The Experts below are selected from a list of 1077 Experts worldwide ranked by ideXlab platform
Rajenderan Vinothkumar - One of the best experts on this subject based on the ideXlab platform.
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chemopreventive effect of Zingerone against colon carcinogenesis induced by 1 2 dimethylhydrazine in rats
European Journal of Cancer Prevention, 2014Co-Authors: Rajenderan Vinothkumar, Mani Sudha, Namasivayam NaliniAbstract:Zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butane], one of the active phenolic components isolated from Zingiber officinale, has antioxidant and anticarcinogenic properties. In our study, we have evaluated the effect of different doses of Zingerone on lipid peroxidation (thiobarbituric acid-reactive
Qi-zhu Tang - One of the best experts on this subject based on the ideXlab platform.
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Zingerone attenuates aortic banding‐induced cardiac remodelling via activating the eNOS/Nrf2 pathway
Journal of Cellular and Molecular Medicine, 2019Co-Authors: Chen Liu, Zhulan Cai, Saiyang Xie, Mingxia Duan, Qingwen Xie, Yuan Yuan, Wei Deng, Qi-zhu TangAbstract:Cardiac remodelling refers to a series of changes in the size, shape, wall thickness and tissue structure of the ventricle because of myocardial injury or increased pressure load. Studies have shown that cardiac remodelling plays a significant role in the development of heart failure. Zingerone, a monomer component extracted from ginger, has been proven to possess various properties including antioxidant, anti-inflammatory, anticancer and antidiabetic properties. As oxidative stress and inflammation contribute to acute and chronic myocardial injury, we explored the role of Zingerone in cardiac remodelling. Mice were subjected to aortic banding (AB) or sham surgery and then received intragastric administration of Zingerone or saline for 25 days. In vitro, neonatal rat cardiomyocytes (NRCMs) were treated with Zingerone (50 and 250 μmol/L) when challenged with phenylephrine (PE). We observed that Zingerone effectively suppressed cardiac hypertrophy, fibrosis, oxidative stress and inflammation. Mechanistically, Zingerone enhanced the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/antioxidant response element (ARE) activation via increasing the phosphorylation of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production. Additionally, we used Nrf2-knockout (KO) and eNOS-KO mice and found that Nrf2 or eNOS deficiency counteracts these cardioprotective effects of Zingerone in vivo. Together, we concluded that Zingerone may be a potent treatment for cardiac remodelling that suppresses oxidative stress via the eNOS/Nrf2 pathway.
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Zingerone attenuates aortic banding induced cardiac remodelling via activating the enos nrf2 pathway
Journal of Cellular and Molecular Medicine, 2019Co-Authors: Chen Liu, Zhulan Cai, Saiyang Xie, Mingxia Duan, Qingwen Xie, Yuan Yuan, Wei Deng, Qi-zhu TangAbstract:Cardiac remodelling refers to a series of changes in the size, shape, wall thickness and tissue structure of the ventricle because of myocardial injury or increased pressure load. Studies have shown that cardiac remodelling plays a significant role in the development of heart failure. Zingerone, a monomer component extracted from ginger, has been proven to possess various properties including antioxidant, anti-inflammatory, anticancer and antidiabetic properties. As oxidative stress and inflammation contribute to acute and chronic myocardial injury, we explored the role of Zingerone in cardiac remodelling. Mice were subjected to aortic banding (AB) or sham surgery and then received intragastric administration of Zingerone or saline for 25 days. In vitro, neonatal rat cardiomyocytes (NRCMs) were treated with Zingerone (50 and 250 μmol/L) when challenged with phenylephrine (PE). We observed that Zingerone effectively suppressed cardiac hypertrophy, fibrosis, oxidative stress and inflammation. Mechanistically, Zingerone enhanced the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/antioxidant response element (ARE) activation via increasing the phosphorylation of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production. Additionally, we used Nrf2-knockout (KO) and eNOS-KO mice and found that Nrf2 or eNOS deficiency counteracts these cardioprotective effects of Zingerone in vivo. Together, we concluded that Zingerone may be a potent treatment for cardiac remodelling that suppresses oxidative stress via the eNOS/Nrf2 pathway.
Kusum Harjai - One of the best experts on this subject based on the ideXlab platform.
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Sustained release of Zingerone from polymeric nanoparticles: An anti-virulence strategy against Pseudomonas aeruginosa
Journal of Bioactive and Compatible Polymers, 2020Co-Authors: Karuna Sharma, Sanjay Chhibber, Pradip Nirbhavane, Kusum HarjaiAbstract:Zingerone loaded chitosan nanoparticles (Z-NPs) were developed to deliver the Zingerone across cell membrane and to further enhance its anti-virulence property. The Z-NPs were characterized with respect to size, percentage entrapment efficiency (% EE), zeta potential and percentage drug release. Further the Z-NPs were evaluated for antioxidant DPPH assay, antibiofilm, anti-virulence activities, and gene expression profiles. The developed Z-NPs showed an average size of 390 nm, zeta potential of +56.6 mV, 67% drug entrapment efficiency, and exhibited pH dependent controlled release of Zingerone over a period of 5 days (up to 80%). The Z-NPs retained the antioxidant effect of Zingerone as assessed by DPPH scavenging assay. Evaluation of nanoformulation for anti-virulence potential against Pseudomonas aeruginosa depicted significant reduction in swimming, swarming, and twitching motilities along with quorum sensing inhibition and eradication of biofilms. Decrease in expression of quorum sensing (QS) genes was also observed in the presence of Z-NPs. The results of the present study revealed that Z-NPs could be exploited as a promising anti-virulence candidate against P. aeruginosa infections.
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Zingerone Suppresses Liver Inflammation Induced by Antibiotic Mediated Endotoxemia through Down Regulating Hepatic mRNA Expression of Inflammatory Markers in Pseudomonas aeruginosa Peritonitis Mouse Model
2016Co-Authors: Lokender Kumar, Sanjay Chhibber, Kusum HarjaiAbstract:Antibiotic-induced endotoxin release is associated with high mortality rate even when appropriate antibiotics are used for the treatment of severe infections in intensive care units. Since liver is involved in systemic clearance and detoxification of endotoxin hence it becomes a primary target organ for endotoxin mediated inflammation. Currently available anti-inflammatory drugs give rise to serious side effects. Hence, there is an urgent need for safe and effective anti-inflammatory therapy. It is likely that anti-inflammatory phytochemicals and neutraceutical agents may have the potential to reduce the endotoxin mediated inflammation and complications associated with endotoxin release. Keeping this in mind, the present study was planned to evaluate the hepatoprotective potential of Zingerone (active compound of zingiber officinale) against liver inflammation induced by antibiotic mediated endotoxemia. The selected antibiotics capable of releasing high content of endotoxin were employed for their in vivo efficacy in P.aeruginosa peritonitis model. Released endotoxin induced inflammation and Zingerone as co-anti-inflammatory therapy significantly reduced inflammatory response. Improved liver histology and reduced inflammatory markers MDA, RNI, MPO, tissue damage markers (AST, ALT, ALP) and inflammatory cytokines (MIP-2, IL-6 and TNF-a) were indicative of therapeutic potential of Zingerone. The mechanism of action of Zingerone may be related to significant inhibition of the mRNA expression of inflammatory markers (TLR4, RelA, NF-kB2, TNF- a, iNOS, COX-2) indicating that Zingerone interferes with cell signalling pathway and suppresses hyper expression o
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American Journal of Phytomedicine and Clinical Therapeutics www.ajpct.org Original Article Recent Update on Multiple Pharmacological Benefits of Zingerone: A Quick Review
2015Co-Authors: Lokender Kumar, Kusum Harjai, Sanjay ChhibberAbstract:Anti-inflammatory, anti-oxidant, anti-cancerous, radioprotective and anti-microbial properties of ginger (Zingiber officinale) rhizome are well recognized worldwide. Ginger consumption also helps in the management of inflammatory damage in Alzheimer’s and Parkinson’s disease. Biological properties of ginger rhizome are attributed to its active compounds including, gingerols, shogaols, paradols and Zingerone. Zingerone is active component of dried ginger rhizome and used in food industry as flavouring agent. Various observations on the protective role of Zingerone against radiation induced oxidative stress; tissue inflammatory damage and drug induced toxicity have been made using a wide range of experimental models. Mechanism of antioxidant and anti-inflammatory action of Zingerone has been linked to potent radical scavenging effect, causing alteration in gene/protein expression and interference with intracellular cell signalling pathways of inflammation. Our laboratory for the first time revealed antibiofilm activity of Zingerone against opportunistic pathogen Pseudomonas aeruginosa. This study suggested that Zingerone can be explored as a safe anti-infective agent against P. aeruginosa biofilm associated infections. Hence, comprehensive information showing health benefits of Zingerone are urgently needed that may provide an update on its therapeutic potential. We conducted a detailed search of literature to summarize the proven health benefits and biological properties of Zingerone. This review article summarizes in detail the in vitro and in vivo pharmacological benefits of Zingerone
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Zingerone silences quorum sensing and attenuates virulence of Pseudomonas aeruginosa
Fitoterapia, 2015Co-Authors: Lokender Kumar, Sanjay Chhibber, Rajnish Kumar, Manoj Kumar, Kusum HarjaiAbstract:Quorum sensing in Pseudomonas aeruginosa plays an imperative role in virulence factor, biofilm formation and antimicrobial resistance. Blocking quorum sensing pathways are viewed as viable anti-virulent therapy in association with traditional antimicrobial therapy. Anti-quorum sensing dietary phytochemicals with may prove to be a safe and viable choice as anti-virulent drug candidates. Previously, our lab proved Zingerone as potent anti-biofilm agent hence; further its anti-virulent and anti-quorum activities were evaluated. Zingerone, besides decreasing swimming, swarming and twitching phenotypes of P. aeruginosa PAO1, reduced biofilm forming capacity and production of virulence factors including rhamnolipid, elastase, protease, pyocyanin, cell free and cell bound hemolysin (p < 0.001) indicating anti-virulent property attributing towards attenuation of virulence of P. aeruginosa. Further Zingerone not only had marked effect on the production of quorum sensing signal molecules by clinical isolates of P. aeruginosa but also showed significant interference with the activation of QS reporter strains. To study the mechanism of blocking quorum sensing cascade, in silico analysis was carried out. Anti-QS activity was attributed to interference with the ligand receptor interaction of Zingerone with QS receptors (TraR, LasR, RhlR and PqsR). Zingerone showed a good comparative docking score to respective autoinducer molecules which was even higher than that of vanillin, a proven anti-quorum sensing phytochemical. The results of the present study revealed the anti-quorum sensing activity of Zingerone targeting ligand–receptor interaction, hence proposing Zingerone as a suitable anti-virulent drug candidate against P. aeruginosa infections.
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Structural Alterations in Pseudomonas Aeruginosa by Zingerone Contribute to Enhanced Susceptibility to Antibiotics, Serum and Phagocytes
Life Sciences, 2014Co-Authors: Lokender Kumar, Sanjay Chhibber, Kusum HarjaiAbstract:Abstract Aims Excessive use of antibiotics has led to evolutionary adaptation resulting in emergence of multidrug resistance in P. aeruginosa. The aim of the present study was oriented towards exploiting Zingerone (active component of ginger) in making P. aeruginosa more susceptible to killing with antibiotics, humoral/cellular defences and studying its underlying mechanism. Main method Effect of Zingerone treatment on antibiotic susceptibility, serum, and phagocytic killing of P. aeruginosa was studied. The underlying mechanism was evaluated in terms of cell surface hydrophobicity, alginate and LPS production. TNF-α and MIP-2 cytokine production by mouse macrophages was also checked. Structural analysis was carried out using scanning electron microscopy (SEM) and liquid chromatography-mass spectrometry (LC-MS) analysis. Key findings Zingerone treated cells showed increased susceptibility to variety of antibiotics, serum as well as macrophages (p P. aeruginosa which contributes towards enhanced susceptibility to antibiotics and innate immune system. Significance Use of phytochemicals may prove to be a novel therapeutic approach by enhancing susceptibility of pathogenic microorganisms to antibiotics and immune system. Zingerone has proved to be one such agent which can be employed as a potential anti-virulent drug candidate against P. aeruginosa infections.
Mohammad Firoz Alam - One of the best experts on this subject based on the ideXlab platform.
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Zingerone ameliorates tellurium induced nephrotoxicity by abating elevated serum markers in the rats
Environment Conservation Journal, 2020Co-Authors: Mohammad Firoz Alam, Saeed Alshahrani, Essam Alamir, Mohammad Abdurrhman Alhazmi, Tarique Anwer, Gyas Khan, Moni Sivakumar SivagurunathanAbstract:The present study was designed to investigate the nephrotoxicity of tellurium (Sodium tellurite) in rats through evaluating the level of kidney functional marker enzymes and its treatment with Zingerone. Rats were divided into four groups, Group-A (control group), Group-B (tellurium treated group), Group-C (tellurium + Zingerone treatment group), and Group-D (Zingerone treatment alone) and each group have six animals. Tellurium was given in Group-B and Group-C at the dose of 8.3mg/kg bodyweight daily orally for 15 days, while Zingerone of 100mg/kg body weight was given in Group-C as pre- and post-treatment orally for 15days. Group-D was given alone Zingerone of 100mg/kg bodyweight; orally for 15 days. Results revealed that tellurium administration significantly (P<0.001) increased the serum markers (ALP, BUN, Uric Acid and Creatinine) in Group-B as a compared to Group-A while the treatment with Zingerone significantly (P<0.001) decreased these elevated serum markers in Group-C as comparison to Group-B. There were no changes observed in the positive control (Zingerone administered Group-D). Thus, the present finding confirmed that the Zingerone plays a potential role in reducing nephrotoxicity against tellurium by abating elevated serum markers in rats.
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Zingerone ameliorates tellurium induced nephrotoxicity by abating elevated serum markers in the rats
Environment Conservation Journal, 2020Co-Authors: Mohammad Firoz Alam, Saeed Alshahrani, Essam Alamir, Mohammad Abdurrhman Alhazmi, Tarique Anwer, Gyas Khan, Moni Sivakumar SivagurunathanAbstract:The present study was designed to investigate the nephrotoxicity of tellurium (Sodium tellurite) in rats through evaluating the level of kidney functional marker enzymes and its treatment with Zingerone. Rats were divided into four groups, Group-A (control group), Group-B (tellurium treated group), Group-C (tellurium + Zingerone treatment group), and Group-D (Zingerone treatment alone) and each group have six animals. Tellurium was given in Group-B and Group-C at the dose of 8.3mg/kg bodyweight daily orally for 15 days, while Zingerone of 100mg/kg body weight was given in Group-C as pre- and post-treatment orally for 15days. Group-D was given alone Zingerone of 100mg/kg bodyweight; orally for 15 days. Results revealed that tellurium administration significantly (P
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Neuroprotective effects of Zingerone against carbon tetrachloride (CCl4) induced brain mitochondrial toxicity in Swiss albino mice
Journal of Applied and Natural Science, 2018Co-Authors: Mohammad Firoz AlamAbstract:The present study targeted the brain mitochondria dysfunction in Swiss albino mice through carbon tetrachloride intoxication and its treatment with Zingerone. It is proposed that brain mitochondria is the main organelle responsible for oxidative stress by producing reactive oxygen species (ROS). Swiss albino mice were divided into four groups; Group-1 was control; Group-2 was carbon tetrachloride (CCl4) toxic (1.5mg kg-1 bm i.p two days in a week.); Group-3 was pretreated with Zingerone (100 mg kg-1 b.m) a day before the administration of CCl4 and Group-4 was only Zingerone (100 mg kg-1 bm) given orally for 15days once in a day. At the end of the experiment mice were sacrificed and mitochondria were isolated from brain. Isolated brain mitochondria were further analyzed for oxidative stress marker. Thiobarbituric acid reactive substance (TBARS) content was increased significantly by CCl4 administration in Group-II as compared to the control Group-I, while the antioxidant (GSH) and other antioxidant enzyme GPx , GR, and CAT was depleted significantly in CCl4 treated Group-II as compare to control Group-I. Zingerone protected the toxicity of brain mitochondria by reducing the lipid peroxidation and enhancing the antioxidant enzyme in Group-III and there was no significant changes were noticed in Group-IV as compared to Group-I. Overall results showed the potential effects of Zingerone in protecting the neuronal cell loss by oxidative stress. Thus, the present study indicated that the Zingerone may be used as the potential therapeutic tools for the prevention of CCl4 induced brain mitochondrial toxicity.
Muneeb U. Rehman - One of the best experts on this subject based on the ideXlab platform.
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Zingerone prevents lead-induced toxicity in liver and kidney tissues by regulating the oxidative damage in Wistar rats.
Journal of Food Biochemistry, 2020Co-Authors: Insha Amin, Bilal Ahmad, Muneeb U. Rehman, Showkeen Muzamil, Ishraq Hussain, Bilal Ahmad Mir, Showkat Ahmad Ganaie, Manzoor R. Mir, Syed Shanaz, Azher ArafahAbstract:Among the heavy metal poisonings, lead is considered as a major toxic metal causing hematological, neurological, immunological, hepatic, and renal dysfunctions. Lead causes inhibition of ALAD leading to the ALA accumulation inside the cells. Lead also leads to disruption of the anti-oxidative enzyme system, organ function, and lipid membranes of the cell causing oxidative stress. Zingerone, a phenolic alkanone, is an active edible ingredient present in the ginger that possess varied pharmacological properties. The aim of our study was to evaluate the protective effect of Zingerone in lead-induced toxicity in wistar rats. ALAD concentration was improved in kidney and liver tissues treated with Zingerone. Protective effect of Zingerone was observed in terms of significant improvement in kidney and liver histology, anti-oxidant enzyme activity (CAT, SOD, GPx, and GR), organ function parameters, lipid profile, and decreased level of LPO. Therefore, Zingerone pretreatment can be a promising agent for alleviation of lead-induced oxidative damage in cells. PRACTICAL APPLICATIONS: Published reports have revealed that consumption of certain bioactive nutrients for example, flavonoids, mineral elements, and vitamins can offer defense from the environmental lead contamination. Zingerone is a strong anti-oxidant, with very less side effects and has exceptional property of scavenging free radicals, hence reducing the oxidative stresses. This fundamental property of Zingerone can alone help in countering the heavy metal toxicity. Different groups have published reported numerous properties of Zingerone but as per our understanding till date no study about alleviation of lead toxicity by Zingerone in animal model has been undertaken. Hence, we conducted this research to explore the preventive effect of Zingerone in lead induced kidney and liver toxicity. The outcome of our study shows potent anti-oxidant effect and ALAD modulatory property of Zingerone which makes it suitable edible candidate for use in countering lead toxicity.
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Chemopreventive efficacy Zingerone (4-[4-hydroxy-3-methylphenyl] butan-2-one) in experimental colon carcinogenesis in Wistar rats.
Environmental Toxicology, 2019Co-Authors: Majid Ahmad Ganaie, Muneeb U. Rehman, Abdulaziz Al Saeedan, Hassan Madhkali, Basit Lateef Jan, Tanvir Khatlani, Ishfaq Ahmad Sheikh, Khalida WaniAbstract:Colorectal cancer is one of the most common cancers worldwide. Development of naturally occurring inexpensive and safe alternatives can be effective in suppressing colon related proliferations. Zingerone (4-[4-hydroxy-3-methylphenyl] butan-2-one), a polyphenolic alkanone of ginger, has massive pharmacological properties and thus can be used as promising candidate against various ailments. In the current study, we aimed at demonstrating the protective effect of Zingerone against experimental colon carcinogenesis and elucidating its possible mechanism by studying inflammatory and Nrf-2 signaling cascade. Four groups of animals (I-IV) were made with six animals each. Group I (control) was given normal saline orally. Group II was given 1,2-dimethylhydrazine (DMH) at the dose rate of 20 mg/kg body weight. Group III and IV were treated with DMH at the dose rate of 20 mg/kg body weight and also received oral treatment of Zingerone at a dose rate of 50 and 100 mg/kg body weight, respectively, for first 5 weeks and animals were euthanized after 16 weeks. Our results reveal that DMH treated rats exhibited elevated ROS and MDA levels, increased activity of cytochrome P450 2E1 and serum marker enzyme carcinoembreyonic antigen (CEA), increased no of aberrant crypts of foci (ACF), and elevated expression of inflammatory and proliferative proteins. Nrf-2 was downregulated by DMH treatment. Treatment with Zingerone to DMH treated rats, resulted in alterations in the activity of the cytochrome P450 2E1 and CEA. In addition, immunostaining of NF-kB-p65, COX-2, iNOS, and PCNA, Ki-67 was suppressed by Zingerone. Furthermore, Zingerone administration also attenuated the level of IL-6 and TNF-? and it also helps in preserving mucous layer. Thus, Zingerone could be considered as a good chemopreventive agent in experimental model of colon carcinogenesis. Further studies are required to study other pathways involved in colon carcinogenesis and their modulation buy Zingerone.
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Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) protects against alloxan-induced diabetes via alleviation of oxidative stress and inflammation: Probable role of NF-kB activation.
Journal of The Saudi Pharmaceutical Society, 2018Co-Authors: Bilal Ahmad, Muneeb U. Rehman, Insha Amin, Adil Farooq, Showkeen Muzamil, Ishraq Hussain, Mubashir Masoodi, Manzoor R. Mir, Bilques FatimaAbstract:Abstract Diabetes is considered as the most common metabolic disease affecting millions of people all around the world. Use of natural herbal medicines can be effective in treating diabetes. Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) a polyphenolic alkanone extracted from ginger has a broad spectrum of pharmacological properties and thus can be used as a promising candidate against various ailments. In the current study we aimed at demonstrating the protective effect of Zingerone against diabetes mellitus and elucidating its possible mechanism. Five groups of animals (I-V) were made with ten animals each. Group I (control) was given normal saline orally. Group II (diabetic positive control) was given alloxan at the dose rate of 100 mg/kg bwt once. Group III and IV was given alloxan once at the dose rate of 100 mg/kg bwt. and received oral treatment of Zingerone at a dose rate of 50 and 100 mg/kg bwt respectively daily for 21 days. Group V was given alloxan at the dose of 100 mg/kg bwt. and was treated with standard drug glibenclamide at the dose rate of 4.5 mg/kg bwt. daily for 21 days. According to our findings we confirmed that Zingerone restrained the alloxan induced oxidative stress by increasing the activity of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and reducing the peroxidative damage. We also confirmed that Zingerone suppressed the level of redox sensitive transcription factor NFκB and downregulated other downstream inflammatory cytokines like interleukins (IL1-β IL-2, IL-6) and tumor necrosis factor alpha (TNF-α). Moreover, the experimental findings suggested that Zingerone improved the insulin levels. Taken together our results indicated that Zingerone effectively ameliorated the diabetes induced complications which provide a strong theoretical basis for Zingerone to be used clinically for treatment of diabetes.
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Chemoprotective potential of Zingerone (vanillyl acetone) in cyclophosphamide-induced hepatic toxicity
Pharmacognosy Magazine, 2018Co-Authors: Bilal Ahmad Mir, Muneeb U. Rehman, Insha Amin, Bilques Fatima, Rahil Razak, Aarif Ali, Omer Khalil Baba, Rayeesa Ali, S. Bilal, Showkeen MuzamilAbstract:Introduction: Cancer is one of the lethal diseases in the global world. Proliferation of cancer cells is commonly inhibited by chemotherapeutics. Cyclophosphamide (CP) is an alkylating chemotherapeutic agent often used for treatment of various types of cancers, but it is full of side effects which in turn lead to organ toxicity. Zingerone, a polyphenolic alkanone found in ginger, has strong antioxidant potential and causes extensive scavenging of free radicals and offers defense against oxidative stress. Twenty-four adult male Wistar rats were divided into four groups, six rats in each group. Materials and Methods: Group I (control), Group II (CP, 2 mg/kg bwt), Group III (cotreatment with Zingerone at the dose of 50 mg/kg bwt and CP at the dose of 2 mg/kg bwt), and Group IV (pretreatment of Zingerone at the dose of 50 mg/kg bwt for 7 days and CP at the dose of 2 mg/kg bwt for next 7 days). Results: CP significantly increased the level of hepatic marker enzymes such as alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase, drastically caused alteration in lipid profile and deficiency in antioxidant defense mechanism by decreasing the activities of antioxidant enzymes such as catalase, glutathione, glutathione-S-transferase, and glutathione peroxidase. This was accompanied by subsequent increase in lipid peroxidation, nitrite production, and marked DNA damage. Conclusion: The restoration of hepatic markers, amelioration of lipid profile, and improvement of antioxidant status and DNA damage by pre- and co-treatment with Zingerone clearly indicate the ameliorative potential of Zingerone against CP-induced organ toxicity and oxidative stress. The protective potential of Zingerone may be attributed to its strong antioxidant activity. Abbreviations used: CP: Cyclophosphamide; ALT: Alanine transaminase; AST: Aspartate transaminase; ALP: Alkaline phosphatase; CAT: Catalase; GSH: Reduced glutathione; ROS: Reactive oxygen species; SOD: Superoxide dismutase; LPO: lipid peroxidation; MDA: Malonaldehyde; GPX: Glutathione peroxidase.
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Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) protects against alloxan-induced diabetes via alleviation of oxidative stress and inflammation: Probable role of NF-kB activation
Elsevier, 2018Co-Authors: Bilal Ahmad, Muneeb U. Rehman, Insha Amin, Manzoor Ur Rahman Mir, Sheikh Bilal Ahmad, Adil Farooq, Showkeen Muzamil, Ishraq Hussain, Mubashir Masoodi, Bilques FatimaAbstract:Diabetes is considered as the most common metabolic disease affecting millions of people all around the world. Use of natural herbal medicines can be effective in treating diabetes. Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) a polyphenolic alkanone extracted from ginger has a broad spectrum of pharmacological properties and thus can be used as a promising candidate against various ailments. In the current study we aimed at demonstrating the protective effect of Zingerone against diabetes mellitus and elucidating its possible mechanism. Five groups of animals (I-V) were made with ten animals each. Group I (control) was given normal saline orally. Group II (diabetic positive control) was given alloxan at the dose rate of 100 mg/kg bwt once. Group III and IV was given alloxan once at the dose rate of 100 mg/kg bwt. and received oral treatment of Zingerone at a dose rate of 50 and 100 mg/kg bwt respectively daily for 21 days. Group V was given alloxan at the dose of 100 mg/kg bwt. and was treated with standard drug glibenclamide at the dose rate of 4.5 mg/kg bwt. daily for 21 days. According to our findings we confirmed that Zingerone restrained the alloxan induced oxidative stress by increasing the activity of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and reducing the peroxidative damage. We also confirmed that Zingerone suppressed the level of redox sensitive transcription factor NFκB and downregulated other downstream inflammatory cytokines like interleukins (IL1-β IL-2, IL-6) and tumor necrosis factor alpha (TNF-α). Moreover, the experimental findings suggested that Zingerone improved the insulin levels. Taken together our results indicated that Zingerone effectively ameliorated the diabetes induced complications which provide a strong theoretical basis for Zingerone to be used clinically for treatment of diabetes. Keywords: Alloxan, Diabetes, NFκB, Cytokines, Zingeron
