Ragweed Pollen

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Philippe Moingeon - One of the best experts on this subject based on the ideXlab platform.

  • New Insights into Ragweed Pollen Allergens
    Current Allergy and Asthma Reports, 2015
    Co-Authors: Véronique Bordas-le Floch, Rachel Groeme, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Henri Chabre, Philippe Moingeon
    Abstract:

    Pollen allergens from short Ragweed ( Ambrosia artemisiifolia ) cause severe respiratory allergies in North America and Europe. To date, ten short Ragweed Pollen allergens belonging to eight protein families, including the recently discovered novel major allergen Amb a 11, have been recorded in the International Union of Immunological Societies (IUIS) allergen database. With evidence that other components may further contribute to short Ragweed Pollen allergenicity, a better understanding of the allergen repertoire is a requisite for the design of proper diagnostic tools and efficient immunotherapies. This review provides an update on both known as well as novel candidate allergens from short Ragweed Pollen, identified through a comprehensive characterization of the Ragweed Pollen transcriptome and proteome.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
    PloS one, 2015
    Co-Authors: Véronique Bordas-le Floch, Julien Bouley, Rachel Groeme, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Maxime Le Mignon, K. Jain, Philippe Moingeon
    Abstract:

    Background Allergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.

  • 2D-gel reference map of the short Ragweed Pollen proteome.
    2015
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    Proteins from an aqueous short Ragweed Pollen extract were separated by 2D-gel electrophoresis and stained with Sypro Ruby. Proteins spots were picked and analyzed by LC-MS/MS after trypsin digestion. Proteins were identified using the Transcriptome-Derived Proteome collection supplemented with missing known allergens. Numbers refer to spots analyzed by mass spectrometry. Identification details are provided in Table 1.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches
    2015
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    BackgroundAllergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.MethodsFollowing a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short Ragweed Pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.ResultsShort Ragweed Pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short Ragweed Pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short Ragweed Pollen extract resolved over 2D-gels. Combined with information derived from the annotated Pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20–70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.ConclusionsWe extended the list of allergens identified in short Ragweed Pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
    Public Library of Science (PLoS), 1
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    Allergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.Following a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short Ragweed Pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.Short Ragweed Pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short Ragweed Pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short Ragweed Pollen extract resolved over 2D-gels. Combined with information derived from the annotated Pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20-70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.We extended the list of allergens identified in short Ragweed Pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes

Matt Smith - One of the best experts on this subject based on the ideXlab platform.

  • mesoscale atmospheric transport of Ragweed Pollen allergens from infected to uninfected areas
    International Journal of Biometeorology, 2016
    Co-Authors: łukasz Grewling, Branko Šikoparija, Matt Smith, Carsten Ambelas Skjøth, Pawel Bogawski, Dorota Jenerowicz, Magdalena Czarneckaoperacz
    Abstract:

    Allergenic Ragweed (Ambrosia spp.) Pollen grains, after being released from anthers, can be dispersed by air masses far from their source. However, the action of air temperature, humidity and solar radiation on Pollen grains in the atmosphere could impact on the ability of long distance transported (LDT) Pollen to maintain allergenic potency. Here, we report that the major allergen of Ambrosia artemisiifolia Pollen (Amb a 1) collected in ambient air during episodes of LDT still have immunoreactive properties. The amount of Amb a 1 found in LDT Ragweed Pollen grains was not constant and varied between episodes. In addition to allergens in Pollen sized particles, we detected reactive Amb a 1 in subPollen sized respirable particles. These findings suggest that Ragweed Pollen grains have the potential to cause allergic reactions, not only in the heavily infested areas but, due to LDT episodes, also in the regions unaffected by Ragweed populations.

  • Modelling Atmospheric Concentrations of Ragweed Pollen From Local and Distant Sources
    2014
    Co-Authors: Branko Šikoparija, Michel Thibaudon, J. Sommer, Matt Smith, Łukasz Grewling, Carsten Ambelas Skjøth
    Abstract:

    Pollen grains from the genus Ragweed (Ambrosia spp.) are important aeroallergens. In Europe, the largest sources of atmospheric Ragweed Pollen are the Rhone Valley (France), parts of Northern Italy, the Pannonian Plain and Ukraine. Episodes of Long Distance Transport (LDT) of Ragweed Pollen from these centres can cover large parts of Europe and are predominantly studied using receptor based models (Smith et al., (2013) and references therein). The clinical impact of allergenic Ragweed Pollen arriving from distant sources remains unclear (Cecchi et al. 2010). Although a recent study has found the major allergens of Ragweed in air samples collected in Poznan, Poland, during episodes of long-distance transport from the Pannonian Plain (Grewling et al. 2013). The source orientated models SILAM, DEHM, COSMO-Art, METRAS and ENVIRO-HIRLAM currently report having the capability of modelling atmospheric concentrations of Pollen in Europe. The performance of such source-orientated models is strongly dependent on the quality of the emissions data, which is a focus of current research (e.g. Thibaudon et al. (2014)). The output from these models are important for warning allergy sufferers in areas polluted by Ragweed, but could also be used to warn the public of Ragweed Pollen being transported into areas where the plant is not abundant. Areas outside of the main areas of Ragweed infection that contain considerable local populations must, however, also include local scale models. These models can be used to predict local concentrations, even when LDT is not present. This concept of combined LDT and local scale calculations has been shown to be work for air pollutants and is considered usable for urban scale calculations of aeroallergens once urban scale maps of aeroallergen sources have been produced.

  • Ragweed Pollen source inventory for france the second largest centre of ambrosia in europe
    Atmospheric Environment, 2014
    Co-Authors: Michel Thibaudon, Branko Šikoparija, Matt Smith, Gilles Oliver, Carsten Ambelas Skjøth
    Abstract:

    France, in particular the Rhone-Alpes region, is one of the three main centres of Ragweed (Ambrosia) in Europe. The aim of this study is to develop a gridded Ragweed Pollen source inventory for all of France that can be used in assessments, eradication plans and by atmospheric models for describing concentrations of airborne Ragweed Pollen. The inventory combines information about spatial variations in annual Ambrosia Pollen counts, knowledge of Ragweed ecology, detailed land cover information and a Digital Elevation Model. The Ragweed inventory consists of a local infection level on a scale of 0–100% (where 100% is the highest plant abundance per area in the studied region) and a European infection level between 0% and 100% (where 100% relates to the highest identified plant abundance in Europe using the same methodology) that has been distributed onto the EMEP grid with 5 km × 5 km resolution. The results of this analysis showed that some of the highest mean annual Ragweed Pollen concentrations were recorded at Roussillon in the Rhone-Valley. This is reflected by the inventory, where the European infection level has been estimated to reach 67.70% of the most infected areas in Europe i.e. Kecskemet in central Hungary. The inventory shows that the Rhone Valley is the most heavily infected part of France. Central France is also infected, but northern and western parts of France are much less infected. The inventory can be entered into atmospheric transport models, in combination with other components such as a phenological model and a model for daily Pollen release, in order to simulate the dispersion of Ragweed Pollen within France as well as potential long-distance transport from France to other European countries.

  • Ragweed Pollen source inventory for France – The second largest centre of Ambrosia in Europe
    Atmospheric Environment, 2014
    Co-Authors: Michel Thibaudon, Branko Šikoparija, Matt Smith, Gilles Oliver, Carsten Ambelas Skjøth
    Abstract:

    France, in particular the Rhone-Alpes region, is one of the three main centres of Ragweed (Ambrosia) in Europe. The aim of this study is to develop a gridded Ragweed Pollen source inventory for all of France that can be used in assessments, eradication plans and by atmospheric models for describing concentrations of airborne Ragweed Pollen. The inventory combines information about spatial variations in annual Ambrosia Pollen counts, knowledge of Ragweed ecology, detailed land cover information and a Digital Elevation Model. The Ragweed inventory consists of a local infection level on a scale of 0–100% (where 100% is the highest plant abundance per area in the studied region) and a European infection level between 0% and 100% (where 100% relates to the highest identified plant abundance in Europe using the same methodology) that has been distributed onto the EMEP grid with 5 km × 5 km resolution. The results of this analysis showed that some of the highest mean annual Ragweed Pollen concentrations were recorded at Roussillon in the Rhone-Valley. This is reflected by the inventory, where the European infection level has been estimated to reach 67.70% of the most infected areas in Europe i.e. Kecskemet in central Hungary. The inventory shows that the Rhone Valley is the most heavily infected part of France. Central France is also infected, but northern and western parts of France are much less infected. The inventory can be entered into atmospheric transport models, in combination with other components such as a phenological model and a model for daily Pollen release, in order to simulate the dispersion of Ragweed Pollen within France as well as potential long-distance transport from France to other European countries.

Carsten Ambelas Skjøth - One of the best experts on this subject based on the ideXlab platform.

  • mesoscale atmospheric transport of Ragweed Pollen allergens from infected to uninfected areas
    International Journal of Biometeorology, 2016
    Co-Authors: łukasz Grewling, Branko Šikoparija, Matt Smith, Carsten Ambelas Skjøth, Pawel Bogawski, Dorota Jenerowicz, Magdalena Czarneckaoperacz
    Abstract:

    Allergenic Ragweed (Ambrosia spp.) Pollen grains, after being released from anthers, can be dispersed by air masses far from their source. However, the action of air temperature, humidity and solar radiation on Pollen grains in the atmosphere could impact on the ability of long distance transported (LDT) Pollen to maintain allergenic potency. Here, we report that the major allergen of Ambrosia artemisiifolia Pollen (Amb a 1) collected in ambient air during episodes of LDT still have immunoreactive properties. The amount of Amb a 1 found in LDT Ragweed Pollen grains was not constant and varied between episodes. In addition to allergens in Pollen sized particles, we detected reactive Amb a 1 in subPollen sized respirable particles. These findings suggest that Ragweed Pollen grains have the potential to cause allergic reactions, not only in the heavily infested areas but, due to LDT episodes, also in the regions unaffected by Ragweed populations.

  • Modelling Atmospheric Concentrations of Ragweed Pollen From Local and Distant Sources
    2014
    Co-Authors: Branko Šikoparija, Michel Thibaudon, J. Sommer, Matt Smith, Łukasz Grewling, Carsten Ambelas Skjøth
    Abstract:

    Pollen grains from the genus Ragweed (Ambrosia spp.) are important aeroallergens. In Europe, the largest sources of atmospheric Ragweed Pollen are the Rhone Valley (France), parts of Northern Italy, the Pannonian Plain and Ukraine. Episodes of Long Distance Transport (LDT) of Ragweed Pollen from these centres can cover large parts of Europe and are predominantly studied using receptor based models (Smith et al., (2013) and references therein). The clinical impact of allergenic Ragweed Pollen arriving from distant sources remains unclear (Cecchi et al. 2010). Although a recent study has found the major allergens of Ragweed in air samples collected in Poznan, Poland, during episodes of long-distance transport from the Pannonian Plain (Grewling et al. 2013). The source orientated models SILAM, DEHM, COSMO-Art, METRAS and ENVIRO-HIRLAM currently report having the capability of modelling atmospheric concentrations of Pollen in Europe. The performance of such source-orientated models is strongly dependent on the quality of the emissions data, which is a focus of current research (e.g. Thibaudon et al. (2014)). The output from these models are important for warning allergy sufferers in areas polluted by Ragweed, but could also be used to warn the public of Ragweed Pollen being transported into areas where the plant is not abundant. Areas outside of the main areas of Ragweed infection that contain considerable local populations must, however, also include local scale models. These models can be used to predict local concentrations, even when LDT is not present. This concept of combined LDT and local scale calculations has been shown to be work for air pollutants and is considered usable for urban scale calculations of aeroallergens once urban scale maps of aeroallergen sources have been produced.

  • Ragweed Pollen source inventory for france the second largest centre of ambrosia in europe
    Atmospheric Environment, 2014
    Co-Authors: Michel Thibaudon, Branko Šikoparija, Matt Smith, Gilles Oliver, Carsten Ambelas Skjøth
    Abstract:

    France, in particular the Rhone-Alpes region, is one of the three main centres of Ragweed (Ambrosia) in Europe. The aim of this study is to develop a gridded Ragweed Pollen source inventory for all of France that can be used in assessments, eradication plans and by atmospheric models for describing concentrations of airborne Ragweed Pollen. The inventory combines information about spatial variations in annual Ambrosia Pollen counts, knowledge of Ragweed ecology, detailed land cover information and a Digital Elevation Model. The Ragweed inventory consists of a local infection level on a scale of 0–100% (where 100% is the highest plant abundance per area in the studied region) and a European infection level between 0% and 100% (where 100% relates to the highest identified plant abundance in Europe using the same methodology) that has been distributed onto the EMEP grid with 5 km × 5 km resolution. The results of this analysis showed that some of the highest mean annual Ragweed Pollen concentrations were recorded at Roussillon in the Rhone-Valley. This is reflected by the inventory, where the European infection level has been estimated to reach 67.70% of the most infected areas in Europe i.e. Kecskemet in central Hungary. The inventory shows that the Rhone Valley is the most heavily infected part of France. Central France is also infected, but northern and western parts of France are much less infected. The inventory can be entered into atmospheric transport models, in combination with other components such as a phenological model and a model for daily Pollen release, in order to simulate the dispersion of Ragweed Pollen within France as well as potential long-distance transport from France to other European countries.

  • Ragweed Pollen source inventory for France – The second largest centre of Ambrosia in Europe
    Atmospheric Environment, 2014
    Co-Authors: Michel Thibaudon, Branko Šikoparija, Matt Smith, Gilles Oliver, Carsten Ambelas Skjøth
    Abstract:

    France, in particular the Rhone-Alpes region, is one of the three main centres of Ragweed (Ambrosia) in Europe. The aim of this study is to develop a gridded Ragweed Pollen source inventory for all of France that can be used in assessments, eradication plans and by atmospheric models for describing concentrations of airborne Ragweed Pollen. The inventory combines information about spatial variations in annual Ambrosia Pollen counts, knowledge of Ragweed ecology, detailed land cover information and a Digital Elevation Model. The Ragweed inventory consists of a local infection level on a scale of 0–100% (where 100% is the highest plant abundance per area in the studied region) and a European infection level between 0% and 100% (where 100% relates to the highest identified plant abundance in Europe using the same methodology) that has been distributed onto the EMEP grid with 5 km × 5 km resolution. The results of this analysis showed that some of the highest mean annual Ragweed Pollen concentrations were recorded at Roussillon in the Rhone-Valley. This is reflected by the inventory, where the European infection level has been estimated to reach 67.70% of the most infected areas in Europe i.e. Kecskemet in central Hungary. The inventory shows that the Rhone Valley is the most heavily infected part of France. Central France is also infected, but northern and western parts of France are much less infected. The inventory can be entered into atmospheric transport models, in combination with other components such as a phenological model and a model for daily Pollen release, in order to simulate the dispersion of Ragweed Pollen within France as well as potential long-distance transport from France to other European countries.

Rudolf Valenta - One of the best experts on this subject based on the ideXlab platform.

  • Ragweed Pollen allergy burden characteristics and management of an imported allergen source in europe
    International Archives of Allergy and Immunology, 2018
    Co-Authors: Kuanwei Chen, Rudolf Valenta, Laura Marusciac, Paul Tudor Tamas, Carmen Panaitescu
    Abstract:

    Ambrosia artemisiifolia, also known as common or short Ragweed, is an invasive annual flowering herbaceous plant that has its origin in North America. Nowadays, Ragweed can be found in many areas worldwide. Ragweed Pollen is known for its high potential to cause type I allergic reactions in late summer and autumn and represents a major health problem in America and several countries in Europe. Climate change and urbanization, as well as long distance transport capacity, enhance the spread of Ragweed Pollen. Therefore Ragweed is becoming domestic in non-invaded areas which in turn will increase the sensitization rate. So far 11 Ragweed allergens have been described and, according to IgE reactivity, Amb a 1 and Amb a 11 seem to be major allergens. Sensitization rates of the other allergens vary between 10 and 50%. Most of the allergens have already been recombinantly produced, but most of them have not been characterized regarding their allergenic activity, therefore no conclusion on the clinical relevance of all the allergens can be made, which is important and necessary for an accurate diagnosis. Pharmacotherapy is the most common treatment for Ragweed Pollen allergy but fails to impact on the course of allergy. Allergen-specific immunotherapy (AIT) is the only causative and disease-modifying treatment of allergy with long-lasting effects, but currently it is based on the administration of Ragweed Pollen extract or Amb a 1 only. In order to improve Ragweed Pollen AIT, new strategies are required with higher efficacy and safety.

  • Identification of common allergenic structures in mugwort and Ragweed Pollen.
    The Journal of allergy and clinical immunology, 1998
    Co-Authors: Reinhold Hirschwehr, Claudia Heppner, F. Horak, S. De Jager, D Kraft, Susanne Spitzauer, Peter Valent, U. Berger, Wolfgang R Sperr, Rudolf Valenta
    Abstract:

    Identification of common allergenic structures in mugwort and Ragweed Pollen. Despite the rare occurrence of Ragweed in Middle Europe, a surprisingly high number of patients allergic to mugwort, a frequently encountered weed, display IgE reactivity against Ragweed Pollen allergens. The aim of this study was to investigate whether the high prevalence of IgE reactivity against Ragweed in patients allergic to mugwort is caused by the presence of common allergenic determinants. We also sought to characterize any cross-reactive allergens. Common allergenic structures in mugwort and Ragweed Pollen were characterized by qualitative IgE immunoblot inhibition experiments performed with natural allergen extracts and recombinant allergens. The degree of cross-reactivity was estimated by quantitative CAP-FEIA competitions. The clinical significance of cross-reactive IgE antibodies was studied with histamine release experiments and nasal provocation tests. Mugwort and Ragweed RAST values were significantly correlated in a population of 82 Austrian patients allergic to mugwort. IgE antibodies cross-reacted with allergens of comparable molecular weight that were present in both extracts. By using recombinant birch profilin and specific antisera for IgE inhibition experiments, profilin was identified as one of the cross-reactive components in mugwort and Ragweed Pollen. Preincubation of sera from patients allergic to mugwort with mugwort extract inhibited IgE binding to Ragweed Pollen extract greater than 80%. Mugwort and Ragweed Pollen extract induced comparable histamine release and reduction of nasal air flow in a patient with IgE reactivity against the major mugwort allergen Art v 1. In addition to profilin, mugwort and Ragweed Pollen contain a number of cross-reactive allergens, among them the major mugwort allergen Art v 1. Cross-reactive IgE antibodies can lead to clinically significant allergic reactions.

  • identification of common allergenic structures in mugwort and Ragweed Pollen
    The Journal of Allergy and Clinical Immunology, 1998
    Co-Authors: Reinhold Hirschwehr, Claudia Heppner, F. Horak, S. De Jager, D Kraft, Susanne Spitzauer, Peter Valent, U. Berger, Wolfgang R Sperr, Rudolf Valenta
    Abstract:

    Abstract Background: Despite the rare occurrence of Ragweed in Middle Europe, a surprisingly high number of patients allergic to mugwort, a frequently encountered weed, display IgE reactivity against Ragweed Pollen allergens. Objective: The aim of this study was to investigate whether the high prevalence of IgE reactivity against Ragweed in patients allergic to mugwort is caused by the presence of common allergenic determinants. We also sought to characterize any cross-reactive allergens. Methods: Common allergenic structures in mugwort and Ragweed Pollen were characterized by qualitative IgE immunoblot inhibition experiments performed with natural allergen extracts and recombinant allergens. The degree of cross-reactivity was estimated by quantitative CAP-FEIA competitions. The clinical significance of cross-reactive IgE antibodies was studied with histamine release experiments and nasal provocation tests. Results: Mugwort and Ragweed RAST values were significantly correlated in a population of 82 Austrian patients allergic to mugwort. IgE antibodies cross-reacted with allergens of comparable molecular weight that were present in both extracts. By using recombinant birch profilin and specific antisera for IgE inhibition experiments, profilin was identified as one of the cross-reactive components in mugwort and Ragweed Pollen. Preincubation of sera from patients allergic to mugwort with mugwort extract inhibited IgE binding to Ragweed Pollen extract greater than 80%. Mugwort and Ragweed Pollen extract induced comparable histamine release and reduction of nasal air flow in a patient with IgE reactivity against the major mugwort allergen Art v 1. Conclusion: In addition to profilin, mugwort and Ragweed Pollen contain a number of cross-reactive allergens, among them the major mugwort allergen Art v 1. Cross-reactive IgE antibodies can lead to clinically significant allergic reactions. (J Allergy Clin Immunol 1998;101:196-206.)

Rachel Groeme - One of the best experts on this subject based on the ideXlab platform.

  • New Insights into Ragweed Pollen Allergens
    Current Allergy and Asthma Reports, 2015
    Co-Authors: Véronique Bordas-le Floch, Rachel Groeme, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Henri Chabre, Philippe Moingeon
    Abstract:

    Pollen allergens from short Ragweed ( Ambrosia artemisiifolia ) cause severe respiratory allergies in North America and Europe. To date, ten short Ragweed Pollen allergens belonging to eight protein families, including the recently discovered novel major allergen Amb a 11, have been recorded in the International Union of Immunological Societies (IUIS) allergen database. With evidence that other components may further contribute to short Ragweed Pollen allergenicity, a better understanding of the allergen repertoire is a requisite for the design of proper diagnostic tools and efficient immunotherapies. This review provides an update on both known as well as novel candidate allergens from short Ragweed Pollen, identified through a comprehensive characterization of the Ragweed Pollen transcriptome and proteome.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
    PloS one, 2015
    Co-Authors: Véronique Bordas-le Floch, Julien Bouley, Rachel Groeme, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Maxime Le Mignon, K. Jain, Philippe Moingeon
    Abstract:

    Background Allergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.

  • 2D-gel reference map of the short Ragweed Pollen proteome.
    2015
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    Proteins from an aqueous short Ragweed Pollen extract were separated by 2D-gel electrophoresis and stained with Sypro Ruby. Proteins spots were picked and analyzed by LC-MS/MS after trypsin digestion. Proteins were identified using the Transcriptome-Derived Proteome collection supplemented with missing known allergens. Numbers refer to spots analyzed by mass spectrometry. Identification details are provided in Table 1.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches
    2015
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    BackgroundAllergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.MethodsFollowing a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short Ragweed Pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.ResultsShort Ragweed Pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short Ragweed Pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short Ragweed Pollen extract resolved over 2D-gels. Combined with information derived from the annotated Pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20–70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.ConclusionsWe extended the list of allergens identified in short Ragweed Pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes.

  • Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
    Public Library of Science (PLoS), 1
    Co-Authors: Véronique Bordas-le Floch, Maxime Le Mignon, Julien Bouley, Rachel Groeme, Karine Jain, Véronique Baron-bodo, Emmanuel Nony, Laurent Mascarell, Philippe Moingeon
    Abstract:

    Allergy to short Ragweed (Ambrosia artemisiifolia) Pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short Ragweed Pollen allergens are officially recorded, patterns of IgE reactivity observed in Ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short Ragweed Pollen.Following a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short Ragweed Pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.Short Ragweed Pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short Ragweed Pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short Ragweed Pollen extract resolved over 2D-gels. Combined with information derived from the annotated Pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20-70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.We extended the list of allergens identified in short Ragweed Pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes