The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Michel C Nussenzweig - One of the best experts on this subject based on the ideXlab platform.
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role of antigen Receptor Affinity in t cell independent antibody responses in vivo
Nature Immunology, 2002Co-Authors: Tienan Yang Shih, Mario Roederer, Michel C NussenzweigAbstract:To examine how B cell Receptor Affinity affects clonal selection in thymus-independent type 2 (TI-2) immune responses, we produced mice with antibodies that showed a 40-fold difference in Affinity for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The difference in the responses of high- and low-Affinity B cells to NP-Ficoll was only twofold. However, in competition experiments only the high-Affinity B cells responded to antigen. CD19 deficiency increased the Affinity threshold of TI-2 responses, whereas Lyn deficiency enhanced clonal expansion but abrogated B cell terminal differentiation. Thus, in TI-2 immune responses, large differences in Affinity produce only small differences in the intrinsic ability of B cells to respond to antigen, and selection for high-Affinity clones is due to clonal competition during the earliest stages of the response.
John W Kappler - One of the best experts on this subject based on the ideXlab platform.
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an inverse relationship between t cell Receptor Affinity and antigen dose during cd4 t cell responses in vivo and in vitro
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Kent T Teague, Frances Crawford, Philippa Marrack, Ross M Kedl, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell Receptor Affinity, we saw little correlation of immunizing peptide dose to T cell Receptor Affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent Receptor Affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.
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An inverse relationship between T cell Receptor Affinity and antigen dose during CD4(+) T cell responses in vivo and in vitro.
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Frances Crawford, T. Kent Teague, Philippa Marrack, Ross M Kedl, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell Receptor Affinity, we saw little correlation of immunizing peptide dose to T cell Receptor Affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent Receptor Affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.
Bryan L Roth - One of the best experts on this subject based on the ideXlab platform.
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Structural determinants for high 5-HT(2A) Receptor Affinity of spiro[9,10-dihydroanthracene]-9,3(')-pyrrolidine (SpAMDA).
Bioorganic & Medicinal Chemistry Letters, 2004Co-Authors: Srinivas Peddi, Richard A Glennon, Bryan L Roth, Richard B WestkaemperAbstract:Abstract The synthesis and 5-HT2A Receptor affinities of ring altered derivatives of spiro[9,10-dihydroanthracene]-9,3′-pyrrolidine (4), a structurally unique tetracyclic 5-HT2A Receptor antagonist, are described. The characteristics of the parent compound prove to be necessary for optimal 5-HT2A Receptor Affinity. However, expansion of the size of the pyrrolidine and central rings produce compounds with reasonably high 5-HT2A Receptor affinities. In addition, the parent compound is shown to have high 5-HT2 Receptor selectivity.
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h1 histamine Receptor Affinity predicts short term weight gain for typical and atypical antipsychotic drugs
Neuropsychopharmacology, 2003Co-Authors: Wesley K Kroeze, Sandra J Hufeisen, Beth Popadak, Sean Michael Renock, Seanna Steinberg, Paul Ernsberger, Karu Jayathilake, Herbert Y. Meltzer, Bryan L RothAbstract:H1-Histamine Receptor Affinity Predicts Short-Term Weight Gain for Typical and Atypical Antipsychotic Drugs
Tienan Yang Shih - One of the best experts on this subject based on the ideXlab platform.
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role of antigen Receptor Affinity in t cell independent antibody responses in vivo
Nature Immunology, 2002Co-Authors: Tienan Yang Shih, Mario Roederer, Michel C NussenzweigAbstract:To examine how B cell Receptor Affinity affects clonal selection in thymus-independent type 2 (TI-2) immune responses, we produced mice with antibodies that showed a 40-fold difference in Affinity for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The difference in the responses of high- and low-Affinity B cells to NP-Ficoll was only twofold. However, in competition experiments only the high-Affinity B cells responded to antigen. CD19 deficiency increased the Affinity threshold of TI-2 responses, whereas Lyn deficiency enhanced clonal expansion but abrogated B cell terminal differentiation. Thus, in TI-2 immune responses, large differences in Affinity produce only small differences in the intrinsic ability of B cells to respond to antigen, and selection for high-Affinity clones is due to clonal competition during the earliest stages of the response.
William Rees - One of the best experts on this subject based on the ideXlab platform.
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an inverse relationship between t cell Receptor Affinity and antigen dose during cd4 t cell responses in vivo and in vitro
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Kent T Teague, Frances Crawford, Philippa Marrack, Ross M Kedl, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell Receptor Affinity, we saw little correlation of immunizing peptide dose to T cell Receptor Affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent Receptor Affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.
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An inverse relationship between T cell Receptor Affinity and antigen dose during CD4(+) T cell responses in vivo and in vitro.
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Frances Crawford, T. Kent Teague, Philippa Marrack, Ross M Kedl, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell Receptor Affinity, we saw little correlation of immunizing peptide dose to T cell Receptor Affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent Receptor Affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.
