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bending of Protonema cells in a plastid glycolate glycerate transporter knockout line of physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
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Bending of Protonema Cells in a Plastid Glycolate/Glycerate Transporter Knockout Line of Physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
Jin Nakahara - One of the best experts on this subject based on the ideXlab platform.
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bending of Protonema cells in a plastid glycolate glycerate transporter knockout line of physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
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Bending of Protonema Cells in a Plastid Glycolate/Glycerate Transporter Knockout Line of Physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
A Szweykowska - One of the best experts on this subject based on the ideXlab platform.
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fine structure ot the Protonema in the moss ceratodon purpureus and its response to a cytokinin
Acta Societatis Botanicorum Poloniae, 2015Co-Authors: Krystyna Idzikowska, A SzweykowskaAbstract:Fine structure of the Protonema is described, with a special attention to its differentiation depending on the position of cells in the protanemal filament, as well as in response to a cytokinin treatment. Complexes of micro-filaments with osmiophilic globules represented structures of particular interest. They appeared temporarily, almost exclusively in apical cells. The cytokinin treatment resulted in the apical cells in an increased number of cytokinetic figures and in structural changes indicating increased metabolic activity. In the intercalary cells, changes in response to the cytokinin were much smaller and mostly concerned an augmented development of the thylakoid system in chloroplasts. After a prolonged (5 days) treatment, degeneration symptoms developed in all cells, particularly in nuclei and chloroplasts, whereas the structure of mitochondria was relatively stable. The results are compared with the observations concerning the cytokinin-induced gametophore buds and with the data of biochemical and physiological investigations of the Protonema.
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fine structure of kinetin treated Protonema and kinetin induced gametophore buds in funaria hygrometrica
Acta Societatis Botanicorum Poloniae, 2015Co-Authors: Fortunat Mlodzianowski, A SzweykowskaAbstract:Besides occasional hypertrophy of grana and disintegration of stroma thylakoids occurring in some chloroplasts, no significant changes were found in ultrastructure of typical Protonema cells treated for six days with kinetin. On the other hand, the fine structure of cells in kinetin--induced gametophore buds differed much from that of the Protonema cells and showed characteristics of cells of with high metabolic activity and high division rates. The results indicate that cytokinins enhance development and differentiation in the Protonema by activating only some of its cells, whereas the others remain unchanged or show symptoms of destruction and ageing. This is supported by the fact that in the presence of chloramphenicol, which prevents bud induction, kinetin acts synergistically with the inhibitor in producing degeneration and destruction of chloroplasts.
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changes in cell wall structure during the Protonema development on organic medium in conditions of light and darkness in funaria hygrometrica
Acta Societatis Botanicorum Poloniae, 2015Co-Authors: Fortunat Mlodzianowski, Adam Woźny, A SzweykowskaAbstract:The cell walls of a Protonema of Funaria hygrometrica cultivated in glucose containing medium were considerably thicker in dark than in light. After a prolonged time of dank culture, a considerable reduction of the wall thicknes was observed, simultaneously with the occurrence of vesicles and plasmalemma invaginations containing fibrillar material. It is suggested that in conditions unfavourable for growth, the sugar taken up from the medium can be accumulated in cell walls, from which it can be mobilized again in conditions of starvation. The authors also think that similar mechanism and cell structures can be involved in both building and decomposition of the cell wall.
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autoradiographic analysis of the effect of cytokinin on protein and rna syntheses in the ceratodon purpureus Protonema
Acta Societatis Botanicorum Poloniae, 2014Co-Authors: Adam Woźny, Urszula Nowak, A SzweykowskaAbstract:In the Protonema of Ceratodon purpureus (Hedw.) Brid., apical parts of the Protonemal filaments (apical cells, initials of Protonemal side branches and of gametophore buds) proved to be preferential sites of [ 14 C]-leucine incorporation into proteins. In some filaments, a similar preference for [ 3 H]-uridine incorporation into RNA was observed, whereas in others there was a rather uniform distribution of label over all cells. A short (0.5-2 h) treatment with cytokinin (N 6 -2-isopentenyladenine) enhanced [ 14 C]-leucine incorporation, without changing the relative distribution of label. No such enhancement, as well as no change in label distribution could be observed in [ 3 H]-uridine incorporation. No direct relationship seems to exist between the early promotion of protein synthesis by cytokinin in the Protonema and cytokinin induction of gametophore buds.
Susumu Takio - One of the best experts on this subject based on the ideXlab platform.
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bending of Protonema cells in a plastid glycolate glycerate transporter knockout line of physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
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Bending of Protonema Cells in a Plastid Glycolate/Glycerate Transporter Knockout Line of Physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
Fumiyoshi Myouga - One of the best experts on this subject based on the ideXlab platform.
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bending of Protonema cells in a plastid glycolate glycerate transporter knockout line of physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
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Bending of Protonema Cells in a Plastid Glycolate/Glycerate Transporter Knockout Line of Physcomitrella patens
PLOS ONE, 2015Co-Authors: Jin Nakahara, Katsuaki Takechi, Fumiyoshi Myouga, Yasuko Moriyama, Hiroshi Sato, Susumu Takio, Hiroyoshi TakanoAbstract:Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB–GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their Protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, Protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.
