The Experts below are selected from a list of 20136 Experts worldwide ranked by ideXlab platform
Mikiko Sodeoka - One of the best experts on this subject based on the ideXlab platform.
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the alkyne tag Raman Imaging of coronatine a plant pathogen virulence factor in commelina communis and its possible mode of action
Organic and Biomolecular Chemistry, 2018Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Kengo Hayashi, Yasuhiro Ishimaru, Yousuke Takaoka, Mikiko SodeokaAbstract:We previously reported that coronatine, a virulence factor of plant bacteria, facilitates bacterial infection through an ER (endoplasmic reticulum)-mediated, non-canonical mechanism in the model dicot plant, Arabidopsis thaliana. Here, we report that this same ER-mechanism is ubiquitous among dicots and monocots, and works by affecting the ethylene signaling pathway widely found in plants. The subcellular localization of coronatine by the alkyne-tag Raman Imaging (ATRI) approach provided a convincing clue.
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noncanonical function of a small molecular virulence factor coronatine against plant immunity an in vivo Raman Imaging approach
ACS central science, 2017Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Yasuhiro Ishimaru, Yousuke Takaoka, Takeshi Nakano, Shinya Tsukiji, Mikiko SodeokaAbstract:Coronatine (1), a small-molecular virulence factor produced by plant-pathogenic bacteria, promotes bacterial infection by inducing the opening of stomatal pores, the major route of bacterial entry into the plant, via the jasmonate-mediated COI1-JAZ signaling pathway. However, this pathway is also important for multiple plant functions, including defense against wounding by herbivorous insects. Thus, suppression of the COI1-JAZ signaling pathway to block bacterial infection would concomitantly impair plant defense against herbivorous wounding. Here, we report additional, COI1-JAZ-independent, action of 1 in Arabidopsis thaliana guard cells. First, we found that a stereoisomer of 1 regulates the movement of Arabidopsis guard cells without affecting COI1-JAZ signaling. Second, we found using alkyne-tagged Raman Imaging (ATRI) that 1 is localized to the endoplasmic reticulum (ER) of living guard cells of Arabidopsis. The use of arc6 mutant lacking chloroplast formation was pivotal to circumvent the issue of a...
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Noncanonical Function of a Small-Molecular Virulence Factor Coronatine against Plant Immunity: An In Vivo Raman Imaging Approach
2017Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Yasuhiro Ishimaru, Yousuke Takaoka, Takeshi Nakano, Shinya Tsukiji, Mikiko SodeokaAbstract:Coronatine (1), a small-molecular virulence factor produced by plant-pathogenic bacteria, promotes bacterial infection by inducing the opening of stomatal pores, the major route of bacterial entry into the plant, via the jasmonate-mediated COI1-JAZ signaling pathway. However, this pathway is also important for multiple plant functions, including defense against wounding by herbivorous insects. Thus, suppression of the COI1-JAZ signaling pathway to block bacterial infection would concomitantly impair plant defense against herbivorous wounding. Here, we report additional, COI1-JAZ-independent, action of 1 in Arabidopsis thaliana guard cells. First, we found that a stereoisomer of 1 regulates the movement of Arabidopsis guard cells without affecting COI1-JAZ signaling. Second, we found using alkyne-tagged Raman Imaging (ATRI) that 1 is localized to the endoplasmic reticulum (ER) of living guard cells of Arabidopsis. The use of arc6 mutant lacking chloroplast formation was pivotal to circumvent the issue of autofluorescence during ATRI. These findings indicate that 1 has an ER-related action on Arabidopsis stomata that bypasses the COI1-JAZ signaling module. It may be possible to suppress the action of 1 on stomata without impairing plant defense responses against herbivores
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alkyne tag Raman Imaging for visualization of mobile small molecules in live cells
Journal of the American Chemical Society, 2012Co-Authors: Hiroyuki Yamakoshi, Kosuke Dodo, Almar F Palonpon, Jun Ando, Katsumasa Fujita, Satoshi Kawata, Mikiko SodeokaAbstract:Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous molecule in live cells. Here, we show that alkyne-tag Raman Imaging (ATRI) is a promising approach for visualizing nonimmobilized small molecules in live cells. An examination of structure–Raman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogues in live cells. Cellular concentrations of diyne-tagged CoQ analogues could be semiquantitatively estimated. Finally, simultaneous Imaging of two small molecules, 5-ethynyl-2′-deoxyuridine (EdU) and a CoQ analogue, with distinct Raman tags was demonstrated.
Marcus T Cicerone - One of the best experts on this subject based on the ideXlab platform.
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spectroscopic coherent Raman Imaging of caenorhabditis elegans reveals lipid particle diversity
Nature Chemical Biology, 2020Co-Authors: Weiwen Chen, George A Lemieux, Charles H Camp, Tachau Chang, Kaveh Ashrafi, Marcus T CiceroneAbstract:Caenorhabditis elegans serves as a model for understanding adiposity and its connections to aging. Current methodologies do not distinguish between fats serving the energy needs of the parent, akin to mammalian adiposity, from those that are distributed to the progeny, making it difficult to accurately interpret the physiological implications of fat content changes induced by external perturbations. Using spectroscopic coherent Raman Imaging, we determine the protein content, chemical profiles and dynamics of lipid particles in live animals. We find fat particles in the adult intestine to be diverse, with most destined for the developing progeny. In contrast, the skin-like epidermis contains fats that are the least heterogeneous, the least dynamic and have high triglyceride content. These attributes are most consistent with stored somatic energy reservoirs. These results challenge the prevailing practice of assessing C. elegans adiposity by measurements that are dominated by the intestinal fat content.
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chemically sensitive bioImaging with coherent Raman scattering
Nature Photonics, 2015Co-Authors: Charles H Camp, Marcus T CiceroneAbstract:Coherent Raman Imaging techniques have evolved to become powerful tools for biomedical Imaging without the need for labelling.
Satoshi Kawata - One of the best experts on this subject based on the ideXlab platform.
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time lapse Raman Imaging of osteoblast differentiation
Scientific Reports, 2015Co-Authors: Aya Hashimoto, Katsumasa Fujita, Satoshi Kawata, Yoshinori Yamaguchi, Liangda Chiu, Chiaki Morimoto, Masahide Takedachi, Shinya Murakami, Eiichi TamiyaAbstract:Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine the temporal changes in mineralization-related biomolecules at mineralization spots, we performed time-lapse Raman Imaging of mouse osteoblasts at a subcellular resolution throughout the mineralization process. Raman Imaging enabled us to analyze the dynamics of the related biomolecules at mineralization spots throughout the entire process of mineralization. Here, we stimulated KUSA-A1 cells to differentiate into osteoblasts and conducted time-lapse Raman Imaging on them every 4 hours for 24 hours, beginning 5 days after the stimulation. The HA and cytochrome c Raman bands were used as markers for osteoblastic mineralization and apoptosis. From the Raman images successfully acquired throughout the mineralization process, we found that β-carotene acts as a biomarker that indicates the initiation of osteoblastic mineralization. A fluctuation of cytochrome c concentration, which indicates cell apoptosis, was also observed during mineralization. We expect time-lapse Raman Imaging to help us to further elucidate osteoblastic mineralization mechanisms that have previously been unobservable.
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alkyne tag Raman Imaging for visualization of mobile small molecules in live cells
Journal of the American Chemical Society, 2012Co-Authors: Hiroyuki Yamakoshi, Kosuke Dodo, Almar F Palonpon, Jun Ando, Katsumasa Fujita, Satoshi Kawata, Mikiko SodeokaAbstract:Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous molecule in live cells. Here, we show that alkyne-tag Raman Imaging (ATRI) is a promising approach for visualizing nonimmobilized small molecules in live cells. An examination of structure–Raman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogues in live cells. Cellular concentrations of diyne-tagged CoQ analogues could be semiquantitatively estimated. Finally, simultaneous Imaging of two small molecules, 5-ethynyl-2′-deoxyuridine (EdU) and a CoQ analogue, with distinct Raman tags was demonstrated.
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near field Raman Imaging of organic molecules by an apertureless metallic probe scanning optical microscope
Journal of Chemical Physics, 2002Co-Authors: Norikiko Hayazawa, Yasushi Inouye, Zouheir Sekkat, Satoshi KawataAbstract:Near-field Raman Imaging of organic molecules is demonstrated by an apertureless near-field scanning optical microscope, the tip of which is a silver-layer-coated cantilever of an atomic force microscope (AFM). The virtue of the enhanced electric field at the tip apex due to the surface plasmon polariton excitations enhances the Raman scattering cross sections. This phenomenon allows us to reveal from near-field Raman images the molecular vibrational distributions of Rhodamine6G and Crystal Violet molecules beyond the diffraction limit of a light. These molecular vibrations cannot be distinguished by AFM topographic images.
Ronei J Poppi - One of the best experts on this subject based on the ideXlab platform.
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detection of malathion in food peels by surface enhanced Raman Imaging spectroscopy and multivariate curve resolution
Analytica Chimica Acta, 2015Co-Authors: Carlos Diego L De Albuquerque, Ronei J PoppiAbstract:An analytical methodology was developed for detection of malathion in the peels of tomatoes and Damson plums by surface-enhanced Raman Imaging spectroscopy and multivariate curve resolution. To recover the pure spectra and the distribution mapping of the analyzed surfaces, non-negative matrix factorization (NMF), multivariate curve calibration methods with alternating least squares (MCR-ALS) and MCR with weighted alternating least square (MCR-WALS) were utilized. Error covariance matrices were estimated to evaluate the structure of the error over all the data. For the tomato data, NMF-ALS and MCR-ALS presented excellent spectral recovery even in the absence of initial knowledge of the pesticide spectrum. For the Damson plum data, owing to heteroscedastic noise, MCR-WALS produced better results. This methodology enabled detection below to the maximum residue limit permitted for this pesticide. This approach can be implemented for in situ monitoring because it is fast and does not require extensive manipulation of samples, making its use feasible for other fruits and pesticides as well.
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Raman Imaging spectroscopic characterization of modified poly(dimethylsiloxane) for micro total analysis systems applications.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2012Co-Authors: Richard P. S. De Campos, Márcia Cristina Breitkreitz, Inez Valéria Pagotto Yoshida, Ronei J Poppi, José Alberto Fracassi Da SilvaAbstract:Methacryloxypropyl-modified poly(dimethylsiloxane) rubbers were obtained from poly(dimethylsiloxane), PDMS, and methacryloxypropyltrimethoxysilane, MPTMS, by polycondensation reactions. The modified rubbers, prepared with 20 and 30% (v/v) of MPTMS, were used as substrates for microchannel fabrication by the CO2 laser ablation technique. Raman Imaging spectroscopy was used for the surface characterization, showing the homogeneity of the rubbery material, with uniform distribution of the crosslinking centers. Under the experimental conditions used, damage to the rubber from the CO2 laser radiation used for the channel engraving was not observed. Correlation maps of the surface were obtained in order to spatially evaluate the modification inside and outside the channels. The correlations between the methacryloxypropyl-modified poly(dimethylsiloxane) rubbers and MPTMS (spectral range of 1800–1550 cm−1) and PDMS (spectral range of 820–670 cm−1) precursors were higher than 0.95 and 0.99, respectively. In addition, Raman Imaging spectroscopy allows monitoring the topography of the fabricated microchannel.
Hiroyuki Yamakoshi - One of the best experts on this subject based on the ideXlab platform.
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the alkyne tag Raman Imaging of coronatine a plant pathogen virulence factor in commelina communis and its possible mode of action
Organic and Biomolecular Chemistry, 2018Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Kengo Hayashi, Yasuhiro Ishimaru, Yousuke Takaoka, Mikiko SodeokaAbstract:We previously reported that coronatine, a virulence factor of plant bacteria, facilitates bacterial infection through an ER (endoplasmic reticulum)-mediated, non-canonical mechanism in the model dicot plant, Arabidopsis thaliana. Here, we report that this same ER-mechanism is ubiquitous among dicots and monocots, and works by affecting the ethylene signaling pathway widely found in plants. The subcellular localization of coronatine by the alkyne-tag Raman Imaging (ATRI) approach provided a convincing clue.
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noncanonical function of a small molecular virulence factor coronatine against plant immunity an in vivo Raman Imaging approach
ACS central science, 2017Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Yasuhiro Ishimaru, Yousuke Takaoka, Takeshi Nakano, Shinya Tsukiji, Mikiko SodeokaAbstract:Coronatine (1), a small-molecular virulence factor produced by plant-pathogenic bacteria, promotes bacterial infection by inducing the opening of stomatal pores, the major route of bacterial entry into the plant, via the jasmonate-mediated COI1-JAZ signaling pathway. However, this pathway is also important for multiple plant functions, including defense against wounding by herbivorous insects. Thus, suppression of the COI1-JAZ signaling pathway to block bacterial infection would concomitantly impair plant defense against herbivorous wounding. Here, we report additional, COI1-JAZ-independent, action of 1 in Arabidopsis thaliana guard cells. First, we found that a stereoisomer of 1 regulates the movement of Arabidopsis guard cells without affecting COI1-JAZ signaling. Second, we found using alkyne-tagged Raman Imaging (ATRI) that 1 is localized to the endoplasmic reticulum (ER) of living guard cells of Arabidopsis. The use of arc6 mutant lacking chloroplast formation was pivotal to circumvent the issue of a...
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Noncanonical Function of a Small-Molecular Virulence Factor Coronatine against Plant Immunity: An In Vivo Raman Imaging Approach
2017Co-Authors: Minoru Ueda, Hiroyuki Yamakoshi, Kosuke Dodo, Syusuke Egoshi, Yasuhiro Ishimaru, Yousuke Takaoka, Takeshi Nakano, Shinya Tsukiji, Mikiko SodeokaAbstract:Coronatine (1), a small-molecular virulence factor produced by plant-pathogenic bacteria, promotes bacterial infection by inducing the opening of stomatal pores, the major route of bacterial entry into the plant, via the jasmonate-mediated COI1-JAZ signaling pathway. However, this pathway is also important for multiple plant functions, including defense against wounding by herbivorous insects. Thus, suppression of the COI1-JAZ signaling pathway to block bacterial infection would concomitantly impair plant defense against herbivorous wounding. Here, we report additional, COI1-JAZ-independent, action of 1 in Arabidopsis thaliana guard cells. First, we found that a stereoisomer of 1 regulates the movement of Arabidopsis guard cells without affecting COI1-JAZ signaling. Second, we found using alkyne-tagged Raman Imaging (ATRI) that 1 is localized to the endoplasmic reticulum (ER) of living guard cells of Arabidopsis. The use of arc6 mutant lacking chloroplast formation was pivotal to circumvent the issue of autofluorescence during ATRI. These findings indicate that 1 has an ER-related action on Arabidopsis stomata that bypasses the COI1-JAZ signaling module. It may be possible to suppress the action of 1 on stomata without impairing plant defense responses against herbivores
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alkyne tag Raman Imaging for visualization of mobile small molecules in live cells
Journal of the American Chemical Society, 2012Co-Authors: Hiroyuki Yamakoshi, Kosuke Dodo, Almar F Palonpon, Jun Ando, Katsumasa Fujita, Satoshi Kawata, Mikiko SodeokaAbstract:Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous molecule in live cells. Here, we show that alkyne-tag Raman Imaging (ATRI) is a promising approach for visualizing nonimmobilized small molecules in live cells. An examination of structure–Raman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogues in live cells. Cellular concentrations of diyne-tagged CoQ analogues could be semiquantitatively estimated. Finally, simultaneous Imaging of two small molecules, 5-ethynyl-2′-deoxyuridine (EdU) and a CoQ analogue, with distinct Raman tags was demonstrated.