The Experts below are selected from a list of 2205 Experts worldwide ranked by ideXlab platform
Zhihong Gao - One of the best experts on this subject based on the ideXlab platform.
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Taxonomy and Germplasm of Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:In this chapter, the standard and basis for Prunus mume taxonomy are being discussed. According to its morphological characteristics and distribution, P. mume can be classified into two ecological categories: high-elevation and low-elevation types. Within P. mume species, there are seven varieties and one variant.
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Production and Academic Importance of Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong GaoAbstract:Prunus mume has a great ornamental and nutritional value. The species is considered as an important fruit crop due to its special values. It is distributed throughout Eastern Asia, while the largest production region is southern China. The fruits are used in Chinese traditional medicine.
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Prunus mume Transcriptomics
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:Large-scale transcriptome analysis using next-generation sequencing (NGS) technologies has proven extremely useful to obtain candidates for such unidentified genes. The NGS technologies are capable of generating high-throughput reads at a relatively low cost and have been used in various types of research, including genome sequencing, marker discovery and, in particular, transcriptome analysis. Genome-wide expression analyses are essential tools for elucidating molecular functions. The number of reads generated by the Roche/454 GS FLX is considerably lower than that generated by short-read platforms such as the Illumina Genome Analyzer. Transcriptome analysis using NGS technologies can capture nearly all the expressed sequences, including rare transcripts in a particular tissue at a specific developmental stage, due to the great depth of sequencing. Therefore, this approach is highly useful, especially for transcriptome analysis in non-model organisms such as Prunus mume.
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Origin and Evolution of Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Wenjie LuoAbstract:Prunus mume (P. mume Sieb. et Zucc., 2n = 2x = 16) is a popular ornamental plant widely cultivated in East Asia, where it was domesticated over 3000 years ago. As an important member of the genus Prunus, it plays a pivotal role in phylogenetic studies of the Rosaceae family. To understand the origin of P. mume, it is important to know how the proto-species of P. mume did form and where it has its origin. Specimens of wild P. mume have been discovered in Yunnan Sichuan, Hubei, Guizhou and Tibet, China.
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Small RNAs in Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:Japanese apricot (Prunus mume Sieb. et Zucc) is an important economic fruit crop in China and Japan, with more than 200 cultivars in China. It is one of the most valuable materials used in the food and winemaking industries and is believed to contain many physio-chemicals beneficial for human health. MicroRNAs (miRNAs) are a class of endogenous, small, noncoding RNAs that regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in higher plants. High-throughput sequencing is used to identify and quantitatively profile small RNAs in Japanese apricot. Previous studies have shown comparative identification of miRNAs between perfect and imperfect Japanese apricot flowers and some small RNAs related to the flower opening process.
Tan Zhi-gang - One of the best experts on this subject based on the ideXlab platform.
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Propagation Experiment of Prunus mume by Hardwood Cuttage
Guangdong Agricultural Sciences, 2011Co-Authors: Tan Zhi-gangAbstract:Using the one-year-old hardwood branches of Prunus mume as cuttings,the factors affecting the propagation of Prunus mume by cuttage were studied.The results revealed that the genotype,concentration of rooting powder,segment part of branch significantly affected the survival rate of Prunus mume cuttings.The cuttings of Yingsu Prunus mume showed the highest survival rate,being 90%.Immersing the hardwood branches in 200 mg/L rooting powder solution for 2 h got the best propagation effect,and the survival rate of the cuttings reached 80%.Taking the middle part of hardwood branches as cutting was best for the survival of the cuttings.
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Research and Utilization of Prunus mume Germplasm Resources in China
Tianjin Agricultural Sciences, 2011Co-Authors: Tan Zhi-gangAbstract:The paper introduced the geographical distribution of Prunus mume,germplasm resources and their classification,the biological characteristics of pollination and fertilization,and analyzed utilization and problem of germplasm resources.The emphasis of research on Prunus mume germplasm resources were suggested.
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Effects of Different Treatments on Seed Germination of Prunus mume
Northern Horticulture, 2011Co-Authors: Tan Zhi-gangAbstract:Used better sellected plant('Qianli No.1') seed from Prunus mume as materials,effect on germination rates of seeds by the method of GA3,stratification and removing endocarp were studied.The results showed that the optimal soaking concentration of GA3 was 50 mg/L,its germination rate was 32%.When soaking the seeds with 150 mg/L GA3,the suitable soaking time was 30 h.The seeds under low temperature at 4℃ could got a certain percentage of germination 33% after about 60 days.It could increase the germination rate significantly by removing endocarp,the percentage of germination reached 47%.
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A Preliminary Study of Prunus mume in Vitro Propagation
Northern Horticulture, 2010Co-Authors: Tan Zhi-gangAbstract:Using better single plant(Qian Li NO.1) from Prunus mume as materials,explants sterilization,and proliferation culture of axillary buds were studied.The results showed that the optimal sterilization time of explant was about 8 minutes with 0.1%HgCl_2,and its survival rate was 75%.The basal medium of of axillary buds was WPM.The best medium for and proliferation of axillary buds was WPM+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA_3,and its effective multiplication coefficient was 1.51.
Ting Shi - One of the best experts on this subject based on the ideXlab platform.
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Taxonomy and Germplasm of Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:In this chapter, the standard and basis for Prunus mume taxonomy are being discussed. According to its morphological characteristics and distribution, P. mume can be classified into two ecological categories: high-elevation and low-elevation types. Within P. mume species, there are seven varieties and one variant.
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Prunus mume Transcriptomics
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:Large-scale transcriptome analysis using next-generation sequencing (NGS) technologies has proven extremely useful to obtain candidates for such unidentified genes. The NGS technologies are capable of generating high-throughput reads at a relatively low cost and have been used in various types of research, including genome sequencing, marker discovery and, in particular, transcriptome analysis. Genome-wide expression analyses are essential tools for elucidating molecular functions. The number of reads generated by the Roche/454 GS FLX is considerably lower than that generated by short-read platforms such as the Illumina Genome Analyzer. Transcriptome analysis using NGS technologies can capture nearly all the expressed sequences, including rare transcripts in a particular tissue at a specific developmental stage, due to the great depth of sequencing. Therefore, this approach is highly useful, especially for transcriptome analysis in non-model organisms such as Prunus mume.
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Small RNAs in Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:Japanese apricot (Prunus mume Sieb. et Zucc) is an important economic fruit crop in China and Japan, with more than 200 cultivars in China. It is one of the most valuable materials used in the food and winemaking industries and is believed to contain many physio-chemicals beneficial for human health. MicroRNAs (miRNAs) are a class of endogenous, small, noncoding RNAs that regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in higher plants. High-throughput sequencing is used to identify and quantitatively profile small RNAs in Japanese apricot. Previous studies have shown comparative identification of miRNAs between perfect and imperfect Japanese apricot flowers and some small RNAs related to the flower opening process.
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Botanical Description of Prunus mume
Compendium of Plant Genomes, 2019Co-Authors: Zhihong Gao, Ting ShiAbstract:Prunus mume is a small deciduous arbour tree species with a weak trunk and a shallow and broad root system. The flower buds of P. mume are covered with scales, and usually, one bud has only one flower. Branching is weak and gradually weakens with increasing tree age; the majority of the long twigs are located in the middle and upper parts of the branches. Adaxial leaf surfaces in P. mume bear hairs on young leaves, which gradually fall as the leaves mature. The fruiting branches of P. mume trees can be divided into six categories: water sprouts, long branches, middle branches, short branches, tiny branches and needle branches. According to the characteristics of morphological differentiation and biochemical changes, and based on the differentiation period of the sepal, in the stage of morphological differentiation, we distinguish flower primordium differentiation and the differentiation of flower organs. The pistil of P. mume consists of a single carpel, and the ovary is superior. There are generally three peak fruit-drop periods in the early stage, and some varieties show fruit-drop before harvest. The phenological period of P. mume varies with region and variety. Its main feature is early flowering, first flowering and then leafing; the fruit growth and development periods are short, and leaf drop is early.
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Genome-wide discovery and characterization of flower development related long non-coding RNAs in Prunus mume
BMC, 2019Co-Authors: Ting Shi, Shahid Iqbal, Yong Zhang, Lin Liu, Zhihong GaoAbstract:Abstract Background Long non-coding RNAs (lncRNAs) are transcripts more than 200 bp in length do not encode proteins. Up to the present, it has been reported that lncRNAs play an essential role in developmental processes through their regulatory functions. However, their characteristics, expression inheritance patterns, and functions in Prunus mume are quite unidentified. Results In this present study, we exposed the specific characters of pistil development process between single pistil cv ‘Qingjia No.2’ (QJN2) and multiple pistils cv ‘Da Yu’ (DY). We found that early October is the key stage for pistil differentiation. The similarity epidermis was observed between two types of pistil. We also further investigated a complete pistil development lncRNA profiles through RNA-seq in Prunus mume. 2572 unique lncRNAs and 24,648 genes mapped to Prunus mume genome, furthermore, 591 novel lncRNAs were predicted. Both unique lncRNAs and novel lncRNAs are shorter in length than the mRNAs, and the overall expression level of lncRNAs was lower than mRNAs in Prunus mume. 186 known lncRNAs, 1638 genes and 89 novel lncRNAs were identified as significant differential expressed in QJN2 compared with DY. We predicted 421 target genes of differentially expressed known lncRNAs (DEKLs) and 254 target genes of differentially expressed novel lncRNAs (DENLs). 153 miRNAs were predicted interacted with 100 DEKLs while 112 miRNAs were predicted interacted with 55 DENLs. Further analysis of the DEKLs showed that the lncRNA of XR_514690.2 down-regulated its target ppe-miR172d, and up-regulated AP2, respectively. Meanwhile, the other lncRNA of TCONS_00032517 induced cytokinin negative regulator gene A-ARR expression via repressing its target miRNA ppe-miR160a/b in DY. At the same time we found that the AP2 expression was significantly up-regulated by zeatin (ZT) treatment in flower buds. Our experiments suggest that the two lncRNAs of XR_514690.2 and TCONS_00032517 might contribute the formation of multiple pistils in Prunus mume. Conclusion This study shows the first characterization of lncRNAs involved in pistil development and provides new indications to elucidate how lncRNAs and their targets play role in pistil differentiation and flower development in Prunus mume
Zhang Shao-ling - One of the best experts on this subject based on the ideXlab platform.
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Effects of Temperature on Recognition Specificity of Stylar S-RNase in vitro in Prunus mume
2008Co-Authors: Zhang Shao-lingAbstract:【Objective】Effects of temperature on degradation of pollen tube RNA by the stylar S-RNase from Prunus mume were investigated, which provided an experimental basis for elucidating the mechanisms of self-incompatibility in Prunus mume. 【Method】Adding purified stylar S-RNase from Prunus mume cultivar ‘Gessekai’ to liquid medium, for cultivating self- (‘Gessekai’) and cross-pollen (‘Oushuku’) at temperatures 10℃, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time. The degradation of pollen tube RNA in vitro by stylar S-RNase at different temperatures was performed to detect the extent of degradation using the agarose gel electrophoresis. 【Result】The germination and tube growth of self-pollen and the RNA of self-pollen tube were inhibited and degraded significantly greater than cross-pollen respectively at 10℃ and 25℃ , but no significant difference when the temperature rose to 35℃ . The specific inhibition of a large degree for self-pollen germination and tube growth and the specific degradation for self-pollen tube RNA gradually weakened as the temperature increased and disappeared when the temperature came to 35℃ . 【Conclusion】Specific recognition and degradation of stylar S-RNase for self-pollen tube RNA, resulted in the stop of self-tube growth in Prunus mume. The recognition specificity of stylar S-RNase for the RNA weakened with the rise of temperature, consequently regulating the self-incompatibility reaction in Prunus mume.
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Characteristics of Pollen Germination and Pollen Tube Growth of Prunus mume in Vitro
2006Co-Authors: Zhang Shao-ling, Jiang Xue-tingAbstract:The study examined pollen germinations and pollen tube growth of Prunus mume Sieb.Let Zucc.cultured with different medium components at different pollen densities and different temperatures and different times.It was shown that in Xiyeqing,the optimal medium of pollen germination and pollen tube growth was 30 mmol/L MES buffer(pH 6.5) containing 20% sucrose,0.01% H_3BO_3,20% PEG-4000,(0.03)% Ca(NO_3)_2 ·4H_2O and 0.02% MgSO_4·7H_2O;on the medium,the germination rate reached(45.03)% and the pollen tube length reached 597.2 μm;and the optimal pollen density of pollen germination and pollen tube growth was 20～80 pollens/mL.In Prunus mume too high and too low a culture temperature were not favorable for pollen germination and pollen tube growth and optimal pollen germination and optimal pollen tube growth appeared at 25℃.The average pollen germinations of the tree varieties,Xiyeqing,Gessekai and Oushuku was 48.6% and their average pollen tube length was 762.3 μm.Cultured at different temperatures Prunus mume pollens germinated differently,and cultured at 25℃ the pollen tubes grew most quickly,mainly within 0 ～12 hours,and the average pollen tube growth rate of the tree varieties was 58.5 μm/h.
Jiang Xue-ting - One of the best experts on this subject based on the ideXlab platform.
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Characteristics of Pollen Germination and Pollen Tube Growth of Prunus mume in Vitro
2006Co-Authors: Zhang Shao-ling, Jiang Xue-tingAbstract:The study examined pollen germinations and pollen tube growth of Prunus mume Sieb.Let Zucc.cultured with different medium components at different pollen densities and different temperatures and different times.It was shown that in Xiyeqing,the optimal medium of pollen germination and pollen tube growth was 30 mmol/L MES buffer(pH 6.5) containing 20% sucrose,0.01% H_3BO_3,20% PEG-4000,(0.03)% Ca(NO_3)_2 ·4H_2O and 0.02% MgSO_4·7H_2O;on the medium,the germination rate reached(45.03)% and the pollen tube length reached 597.2 μm;and the optimal pollen density of pollen germination and pollen tube growth was 20～80 pollens/mL.In Prunus mume too high and too low a culture temperature were not favorable for pollen germination and pollen tube growth and optimal pollen germination and optimal pollen tube growth appeared at 25℃.The average pollen germinations of the tree varieties,Xiyeqing,Gessekai and Oushuku was 48.6% and their average pollen tube length was 762.3 μm.Cultured at different temperatures Prunus mume pollens germinated differently,and cultured at 25℃ the pollen tubes grew most quickly,mainly within 0 ～12 hours,and the average pollen tube growth rate of the tree varieties was 58.5 μm/h.
