The Experts below are selected from a list of 1278 Experts worldwide ranked by ideXlab platform
Yi-ying Wu - One of the best experts on this subject based on the ideXlab platform.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Background Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. Methods The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. Results RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. Conclusions This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the nf κb and mitogen activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
Yizeng Lian - One of the best experts on this subject based on the ideXlab platform.
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analysis of volatile oil in rhizoma ligustici chuanxiong Radix Paeoniae Rubra by gas chromatography mass spectrometry and chemometric resolution
Acta Pharmacologica Sinica, 2006Co-Authors: Xiaoru Li, Yizeng LianAbstract:Aim: To analyze the volatile chemical components of the herbal pair Rhizoma Ligustici Chuanxiong-Radix Paeoniae Rubra (RLC-RPR) and compare them with those of each of the herbs alone. Methods: Gas chromatography-mass spectrometry (GC-MS), a chemometric resolution technique using the heuristic evolving latent projections (HELP) method, and the overall volume integration method were used. Results: In total, 52, 38, and 61 volatile chemical components in RLC, RPR, and RLC-RPR essential oils were determined, respectively, accounting for 95.14%, 95.19%, and 89.68% of the total contents of essential oil of RLC, RPR, and RLC-RPR, respectively. The main volatile chemical components were butyldienephthalide (20.65%) and ligustilide (50.15%) for RLC; and n -hexadecanoic acid (20.18%), [Z,Z]9,12-octadecadienoic acid (30.11%), 2-hydroxy-benzaldehyde (17.08%) for RPR, and butyldienephthalide (14.80%), and ligustilide (38.91%) for RLC-RPR. The main volatile chemical components of RLC-RPR were almost the same as those of RLC, but the relative amounts were altered. Conclusion: The number of volatile chemical components in RLC-RPR was almost equal to the sum of the number in the 2 constituent herbs, but the relative amounts were altered. Furthermore, an acid-base reaction takes place during the process of decocting the herbs. The data gathered in this study may be helpful for understanding the synergistic nature of this herb pair in traditional Chinese medicine.
Huey-en Tzeng - One of the best experts on this subject based on the ideXlab platform.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Background Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. Methods The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. Results RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. Conclusions This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the nf κb and mitogen activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
Xiaoru Li - One of the best experts on this subject based on the ideXlab platform.
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analysis of volatile oil in rhizoma ligustici chuanxiong Radix Paeoniae Rubra by gas chromatography mass spectrometry and chemometric resolution
Acta Pharmacologica Sinica, 2006Co-Authors: Xiaoru Li, Yizeng LianAbstract:Aim: To analyze the volatile chemical components of the herbal pair Rhizoma Ligustici Chuanxiong-Radix Paeoniae Rubra (RLC-RPR) and compare them with those of each of the herbs alone. Methods: Gas chromatography-mass spectrometry (GC-MS), a chemometric resolution technique using the heuristic evolving latent projections (HELP) method, and the overall volume integration method were used. Results: In total, 52, 38, and 61 volatile chemical components in RLC, RPR, and RLC-RPR essential oils were determined, respectively, accounting for 95.14%, 95.19%, and 89.68% of the total contents of essential oil of RLC, RPR, and RLC-RPR, respectively. The main volatile chemical components were butyldienephthalide (20.65%) and ligustilide (50.15%) for RLC; and n -hexadecanoic acid (20.18%), [Z,Z]9,12-octadecadienoic acid (30.11%), 2-hydroxy-benzaldehyde (17.08%) for RPR, and butyldienephthalide (14.80%), and ligustilide (38.91%) for RLC-RPR. The main volatile chemical components of RLC-RPR were almost the same as those of RLC, but the relative amounts were altered. Conclusion: The number of volatile chemical components in RLC-RPR was almost equal to the sum of the number in the 2 constituent herbs, but the relative amounts were altered. Furthermore, an acid-base reaction takes place during the process of decocting the herbs. The data gathered in this study may be helpful for understanding the synergistic nature of this herb pair in traditional Chinese medicine.
Shen-chieh Chou - One of the best experts on this subject based on the ideXlab platform.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Background Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. Methods The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. Results RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. Conclusions This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
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Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the nf κb and mitogen activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.
