The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Gloria Decarlo Massaro - One of the best experts on this subject based on the ideXlab platform.
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Estrogen receptor-α regulates Pulmonary alveolar loss and regeneration in female mice: morphometric and gene expression studies
American Journal of Physiology-lung Cellular and Molecular Physiology, 2007Co-Authors: Donald Massaro, Linda Biadasz Clerch, Gloria Decarlo MassaroAbstract:Pulmonary Alveoli, especially in females, are estrogen-responsive structures: ovariectomy in wild-type (WT) adult mice results in alveolar loss, and estradiol replacement induces alveolar regenerat...
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Toward Therapeutic Pulmonary Alveolar Regeneration in Humans
Proceedings of the American Thoracic Society, 2006Co-Authors: Donald Massaro, Gloria Decarlo MassaroAbstract:In humans, age results in loss of Pulmonary Alveoli; menopause accelerates loss of diffusing capacity, an index of alveolar surface area; and disease (e.g., chronic obstructive Pulmonary disease) results in loss of Alveoli. Thus, an important goal for investigators is to generate knowledge that allows induction of Pulmonary alveolar regeneration in humans. Our enthusiasm for this goal and our assessment of its feasibility are based on work in several laboratories over the last decade that has disproved the notion that Pulmonary Alveoli are incapable of regeneration, and on the growing evidence that signals that regulate programs of alveolar turnover (loss and regeneration) are conserved from rodents to humans. We review animal models of alveolar loss and regeneration and their conservation during evolution, and hence their relevance to humans.
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noninvasive delivery of small inhibitory rna and other reagents to Pulmonary Alveoli in mice
American Journal of Physiology-lung Cellular and Molecular Physiology, 2004Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Linda Biadasz ClerchAbstract:A technically easy, noninvasive means of delivering molecules to Alveoli, which act selectively or specifically in the lung, would be experimentally and therapeutically useful. As proof of principle, we took advantage of the spreading ability of Pulmonary surface active material (InfaSurf), mixed it with elastase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) small inhibitory RNA (siRNA), or all-trans retinoic acid (ATRA), and instilled microliter amounts of the mixture into the nose of lightly anesthetized mice. One instillation of elastase caused diffuse alveolar destruction (emphysema) demonstrating widespread alveolar delivery. A single nasal instillation of GAPDH siRNA, compared with scrambled GAPDH siRNA, lowered GAPDH protein in lung, heart, and kidney by ∼50–70% 1 and 7 days later. To test the possibility of lung-specific delivery of a potentially therapeutic drug, we administered ATRA and monitored its effect on expression of cellular retinol binding protein (CRBP)-1 mRNA, whose translation pr...
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Calorie-related rapid onset of alveolar loss, regeneration, and changes in mouse lung gene expression
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Alexander S. Baras, Eric P. Hoffman, Linda Biadasz ClerchAbstract:Calorie restriction, followed by ad libitum refeeding, results, respectively, in loss and regeneration of Pulmonary Alveoli. We now show 35% of Alveoli are lost within 72 h of onset of calorie rest...
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Lung retinol storing cells synthesize and secrete retinoic acid, an inducer of alveolus formation
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Ghenima Dirami, Gloria Decarlo Massaro, Linda Biadasz Clerch, Una S. Ryan, Peter R. Reczek, Donald MassaroAbstract:Retinoids play a key role in the formation of Pulmonary Alveoli. Lipid interstitial cells (LICs) of the alveolar wall store retinol and are concentrated at sites of alveolus formation, suggesting t...
Donald Massaro - One of the best experts on this subject based on the ideXlab platform.
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Estrogen receptor-α regulates Pulmonary alveolar loss and regeneration in female mice: morphometric and gene expression studies
American Journal of Physiology-lung Cellular and Molecular Physiology, 2007Co-Authors: Donald Massaro, Linda Biadasz Clerch, Gloria Decarlo MassaroAbstract:Pulmonary Alveoli, especially in females, are estrogen-responsive structures: ovariectomy in wild-type (WT) adult mice results in alveolar loss, and estradiol replacement induces alveolar regenerat...
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Toward Therapeutic Pulmonary Alveolar Regeneration in Humans
Proceedings of the American Thoracic Society, 2006Co-Authors: Donald Massaro, Gloria Decarlo MassaroAbstract:In humans, age results in loss of Pulmonary Alveoli; menopause accelerates loss of diffusing capacity, an index of alveolar surface area; and disease (e.g., chronic obstructive Pulmonary disease) results in loss of Alveoli. Thus, an important goal for investigators is to generate knowledge that allows induction of Pulmonary alveolar regeneration in humans. Our enthusiasm for this goal and our assessment of its feasibility are based on work in several laboratories over the last decade that has disproved the notion that Pulmonary Alveoli are incapable of regeneration, and on the growing evidence that signals that regulate programs of alveolar turnover (loss and regeneration) are conserved from rodents to humans. We review animal models of alveolar loss and regeneration and their conservation during evolution, and hence their relevance to humans.
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noninvasive delivery of small inhibitory rna and other reagents to Pulmonary Alveoli in mice
American Journal of Physiology-lung Cellular and Molecular Physiology, 2004Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Linda Biadasz ClerchAbstract:A technically easy, noninvasive means of delivering molecules to Alveoli, which act selectively or specifically in the lung, would be experimentally and therapeutically useful. As proof of principle, we took advantage of the spreading ability of Pulmonary surface active material (InfaSurf), mixed it with elastase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) small inhibitory RNA (siRNA), or all-trans retinoic acid (ATRA), and instilled microliter amounts of the mixture into the nose of lightly anesthetized mice. One instillation of elastase caused diffuse alveolar destruction (emphysema) demonstrating widespread alveolar delivery. A single nasal instillation of GAPDH siRNA, compared with scrambled GAPDH siRNA, lowered GAPDH protein in lung, heart, and kidney by ∼50–70% 1 and 7 days later. To test the possibility of lung-specific delivery of a potentially therapeutic drug, we administered ATRA and monitored its effect on expression of cellular retinol binding protein (CRBP)-1 mRNA, whose translation pr...
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Calorie-related rapid onset of alveolar loss, regeneration, and changes in mouse lung gene expression
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Alexander S. Baras, Eric P. Hoffman, Linda Biadasz ClerchAbstract:Calorie restriction, followed by ad libitum refeeding, results, respectively, in loss and regeneration of Pulmonary Alveoli. We now show 35% of Alveoli are lost within 72 h of onset of calorie rest...
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Lung retinol storing cells synthesize and secrete retinoic acid, an inducer of alveolus formation
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Ghenima Dirami, Gloria Decarlo Massaro, Linda Biadasz Clerch, Una S. Ryan, Peter R. Reczek, Donald MassaroAbstract:Retinoids play a key role in the formation of Pulmonary Alveoli. Lipid interstitial cells (LICs) of the alveolar wall store retinol and are concentrated at sites of alveolus formation, suggesting t...
Linda Biadasz Clerch - One of the best experts on this subject based on the ideXlab platform.
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Estrogen receptor-α regulates Pulmonary alveolar loss and regeneration in female mice: morphometric and gene expression studies
American Journal of Physiology-lung Cellular and Molecular Physiology, 2007Co-Authors: Donald Massaro, Linda Biadasz Clerch, Gloria Decarlo MassaroAbstract:Pulmonary Alveoli, especially in females, are estrogen-responsive structures: ovariectomy in wild-type (WT) adult mice results in alveolar loss, and estradiol replacement induces alveolar regenerat...
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noninvasive delivery of small inhibitory rna and other reagents to Pulmonary Alveoli in mice
American Journal of Physiology-lung Cellular and Molecular Physiology, 2004Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Linda Biadasz ClerchAbstract:A technically easy, noninvasive means of delivering molecules to Alveoli, which act selectively or specifically in the lung, would be experimentally and therapeutically useful. As proof of principle, we took advantage of the spreading ability of Pulmonary surface active material (InfaSurf), mixed it with elastase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) small inhibitory RNA (siRNA), or all-trans retinoic acid (ATRA), and instilled microliter amounts of the mixture into the nose of lightly anesthetized mice. One instillation of elastase caused diffuse alveolar destruction (emphysema) demonstrating widespread alveolar delivery. A single nasal instillation of GAPDH siRNA, compared with scrambled GAPDH siRNA, lowered GAPDH protein in lung, heart, and kidney by ∼50–70% 1 and 7 days later. To test the possibility of lung-specific delivery of a potentially therapeutic drug, we administered ATRA and monitored its effect on expression of cellular retinol binding protein (CRBP)-1 mRNA, whose translation pr...
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Calorie-related rapid onset of alveolar loss, regeneration, and changes in mouse lung gene expression
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Donald Massaro, Gloria Decarlo Massaro, Alexander S. Baras, Eric P. Hoffman, Linda Biadasz ClerchAbstract:Calorie restriction, followed by ad libitum refeeding, results, respectively, in loss and regeneration of Pulmonary Alveoli. We now show 35% of Alveoli are lost within 72 h of onset of calorie rest...
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Lung retinol storing cells synthesize and secrete retinoic acid, an inducer of alveolus formation
American Journal of Physiology-lung Cellular and Molecular Physiology, 2003Co-Authors: Ghenima Dirami, Gloria Decarlo Massaro, Linda Biadasz Clerch, Una S. Ryan, Peter R. Reczek, Donald MassaroAbstract:Retinoids play a key role in the formation of Pulmonary Alveoli. Lipid interstitial cells (LICs) of the alveolar wall store retinol and are concentrated at sites of alveolus formation, suggesting t...
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retinoic acid receptor β an endogenous inhibitor of the perinatal formation of Pulmonary Alveoli
Physiological Genomics, 2000Co-Authors: Gloria Decarlo Massaro, Donald Massaro, Waiyee Chan, Linda Biadasz Clerch, Norbert B Ghyselinck, Pierre Chambon, Roshantha A S ChandraratnaAbstract:Pulmonary Alveoli are formed, in part, by subdivision (septation) of the gas-exchange saccules of the immature lung. Septation is developmentally regulated, and failure to septate at the appropriate time is not followed by delayed spontaneous septation. We report retinoic acid receptor (RAR) β knockout mice exhibit premature septation; in addition, they form Alveoli twice as fast as wild-type mice during the period of septation but at the same rate as wild-type mice thereafter. Consistent with the perinatal effect of RARβ knockout, RARβ agonist treatment of newborn rats impairs septation. These results 1 ) identify RARβ as the first recognized endogenous signaling that inhibits septation, 2 ) demonstrate premature onset of septation may be induced, and 3 ) show the molecular signaling regulating alveolus formation differs during and after the period of septation. Suppressing perinatal RARβ signaling by RARβ antagonists may offer a novel, nonsurgical, means of preventing, or remediating, failed septation in prematurely born children.
Shinichi Ohno - One of the best experts on this subject based on the ideXlab platform.
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dynamic ultrastructure of Pulmonary Alveoli of living mice under respiratory conditions
2016Co-Authors: Shinichi Ohno, Nobuo Terada, Yasuhisa FujiiAbstract:A morphological approach to cell dynamics is usually difficult, since routine preparative techniques for electron microscopy always induce artefacts due to cessation of blood supply into organs. The in vivo cryotechnique followed by the freeze-substitution method reduces such technical problems. It was applied for examining the Pulmonary Alveoli of BALB/c mice in vivo. The following ultrastructural features were revealed. (1) A surfactant layer provided a continuous covering to the alveolar epithelium. (2) Typical lamellar structures in large alveolar epithelial cells were rarely detected. (3) Circulating erythrocytes with various shapes were observed in branching blood capillaries. (4) The close association between erythrocytes and the endothelium was seen at the peripheral alveolar septum. Such ultrastructural arrangements may be appropriate for the physiological functions of the Pulmonary Alveoli, such as exchanges of gases or materials in vivo.
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dynamic ultrastructure of mouse Pulmonary Alveoli revealed by an in vivo cryotechnique in combination with freeze substitution
Journal of Anatomy, 2000Co-Authors: Ichiro Takayama, Nobuo Terada, Yasuhisa Fujii, Takeshi Baba, Hideho Ueda, Yasuko Kato, Shinichi OhnoAbstract:A morphological approach to cell dynamics is usually difficult, since routine preparative techniques for electron microscopy always induce artifacts due to cessation of the blood supply into organs. An in vivo cryotechnique followed by the freeze-substitution method probably reduces such problems. It was applied for examining the Pulmonary Alveoli of BALB/c mice in vivo. The following ultrastructural features were revealed. (1) A surfactant layer provided a continuous covering to the alveolar epithelium. (2) Pleural epithelial cells, alveolar cells and endothelial cells contained many small vesicles and pits. In the alveolar epithelium, they were often localised near microtubules. (3) Typical lamellar structures in large alveolar epithelial cells were rarely detected. (4) Circulating erythrocytes with various shapes were observed in branching blood capillaries. (5) A close association between erythrocytes and the endothelium was seen at the peripheral alveolar septum. Such ultrastructural arrangements may be appropriate for the physiological functions of the Pulmonary Alveoli, such as exchanges of gases or materials in vivo.
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Dynamic ultrastructure of mouse Pulmonary Alveoli revealed by an in vivo cryotechnique in combination with freeze‐substitution
Journal of Anatomy, 2000Co-Authors: Ichiro Takayama, Nobuo Terada, Yasuhisa Fujii, Takeshi Baba, Hideho Ueda, Yasuko Kato, Shinichi OhnoAbstract:A morphological approach to cell dynamics is usually difficult, since routine preparative techniques for electron microscopy always induce artifacts due to cessation of the blood supply into organs. An in vivo cryotechnique followed by the freeze-substitution method probably reduces such problems. It was applied for examining the Pulmonary Alveoli of BALB/c mice in vivo. The following ultrastructural features were revealed. (1) A surfactant layer provided a continuous covering to the alveolar epithelium. (2) Pleural epithelial cells, alveolar cells and endothelial cells contained many small vesicles and pits. In the alveolar epithelium, they were often localised near microtubules. (3) Typical lamellar structures in large alveolar epithelial cells were rarely detected. (4) Circulating erythrocytes with various shapes were observed in branching blood capillaries. (5) A close association between erythrocytes and the endothelium was seen at the peripheral alveolar septum. Such ultrastructural arrangements may be appropriate for the physiological functions of the Pulmonary Alveoli, such as exchanges of gases or materials in vivo.
Roshantha A S Chandraratna - One of the best experts on this subject based on the ideXlab platform.
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retinoic acid receptor β an endogenous inhibitor of the perinatal formation of Pulmonary Alveoli
Physiological Genomics, 2000Co-Authors: Gloria Decarlo Massaro, Donald Massaro, Waiyee Chan, Linda Biadasz Clerch, Norbert B Ghyselinck, Pierre Chambon, Roshantha A S ChandraratnaAbstract:Pulmonary Alveoli are formed, in part, by subdivision (septation) of the gas-exchange saccules of the immature lung. Septation is developmentally regulated, and failure to septate at the appropriate time is not followed by delayed spontaneous septation. We report retinoic acid receptor (RAR) β knockout mice exhibit premature septation; in addition, they form Alveoli twice as fast as wild-type mice during the period of septation but at the same rate as wild-type mice thereafter. Consistent with the perinatal effect of RARβ knockout, RARβ agonist treatment of newborn rats impairs septation. These results 1 ) identify RARβ as the first recognized endogenous signaling that inhibits septation, 2 ) demonstrate premature onset of septation may be induced, and 3 ) show the molecular signaling regulating alveolus formation differs during and after the period of septation. Suppressing perinatal RARβ signaling by RARβ antagonists may offer a novel, nonsurgical, means of preventing, or remediating, failed septation in prematurely born children.
