The Experts below are selected from a list of 15795 Experts worldwide ranked by ideXlab platform
David L. Hopkins - One of the best experts on this subject based on the ideXlab platform.
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Effect of repeated Pulsed Electric Field treatment on the quality of hot-boned beef loins and topsides
Meat science, 2015Co-Authors: Alaa El-din A. Bekhit, Via Suwandy, Alan Carne, Remy Van De Ven, David L. HopkinsAbstract:Tenderness, water loss, colour and lipid stability are important quality attributes of fresh meat. This study investigated the effect of repeated (1×, 2× or 3×) Pulsed Electric Field treatment (10 kV, 90 Hz, 20 μs) on the tenderness, purge loss, cooking loss, colour, lipid oxidation, protein profile and post-treatment proteolysis of cold-boned beef longissimus lumborum and Musculus semimembranosus muscles. The shear force of beef longissimus lumborum was found to decrease by 2.5 N with every extra application of Pulsed Electric Field treatment, while the shear force of M. semimembranosus muscle was not significantly affected by Pulsed Electric Field treatment. There was an increase in proteolysis of beef longissimus lumborum treated with 1× Pulsed Electric Field as evident by increased troponin T and desmin degradation; however, less degradation was found with increasing number of Pulsed Electric Field treatments suggesting another mechanism such as physical disruption is responsible for the tenderisation of beef by Pulsed Electric Field.
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Effect of Repeated Pulsed Electric Field Treatment on the Quality of Cold-Boned Beef Loins and Topsides
Food and Bioprocess Technology, 2015Co-Authors: Via Suwandy, Alaa El-din A. Bekhit, Alan Carne, Remy Ven, David L. HopkinsAbstract:Tenderness, water loss, colour and lipid stability are important quality attributes of fresh meat. This study investigated the effect of repeated (1×, 2× or 3×) Pulsed Electric Field treatment (10 kV, 90 Hz, 20 μs) on the tenderness, purge loss, cooking loss, colour, lipid oxidation, protein profile and post-treatment proteolysis of cold-boned beef longissimus lumborum and Musculus semimembranosus muscles. The shear force of beef longissimus lumborum was found to decrease by 2.5 N with every extra application of Pulsed Electric Field treatment, while the shear force of M. semimembranosus muscle was not significantly affected by Pulsed Electric Field treatment. There was an increase in proteolysis of beef longissimus lumborum treated with 1× Pulsed Electric Field as evident by increased troponin T and desmin degradation; however, less degradation was found with increasing number of Pulsed Electric Field treatments suggesting another mechanism such as physical disruption is responsible for the tenderisation of beef by Pulsed Electric Field.
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Effect of Pulsed Electric Field treatment on hot-boned muscles of different potential tenderness
Meat science, 2015Co-Authors: Via Suwandy, Alaa El-din A. Bekhit, Alan Carne, Remy Van De Ven, David L. HopkinsAbstract:In this study, the effect of Pulsed Electric Field (PEF) treatment and ageing on the quality of beef M. longissimus lumborum (LL) and M. semimembranosus (SM) muscles was evaluated, including the tenderness, water loss and post-mortem proteolysis. Muscles were obtained from 12 steers (6 steers for each muscle), removed from the carcasses 4 hour postmortem and were treated with Pulsed Electric Field within 2h. Six different Pulsed Electric Field intensities (voltages of 5 and 10 kV × frequencies of 20, 50 and 90 Hz) plus a control were applied to each muscle to determine the optimum treatment conditions. Beef LL was found to get tougher with increasing treatment frequency whereas beef SM muscle was found to have up to 21.6% reduction in the shear force with Pulsed Electric Field treatment. Post-mortem proteolysis showed an increase in both troponin and desmin degradation in beef LL treated with low intensity PEF treatment (20 Hz) compared to non-treated control samples.
Howard Q Zhang - One of the best experts on this subject based on the ideXlab platform.
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commercial scale Pulsed Electric Field processing of tomato juice
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Seacheol Min, Tony Z Jin, Howard Q ZhangAbstract:Effects of commercial scale Pulsed Electric Field (PEF) processing on the quality of tomato juice were studied and compared with those of thermal processing. Tomato juice was prepared by hot break at 88 degrees C for 2 min or by cold break at 68 degrees C for 2 min and then thermally processed at 92 degrees C for 90 s or PEF processed at 40 kV/cm for 57 micros. Thermally processed, PEF processed, and unprocessed control juices were packed into 50 mL sterilized polypropylene tubes in a sanitary glovebox and stored at 4 degrees C for 112 days. Both thermally and PEF processed juices showed microbial shelf life at 4 degrees C for 112 days. The lipoxygenase activities of thermally and PEF processed juices were 0 and 47%, respectively. PEF processed juice retained more ascorbic acid than thermally processed juice at 4 degrees C for 42 days (p 0.05). Sensory evaluations indicated that flavor and overall acceptability of PEF processed juice were preferred to those of thermally processed juice (p < 0.05).
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Pulsed Electric Field processing effects on flavor compounds and microorganisms of orange juice
Food Chemistry, 1999Co-Authors: Mingyu Jia, Howard Q Zhang, David B MinAbstract:Abstract The headspace flavor compounds of fresh squeezed orange juice processed by Pulsed Electric Field (PEF) at 30 kV/cm for 240 or 480 μs, or heat at 90°C for 1 min were isolated by a solid phase microextraction (SPME) coating and separated by gas chromatography. The average losses of flavor compounds in orange juice processed by 240, 480 μs PEF and heat process were 3.0%, 9.0% and 22.0%, respectively ( P
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inactivation of listeria monocytogenes in milk by Pulsed Electric Field
Journal of Food Protection, 1998Co-Authors: Laura D Reina, Howard Q Zhang, Tony Z Jin, Ahmed E YousefAbstract:Pasteurized whole, 2%, and skim milk were inoculated with Listeria monocytogenes Scott A and treated with high-voltage Pulsed Electric Field (PEF). The effects of milk composition (fat content) and PEF parameters (Electric Field strength, treatment time, and treatment temperature) on the inactivation of the bacterium were studied. No significant differences were observed in the inactivation of L. monocytogenes Scott A in three types of milk by PEF treatment. With treatment at 25 degrees C, 1- to 3-log reductions of L. monocytogenes were observed. PEF lethal effect was a function of Field strength and treatment time. Higher Field strength or longer treatment time resulted in a greater reduction of viable cells. A 4-log reduction of the bacterium was obtained by increasing the treatment temperature to 50 degrees C. Results indicate that the use of a high-voltage PEF is a promising technology for inactivation of foodborne pathogens.
Darrin Uecker - One of the best experts on this subject based on the ideXlab platform.
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Safety and Efficacy of Nanosecond Pulsed Electric Field Treatment of Sebaceous Gland Hyperplasia.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2020Co-Authors: Girish S. Munavalli, Brian D. Zelickson, Mona M. Selim, Suzanne L. Kilmer, Thomas E. Rohrer, James P. Newman, Lauren Jauregui, William A Knape, Edward Ebbers, Darrin UeckerAbstract:Background Nanosecond Pulsed Electric Field (nsPEF) technology involves delivery of ultrashort pulses of Electrical energy and is a nonthermal, drug-free technology that has demonstrated favorable effects on cellular structures of the dermis and epidermis. Objective Determine the tolerability and effectiveness of nsPEF treatment of sebaceous gland hyperplasia (SGH). Methods This study was a prospective, randomized, open-label, multisite, nonsignificant risk trial in which each subject served as their own control. After injection of local anesthetic, high-intensity, ultrashort pulses of Electrical energy were used to treat 72 subjects resulting in a total of 222 treated lesions. Subjects returned for 3 to 4 follow-up evaluations with photographs. Results At the final study visit, 99.6% of treated SGH lesions were rated clear or mostly clear and 79.3% of the subjects were satisfied or mostly satisfied with the outcome. At 60 days after nsPEF treatment, 55% of the lesions were judged to have no hyperpigmentation and 31% exhibited mild post-treatment hyperpigmentation. At the last observation for all lesions, 32% of the 222 lesions were noted as having slight volume loss. Conclusion Nanosecond Pulsed Electric Field procedure is well tolerated and is very effective in the removal of SGHs. Trial registration ClinicalTrials.gov NCT03612570.
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safety and efficacy of nanosecond Pulsed Electric Field treatment of sebaceous gland hyperplasia
Dermatologic Surgery, 2019Co-Authors: Girish S. Munavalli, Brian D. Zelickson, Mona M. Selim, Suzanne L. Kilmer, Thomas E. Rohrer, James P. Newman, Lauren Jauregui, William A Knape, Edward Ebbers, Darrin UeckerAbstract:BACKGROUNDNanosecond Pulsed Electric Field (nsPEF) technology involves delivery of ultrashort pulses of Electrical energy and is a nonthermal, drug-free technology that has demonstrated favorable effects on cellular structures of the dermis and epidermis.OBJECTIVEDetermine the tolerability and effec
Dorota Witrowa-rajchert - One of the best experts on this subject based on the ideXlab platform.
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Acoustic and mechanical properties of carrot tissue treated by Pulsed Electric Field, ultrasound and combination of both
Journal of Food Engineering, 2018Co-Authors: Artur Wiktor, Ewa Gondek, Ewa Jakubczyk, Magdalena Dadan, Malgorzata Nowacka, Katarzyna Rybak, Dorota Witrowa-rajchertAbstract:Abstract: Both ultrasound (US) and Pulsed Electric Field treatment (PEF) allow achieving similar technological aims. Their application can for instance improve food preservation or enhance heat and mass transfer based processes. However, the mechanisms of action and the impact of these technologies on the structure of biological systems are different. Based on the knowledge concerning the behaviour of tissue subjected to Pulsed Electric Field or sonication it can be assumed that the combination of these techniques can be beneficial. Therefore the aim of this study was to analyse the impact of Pulsed Electric Field, ultrasound and combined (Pulsed Electric Field followed by ultrasound or ultrasound followed by Pulsed Electric Field) treatment on Electrical conductivity, intercellular structure, mechanical and acoustic properties of plant tissue as exemplified by carrot samples. Performed research proved that the mechanism of action and the consequences of US treatment are different in comparison to Pulsed Electric Field application. The efficiency of sonication cannot be evaluated on the basis of Electrical conductivity like it is usually done in the case of electroporation efficacy assessment. The results concerning mechanical and acoustic properties also indicate that Pulsed Electric Field causes higher alterations of intercellular structure than ultrasound. In general the utilization of US prior to PEF treatment can enhance the effectiveness of electroporation.
Girish S. Munavalli - One of the best experts on this subject based on the ideXlab platform.
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Safety and Efficacy of Nanosecond Pulsed Electric Field Treatment of Sebaceous Gland Hyperplasia.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 2020Co-Authors: Girish S. Munavalli, Brian D. Zelickson, Mona M. Selim, Suzanne L. Kilmer, Thomas E. Rohrer, James P. Newman, Lauren Jauregui, William A Knape, Edward Ebbers, Darrin UeckerAbstract:Background Nanosecond Pulsed Electric Field (nsPEF) technology involves delivery of ultrashort pulses of Electrical energy and is a nonthermal, drug-free technology that has demonstrated favorable effects on cellular structures of the dermis and epidermis. Objective Determine the tolerability and effectiveness of nsPEF treatment of sebaceous gland hyperplasia (SGH). Methods This study was a prospective, randomized, open-label, multisite, nonsignificant risk trial in which each subject served as their own control. After injection of local anesthetic, high-intensity, ultrashort pulses of Electrical energy were used to treat 72 subjects resulting in a total of 222 treated lesions. Subjects returned for 3 to 4 follow-up evaluations with photographs. Results At the final study visit, 99.6% of treated SGH lesions were rated clear or mostly clear and 79.3% of the subjects were satisfied or mostly satisfied with the outcome. At 60 days after nsPEF treatment, 55% of the lesions were judged to have no hyperpigmentation and 31% exhibited mild post-treatment hyperpigmentation. At the last observation for all lesions, 32% of the 222 lesions were noted as having slight volume loss. Conclusion Nanosecond Pulsed Electric Field procedure is well tolerated and is very effective in the removal of SGHs. Trial registration ClinicalTrials.gov NCT03612570.
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safety and efficacy of nanosecond Pulsed Electric Field treatment of sebaceous gland hyperplasia
Dermatologic Surgery, 2019Co-Authors: Girish S. Munavalli, Brian D. Zelickson, Mona M. Selim, Suzanne L. Kilmer, Thomas E. Rohrer, James P. Newman, Lauren Jauregui, William A Knape, Edward Ebbers, Darrin UeckerAbstract:BACKGROUNDNanosecond Pulsed Electric Field (nsPEF) technology involves delivery of ultrashort pulses of Electrical energy and is a nonthermal, drug-free technology that has demonstrated favorable effects on cellular structures of the dermis and epidermis.OBJECTIVEDetermine the tolerability and effec
