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Lorenzo Burgos - One of the best experts on this subject based on the ideXlab platform.
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Apricot ( Prunus armeniaca L.)
Methods of Molecular Biology, 2014Co-Authors: Cesar Petri, Nuria Alburquerque, Lorenzo BurgosAbstract:A protocol for Agrobacterium-mediated stable transformation of whole leaf explants of the apricot (Prunus armeniaca) cultivars 'Helena' and 'Canino' is described. Regenerated buds were selected using a two-step selection strategy with paromomycin sulfate and transferred to bud multiplication medium 1 week after they were detected for optimal survival. After buds were transferred to bud multiplication medium, antibiotic was changed to kanamycin and concentration increased gradually at each transfer to fresh medium in order to eliminate possible escapes and chimeras. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines, was 5.6%. Green and healthy buds, surviving high kanamycin concentration, were transferred to shoot multiplication medium where they elongated in shoots and proliferated. Elongated transgenic shoots were rooted in a medium containing 70 μM kanamycin. Rooted plants were acclimatized following standard procedures. This constitutes the only transformation protocol described for apricot clonal tissues and one of the few of Prunus.
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Efficient in vitro shoot regeneration from mature apricot (Prunus armeniaca L.) cotyledons
Scientia Horticulturae, 2013Co-Authors: Hong Wang, Cesar Petri, Lorenzo Burgos, Nuria AlburquerqueAbstract:Abstract Apricot (Prunus armeniaca L.) is widely considered as a recalcitrant species for shoot regeneration and genetic transformation. Previous reports indicated the possibility to obtain good regeneration from apricot hypocotyl slices; however, we report here an efficient and novel procedure to obtain direct shoot regeneration from the proximal zone of mature apricot cotyledons. Additionally, the system worked for all genotypes tested, which makes this protocol a very interesting and useful tool. Regeneration percentages were influenced by plant growth regulators and the initial dark incubation period, the former being critical to obtain high regeneration from stored apricot cotyledons. The highest regeneration percentages were achieved with TDZ (4 or 8 μM) and 0.25 μM IBA in combination with two weeks dark incubation period reaching up to 67.2%, 56.8%, 66.7%, 46.3% and 66.7% regeneration percentages for ‘Canino’, ‘Dorada’, ‘Moniqui’, ‘Real Fino’ and ‘ansu Maxim’ (Prunus armeniaca L. var. ansu Maxim), respectively. Regeneration pattern appeared as multiple shoots per cotyledon. Histological studies showed that epidermal and sub-epidermal cells of the cotyledon were the cells from where direct organogenesis occurred. To the best of our knowledge, this is the first report of direct in vitro regeneration from mature apricot cotyledons.
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agrobacterium mediated transformation of apricot Prunus armeniaca l leaf explants
Plant Cell Reports, 2008Co-Authors: Cesar Petri, Nuria Alburquerque, Hong Wang, Mohamed Faize, Lorenzo BurgosAbstract:A protocol for Agrobacterium-mediated stable transformation for scored, whole leaf explants of the apricot (Prunus armeniaca) cultivar Helena was developed. Regenerated shoots were selected using a two-step increased concentrations of paromomycin sulphate. Different factors affecting survival of transformed buds, including possible toxicity of green fluorescent protein (GFP) and time of exposure to high cytokine concentration in the regeneration medium, were examined. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines was 5.6%, when optimal conditions for bud survival were provided. Southern blot analysis on four randomly chosen PCR-positive shoots confirmed the presence of the nptII transgene. This is the first time that stable transformation of an apricot cultivar is reported and constitutes also one of the few reports on the transformation of Prunus cultivars.
Birgit Kalthoff - One of the best experts on this subject based on the ideXlab platform.
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regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of plum pox virus
Plant Cell Reports, 1992Co-Authors: Margit Laimer Da Câmara Machado, Artur Da Câmara Machado, V Hanzer, H Weiss, F Regner, Herta Steinkellner, Diethard Mattanovich, Regina Plail, Elisabeth Knapp, Birgit KalthoffAbstract:A system was developed which allows the transfer of foreign genes into apricot cultivars. We report the transformation and regeneration of Prunus armeniaca plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene s-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV). The marker gene GUS was used for optical evaluation of the efficiency of the transformation system. The coat protein gene of PPV was used to introduce coat protein mediated resistance against one of the most important pathogens of stone fruit trees in Europe and the whole Mediterranean area. This is the first report of the successful integration of a viral coat protein gene into a fruit tree species, opening a new perspective on the control of the disease.
D Tricon - One of the best experts on this subject based on the ideXlab platform.
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Genome-wide association links candidate genes to resistance to Plum Pox Virus in apricot (Prunus armeniaca)
New Phytol, 2016Co-Authors: Sicard Mariette, Antoine Barre, S. Decroocq, A. Chague, Fabienne Wong Jun Tai, G Roch, Y Laizet, Alexis Groppi, P. Lambert, D TriconAbstract:In fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. However, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. To address this problem for Plum Pox Virus (PPV) candidate resistance gene identification in Prunus species, we implemented a genome-wide association (GWA) approach in apricot. This study exploited the broad genetic diversity of the apricot (Prunus armeniaca) germplasm containing resistance to PPV, next-generation sequence-based genotyping, and the high-quality peach (Prunus persica) genome reference sequence for single nucleotide polymorphism (SNP) identification. The results of this GWA study validated previously reported PPV resistance quantitative trait loci (QTL) intervals, highlighted other potential resistance loci, and resolved each to a limited set of candidate genes for further study. This work substantiates the association genetics approach for resolution of QTL to candidate genes in apricot and suggests that this approach could simplify identification of other candidate genes for other marked trait intervals in this germplasm.
J M Audergon - One of the best experts on this subject based on the ideXlab platform.
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transcriptomic study of apricot fruit Prunus armeniaca ripening among 13 006 expressed sequence tags
Physiologia Plantarum, 2005Co-Authors: Jeanpaul Bouchet, Patrick Lambert, J M Audergon, Jerome Grimplet, Charles Romieu, Isabelle Marty, G Albagnac, Nancy TerrierAbstract:To improve the knowledge of fruit ripening and to provide genomic resources for molecular breeding of apricot (Prunus armeniaca L), 13 006 expressed sequence tags (ESTs) were generated from three λzap cDNA libraries of the pericarp tissues at different stages of development (Physiol Plant 105: 294-303), yielding 5219 (40%) Unigenes. At this stage, the very low interlibrary redundancy indicated that EST sampling of the transcriptome of apricot pericarp is still far from being saturated. Seventy-six percent of Unigenes displayed homologies with public sequences and were clustered into functional categories. The largest expressed categories were related to primary metabolism, stress response, and protein synthesis. Electronic Northern analysis revealed that stress-related proteins and cell wall modification-related enzymes strongly increased during ripening. Among 448 isoproteins (amino acid-level isogenes) detected in the Unigene set, 186 (42%) displayed significant homologies in their coding regions (nucleic acid-level isogenes).
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genomic and cdna microsatellites from apricot Prunus armeniaca l
Molecular Ecology Notes, 2004Co-Authors: L S Hagen, J Chaib, Bruno Fady, Veronique Decroocq, Jeanpaul Bouchet, Patrick Lambert, J M AudergonAbstract:We developed primers for the amplification of 24 polymorphic nuclear microsatellites in apricot (Prunus armeniaca L.). Thirteen loci originated from three genomic libraries enriched for TC, TG and AAG motifs. Eight loci were developed from three fruit EST (Expressed-Sequence-Tag) libraries and three from a leaf cDNA microsatellite-enriched library. There were up to nine alleles per polymorphic locus in 12 different cultivars. No difference in allele numbers were shown between cDNA and genomic-source loci. Mean expected heterozygosity was 0.65 (range: 0.15–0.87). Mendelian segregation was confirmed for all loci. These markers should be helpful for diversity studies, genome mapping and cultivar identification in apricot and related species.
J Rodrigo - One of the best experts on this subject based on the ideXlab platform.
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Ovary starch reserves and flower development in apricot (Prunus armeniaca)
Physiologia Plantarum, 2020Co-Authors: J Rodrigo, J. Ignacio Hormaza, Maria HerreroAbstract:In histerant species where flowering takes place prior to leaf emergence, a flower lifespan occurs in the absence of new photoassimilates and at the expense of pre-stored reserves either in the plant as a whole or in the flower itself. In the present study, the role that the photoassimilates stored in the flowers might play in flower development from anthesis to fertilization in Prunus armeniaca L. (apricot), a histerant species, was explored. Starch content in individual flowers was measured with the help of an image analysis system. Starch content decreased from its highest value at anthesis and disappeared from the ovary 9 days later. This decrease was inversely related to an increase in ovary size and in cell number in the pericarp, suggesting an intraflower, self-supported development. This process is conserved in both pollinated and nonpollinated flowers and therefore seems to be inherent to the flower at anthesis. The onset of fruiting is preceded by the establishment of large differences among ovaries; while some experience continuous growth, others stop growing and eventually drop. Interestingly, large differences in starch content are found among flowers at anthesis. These results are discussed in terms of the possible implications of pre-stored starch in the flower supporting initial flower development.
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stamen development and winter dormancy in apricot Prunus armeniaca
Annals of Botany, 2011Co-Authors: C Julian, J Rodrigo, M HerreroAbstract:Background and Aims In temperate woody perennials, flower bud development is halted during the winter, when the buds enter dormancy. This dormant period is a prerequisite for adequate flowering, is genetically regulated, and plays a clear role in possibly adapting species and cultivars to climatic areas. However, information on the biological events underpinning dormancy is lacking. Stamen development, with clear differentiated stages, appears as a good framework to put dormancy in a developmental context. Here, stamen developmental changes are characterized in apricot (Prunus armeniaca) and are related to dormancy.
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pistil traits and flower fate in apricot Prunus armeniaca
Annals of Applied Biology, 2009Co-Authors: J Rodrigo, M Herrero, J I HormazaAbstract:Although pollination is essential for both seed and fruit set in most angiosperms, even after an adequate pollination, only a fraction of the flowers develop into fruits. The role played by floral traits on reproductive success is well known, but the possible influence of pistil traits has been overlooked, probably because of the difficulty of non-destructive pistil examination. The aim of this work was to examine the influence of several pistil traits on reproductive success in apricot (Prunus armeniaca). For this purpose, in a population of individually labelled flowers, the styles were cut off once the pollen tubes had reached the ovary but prior to the achievement of fertilisation. This approach allowed relating several morphological and physiological pistil parameters in the dissected styles and stigmas to the subsequent set or abscission of the corresponding ovary that remained in the plant. Under the same pollination conditions, the flowers that finally set a fruit show a larger stigmatic area and a higher number of pollen grains, pollen tubes growing along the style and xylem vessels surrounding the transmitting tissue than flowers that abscise before the establishment of fruit set. Furthermore, starch is present in the transmitting tissue of the style in all the flowers that develop into fruits but only in half of the flowers that abscise. The examined pistil traits established prior to fertilisation are related to flower fate, suggesting that the capacity of a flower to become a fruit could be preconditioned at anthesis.
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influence of intraovular reserves on ovule fate in apricot Prunus armeniaca l
Sexual Plant Reproduction, 1998Co-Authors: J Rodrigo, M HerreroAbstract:In many plant species with multiovulate ovaries, a considerable reduction in the number of ovules takes place. However, the underlying physiological causes are not clear. In Prunus spp., although flowers present two ovules, usually only one seed is produced. We have followed the development and degeneration of the two ovules in apricot (Prunus armeniaca L.) and examined the extent to which carbohydrates within the ovule might be involved in determining the fate of the ovule. While the primary ovule grows in the days following anthesis, growth of the secondary ovule is arrested. Starch distribution along the different ovular tissues exhibits several changes that are different in the two ovules. Primary ovule growth is inversely related to starch content and this growth takes place independently of pollination since it occurs in the same way in pollinated and unpollinated flowers. In the secondary ovule, starch disappears simultaneously from all ovular structures and callose is layered at the chalazal end of the nucellus. The size of the secondary ovule does not change significantly from anthesis to degeneration, and callose starts to accumulate 5 days after anthesis. Likewise, this process occurs independently of pollination. These results are discussed in terms of the implications of the starch content of ovules in fertilization success and ovule fate.
