The Experts below are selected from a list of 90 Experts worldwide ranked by ideXlab platform
W. Jessie Suneetha - One of the best experts on this subject based on the ideXlab platform.
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Effect of Germination on Vitamin C Content and Amylolytic Activity of Quinoa (Chenopodium Quinoa Willd)
International Research Journal of Pure and Applied Chemistry, 2020Co-Authors: M. Naga Sai Srujana, B. Anila Kumari, K. Uma Maheswari, K.b. Suneetha Devi, W. Jessie SuneethaAbstract:Aims: To study the parameters for recording speed of germination, vitamin C and amylolytic changes in Quinoa over a period of 72 h. Sample: Whole (HGQ) Quinoa Grain (Chenopodium Quinoa Willd) was procured from Department of Agronomy, College of Agriculture, PJTS Agricultural University, Rajendranagar, Hyderabad and commercially processed Quinoa seed (CGQ) was purchased from local market was studied for the germination capacity after dehulling. Study Design: Analyzing the germination speed parameters and vitamin C, amylolytic changes over 72 h of time period. Place and Duration of Study: Analysis was conducted in PGRC Laboratory, PJTSAU, Rajendranagar, Hyderabad. Results: Quinoa has strong germination capacity. The result indicates that higher FGP was seen at 20°C (83%) followed by 15°C (81%) and 25°C (80%). The radicals protruded after 4hrs of imbibition and reached to maximum length at 68hrs for 15°C and 25°C, where as it continued up to 72hrs for 20°C. Box plot diagram showed the median values at 20°C temperature ranged between 0.95 to 36.95 where as at 15°C it was 0.41 to 29.1 and 0.69 to 33.55 in 25°C indicating maximum viability of the Grain at 20°C temperature. The mean scores of vitamin C content was high at 20°C temperature at all stages when compared to other two temperatures i.e. 15°C and 25°C. It showed that α- amylase activity was developed in germinated samples and rapidly increased up to 32hrs of imbibition and slightly decreased after 48hrs of germination. Conclusion: Quinoa has high germinated capacity as it started from 4hrs upon germination.
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Antioxidant and Anti Nutritional Composition of Germinated Quinoa (Chenopodium Quinoa Willd)
Current Journal of Applied Science and Technology, 2020Co-Authors: M. Naga Sai Srujana, B. Anila Kumari, K. Uma Maheswari, K.b. Suneetha Devi, W. Jessie SuneethaAbstract:Aim: To analyse the antioxidant and antinutritional components of germinated Quinoa. Sample: Whole (HGQ) and dehulled (DGQ) Quinoa Grain (Chenopodium Quinoa Willd) was procured from Department of Agronomy, College of Agriculture, PJTS Agricultural University, Rajendranagar, Hyderabad and commercially processed Quinoa seed (CGQ) purchased from local market was germinated at 20ºC temperature for 4hrs and analyzed. Study Design: Analysing antioxidants and antinutritional components. Place and Duration of Study: Analysis was conducted in PGRC Laboratory, PJTSAU, Rajendranagar, Hyderabad. Results: The total phenol content and flavonoid content increased upon germination and was high for HGQ and DGQ was high compared to CGQ. IC50 value for antioxidant value of CGQ in methanol and water extract was low compared to HGQ and DGQ. The oxalate content of germinated Quinoa ranged from 6.17 ±0.01 to 9.45±0.02mg/100g. The saponin content of germinated Quinoa was high for HGQ and DGQ compared to CGQ. When compared to the raw there was 104.16% reduction was seen in HGQ, whereas it reduced to 404.85% in DGQ. But when compared to the commercial variety the saponin contents were 23.07% (DGQ) and 69.35% (HGQ) more. This may be because of processing conditions of commercial variety.
D. J. Fairbanks - One of the best experts on this subject based on the ideXlab platform.
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Construction of a Quinoa (Chenopodium Quinoa Willd.) BAC library and its use in identifying genes encoding seed storage proteins
Theoretical and Applied Genetics, 2006Co-Authors: M. R. Stevens, C. E. Coleman, S. E. Parkinson, P. J. Maughan, H.-b. Zhang, M. R. Balzotti, D. L. Kooyman, K. Arumuganathan, A. Bonifacio, D. J. FairbanksAbstract:Quinoa ( Chenopodium Quinoa Willd.) is adapted to the harsh environments of the Andean Altiplano region. Its seeds have a well-balanced amino acid composition and exceptionally high protein content with respect to human nutrition. Quinoa Grain is a staple in the diet of some of the most impoverished people in the world. The plant is an allotetraploid displaying disomic inheritance (2 n =4 x =36) with a di-haploid genome of 967 Mbp (megabase pair), or 2 C =2.01 pg. We constructed two Quinoa BAC libraries using Bam HI (26,880 clones) and Eco RI (48,000 clones) restriction endonucleases. Cloned inserts in the Bam HI library average 113 kb (kilobase) with approximately 2% of the clones lacking inserts, whereas cloned inserts in the Eco RI library average 130 kb and approximately 1% lack inserts. Three plastid genes used as probes of high-density arrayed blots of 73,728 BACs identified approximately 2.8% of the clones as containing plastid DNA inserts. We estimate that the combined Quinoa libraries represent at least 9.0 di-haploid nuclear genome equivalents. An average of 12.2 positive clones per probe were identified with 13 Quinoa single-copy ESTs as probes of the high-density arrayed blots, suggesting that the estimate of 9.0× coverage of the genome is conservative. Utility of the BAC libraries for gene identification was demonstrated by probing the library with a partial sequence of the 11S globulin seed storage protein gene and identifying multiple positive clones. The presence of the 11S globulin gene in four of the clones was verified by direct comparison with Quinoa genomic DNA on a Southern blot. Besides serving as a useful tool for gene identification, the Quinoa BAC libraries will be an important resource for physical mapping of the Quinoa genome.
H. Knoblochová - One of the best experts on this subject based on the ideXlab platform.
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Extention of the spectra of plant products for the diet in coeliac disease
Czech Journal of Food Sciences, 2018Co-Authors: J. Petr, I. Michalík, H. Tlaskalová, I. Capouchová, D. Urminská, L. Tučková, Oldrich Famera, H. KnoblochováAbstract:The authors studied an extension of the sources of plant products for the diet in coeliac disease. This disease is induced by the components of glutenin proteins. In a collection of crops, they examined the contents of the total and protein nitrogen, the composition of protein fractions, the electrophoretic composition of reserve gluten and prolamine proteins, and the immunological determination of the gliadin amount using ELISA test. By immunological tests, gliadin content below 10 mg per 100 g of sample was found in the following species: amaranth (Amaranthus hypochondriacus and A. cruentus) followed by Quinoa (Chenopodium Quinoa), sorghum species - Grain sorghum and sweet sorghum (Sorghum bicolor and S. saccharatum), millet (Panicum miliaceum), foxtail millet (Setaria italica ssp. maxima), broadrood (Digitaria sanguinalis) and buckwheat (Fagapyrum esculentum). These species can be considered as suitable for the diet in coeliac disease. Below-limit values were found in triticale (Triticosccale) and some oats varieties; this, however, will need some other tests. The analysed samples differred by the contents of crude protein and fraction structures of the protein complex. In pseudocereals amaranth, Quinoa and buckwheat, the proportion of the soluble fractions of albumin and globulin was 50-65%. In Grain sorghum, their proportion was 20.5%, in sweet sorghum 7.8%. In millet, foxtail millet, and broadrood, their proportion amounted to 12-13%. The proportion of prolamines was higher in sweet sorghum than in Grain sorghum. Pseudocereals and millet contained 3-6% of prolamines, Italian millet 38.7%, and broadrood 23.1%, respectively. The two latter species had, however, lower contents of glutenins. In the other species studied, the contents of glutenins ranged from 12 to 22%. Electrophoretic analysis PAGE of prolamine proteins or SDS-PAGE ISTA, developed for gluten proteins, confirmed the results of immunological tests on the suitability of Quinoa, Grain sorghum, sweet sorghum, buckwheat, amaranth, broadrood, millet and foxtail millet for the diet in coeliac disease. These species did not contain prolamins or the content of α-prolamins was negligible in the given samples. The tested species of wheat, triticale, and oats species were manifested as substandard or unhealthy for the diet.
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Extention of the spectra of plant products for the diet in coeliac disease
Czech Journal of Food Sciences, 2011Co-Authors: J. Petr, I. Michalík, H. Tlaskalová, I. Capouchová, O. Faměra, D. Urminská, L. Tučková, H. KnoblochováAbstract:The authors studied an extension of the sources of plant products for the diet in coeliac disease. This disease is induced by the components of glutenin proteins. In a collection of crops, they examined the contents of the total and protein nitrogen, the composition of protein fractions, the electrophoretic composition of reserve gluten and prolamine proteins, and the immunological determination of the gliadin amount using ELISA test. By immunological tests, gliadin content below 10 mg per 100 g of sample was found in the following species: amaranth (Amaranthus hypochondriacus and A. cruentus) followed by Quinoa (Chenopodium Quinoa), sorghum species – Grain sorghum and sweet sorghum (Sorghum bicolor and S. saccharatum), millet (Panicum miliaceum), foxtail millet (Setaria italica ssp. maxima), broadrood (Digitaria sanguinalis) and buckwheat (Fagopyrum esculentum). These species can be considered as suitable for the diet in coeliac disease. Below-limit values were found in triticale (Triticosecale) and some oats varieties; this, however, will need some other tests. The analysed samples differred by the contents of crude protein and fraction structures of the protein complex. In pseudocereals amaranth, Quinoa and buckwheat, the proportion of the soluble fractions of albumin and globulin was 50–65%. In Grain sorghum, their proportion was 20.5%, in sweet sorghum 7.8%. In millet, foxtail millet, and broadrood, their proportion amounted to 12–13%. The proportion of prolamines was higher in sweet sorghum than in Grain sorghum. Pseudocereals and millet contained 3–6% of prolamines, Italian millet 38.7%, and broadrood 23.1%, respectively. The two latter species had, however, lower contents of glutenins. In the other species studied, the contents of glutenins ranged from 12 to 22%. Electrophoretic analysis PAGE of prolamine proteins or SDS-PAGE ISTA, developed for gluten proteins, confirmed the results of immunological tests on the suitability of Quinoa, Grain sorghum, sweet sorghum, buckwheat, amaranth, broadrood, millet and foxtail millet for the diet in coeliac disease. These species did not contain prolamins or the content of -prolamins was negligible in the given samples. The tested species of wheat, triticale, and oats species were manifested as substandard or unhealthy for the diet.
Ronei J Poppi - One of the best experts on this subject based on the ideXlab platform.
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direct analysis of the main chemical constituents in chenopodium Quinoa Grain using fourier transform near infrared spectroscopy
Food Control, 2015Co-Authors: Daniela Souza Ferreira, Juliana Azevedo Lima Pallone, Ronei J PoppiAbstract:Abstract Interest in the research and development of Quinoa, an indigenous Andean crop, has increased in the last 15 years, mainly because of its nutritional value and high seed yield. Near-infrared spectroscopy (NIRS) is a rapid non-destructive technique, which is useful for studying the chemical properties of these crops. Considering these advantages, the objective of this research is to develop NIRS calibrations suitable for the routine determination of dietary constituents in 78 varieties of Quinoa using partial least squares (PLS). For recording NIR a sample spinner accessory of diffuse reflectance was applied directly on the Quinoa samples without treatment was used. The PLS models developed for the quantification of moisture, ash, lipid, protein and carbohydrate content showed that the proposed methodology produced suitable results, with the graph of the real and predicted concentrations having a coefficient of determination ( R 2 ) > 0.737 and RMSEP
M. Naga Sai Srujana - One of the best experts on this subject based on the ideXlab platform.
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Effect of Germination on Vitamin C Content and Amylolytic Activity of Quinoa (Chenopodium Quinoa Willd)
International Research Journal of Pure and Applied Chemistry, 2020Co-Authors: M. Naga Sai Srujana, B. Anila Kumari, K. Uma Maheswari, K.b. Suneetha Devi, W. Jessie SuneethaAbstract:Aims: To study the parameters for recording speed of germination, vitamin C and amylolytic changes in Quinoa over a period of 72 h. Sample: Whole (HGQ) Quinoa Grain (Chenopodium Quinoa Willd) was procured from Department of Agronomy, College of Agriculture, PJTS Agricultural University, Rajendranagar, Hyderabad and commercially processed Quinoa seed (CGQ) was purchased from local market was studied for the germination capacity after dehulling. Study Design: Analyzing the germination speed parameters and vitamin C, amylolytic changes over 72 h of time period. Place and Duration of Study: Analysis was conducted in PGRC Laboratory, PJTSAU, Rajendranagar, Hyderabad. Results: Quinoa has strong germination capacity. The result indicates that higher FGP was seen at 20°C (83%) followed by 15°C (81%) and 25°C (80%). The radicals protruded after 4hrs of imbibition and reached to maximum length at 68hrs for 15°C and 25°C, where as it continued up to 72hrs for 20°C. Box plot diagram showed the median values at 20°C temperature ranged between 0.95 to 36.95 where as at 15°C it was 0.41 to 29.1 and 0.69 to 33.55 in 25°C indicating maximum viability of the Grain at 20°C temperature. The mean scores of vitamin C content was high at 20°C temperature at all stages when compared to other two temperatures i.e. 15°C and 25°C. It showed that α- amylase activity was developed in germinated samples and rapidly increased up to 32hrs of imbibition and slightly decreased after 48hrs of germination. Conclusion: Quinoa has high germinated capacity as it started from 4hrs upon germination.
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Antioxidant and Anti Nutritional Composition of Germinated Quinoa (Chenopodium Quinoa Willd)
Current Journal of Applied Science and Technology, 2020Co-Authors: M. Naga Sai Srujana, B. Anila Kumari, K. Uma Maheswari, K.b. Suneetha Devi, W. Jessie SuneethaAbstract:Aim: To analyse the antioxidant and antinutritional components of germinated Quinoa. Sample: Whole (HGQ) and dehulled (DGQ) Quinoa Grain (Chenopodium Quinoa Willd) was procured from Department of Agronomy, College of Agriculture, PJTS Agricultural University, Rajendranagar, Hyderabad and commercially processed Quinoa seed (CGQ) purchased from local market was germinated at 20ºC temperature for 4hrs and analyzed. Study Design: Analysing antioxidants and antinutritional components. Place and Duration of Study: Analysis was conducted in PGRC Laboratory, PJTSAU, Rajendranagar, Hyderabad. Results: The total phenol content and flavonoid content increased upon germination and was high for HGQ and DGQ was high compared to CGQ. IC50 value for antioxidant value of CGQ in methanol and water extract was low compared to HGQ and DGQ. The oxalate content of germinated Quinoa ranged from 6.17 ±0.01 to 9.45±0.02mg/100g. The saponin content of germinated Quinoa was high for HGQ and DGQ compared to CGQ. When compared to the raw there was 104.16% reduction was seen in HGQ, whereas it reduced to 404.85% in DGQ. But when compared to the commercial variety the saponin contents were 23.07% (DGQ) and 69.35% (HGQ) more. This may be because of processing conditions of commercial variety.
