The Experts below are selected from a list of 1938 Experts worldwide ranked by ideXlab platform
Christophe De Champs - One of the best experts on this subject based on the ideXlab platform.
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Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Thomas Guillard, Anne-laure Lebreil, Lars Hestbjerg Hansen, Aymric Kisserli, Sibel Berger, Alain Lozniewski, Corentine Alauzet, Christophe De ChampsAbstract:We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.
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discrimination between native and tn6010 associated oqxab in klebsiella spp Raoultella spp and other enterobacteriaceae by using a two step strategy
Antimicrobial Agents and Chemotherapy, 2015Co-Authors: Thomas Guillard, Anne-laure Lebreil, Lars Hestbjerg Hansen, Aymric Kisserli, Sibel Berger, Alain Lozniewski, Corentine Alauzet, Christophe De ChampsAbstract:We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.
Eugenia Gospodarek-komkowska - One of the best experts on this subject based on the ideXlab platform.
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Raoultella spp. - reliable identification, susceptibility to antimicrobials and antibiotic resistance mechanisms.
Journal of medical microbiology, 2020Co-Authors: Alicja Sekowska, Tomasz Bogiel, Marcin Woźniak, Eugenia Gospodarek-komkowskaAbstract:Introduction. Raoultella spp. representatives are Gram-negative rod-shaped bacteria of the Enterobacteriaceae family. These bacteria are commonly found in the natural environment. Aim. The aim of the study was to indicate the reliable method for Raoultella spp. strains identification, evaluate the susceptibility of Raoultella spp. strains to selected antimicrobials and to detect their resistance mechanisms to beta-lactams. Methodology. Susceptibility of the strains to chosen antimicrobials was determined using the automatic method. The presence of particular antimicrobial resistant mechanism and genes encoding ESBLs and MBLs was determined respectively with double-disc synergy test and commercially available kit – eazyplex SuperBug CRE test (Amplex Diagnostics) and standard PCR. For the selected strains, DNA sequencing was performed. Results. Amongst 105 of the examined Raoultella spp. strains, majority were sensitive to: imipenem (99.0 %), meropenem (98.1 %), gentamicin (93.3 %) and ciprofloxacin (92.4 %). Of the tested Raoultella strains, thirteen (12.4 %) produced ESBLs and one strain simultaneously ESBLs and MBLs. The DNA sequencing results were as follows: for all the reference strains the correct species identification was achieved, for the analysed strains two were identified as R. planticola and one as R. ornithinolytica . Conclusion. Although Raoultella spp. strains remain sensitive to antibiotics, there is a constant need to monitor the sensitivity of these bacteria to selected antimicrobials. Isolation of a multi-drug resistant R. ornithinolytica strain indicates that even the less frequently isolated species of Enterobacteriaceae family should be precisely identified because they might be of clinical importance and the particular strain can also produce enzymes that pose the greatest threat today.
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Identification of Raoultella spp.: Comparison of three methods
Indian journal of medical microbiology, 2018Co-Authors: Alicja Sekowska, Agnieszka Mikucka, Eugenia Gospodarek-komkowskaAbstract:Background: Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Raoultella spp. is difficult due to a phylogenetic relationship with Klebsiella spp. Purpose: Available biochemical tests do not always allow for their identification to species. Thus, the aim of this study was to evaluate selected methods of identification of Raoultella spp. and their differentiation from genus Klebsiella. Materials and Methods: In this evaluation three methods were used such as manual test ID32E (bioMerieux), automatic test VITEK2 Compact (bioMerieux) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) method (Bruker). Results: Good identification of the species was obtained for 81.4% of the strains in the ID32E test, 93.3% in VITEK2 Compact test, and 97.4% in MALDI-TOF MS method, respectively. Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella.
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Identification of Raoultella spp.: Comparison of three methods
Wolters Kluwer Medknow Publications, 2018Co-Authors: Alicja Sekowska, Agnieszka Mikucka, Eugenia Gospodarek-komkowskaAbstract:Background: Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Raoultella spp. is difficult due to a phylogenetic relationship with Klebsiella spp. Purpose: Available biochemical tests do not always allow for their identification to species. Thus, the aim of this study was to evaluate selected methods of identification of Raoultella spp. and their differentiation from genus Klebsiella. Materials and Methods: In this evaluation three methods were used such as manual test ID32E (bioMérieux), automatic test VITEK2 Compact (bioMérieux) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) method (Bruker). Results: Good identification of the species was obtained for 81.4% of the strains in the ID32E test, 93.3% in VITEK2 Compact test, and 97.4% in MALDI-TOF MS method, respectively. Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella
Alicja Sekowska - One of the best experts on this subject based on the ideXlab platform.
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Raoultella spp. - reliable identification, susceptibility to antimicrobials and antibiotic resistance mechanisms.
Journal of medical microbiology, 2020Co-Authors: Alicja Sekowska, Tomasz Bogiel, Marcin Woźniak, Eugenia Gospodarek-komkowskaAbstract:Introduction. Raoultella spp. representatives are Gram-negative rod-shaped bacteria of the Enterobacteriaceae family. These bacteria are commonly found in the natural environment. Aim. The aim of the study was to indicate the reliable method for Raoultella spp. strains identification, evaluate the susceptibility of Raoultella spp. strains to selected antimicrobials and to detect their resistance mechanisms to beta-lactams. Methodology. Susceptibility of the strains to chosen antimicrobials was determined using the automatic method. The presence of particular antimicrobial resistant mechanism and genes encoding ESBLs and MBLs was determined respectively with double-disc synergy test and commercially available kit – eazyplex SuperBug CRE test (Amplex Diagnostics) and standard PCR. For the selected strains, DNA sequencing was performed. Results. Amongst 105 of the examined Raoultella spp. strains, majority were sensitive to: imipenem (99.0 %), meropenem (98.1 %), gentamicin (93.3 %) and ciprofloxacin (92.4 %). Of the tested Raoultella strains, thirteen (12.4 %) produced ESBLs and one strain simultaneously ESBLs and MBLs. The DNA sequencing results were as follows: for all the reference strains the correct species identification was achieved, for the analysed strains two were identified as R. planticola and one as R. ornithinolytica . Conclusion. Although Raoultella spp. strains remain sensitive to antibiotics, there is a constant need to monitor the sensitivity of these bacteria to selected antimicrobials. Isolation of a multi-drug resistant R. ornithinolytica strain indicates that even the less frequently isolated species of Enterobacteriaceae family should be precisely identified because they might be of clinical importance and the particular strain can also produce enzymes that pose the greatest threat today.
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The many faces of Raoultella spp.
Postępy Higieny i Medycyny Doświadczalnej, 2019Co-Authors: Alicja SekowskaAbstract:Raoultella genus consists of Gram-negative, aerobic, encapsulated and non-motile rods. The name of the genus derives from the name of the French bacteriologist Raoul. Currently, four species belong to the genus: R. planticola, R. ornithinolytica, R. terrigena and R. electrica. The standard biochemical test used to identify Raoultella genus should be supplemented with additional tests, because of the close relationship between the genera Raoultella and Klebsiella. In 2001 Klebsiella planticola, K. ornithinolytica and K. terrigena were re-classified to new genus Raoultella. Re-classification was based on 16S rRNA sequence and rpoB, gyrA and gyrB genes. An alternative to phenotypic identification may be mass spectrometry or genetic methods (16s rRNA). These bacteria are commonly associated with natural environments (plants, water, soil). Raoultella spp. rods are not a highly virulent pathogen. Their virulence factors include polysaccharide capsule, fimbriae, siderophores, toxins and ability to form a biofilm. It has been shown that Raoultella spp. may colonize the gastrointestinal and upper respiratory tract in humans and cause cholangitis and lung infections. The literature also includes works on the antimicrobial activity of Raoultella rods and the possibility of using them in the environment protection. This review summarizes the current knowledge of Raoultella species identification, virulence and the possibility of using them in the protection of the environment.
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Identification of Raoultella spp.: Comparison of three methods
Indian journal of medical microbiology, 2018Co-Authors: Alicja Sekowska, Agnieszka Mikucka, Eugenia Gospodarek-komkowskaAbstract:Background: Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Raoultella spp. is difficult due to a phylogenetic relationship with Klebsiella spp. Purpose: Available biochemical tests do not always allow for their identification to species. Thus, the aim of this study was to evaluate selected methods of identification of Raoultella spp. and their differentiation from genus Klebsiella. Materials and Methods: In this evaluation three methods were used such as manual test ID32E (bioMerieux), automatic test VITEK2 Compact (bioMerieux) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) method (Bruker). Results: Good identification of the species was obtained for 81.4% of the strains in the ID32E test, 93.3% in VITEK2 Compact test, and 97.4% in MALDI-TOF MS method, respectively. Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella.
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Identification of Raoultella spp.: Comparison of three methods
Wolters Kluwer Medknow Publications, 2018Co-Authors: Alicja Sekowska, Agnieszka Mikucka, Eugenia Gospodarek-komkowskaAbstract:Background: Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Raoultella spp. is difficult due to a phylogenetic relationship with Klebsiella spp. Purpose: Available biochemical tests do not always allow for their identification to species. Thus, the aim of this study was to evaluate selected methods of identification of Raoultella spp. and their differentiation from genus Klebsiella. Materials and Methods: In this evaluation three methods were used such as manual test ID32E (bioMérieux), automatic test VITEK2 Compact (bioMérieux) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) method (Bruker). Results: Good identification of the species was obtained for 81.4% of the strains in the ID32E test, 93.3% in VITEK2 Compact test, and 97.4% in MALDI-TOF MS method, respectively. Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella
Meizhen Zhong - One of the best experts on this subject based on the ideXlab platform.
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Neonatal septicemia caused by a rare pathogen: Raoultella planticola - a report of four cases
BMC infectious diseases, 2020Co-Authors: Xianrui Chen, Shaoqing Guo, Dengli Liu, Meizhen ZhongAbstract:Raoultella planticola(R.planticola) is a very rare opportunistic pathogen and sometimes even associated with fatal infection in pediatric cases. Recently,the emergence of carbapenem resistance strains are constantly being reported and a growing source of concern for pediatricians. We reported 4 cases of neonatal septicemia caused by Raoultella planticola. Their gestational age was 211 to 269 days, and their birth weight was 1490 to 3000 g.The R. planticola infections were detected on the 9th to 27th day after hospitalization and occured between May and June. They clinically manifested as poor mental response, recurrent cyanosis, apnea, decreased heart rate and blood oxygen, recurrent jaundice, fever or nonelevation of body temperature. The C-reactive protein and procalcitonin were elevated at significantly in the initial phase of the infection,and they had leukocytosis or leukopenia. Prior to R.planticola infection,all of them recevied at least one broad-spectrum antibiotic for 7-27d.All the R.planticola strains detected were only sensitive to amikacin, but resistant to other groups of drugs: cephalosporins (such as cefazolin, ceftetan,etc) and penicillins (such as ampicillin-sulbactam,piperacillin,etc),and even developed resistance to carbapenem. All the infants were clinically cured and discharged with overall good prognosis. Neonatal septicemia caused by Raoultella planticola mostly occured in hot and humid summer, which lack specific clinical manifestations. Pediatricians should keep in mind that R. planticola can be a potential source of neonatal sepsis and even has the potential to acquire carbapenem-resistance. Preventing outbreaks of epidemics requires early detection, timely diagnosis and treatment, and active isolation.
Diedre Hofinger - One of the best experts on this subject based on the ideXlab platform.
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A novel case of Raoultella planticola urinary tract infection.
Infection, 2012Co-Authors: D. S. Olson, Kingsley A. Asare, M. Lyons, Diedre HofingerAbstract:Raoultella species are Gram-negative, non-motile bacilli primarily considered to be environmental bacteria (Bagley et al.; Curr Microbiol 6:105–109, 1981). R. planticola has rarely been documented as a cause of human infections and has never been reported to cause urinary tract infections. We report the first case of R. planticola cystitis.
