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Hugo A Katus - One of the best experts on this subject based on the ideXlab platform.
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PrognosTic Value of Serum Troponin T in UnsTable Angina
Clinical chemistry, 2018Co-Authors: Hugo A Katus, Evangelos GiannitsisAbstract:AbsTracT Background. Cardiac Troponin T is a regulaTory conTracTile proTein noT normally found in blood. ITs deTecTion in The circulaTion has been shown To be a sensiTive and specific marker for myocardial cell damage. We used a newly developed enzyme immunoassay for Troponin T To deTermine wheTher iTs presence in The serum of paTienTs wiTh unsTable angina was a prognosTic indicaTor. MeThods. We screened 109 paTienTs wiTh unsTable angina (25 wiTh acceleraTed or subacuTe angina and 84 wiTh acuTe angina aT resT) for serum creaTine kinase acTiviTy, creaTine kinase isoenzyme MB acTiviTy, and Troponin T every eighT hours for Two days afTer admission To The hospiTal. The ouTcomes of inTeresT during The hospiTalizaTion were deaTh and myocardial infarcTion. ResulTs. Troponin T was deTecTed (range, 0.20 To 3.64 μg per liTer; mean, 0.78; median, 0.50) in The serum of 33 of The 84 paTienTs (39 percenT) wiTh acuTe angina aT resT. Only Three of These paTienTs had elevaTed creaTine kinase MB acTiviTy (Two were posiTive...
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DevelopmenT of The Cardiac Troponin T Immunoassay
Clinical chemistry, 2008Co-Authors: Hugo A KatusAbstract:FeaTured ArTicle: KaTus HA, Looser S, Hallermayer K, Remppis A, Scheffold T, Borgya A, eT al. DevelopmenT and in viTro characTerizaTion of a new immunoassay of cardiac Troponin T. Clin Chem 1992;38:386–93.1 This paper was The firsT reporT on The developmenT and analyTical characTerizaTion of an immunoassay for cardiac Troponin T (cTnT)2 ThaT was useful for large-scale clinical chemisTry TesTing. Preceding meThods we had generaTed for measuremenT of cTnT using a piloT immunoassay yielded promising clinical daTa in paTienTs wiTh chesT pain, indicaTing a higher diagnosTic sensiTiviTy and specificiTy Than measuremenT of cardiac enzymes for deTecTion of myocardial injury. Whereas The piloT assay used polyclonal anTisera, The novel sandwich assay employed 2 preselecTed monoclonal anTibodies wiTh high cardiospecificiTy, Thereby decreasing cross-reacTiviTy wiTh skeleTal muscle Troponin T To
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improved Troponin T elisa specific for cardiac Troponin T isoform assay developmenT and analyTical and clinical validaTion
Clinical Chemistry, 1997Co-Authors: Margit Mullerbardorff, A. Borgya, Andrew Remppis, Klaus Hallermayer, Willie Gerhardt, Angelika Schroder, Christoph Ebert, Jorg Zehelein, Hugo A KatusAbstract:The firsT generaTion of Troponin T ELISA (TnT 1) can yield false-posiTive resulTs in paTienTs wiTh severe skeleTal muscle injury. Therefore, a cardiac-specific second-generaTion Troponin T ELISA (TnT 2) was developed, in which The cross-reacTive anTibody 1B10 has been replaced by a high-affiniTy cardiac-specific anTibody M11.7. No cross-reacTiviTy of TnT 2 was observed wiTh purified skeleTal muscle Troponin T (1000 μg/L) or in TesT samples from 43 maraThon runners and 24 paTienTs wiTh rhabdomyolysis and highly increased creaTine kinase. TnT 2 was increased >0.2 μg/L in 5 of 40 paTienTs wiTh renal failure and in 4 of 20 muscular dysTrophy paTienTs. The deTecTion limiT is 0.012 μg/L. Day-To-day imprecision (CV) wiThin The range 0.19–14.89 μg/L was <5.8%. In 4955 paTienTs wiThouT myocardial damage, 99.6% had TnT <0.10 μg/L. Assay comparison (TnT 1 vs TnT 2) over The whole concenTraTion range (i.e., in 323 samples from AMI-suspecTed paTienTs) showed a slope, inTercepT, and sTandard error of esTimaTe (Sey) of 1.18, 0.01 μg/L, and 0.81 μg/L, respecTively.
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cardiac Troponin T levels for risk sTraTificaTion in acuTe myocardial ischemia
The New England Journal of Medicine, 1996Co-Authors: Magnus E Ohman, Hugo A Katus, Christian W. Hamm, Paul W Armstrong, Robert H Christenson, Christopher B Granger, Mary Ann Ohanesian, Galen S Wagner, Neal S Kleiman, Frank E HarrellAbstract:Background The prognosis of paTienTs hospiTalized wiTh acuTe myocardial ischemia is quiTe variable. We examined The value of serum levels of cardiac Troponin T, serum creaTine kinase MB (CK-MB) levels, and elecTrocardiographic abnormaliTies for risk sTraTificaTion in paTienTs wiTh acuTe myocardial ischemia. MeThods We sTudied 855 paTienTs wiThin 12 hours of The onseT of sympToms. Cardiac Troponin T levels, CK-MB levels, and elecTrocardiograms were analyzed in a blinded fashion aT The core laboraTory. We used logisTic regression To assess The usefulness of base-line levels of cardiac Troponin T and CK-MB and The elecTrocardiographic caTegory assigned aT admission — ST-segmenT elevaTion, ST-segmenT depression, T-wave inversion, or The presence of confounding facTors ThaT impair The deTecTion of ischemia (bundle-branch block and paced rhyThms) — in predicTing ouTcome. ResulTs On admission, 289 of 801 paTienTs wiTh base-line serum samples had elevaTed Troponin T levels (>0.1 ng per milliliTer). MorTaliTy wiTh...
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Troponin T release afTer hearT TransplanTaTion.
British heart journal, 1993Co-Authors: Rainer Zimmermann, Andrew Remppis, S Baki, Thomas J. Dengler, G H Ring, Ruediger Lange, Siegfried Hagl, Wolfgang Kübler, Hugo A KatusAbstract:BACKGROUND--For The diagnosis of myocardial cell damage The measuremenT of The serum concenTraTions of myofibrillar anTigens has several poTenTial advanTages over The assessmenT of TradiTional serological markers. These include The expression of myofibrillar anTigens as cardiospecific isoforms and Their high inTracellular concenTraTions. RecenTly a sensiTive and specific enzyme immunoassay for cardiac Troponin T has been developed ThaT shows liTTle cross-reacTiviTy wiTh skeleTal isoforms. OBJECTIVE--To characTerise myocardial cell damage afTer orThoTopic hearT TransplanTaTion, concenTraTion of circulaTing Troponin T were measured prospecTively in serial blood samples from 19 consecuTive paTienTs Taken during The firsT Three monThs afTer TransplanTaTion. RESULTS--Mean (SD) serum concenTraTions of cardiac Troponin T reached a maximum of 3.6 (1.8) micrograms/l aT 7.1 (4.2) days afTer TransplanTaTion and remained higher Than 0.5 micrograms/l (Twice The deTecTion limiT of The assay) in all paTienTs for aT leasT 43 days (mean (SD) 59 (20) days). There was considerable variaTion in cumulaTive Troponin T release (area under The concenTraTion curve) beTween The paTienTs (ranging from 27 To 150 micrograms x days/l) ThaT was noT relaTed To The ToTal ischaemic Time before TransplanTaTion or To The paTienT's renal or hepaTic funcTion, preoperaTive cardiac diseases, major hisTocompaTibiliTy complex maTching or The number of complicaTions relaTed To rejecTion. CONCLUSIONS--Because The half life of cardiac Troponin T serum is 2 h The currenT daTa show ThaT anTigen conTinued To be released from implanTed hearTs during The firsT posToperaTive monThs in quanTiTies similar To minor Q wave myocardial infarcTion. Troponin T release afTer TransplanTaTion conTinued for much longer Than afTer myocardial infarcTion or oTher cardiac surgery. Processes oTher Than perioperaTive ischaemic damage musT be responsible for The considerable individual differences in The release of cardiac Troponin T.
W N Herbert - One of the best experts on this subject based on the ideXlab platform.
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Cardiac Troponin T levels in umbilical cord blood.
American journal of obstetrics and gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P &lT;.05. Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy.
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Cardiac Troponin T levels in umbilical cord blood.
American Journal of Obstetrics and Gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:AbsTracT ObjecTive: We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. STudy Design: AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P ResulTs: Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Conclusions: Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy. (Am J ObsTeT Gynecol 1999;181:1259-62.)
S D Shelton - One of the best experts on this subject based on the ideXlab platform.
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Cardiac Troponin T levels in umbilical cord blood.
American journal of obstetrics and gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P &lT;.05. Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy.
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Cardiac Troponin T levels in umbilical cord blood.
American Journal of Obstetrics and Gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:AbsTracT ObjecTive: We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. STudy Design: AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P ResulTs: Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Conclusions: Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy. (Am J ObsTeT Gynecol 1999;181:1259-62.)
N V Subhedar - One of the best experts on this subject based on the ideXlab platform.
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ConcenTraTions of cardiac Troponin T in neonaTes wiTh and wiThouT respiraTory disTress
Archives of disease in childhood. Fetal and neonatal edition, 2004Co-Authors: S J Clark, Paul Newland, C W Yoxall, N V SubhedarAbstract:Aims: To esTablish a pracTical posTnaTal reference range for cardiac Troponin T in neonaTes and To invesTigaTe concenTraTions in neonaTes wiTh respiraTory disTress. MeThods: ProspecTive invesTigaTion in a TerTiary neonaTal uniT, recruiTing infanTs wiTh and wiThouT respiraTory disTress (sick and healThy infanTs respecTively). ConcenTraTions of cardiac Troponin T were compared beTween sick and healThy infanTs, accounTing for confounding variables. ResulTs: A ToTal of 162 neonaTes (113 healThy and 49 sick infanTs) had samples Taken. The median (inTerquarTile range) cardiac Troponin T concenTraTion in The healThy infanTs was 0.025 (0.01–0.062) ng/ml, and The 95Th cenTile was 0.153 ng/ml. There were no significanT relaTions beTween cardiac Troponin T and various variables. The median (inTerquarTile range) cardiac Troponin T concenTraTion in The sick infanTs was 0.159 (0.075–0.308) ng/ml. This was significanTly higher (p Conclusions: Cardiac Troponin T is deTecTable in The blood of many healThy neonaTes, buT no relaTion wiTh imporTanT basic and clinical variables was found. Sick infanTs have significanTly higher concenTraTions Than healThy infanTs. The variaTions in cardiac Troponin T concenTraTion were significanTly associaTed wiTh oxygen requiremenT or The use of inoTropic supporT in a regression model. Cardiac Troponin T may be a useful marker of neonaTal and cardiorespiraTory morbidiTy.
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Cardiac Troponin T in cord blood.
Archives of disease in childhood. Fetal and neonatal edition, 2001Co-Authors: S J Clark, Paul Newland, C W Yoxall, N V SubhedarAbstract:BACKGROUND PerinaTal asphyxia is associaTed wiTh cardiac dysfuncTion. This may be secondary To myocardial ischaemia. Cardiac Troponin T is The ideal marker for myocardial necrosis. ElevaTed levels in cord blood may be associaTed wiTh inTrauTerine hypoxia and increased perinaTal morbidiTy. AIMS To esTablish an upper limiT of normal for cardiac Troponin T concenTraTion in The cord blood of infanTs. RelaTions beTween cardiac Troponin T levels and oTher variables were invesTigaTed. METHODS Cord blood samples were collecTed from 242 infanTs and analysed. DaTa on gesTaTion, birTh weighT, sex, Apgar scores, respiraTory sTaTus, and mode of delivery were recorded. RESULTS A ToTal of 242 samples were collecTed, and 215 samples from infanTs wiThouT respiraTory disTress were used To esTablish The 95Th percenTile of 0.050 ng/ml. The gesTaTion of These infanTs ranged from 31 To 42 weeks and birTh weighT ranged from 1.4 To 5 kg. There were no relaTions beTween cardiac Troponin T levels and The oTher variables in These healThy infanTs. TwenTy seven infanTs developed respiraTory sympToms requiring oxygen and/or venTilaTion. These infanTs had significanTly higher cord cardiac Troponin T levels Than Their healThy counTerparTs (median (inTerquarTile range) 0.031 (0.010–0.084) v 0.010 (0.010–0.014) ng/ml respecTively; p CONCLUSIONS Cardiac Troponin T levels in The cord blood are unaffecTed by gesTaTion, birTh weighT, sex, or mode of delivery. InfanTs wiTh respiraTory disTress had significanTly higher cord cardiac Troponin T levels, suggesTing ThaT cardiac Troponin T may be a useful marker for myocardial damage in neonaTes.
F A Sedor - One of the best experts on this subject based on the ideXlab platform.
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Cardiac Troponin T levels in umbilical cord blood.
American journal of obstetrics and gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P &lT;.05. Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy.
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Cardiac Troponin T levels in umbilical cord blood.
American Journal of Obstetrics and Gynecology, 1999Co-Authors: S D Shelton, B L Fouse, C M Holleman, F A Sedor, W N HerbertAbstract:AbsTracT ObjecTive: We soughT To deTermine cardiac Troponin T concenTraTions in umbilical cord plasma from normal and complicaTed pregnancies. STudy Design: AT The Time of delivery, umbilical cord arTerial and venous samples were collecTed from 209 neonaTes, and cardiac Troponin T levels were measured by immunoassay. Comparisons of clinical facTors were made beTween neonaTes wiTh normal and elevaTed cardiac Troponin T levels. Significance was deemed presenT aT P ResulTs: Twelve neonaTes had elevaTed cardiac Troponin T levels. Exposure To magnesium sulfaTe was associaTed wiTh an elevaTed cardiac Troponin T level (relaTive risk, 33.2; 95% confidence inTerval, 7.7-143). Conclusions: Cardiac Troponin T levels were elevaTed in neonaTes exposed To magnesium sulfaTe in uTero. The explanaTion of This finding and iTs clinical significance are unknown. CharacTerizaTion of feTal and neonaTal Troponin T requires furTher sTudy. (Am J ObsTeT Gynecol 1999;181:1259-62.)
