The Experts below are selected from a list of 1716 Experts worldwide ranked by ideXlab platform
Zhengtao Wang - One of the best experts on this subject based on the ideXlab platform.
-
metabolomics study of anti inflammatory action of Radix Paeoniae rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography mass spectrometry
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Li Yang, Aizhen Xiong, Rui Wang, Lan Lu, Zhengtao WangAbstract:OBJECTIVE: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. METHOD: To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. CONCLUSION: The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
metabolomics study of anti platelet effect of Radix Paeoniae rubra and Radix Paeoniae Alba by uplc ms
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Li Yang, Aizhen Xiong, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. METHOD: After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. CONCLUSION: Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae Alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Rui Wang, Xuemei Cheng, Yuqi He, Cheng WuAbstract:Abstract An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69 ± 1.46 and 1.46 ± 0.29 (p 0.05) and a larger AUC (24.89 ± 7.41 μg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the tmax, Cmax, AUC, t1/2, CL, and Vd among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of tmax, Cmax, and AUC of paeoniflorin were remarkably increased (P
-
hplc fingerprinting of Radix Paeoniae Alba
Acta pharmaceutica Sinica, 2007Co-Authors: Liu Yang, Shunjun Xu, Runtao Tian, Zhengtao WangAbstract:: To establish a sensitive and specific HPLC method for quality control of Radix Paeoniae Alba, HPLC method was applied for quality assessment of Radix Paeoniae Alba. HPLC analysis was performed on a Symmetry C18 column (250 mm x 4. 6 mm ID, 5 microm, Waters, USA). The mobile phase consisted of acetonitrile (solvent A) and water containing 0.1% (v/v) phosphoric acid (solvent B) at a constant flow rate of 0.8 mL x min(-1). An increasing linear gradient (v/v) of solvent A was used (t/min, % A): (0,10), (5,10), (25,15), (45, 22), (46, 65), (50, 80) and (60, 80). The column temperature was set at 25 degrees C. The chromatograms were monitored at 230 nm and the on-line UV spectra were recorded in the range of 190 - 400 nm. The HPLC chromatographic fingerprinting of Radix Paeoniae Alba, showing 11 characteristic peaks, was established from 28 lots of Radix Paeoniae Alba. The areas of main chromatographic peaks were found to complied with the following rule: paeoniflorin > 1, 2, 3, 4, 6-penta-O-galloyl-glucos > albiflorin > methyl gallate > other compounds. The chromatographic fingerprinting of Radix Paeoniae Alba with high specificity can be used to control its quality and assure lot-to-lot consistency.
Rui Wang - One of the best experts on this subject based on the ideXlab platform.
-
Radix Paeoniae rubra and Radix Paeoniae Alba attenuate ccl4 induced acute liver injury an ultra performance liquid chromatography mass spectrometry uplc ms based metabolomic approach for the pharmacodynamic study of traditional chinese medicines tcms
International Journal of Molecular Sciences, 2012Co-Authors: Rui Wang, Qiwei Yang, Aizhen Xiong, Zhongqiu Teng, Lili YangAbstract:Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl4-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl4 injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
-
metabolomics study of anti platelet effect of Radix Paeoniae rubra and Radix Paeoniae Alba by uplc ms
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Li Yang, Aizhen Xiong, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. METHOD: After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. CONCLUSION: Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
metabolomics study of anti inflammatory action of Radix Paeoniae rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography mass spectrometry
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Li Yang, Aizhen Xiong, Rui Wang, Lan Lu, Zhengtao WangAbstract:OBJECTIVE: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. METHOD: To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. CONCLUSION: The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae Alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Rui Wang, Xuemei Cheng, Yuqi He, Cheng WuAbstract:Abstract An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69 ± 1.46 and 1.46 ± 0.29 (p 0.05) and a larger AUC (24.89 ± 7.41 μg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the tmax, Cmax, AUC, t1/2, CL, and Vd among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of tmax, Cmax, and AUC of paeoniflorin were remarkably increased (P
Jida Zhang - One of the best experts on this subject based on the ideXlab platform.
-
exploring potential chemical transformation by chemical profiling approach for rapidly evaluating chemical consistency between sun dried and sulfur fumigated Radix Paeoniae Alba using ultraperformance liquid chromatography coupled with time of flight
Evidence-based Complementary and Alternative Medicine, 2013Co-Authors: Jida ZhangAbstract:Ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-QTOF/MS) based on a chemical profiling method was applied to rapidly evaluate the chemical consistency between sun-dried and sulfur-fumigated Radix Paeoniae Alba. By virtue of the high resolution, high speed of UPLC, and the accurate mass measurement of TOFMS coupled with reliable MarkerLynx software, five newly assigned monoterpene glycoside sulfonates were found and identified in sulfur-fumigated Radix Paeoniae Alba samples. This method could be applied for rapid quality evaluation of different kinds of sulfur-fumigated Radix Paeoniae Alba among commercial samples.
Jinao Duan - One of the best experts on this subject based on the ideXlab platform.
-
simultaneous determination of bioactive components of Radix angelicae sinensis Radix Paeoniae Alba herb couple in rat plasma and tissues by uplc ms ms and its application to pharmacokinetics and tissue distribution
Journal of Chromatography B, 2014Co-Authors: Zhenhao Li, Dawei Qian, Yefei Qian, Jinao DuanAbstract:Abstract A highly sensitive and rapid ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) has been developed and validated for simultaneous quantification of seven components in rat plasma and five components in rat tissues after oral administration of the extracts of different combination Radix Angelicae Sinensis–Radix Paeoniae Alba herb couple and has been applied to compare the different pharmacokinetics and tissue distribution properties of these bioactive components. The extracts of Radix Angelicae Sinensis (RAS), Radix Paeoniae Alba (RPA) and Radix Angelicae Sinensis–Radix Paeoniae Alba herb couple (RRHC) were orally administrated to rats, respectively. The concentrations of ferulic acid, caffeic acid, vanillic acid, ligustilide, paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma and the concentrations of ferulic acid, vanillic acid, paeoniflorin, albiflorin and oxypaeoniflorin in tissues were determined by UPLC–MS/MS. The plasma samples were pretreated by protein precipitation with methanol and the tissue samples were homogenated with water and pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C 18 column using 0.1% formic acid–acetonitrile as mobile phase for gradient elution. A triple quadrupole (TQ) tandem mass spectrometry equipped with an electrospray ionization source was used as detector operating both in positive and negative ionization mode and operated by multiple-reaction monitoring (MRM) scanning. Noncompartmental pharmacokinetic parameters were calculated by DAS 2.0 program. The differences between each group were compared by SPSS 16.0 with Independent-Samples T -test. The pharmacokinetic parameters (such as C max , T max , T 1/2 , AUC 0– T , MRT 0– T , V z / F or CL z / F ) of all the detected components between the single herb (RAS or RPA) and herb pair (RRHP) showed significant differences ( P
-
uplc q tof ms coupled with multivariate statistical analysis as a powerful technique for rapidly exploring potential chemical markers to differentiate between Radix Paeoniae Alba and Radix Paeoniae rubra
Natural Product Communications, 2013Co-Authors: Wen Ding, Jing Wu, Dawei Qian, Zhenhao Li, Yefei Qian, Jinao DuanAbstract:Abstract To explore rapidly the potential chemical markers for differentiating Radix Paeoniae Alba and Radix Paeoniae Rubra, a method is proposed based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) coupled with multivariate statistical analysis. Batches of commercial samples were analyzed by UPLC-Q-TOF/MS. The datasets of t(R)-m/z pair, ion intensities and sample codes were further processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to compare holistically the difference between these two kinds of samples. Then statistics were used to generate an S-plot, in which the variables (t(R)-m/z pair) contributing most to the difference were clearly depicted as points at the two ends of "S", and the components correlated to these ions should be regarded as the chemical markers. The identities of the most changed markers can be identified by comparing the mass/UV spectra and retention times with those of reference compounds and/or tentatively assigned by matching empirical molecular formulae with those of known compounds published in the literature. Using this proposed approach, albflorin, paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, galloylalbiflorin and paeoniflorigenone were found to be the differentiating components for discrimination of Radix Paeoniae Alba and Radix Paeoniae Rubra. Moreover, paeoniflorin sulfonate and its isomer, isomaltopaeoniflorin sulfonate, were found to be the characteristic markers for all Radix Paeoniae Alba samples that were processed by sulfurdioxide gas fumigation. The results suggested that this newly established approach could be used to explore rapidly the potential chemical markers for herbs with similar chemical characteristics.
Zhiqing Liao - One of the best experts on this subject based on the ideXlab platform.
-
effect of aqueous extracts of scutellaria baicalensis georgi and Radix Paeoniae Alba on the serum igg1 and igg2a of the periodontitis mice
Chinese journal of stomatology, 2014Co-Authors: Ning Song, Fangli Lu, Shiguang Huang, Guicong Ding, Zhumin Zhou, Zhiqing LiaoAbstract:Objective To examine the effect of aqueous extracts of Scutellaria baicalensis Georgi and Radix Paeoniae Alba on periodontitis mice and compare the results of the two herbs for the treatment of the periodontitis mice.Methods Sixty-four SPF 12-week-old male Kunming mice were selected and randomly divided into four groups:Control group (C) ;Experimental periodontitis group (P):the peridontitis models in Kunming mice were prepared by wrapping silk ligature and inoculating with putative periodontopathic bacteria; Scutellaria baicalensis Georgi treatment group(SG):periodontitis was induced by the same method described above,the mice were gavaged with Scutellaria baicalensis Georgi; Radix Paeoniae Alba treatment group (RG):periodontitis was induced by the same method described above,the mice were gavaged with Radix Paeoniae Alba.Four mice were sacrificed at each time point of the end of 4,6,8 and 10 weeks in each group.The histopathological changes of periodontal tissue were observed under microscope with HE staining.The level of serum IgG1 and IgG2a was measured by enzyme-linked immunosorbent assay(ELISA).Results A serious inflammatory response,alveolar progressive absorption and a large number of osteoclasts were observed in the experimental periodontitis group.However,in SG and RG,the inflammation of the periodontal tissue was decreased and tissue repair was significant.The level of serum IgG2a in SG(6 week:0.934 ± 0.006,8 week:0.743 ± 0.009,10 week:0.674 ± 0.008) and RG (6 week:1.023 ± 0.032,8 week:0.851 ± 0.032,10 week:0.790 ± 0.009) was significantly decreased after the mice were gavaged with the two herbs(P ＜ 0.01).The level of serum IgG2a in SG was significantly lower than that of RG(P ＜0.01).The level of serum IgG1 in SG(6 week:0.314 ±0.006,8 week:0.344 ± 0.004,10 week:0.367 ±0.006) and RG(6 week:0.287 ±0.005,8 week:0.303 ±0.058,10 week:0.336 ±0.006) were significantly increased(P ＜ 0.01).The level of serum IgG1 in SG was significantly higher than that of RG (P ＜ 0.0l).Conclusions Both the aqueous extracts of Scutellaria baicalensis Georgi and Radix Paeoniae Alba showed therapeutic effect on periodontitis in mice.Scutellaria baicalensis Georgi was more effective than Radix Paeoniae Alba. Key words: Periodontitis; Scutellaria baicalensis; Thl-Th2 balance; Radix Paeoniae Alba
-
Effect of aqueous extracts of Scutellaria baicalensis Georgi and Radix Paeoniae Alba on the serum IgG1 and IgG2a of the periodontitis mice
Chinese journal of stomatology, 2014Co-Authors: Ning Song, Fangli Lu, Shiguang Huang, Guicong Ding, Zhumin Zhou, Zhiqing LiaoAbstract:OBJECTIVE: To examine the effect of aqueous extracts of Scutellaria baicalensis Georgi and Radix Paeoniae Alba on periodontitis mice and compare the results of the two herbs for the treatment of the periodontitis mice. METHODS: Sixty-four SPF 12-week-old male Kunming mice were selected and randomly divided into four groups:Control group(C); Experimental periodontitis group (P):the peridontitis models in Kunming mice were prepared by wrapping silk ligature and inoculating with putative periodontopathic bacteria; Scutellaria baicalensis Georgi treatment group (SG): periodontitis was induced by the same method described above, the mice were gavaged with Scutellaria baicalensis Georgi; Radix Paeoniae Alba treatment group (RG): periodontitis was induced by the same method described above, the mice were gavaged with Radix Paeoniae Alba.Four mice were sacrificed at each time point of the end of 4, 6, 8 and 10 weeks in each group. The histopathological changes of periodontal tissue were observed under microscope with HE staining. The level of serum IgG1 and IgG2a was measured by enzyme-linked immunosorbent assay (ELISA) . RESULTS: A serious inflammatory response, alveolar progressive absorption and a large number of osteoclasts were observed in the experimental periodontitis group.However, in SG and RG, the inflammation of the periodontal tissue was decreased and tissue repair was significant. The level of serum IgG2a in SG (6 week:0.934 ± 0.006, 8 week:0.743 ± 0.009, 10 week: 0.674 ± 0.008) and RG (6 week: 1.023 ± 0.032, 8 week: 0.851 ± 0.032, 10 week:0.790 ± 0.009) was significantly decreased after the mice were gavaged with the two herbs(P < 0.01). The level of serum IgG2a in SG was significantly lower than that of RG (P < 0.01). The level of serum IgG1 in SG (6 week: 0.314 ± 0.006, 8 week: 0.344 ± 0.004, 10 week: 0.367 ± 0.006) and RG (6 week: 0.287 ± 0.005, 8 week: 0.303 ± 0.058, 10 week: 0.336 ± 0.006) were significantly increased (P < 0.01). The level of serum IgG1 in SG was significantly higher than that of RG (P < 0.01). CONCLUSIONS: Both the aqueous extracts of Scutellaria baicalensis Georgi and Radix Paeoniae Alba showed therapeutic effect on periodontitis in mice.Scutellaria baicalensis Georgi was more effective than Radix Paeoniae Alba.
