The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Hiroshi Kurata - One of the best experts on this subject based on the ideXlab platform.
-
Automatic evaluation of antifungal volatile compounds on the basis of the dynamic growth process of a single hypha
Applied microbiology and biotechnology, 1993Co-Authors: Hideaki Matsuoka, Osao Sumita, Kosuke Takatori, Hiroshi KurataAbstract:An automatic analysing system was developed and employed for the evaluation of antifungal activity of volatile compounds in the gas phase. Aspergillus niger was inoculated on agar medium in the Reaction Vessel. The Reaction Vessel was incubated at 28° C for 24 h and then a volatile compound was introduced into the Vessel either in a batch or flow manner. The antifungal activity of the respective compounds estimated in situ was expressed by the dynamic response parameters of a single hypha. All volatiles tested in the present system inhibited hyphal growth, except linalyl acetate: Limone and geraniol were the most inhibitory. In contrast, linalyl acetate promoted hyphal growth. By definition of the parameters, the fungicidal and fungistatic effects could be distinguished.
-
Evaluation of antifungal activity of antimycotics by automatic analyzing system
Mycopathologia, 1992Co-Authors: Hideaki Matsuoka, Osao Sumita, Kosuke Takatori, Hiroshi KurataAbstract:Antifungal activity of several antimycotics has been evaluated using an automatic analyzing system (AAS), which is composed of a specially designed Reaction Vessel, microscopic observation system, image analyzing system, and computer program for automatic tracing of hypha growth. The agar plate was prepared on the ceiling of the Reaction Vessel, and spore mass of a fungus ( Aspergillus niger ) was inoculated onto it. After the preincubation at 28 °C for 24 h the Reaction Vessel was set on a microscope stage and connected to the liquid flow system. An appropriate hypha was selected for the measurement of growth process during the following steps: first contact with saline for 30 min for the adaptation, the second contact with same saline for 30 min, contact with saline containing an antimycotic substance for 60 min, and contact with flushing saline for 60 min. During a sequence of these steps, the apical tip of a growing hypha displayed on a TV monitor was followed automatically. The dynamic response of hypha to an agent was analyzed by several parameters. Morphological changes of the hypha caused by respective agents were recorded on VTR for further analysis. By using this system, the antifungal activity of antimycotics could be quantitatively determined within several hours.
-
Automatic antifungal activity analyzing system on the basis of dynamic growth process of a single hypha
Mycopathologia, 1992Co-Authors: Satoru Yamada, Osao Sumita, Hiroshi Kurata, Jinan Cao, Kikuo Kurasawa, Hideaki MatsuokaAbstract:A system for the evaluation of antifungal activity of volatile compounds has been developed that is based on dynamic growth of a single hypha. The newly developed system is composed of a Reaction Vessel under a microscope, automatic stage, charge coupled device (CCD) camera, TV monitor, video tape recorder (VTR), and a microcomputer. A fungus was inoculated in the Reaction Vessel containing agar medium and then was treated with an antifungal reagent in the gas phase either in batch or flow Reaction manner. The apex of a growing hypha displayed on a TV monitor was followed automatically. From the ratio of the growth rate under exposure of a reagent (U_EXPO) to the growth rate before the exposure (U_PRE), the antifungal activity was expressed quantitatively.
Jianfeng Ping - One of the best experts on this subject based on the ideXlab platform.
-
contamination free visual detection of camv35s promoter amplicon using crispr cas12a coupled with a designed Reaction Vessel rapid specific and sensitive
Analytica Chimica Acta, 2020Co-Authors: Fang Zhang, Jianfeng PingAbstract:Abstract An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed Reaction Vessel. Genetically modified (GM) soybean (Roundup Ready®) powders containing CaMV35S promoter were employed as detection targets, which were amplified by loop-mediated isothermal amplification (LAMP). The CRISPR/Cas12a system directly mixed with amplified products at 37 °C for 5 min and detection results could be clearly identified by the naked eye under UV light (254 nm). A designed Reaction Vessel was employed to make operation easier and could effectively prevent contamination at the source. The CRISPR/Cas12a detection system was optimized in our study and the concentration of magnesium ions was proved to be important for the work of CRISPR/Cas12a system. The optimized concentration range of magnesium ions was between 10 mM and 12 mM. Besides, the activated Bst DNA polymerase also had little effects on CRISPR/Cas12a system. The developed method could significantly distinguish the specific and non-specific amplification. And as low as 0.05% transgenic contents in soybean powders could be detected. It would have the potential to be complementary to instrument-based ultrahigh sensitive method and provide a new solution for on-site rapid detection.
-
Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed Reaction Vessel: Rapid, specific and sensitive.
Analytica chimica acta, 2019Co-Authors: Fang Zhang, Jianfeng PingAbstract:Abstract An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed Reaction Vessel. Genetically modified (GM) soybean (Roundup Ready®) powders containing CaMV35S promoter were employed as detection targets, which were amplified by loop-mediated isothermal amplification (LAMP). The CRISPR/Cas12a system directly mixed with amplified products at 37 °C for 5 min and detection results could be clearly identified by the naked eye under UV light (254 nm). A designed Reaction Vessel was employed to make operation easier and could effectively prevent contamination at the source. The CRISPR/Cas12a detection system was optimized in our study and the concentration of magnesium ions was proved to be important for the work of CRISPR/Cas12a system. The optimized concentration range of magnesium ions was between 10 mM and 12 mM. Besides, the activated Bst DNA polymerase also had little effects on CRISPR/Cas12a system. The developed method could significantly distinguish the specific and non-specific amplification. And as low as 0.05% transgenic contents in soybean powders could be detected. It would have the potential to be complementary to instrument-based ultrahigh sensitive method and provide a new solution for on-site rapid detection.
Hideaki Matsuoka - One of the best experts on this subject based on the ideXlab platform.
-
Automatic evaluation of antifungal volatile compounds on the basis of the dynamic growth process of a single hypha
Applied microbiology and biotechnology, 1993Co-Authors: Hideaki Matsuoka, Osao Sumita, Kosuke Takatori, Hiroshi KurataAbstract:An automatic analysing system was developed and employed for the evaluation of antifungal activity of volatile compounds in the gas phase. Aspergillus niger was inoculated on agar medium in the Reaction Vessel. The Reaction Vessel was incubated at 28° C for 24 h and then a volatile compound was introduced into the Vessel either in a batch or flow manner. The antifungal activity of the respective compounds estimated in situ was expressed by the dynamic response parameters of a single hypha. All volatiles tested in the present system inhibited hyphal growth, except linalyl acetate: Limone and geraniol were the most inhibitory. In contrast, linalyl acetate promoted hyphal growth. By definition of the parameters, the fungicidal and fungistatic effects could be distinguished.
-
Evaluation of antifungal activity of antimycotics by automatic analyzing system
Mycopathologia, 1992Co-Authors: Hideaki Matsuoka, Osao Sumita, Kosuke Takatori, Hiroshi KurataAbstract:Antifungal activity of several antimycotics has been evaluated using an automatic analyzing system (AAS), which is composed of a specially designed Reaction Vessel, microscopic observation system, image analyzing system, and computer program for automatic tracing of hypha growth. The agar plate was prepared on the ceiling of the Reaction Vessel, and spore mass of a fungus ( Aspergillus niger ) was inoculated onto it. After the preincubation at 28 °C for 24 h the Reaction Vessel was set on a microscope stage and connected to the liquid flow system. An appropriate hypha was selected for the measurement of growth process during the following steps: first contact with saline for 30 min for the adaptation, the second contact with same saline for 30 min, contact with saline containing an antimycotic substance for 60 min, and contact with flushing saline for 60 min. During a sequence of these steps, the apical tip of a growing hypha displayed on a TV monitor was followed automatically. The dynamic response of hypha to an agent was analyzed by several parameters. Morphological changes of the hypha caused by respective agents were recorded on VTR for further analysis. By using this system, the antifungal activity of antimycotics could be quantitatively determined within several hours.
-
Automatic antifungal activity analyzing system on the basis of dynamic growth process of a single hypha
Mycopathologia, 1992Co-Authors: Satoru Yamada, Osao Sumita, Hiroshi Kurata, Jinan Cao, Kikuo Kurasawa, Hideaki MatsuokaAbstract:A system for the evaluation of antifungal activity of volatile compounds has been developed that is based on dynamic growth of a single hypha. The newly developed system is composed of a Reaction Vessel under a microscope, automatic stage, charge coupled device (CCD) camera, TV monitor, video tape recorder (VTR), and a microcomputer. A fungus was inoculated in the Reaction Vessel containing agar medium and then was treated with an antifungal reagent in the gas phase either in batch or flow Reaction manner. The apex of a growing hypha displayed on a TV monitor was followed automatically. From the ratio of the growth rate under exposure of a reagent (U_EXPO) to the growth rate before the exposure (U_PRE), the antifungal activity was expressed quantitatively.
Jonathan Brauer - One of the best experts on this subject based on the ideXlab platform.
-
thermal decomposition pyrolysis of urea in an open Reaction Vessel
Thermochimica Acta, 2004Co-Authors: Peter M Schaber, James Colson, Steven Higgins, Daniel Thielen, Bill Anspach, Jonathan BrauerAbstract:A study was done of the thermal decomposition of urea under open Reaction Vessel conditions by thermogravimetric analysis (TGA), high performance liquid chromatography (HPLC), Fourier transform-infrared (FT-IR), and an ammonium ion-selective electrode (ISE). Both evolved gases and urea residue were analyzed, and profiles of substances present versus temperature are given. Major Reaction intermediates are also identified. Plausible Reaction schemes based on product distribution in relation to temperature are proposed. Our data indicate that at temperatures in excess of 190 ◦ C, cyanuric acid (CYA), ammelide and ammeline are produced primarily from biuret. Biuret itself is a result of prior Reaction of cyanic acid, HNCO, with intact urea. Cyanic acid is primarily a result of urea decomposition at temperatures in excess of 152 ◦ C. CYA and ammelide first appear at approximately 175 ◦ C, but the Reaction rate is very slow. At temperatures in excess of 193 ◦ C, alternate Reactions involving the decomposition of biuret substantially increases Reaction rates. Several parallel processes compete for the production of products. Production of CYA, ammeline and ammelide appears complete at 250 ◦ C, after which sublimation and eventual
Fang Zhang - One of the best experts on this subject based on the ideXlab platform.
-
contamination free visual detection of camv35s promoter amplicon using crispr cas12a coupled with a designed Reaction Vessel rapid specific and sensitive
Analytica Chimica Acta, 2020Co-Authors: Fang Zhang, Jianfeng PingAbstract:Abstract An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed Reaction Vessel. Genetically modified (GM) soybean (Roundup Ready®) powders containing CaMV35S promoter were employed as detection targets, which were amplified by loop-mediated isothermal amplification (LAMP). The CRISPR/Cas12a system directly mixed with amplified products at 37 °C for 5 min and detection results could be clearly identified by the naked eye under UV light (254 nm). A designed Reaction Vessel was employed to make operation easier and could effectively prevent contamination at the source. The CRISPR/Cas12a detection system was optimized in our study and the concentration of magnesium ions was proved to be important for the work of CRISPR/Cas12a system. The optimized concentration range of magnesium ions was between 10 mM and 12 mM. Besides, the activated Bst DNA polymerase also had little effects on CRISPR/Cas12a system. The developed method could significantly distinguish the specific and non-specific amplification. And as low as 0.05% transgenic contents in soybean powders could be detected. It would have the potential to be complementary to instrument-based ultrahigh sensitive method and provide a new solution for on-site rapid detection.
-
Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed Reaction Vessel: Rapid, specific and sensitive.
Analytica chimica acta, 2019Co-Authors: Fang Zhang, Jianfeng PingAbstract:Abstract An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed Reaction Vessel. Genetically modified (GM) soybean (Roundup Ready®) powders containing CaMV35S promoter were employed as detection targets, which were amplified by loop-mediated isothermal amplification (LAMP). The CRISPR/Cas12a system directly mixed with amplified products at 37 °C for 5 min and detection results could be clearly identified by the naked eye under UV light (254 nm). A designed Reaction Vessel was employed to make operation easier and could effectively prevent contamination at the source. The CRISPR/Cas12a detection system was optimized in our study and the concentration of magnesium ions was proved to be important for the work of CRISPR/Cas12a system. The optimized concentration range of magnesium ions was between 10 mM and 12 mM. Besides, the activated Bst DNA polymerase also had little effects on CRISPR/Cas12a system. The developed method could significantly distinguish the specific and non-specific amplification. And as low as 0.05% transgenic contents in soybean powders could be detected. It would have the potential to be complementary to instrument-based ultrahigh sensitive method and provide a new solution for on-site rapid detection.
