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Hong Ji - One of the best experts on this subject based on the ideXlab platform.
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Metabolites of Puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and intestine of rats.
Journal of Chromatography B, 2010Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Abstract Puerarin is a major active ingredient of Pueraria Radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate Puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID–MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1 × 100 mm, 1.8 μm) at 30 °C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min −1 , and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min −1 , dry temperature: 350 °C, nebulizer: 40 psi, capillary: −3500 V, scan range: 250–800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of Puerarin (M1 and M2) were detected. M1 and M2 are presumed to be Puerarin-7- O -glucuronide and Puerarin-4′- O -glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of Puerarin, the levels of M1 and M2 then reached their peaks at 10–15 and 15–30 min, respectively. The metabolic profiles were similar in rat liver and intestine investigated by in situ liver and intestine perfusion, indicating that no metabolic regioselectivity of Puerarin occurs in the two organs.
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Metabolites of Puerarin identified by liquid chromatography tandem mass spectrometry: similar metabolic profiles in liver and intestine of rats.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Mu Yuan, Min-sheng Chen, Hong JiAbstract:Puerarin is a major active ingredient of Pueraria radix. Puerarin may exert its medicinal functions in part via its metabolites. In this study, we identified these metabolites to better understand and elucidate Puerarin's metabolic pathway. Puerarin was intravenously administered to rats and then metabolites in plasma samples were identified by rapid resolution liquid chromatography electrospray ionization-collision induced dissociation tandem mass spectrometry (RRLC-ESI-CID-MS/MS). Chromatography was conducted on a Zorbax SB C18 column (2.1x100 mm, 1.8 microm) at 30 degrees C, with a gradient mobile phase consisting of 0.05% formic acid and acetonitrile, a flow rate of 0.2 mL min(-1), and a total run time of 14 min. MS/MS acquisition parameters were as follows: positive ionization mode, dry gas: nitrogen, 10 L min(-1), dry temperature: 350 degrees C, nebulizer: 40 psi, capillary: -3500 V, scan range: 250-800. The autoMS, manual, or multiple reaction monitoring mode was selected as required. Two glucuronidated metabolites of Puerarin (M1 and M2) were detected. M1 and M2 are presumed to be Puerarin-7-O-glucuronide and Puerarin-4'-O-glucuronide, respectively, and M2 likely is suspected to be the major metabolite because it represented the predominate peak. Kinetic studies of metabolites demonstrated that M1 and M2 were detected in rat plasma at 5 min after intravenous administration of Puerarin, the levels of M1 and M2 then reached their peaks at 10-15 and 15-30 min, respectively. The metabolic profiles were similar in rat liver and intestine investigated by in situ liver and intestine perfusion, indicating that no metabolic regioselectivity of Puerarin occurs in the two organs.
Stephen Barnes - One of the best experts on this subject based on the ideXlab platform.
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identification of isoflavone glycosides in pueraria lobata cultures by tandem mass spectrometry
Phytochemical Analysis, 2007Co-Authors: Jeevan K Prasain, Kenneth Jones, Stephen Barnes, Adam Reppert, Ray D Moore, Mary Ann LilaAbstract:Isoflavones in the methanolic extracts of kudzu (Pueraria lobata) callus, suspension and root cultures were compared in order to develop an experimental system in which Puerarin (daidzein 8-C-glucoside) and other isoflavones could be synthesised in vitro. Quantitative variation of Puerarin and other known isoflavones was estimated in kudzu culture extracts using HPLC-UV. The highest and lowest amounts of Puerarin (14.56 and 0.33 mg/g) were present in in vitro root cultures and leaf tissue-derived callus cultures, respectively. A total of 48 isoflavone metabolites were detected in extracts of kudzu root cultures by HPLC-MS/MS, and the structures of 33 of them were tentatively assigned. Amongst these, 12 isoflavone C-glycosides were identified. Hydroxy-derivatives of Puerarin in several isomeric forms were detected, some of which have not been previously reported in kudzu root. The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites. Copyright © 2006 John Wiley & Sons, Ltd.
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identification of Puerarin and its metabolites in rats by liquid chromatography tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Jeevan K Prasain, Kenneth Jones, Nancy Brissie, Ray Moore, Michael J Wyss, Stephen BarnesAbstract:Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Pueraria lobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of Puerarin and its metabolites daidzein, dihydrodaidzein, and equol was developed for their analysis in biological samples. Two new metabolites of Puerarin, mono- and dihydroxylated derivatives, were detected in the urine and feces of rats after oral administration. The persistence of Puerarin in blood and urine as the principal metabolic form for the period of 4-72 h after oral administration suggested that Puerarin is rapidly absorbed from the intestine without metabolism. Its presence in organs such as the brain suggests that this glucoside may enter tissues by specific transport pathways. Study of these metabolites may provide further understanding of the health beneficial effects of Puerarin in kudzu dietary supplements.
Jeevan K Prasain - One of the best experts on this subject based on the ideXlab platform.
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identification of isoflavone glycosides in pueraria lobata cultures by tandem mass spectrometry
Phytochemical Analysis, 2007Co-Authors: Jeevan K Prasain, Kenneth Jones, Stephen Barnes, Adam Reppert, Ray D Moore, Mary Ann LilaAbstract:Isoflavones in the methanolic extracts of kudzu (Pueraria lobata) callus, suspension and root cultures were compared in order to develop an experimental system in which Puerarin (daidzein 8-C-glucoside) and other isoflavones could be synthesised in vitro. Quantitative variation of Puerarin and other known isoflavones was estimated in kudzu culture extracts using HPLC-UV. The highest and lowest amounts of Puerarin (14.56 and 0.33 mg/g) were present in in vitro root cultures and leaf tissue-derived callus cultures, respectively. A total of 48 isoflavone metabolites were detected in extracts of kudzu root cultures by HPLC-MS/MS, and the structures of 33 of them were tentatively assigned. Amongst these, 12 isoflavone C-glycosides were identified. Hydroxy-derivatives of Puerarin in several isomeric forms were detected, some of which have not been previously reported in kudzu root. The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites. Copyright © 2006 John Wiley & Sons, Ltd.
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identification of Puerarin and its metabolites in rats by liquid chromatography tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Jeevan K Prasain, Kenneth Jones, Nancy Brissie, Ray Moore, Michael J Wyss, Stephen BarnesAbstract:Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Pueraria lobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of Puerarin and its metabolites daidzein, dihydrodaidzein, and equol was developed for their analysis in biological samples. Two new metabolites of Puerarin, mono- and dihydroxylated derivatives, were detected in the urine and feces of rats after oral administration. The persistence of Puerarin in blood and urine as the principal metabolic form for the period of 4-72 h after oral administration suggested that Puerarin is rapidly absorbed from the intestine without metabolism. Its presence in organs such as the brain suggests that this glucoside may enter tissues by specific transport pathways. Study of these metabolites may provide further understanding of the health beneficial effects of Puerarin in kudzu dietary supplements.
Miaoxian Dong - One of the best experts on this subject based on the ideXlab platform.
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protective effect of Puerarin against beta amyloid induced oxidative stress in neuronal cultures from rat hippocampus involvement of the gsk 3β nrf2 signaling pathway
Free Radical Research, 2013Co-Authors: Yu Zou, B Hong, Li Fan, Li Zhou, Y Liu, X Zhang, Miaoxian DongAbstract:AbstractCurrent evidence suggests that amyloid beta (Aβ) peptides may play a major role in the pathogenesis of Alzheimer's disease in part by eliciting oxidative stress. Puerarin, a major isoflavone glycoside from Kudzu root (Pueraria lobata), has been reported to exert estrogen-like and antioxidant activities. The central hypothesis guiding this study is that Puerarin will prevent or at least markedly attenuate Aβ25–35-induced excess production of reactive oxygen species (ROS) by interrupting glycogen synthase kinase-3β (GSK-3β) signaling. In this study, we demonstrate that pretreatment of primary hippocampal neurons with Puerarin significantly reduced Aβ25–35-induced oxidative stress characterized by scavenging of ROS and inhibiting lipid peroxidation. Puerarin induced expression of nuclear Nrf2 protein, but not in the Nrf2 mRNA level, and increased heme oxygenase-1 (HO-1) levels at levels of transcription and translation. Puerarin-induced Serine 9 phosphorylation of GSK-3β was blocked by lithium chlori...
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neuroprotective effects of Puerarin against beta amyloid induced neurotoxicity in pc12 cells via a pi3k dependent signaling pathway
Brain Research Bulletin, 2011Co-Authors: Guihua Xing, Yu Zou, Li Fan, Li Zhou, Miaoxian Dong, Xiaoli Wang, Defu Cai, Jicheng Liu, Yingcai NiuAbstract:Epidemiological data have indicated that estrogen replacement therapy (ERT) can decrease the risk of developing Alzheimer's disease (AD). Phytoestrogens have been proposed as potential alternatives to ERT. The aim of the present study was to assess the neuroprotective effects of Puerarin, a phytoestrogen isolated from Pueraria lobata, against the toxicity of beta-amyloid (Aβ) in relation to the mitochondria-mediated cell death process, and to elucidate the role the activation of Akt and modulation of the pro- and antiapoptotic proteins in Puerarin-induced neuroprotection. The present study shows that Puerarin afforded protection against Aβ-induced toxicity through inhibiting apoptosis in PC12 cells. This result was also confirmed by the activated caspase-3 assay. P-Akt, Bcl-2 and p-Bad expression increased after pretreatment with Puerarin in PC12 cells exposed to Aβ(25-35), whereas Bax expression and cytochrome c release decreased. Interestingly, these effects of Puerarin against Aβ(25-35) insult were abolished by wortmannin, an inhibitor of PI3K phosphorylation. These findings suggest that Puerarin prevent Aβ-induced neurotoxicity through inhibiting neuronal apoptosis, and might be a potential preventive or therapeutic agent for AD.
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Puerarin attenuates amyloid beta induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo
European Journal of Pharmacology, 2010Co-Authors: Gang Wang, Li Zhou, Miaoxian Dong, Jicheng Liu, Rui Wang, Chunrong Lin, Yingcai NiuAbstract:Elevated levels of β-amyloid (Aβ) in the brains being a hallmark of Alzheimer's disease have been believed to play a critical role in the cognitive dysfunction that occurs in Alzheimer's disease. Recent evidence suggests that Aβ induces neuronal apoptosis in the brain and in primary neuronal cultures. In this study, we investigated the effects of Puerarin, a phytoestrogen isolated from Pueraria lobata, on cognitive function and neuronal apoptosis in the intrahippocampal injection of Aβ rats and its mechanism of action. The results show the intrahippocampal injection of Aβ induced a spatial memory deficit, apoptosis, and caspase-9 activation in hippocampal neurons. Puerarin treatment ameliorated Aβ(1-42)-induced cognitive impairment and reversed the increase of apoptosis in the hippocampus. The attenuation is associated with the activation of Akt and phosphorylation of Bad. These results suggest that Puerarin may be an anti-Alzheimer's disease candidate drug to suppress both Alzheimer's disease-related neuronal cell apoptosis and dysfunction of the memory system.
Mary Ann Lila - One of the best experts on this subject based on the ideXlab platform.
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isolation of radiolabeled isoflavones from kudzu pueraria lobata root cultures
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Adam Reppert, Gad G Yousef, Randy B Rogers, Mary Ann LilaAbstract:Isoflavones have potential for preventing and treating several chronic health conditions, such as osteoporosis, cardiovascular disease, and cancer. In this study, radiolabeled isoflavones were recovered from kudzu (Pueraria lobata) root cultures after incubation with uniformly labeled (14)C-sucrose in the culture medium for 21 days. Approximately 19% of administered label was recovered in the isoflavone-rich dried extracts of kudzu root cultures (90.2 microCi/g or 3.3 MBq/g extract). HPLC-PDA analysis revealed the predominant isoflavones isolated from kudzu root cultures to be Puerarin, daidzin, and malonyl-daidzin. The average concentration of the major isoflavone Puerarin in kudzu root cultures was 33.6 mg/g extract, with a specific activity of 63.5 microCi/g (2.3 MBq/g). The isolated isoflavones were sufficiently (14)C-labeled to permit utilization for subsequent in vivo metabolic tracking studies.
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identification of isoflavone glycosides in pueraria lobata cultures by tandem mass spectrometry
Phytochemical Analysis, 2007Co-Authors: Jeevan K Prasain, Kenneth Jones, Stephen Barnes, Adam Reppert, Ray D Moore, Mary Ann LilaAbstract:Isoflavones in the methanolic extracts of kudzu (Pueraria lobata) callus, suspension and root cultures were compared in order to develop an experimental system in which Puerarin (daidzein 8-C-glucoside) and other isoflavones could be synthesised in vitro. Quantitative variation of Puerarin and other known isoflavones was estimated in kudzu culture extracts using HPLC-UV. The highest and lowest amounts of Puerarin (14.56 and 0.33 mg/g) were present in in vitro root cultures and leaf tissue-derived callus cultures, respectively. A total of 48 isoflavone metabolites were detected in extracts of kudzu root cultures by HPLC-MS/MS, and the structures of 33 of them were tentatively assigned. Amongst these, 12 isoflavone C-glycosides were identified. Hydroxy-derivatives of Puerarin in several isomeric forms were detected, some of which have not been previously reported in kudzu root. The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites. Copyright © 2006 John Wiley & Sons, Ltd.
