The Experts below are selected from a list of 3375 Experts worldwide ranked by ideXlab platform
Zhengtao Wang - One of the best experts on this subject based on the ideXlab platform.
-
metabolomics study of anti platelet effect of Radix Paeoniae rubra and Radix Paeoniae alba by uplc ms
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. METHOD: After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. CONCLUSION: Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
metabolomics study of anti inflammatory action of Radix Paeoniae rubra and Radix Paeoniae alba by ultraperformance liquid chromatography mass spectrometry
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Lan Lu, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. METHOD: To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. CONCLUSION: The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Yuqi He, Zhengtao Wang, Xuemei Cheng, Rui Wang, Cheng WuAbstract:Abstract An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69 ± 1.46 and 1.46 ± 0.29 (p 0.05) and a larger AUC (24.89 ± 7.41 μg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the tmax, Cmax, AUC, t1/2, CL, and Vd among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of tmax, Cmax, and AUC of paeoniflorin were remarkably increased (P
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Xuemei Cheng, Rui Wang, Jing CaoAbstract:An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69+/-1.46 and 1.46+/-0.29 (p<0.05)microg/ml at 1.67+/-0.43 and 0.80+/-0.35 h (p<0.05), respectively. Compared to the AUC (18.85+/-7.54 microg h/ml) after oral administration of the paeoniflorin solution, a smaller AUC (10.61+/-1.51 microg h/ml, p<0.05) and a larger AUC (24.89+/-7.41 microg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the t(max), C(max), AUC, t(1/2), CL, and V(d) among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of t(max), C(max), and AUC of paeoniflorin were remarkably increased (P<0.05, P<0.001) when oral administering paeoniflorin in the decoctions of Radix Paeoniae Rubra, but t(1/2), V(d), and CL were decreased (P<0.05 or P<0.01), in comparison of the decoction of Radix Paeoniae Alba.
-
Comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae Rubra and Radix Paeoniae Alba in rats
Journal of ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Xuemei Cheng, Rui Wang, Jing CaoAbstract:An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69+/-1.46 and 1.46+/-0.29 (p
Rui Wang - One of the best experts on this subject based on the ideXlab platform.
-
Radix Paeoniae rubra and Radix Paeoniae alba attenuate ccl4 induced acute liver injury an ultra performance liquid chromatography mass spectrometry uplc ms based metabolomic approach for the pharmacodynamic study of traditional chinese medicines tcms
International Journal of Molecular Sciences, 2012Co-Authors: Rui Wang, Aizhen Xiong, Zhongqiu Teng, Qiwei Yang, Lili YangAbstract:Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl4-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl4 injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
-
metabolomics study of anti platelet effect of Radix Paeoniae rubra and Radix Paeoniae alba by uplc ms
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. METHOD: After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. CONCLUSION: Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
metabolomics study of anti inflammatory action of Radix Paeoniae rubra and Radix Paeoniae alba by ultraperformance liquid chromatography mass spectrometry
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Lan Lu, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. METHOD: To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. CONCLUSION: The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Yuqi He, Zhengtao Wang, Xuemei Cheng, Rui Wang, Cheng WuAbstract:Abstract An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69 ± 1.46 and 1.46 ± 0.29 (p 0.05) and a larger AUC (24.89 ± 7.41 μg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the tmax, Cmax, AUC, t1/2, CL, and Vd among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of tmax, Cmax, and AUC of paeoniflorin were remarkably increased (P
-
comparative pharmacokinetic study of paeoniflorin after oral administration of decoction of Radix Paeoniae rubra and Radix Paeoniae alba in rats
Journal of Ethnopharmacology, 2008Co-Authors: Changhong Wang, Zhengtao Wang, Xuemei Cheng, Rui Wang, Jing CaoAbstract:An investigation was designed and conducted to compare the pharmacokinetics difference of paeoniflorin after oral administration of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba to rats on separate occasions. Quantification of paeoniflorin in rat plasma was achieved using a simple and rapid HPLC method for pharmacokinetic study. After oral administration of decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba, paeoniflorin was absorbed and reached a maximum concentration of 3.69+/-1.46 and 1.46+/-0.29 (p<0.05)microg/ml at 1.67+/-0.43 and 0.80+/-0.35 h (p<0.05), respectively. Compared to the AUC (18.85+/-7.54 microg h/ml) after oral administration of the paeoniflorin solution, a smaller AUC (10.61+/-1.51 microg h/ml, p<0.05) and a larger AUC (24.89+/-7.41 microg h/ml) of paeoniflorin after oral administration of the decoctions of Radix Paeoniae Alba and Radix Paeoniae Rubra were obtained, respectively. There were statistically significant differences in pharmacokinetic parameters of paeoniflorin including the t(max), C(max), AUC, t(1/2), CL, and V(d) among the animals orally administered the decoctions of Radix Paeoniae Rubra and Radix Paeoniae Alba. In particular, the parameters of t(max), C(max), and AUC of paeoniflorin were remarkably increased (P<0.05, P<0.001) when oral administering paeoniflorin in the decoctions of Radix Paeoniae Rubra, but t(1/2), V(d), and CL were decreased (P<0.05 or P<0.01), in comparison of the decoction of Radix Paeoniae Alba.
Jinao Duan - One of the best experts on this subject based on the ideXlab platform.
-
uplc q tof ms coupled with multivariate statistical analysis as a powerful technique for rapidly exploring potential chemical markers to differentiate between Radix Paeoniae alba and Radix Paeoniae rubra
Natural Product Communications, 2013Co-Authors: Wen Ding, Yefei Qian, Zhenhao Li, Jing Wu, Dawei Qian, Jinao DuanAbstract:Abstract To explore rapidly the potential chemical markers for differentiating Radix Paeoniae Alba and Radix Paeoniae Rubra, a method is proposed based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) coupled with multivariate statistical analysis. Batches of commercial samples were analyzed by UPLC-Q-TOF/MS. The datasets of t(R)-m/z pair, ion intensities and sample codes were further processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to compare holistically the difference between these two kinds of samples. Then statistics were used to generate an S-plot, in which the variables (t(R)-m/z pair) contributing most to the difference were clearly depicted as points at the two ends of "S", and the components correlated to these ions should be regarded as the chemical markers. The identities of the most changed markers can be identified by comparing the mass/UV spectra and retention times with those of reference compounds and/or tentatively assigned by matching empirical molecular formulae with those of known compounds published in the literature. Using this proposed approach, albflorin, paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, galloylalbiflorin and paeoniflorigenone were found to be the differentiating components for discrimination of Radix Paeoniae Alba and Radix Paeoniae Rubra. Moreover, paeoniflorin sulfonate and its isomer, isomaltopaeoniflorin sulfonate, were found to be the characteristic markers for all Radix Paeoniae Alba samples that were processed by sulfurdioxide gas fumigation. The results suggested that this newly established approach could be used to explore rapidly the potential chemical markers for herbs with similar chemical characteristics.
-
UPLC-Q-TOF/MS coupled with multivariate statistical analysis as a powerful technique for rapidly exploring potential chemical markers to differentiate between Radix Paeoniae alba and Radix Paeoniae rubra.
Natural Product Communications, 2013Co-Authors: Wen Ding, Yefei Qian, Zhenhao Li, Jing Wu, Dawei Qian, Jinao DuanAbstract:Abstract To explore rapidly the potential chemical markers for differentiating Radix Paeoniae Alba and Radix Paeoniae Rubra, a method is proposed based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) coupled with multivariate statistical analysis. Batches of commercial samples were analyzed by UPLC-Q-TOF/MS. The datasets of t(R)-m/z pair, ion intensities and sample codes were further processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to compare holistically the difference between these two kinds of samples. Then statistics were used to generate an S-plot, in which the variables (t(R)-m/z pair) contributing most to the difference were clearly depicted as points at the two ends of "S", and the components correlated to these ions should be regarded as the chemical markers. The identities of the most changed markers can be identified by comparing the mass/UV spectra and retention times with those of reference compounds and/or tentatively assigned by matching empirical molecular formulae with those of known compounds published in the literature. Using this proposed approach, albflorin, paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, galloylalbiflorin and paeoniflorigenone were found to be the differentiating components for discrimination of Radix Paeoniae Alba and Radix Paeoniae Rubra. Moreover, paeoniflorin sulfonate and its isomer, isomaltopaeoniflorin sulfonate, were found to be the characteristic markers for all Radix Paeoniae Alba samples that were processed by sulfurdioxide gas fumigation. The results suggested that this newly established approach could be used to explore rapidly the potential chemical markers for herbs with similar chemical characteristics.
S U Xianzhong - One of the best experts on this subject based on the ideXlab platform.
-
study on the extraction and purification of the antimicrobial active constituents from Radix Paeoniae rubra by using hp20 resin
Journal of Anhui Agricultural Sciences, 2011Co-Authors: S U XianzhongAbstract:[Objective] To extract and determine the major antimicrobial active constituents from Radix Paeoniae RUBRA.[Method] The active constituents of Radix Paeoniae RUBRA was firstly prepared with 90% ethanol,refined with other chemicals and then purified with HP20 resin,and the in vitro antimicrobial effect of the two-fold dilution of extracts was experimented against Klebsiella pneumoniae ATCC 49790,Acinetobacter baumannii 36 and Serratia marcescens ATCC 14041.The FTIR spectra of the extracts were analyzed.[Result] The antimicrobial activity of the 100% elution extract of Radix Paeoniae RUBRA was the highest and the MICs were 32.00,32.00 and 125.00 g/L respectively;the FTIR spectra of the extracts was very similar to that of peoniflorin.[Conclusion] Peoniflorin was the main active constituents of Radix Paeoniae RUBRA,it had significant antimicrobial effect.
-
extraction and purification of the antimicrobial active components from Radix Paeoniae rubra by using hp20 resin
Medicinal Plant, 2011Co-Authors: S U Xianzhong, Zheng Quanxi, Li Ming, Liu ShoukunAbstract:[Objective] The aim was to extract and determine the major antimicrobial active constituents from Radix Paeoniae RUBRA. [Method] Radix Paeoniae RUBRA was treated by ethanol ultrasonic extraction, HP20 colophony column chromatogram and other processes. And the in vitro antimicrobial tests on Klebsiella pneumoniae ATCC 49790, Acinetobacter baumannii 36 and Serratia marcescens ATCC 14041 were carried out by the two-fold dilution of extracts. FTIR spectra of the extracts were analyzed by Fourier transform infrared spectroscopy. [Result] 100% ethanol elution had the optimum inhibitory effects on the three strains, with the MICs of 32.00, 32.00 and 125.00 g/L, respectively. The FTIR spectrum of the extracts was very similar to that of peoniflorin. [Conclusion] Peoniflorin was the main active components of Radix Paeoniae RUBRA, and had significant antimicrobial effect.
Qiwei Yang - One of the best experts on this subject based on the ideXlab platform.
-
Radix Paeoniae rubra and Radix Paeoniae alba attenuate ccl4 induced acute liver injury an ultra performance liquid chromatography mass spectrometry uplc ms based metabolomic approach for the pharmacodynamic study of traditional chinese medicines tcms
International Journal of Molecular Sciences, 2012Co-Authors: Rui Wang, Aizhen Xiong, Zhongqiu Teng, Qiwei Yang, Lili YangAbstract:Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl4-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl4 injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
-
metabolomics study of anti platelet effect of Radix Paeoniae rubra and Radix Paeoniae alba by uplc ms
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. METHOD: After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. CONCLUSION: Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
-
metabolomics study of anti inflammatory action of Radix Paeoniae rubra and Radix Paeoniae alba by ultraperformance liquid chromatography mass spectrometry
China Journal of Chinese Matera Medica, 2011Co-Authors: Qiwei Yang, Aizhen Xiong, Lan Lu, Rui Wang, Zhengtao WangAbstract:OBJECTIVE: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis. METHOD: To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA. RESULT: Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs. CONCLUSION: The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.