The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Per Artursson - One of the best experts on this subject based on the ideXlab platform.
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mechanism of absorption enhancement in humans after Rectal Administration of ampicillin in suppositories containing sodium caprate
Pharmaceutical Research, 1997Co-Authors: Tuulikki Lindmark, Johan D Soderholm, Gunnar Olaison, G Alvan, Goran Ocklind, Per ArturssonAbstract:Purpose. The medium chain fatty acid sodium caprate (C10) is approved as an absorption enhancer but its mechanism of action has not been studied in humans. The aim of this study was to investigate the mechanism of action of C10 in human subjects after Rectal Administration.
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relative contribution of phosphatidylcholine and monoglyceride to absorption enhancement of low molecular weight heparin fragmin by a new lipid based drug delivery system in monolayers of human intestinal epithelial caco 2 cells and after Rectal admi
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The relative contribution of the constituents of a novel drug delivery system based on a lipid matrix to its absorption-enhancing properties was studied in monolayers of human intestinal epithelial Caco-2 cells and after Rectal Administration to New Zealand White rabbits. Chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol were chosen as lipid matrix (PC:MG). Five different compositions of PC:MG ranging from pure MG through 1:3, 1:1 and 3:1 (w/w) mixtures to pure PC were investigated for their effects on cell toxicity, epithelial permeability and transport of low molecular weight heparin (Fragmin) and mannitol in Caco-2 monolayers. The results indicate that MG enhances and PC inhibits all of these effects in the monolayers. Similar results were obtained after Rectal Administration to New Zealand White rabbits, suggesting that the effects of PC:MG can be controlled in vivo by varying the relative proportions of MG and PC in the mixtures.
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effects of a new lipid based drug delivery system on the absorption of low molecular weight heparin fragmin through monolayers of human intestinal epithelial caco 2 cells and after Rectal Administration to rabbits
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The effects of a novel drug delivery system, consisting of a lipid matrix and a drug, on the permeability, morphology and drug transport across monolayers of human intestinal epithelial cells were investigated. A 1:3 mixture (w/w) of chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol was chosen as lipid matrix (PC:MG) and mannitol (mol. wt. 182) and Fragmin (low molecular weight heparin; mean mol. wt. 5000) were chosen as hydrophilic model drugs. PC:MG had an immediate and dose-dependent effect on the permeability of the cell monolayers, and on epithelial morphology (as seen under transmission electron microscopy). PC:MG (4–10 mM) dose-dependently enhanced the transport rate of mannitol and Fragmin, causing an approximately 10-fold increase in the transport rate of Fragmin at concentrations of 6–8 mM. The increase was independent of the dose of Fragmin and was reversible in concentrations up to 6 mM. At higher doses, clear effects on the apical cell membranes were observed although the tight junctions remained intact. PC:MG also enhanced Fragmin absorption in vivo after Rectal Administration to New Zealand White rabbits. Co-Administration of Fragmin with PC:MG (7 mM) resulted in an increase in the relative bioavailability (compared with s.c. Administration) from
L Lohikangas - One of the best experts on this subject based on the ideXlab platform.
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relative contribution of phosphatidylcholine and monoglyceride to absorption enhancement of low molecular weight heparin fragmin by a new lipid based drug delivery system in monolayers of human intestinal epithelial caco 2 cells and after Rectal admi
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The relative contribution of the constituents of a novel drug delivery system based on a lipid matrix to its absorption-enhancing properties was studied in monolayers of human intestinal epithelial Caco-2 cells and after Rectal Administration to New Zealand White rabbits. Chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol were chosen as lipid matrix (PC:MG). Five different compositions of PC:MG ranging from pure MG through 1:3, 1:1 and 3:1 (w/w) mixtures to pure PC were investigated for their effects on cell toxicity, epithelial permeability and transport of low molecular weight heparin (Fragmin) and mannitol in Caco-2 monolayers. The results indicate that MG enhances and PC inhibits all of these effects in the monolayers. Similar results were obtained after Rectal Administration to New Zealand White rabbits, suggesting that the effects of PC:MG can be controlled in vivo by varying the relative proportions of MG and PC in the mixtures.
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effects of a new lipid based drug delivery system on the absorption of low molecular weight heparin fragmin through monolayers of human intestinal epithelial caco 2 cells and after Rectal Administration to rabbits
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The effects of a novel drug delivery system, consisting of a lipid matrix and a drug, on the permeability, morphology and drug transport across monolayers of human intestinal epithelial cells were investigated. A 1:3 mixture (w/w) of chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol was chosen as lipid matrix (PC:MG) and mannitol (mol. wt. 182) and Fragmin (low molecular weight heparin; mean mol. wt. 5000) were chosen as hydrophilic model drugs. PC:MG had an immediate and dose-dependent effect on the permeability of the cell monolayers, and on epithelial morphology (as seen under transmission electron microscopy). PC:MG (4–10 mM) dose-dependently enhanced the transport rate of mannitol and Fragmin, causing an approximately 10-fold increase in the transport rate of Fragmin at concentrations of 6–8 mM. The increase was independent of the dose of Fragmin and was reversible in concentrations up to 6 mM. At higher doses, clear effects on the apical cell membranes were observed although the tight junctions remained intact. PC:MG also enhanced Fragmin absorption in vivo after Rectal Administration to New Zealand White rabbits. Co-Administration of Fragmin with PC:MG (7 mM) resulted in an increase in the relative bioavailability (compared with s.c. Administration) from
M Einarsson - One of the best experts on this subject based on the ideXlab platform.
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relative contribution of phosphatidylcholine and monoglyceride to absorption enhancement of low molecular weight heparin fragmin by a new lipid based drug delivery system in monolayers of human intestinal epithelial caco 2 cells and after Rectal admi
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The relative contribution of the constituents of a novel drug delivery system based on a lipid matrix to its absorption-enhancing properties was studied in monolayers of human intestinal epithelial Caco-2 cells and after Rectal Administration to New Zealand White rabbits. Chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol were chosen as lipid matrix (PC:MG). Five different compositions of PC:MG ranging from pure MG through 1:3, 1:1 and 3:1 (w/w) mixtures to pure PC were investigated for their effects on cell toxicity, epithelial permeability and transport of low molecular weight heparin (Fragmin) and mannitol in Caco-2 monolayers. The results indicate that MG enhances and PC inhibits all of these effects in the monolayers. Similar results were obtained after Rectal Administration to New Zealand White rabbits, suggesting that the effects of PC:MG can be controlled in vivo by varying the relative proportions of MG and PC in the mixtures.
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effects of a new lipid based drug delivery system on the absorption of low molecular weight heparin fragmin through monolayers of human intestinal epithelial caco 2 cells and after Rectal Administration to rabbits
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The effects of a novel drug delivery system, consisting of a lipid matrix and a drug, on the permeability, morphology and drug transport across monolayers of human intestinal epithelial cells were investigated. A 1:3 mixture (w/w) of chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol was chosen as lipid matrix (PC:MG) and mannitol (mol. wt. 182) and Fragmin (low molecular weight heparin; mean mol. wt. 5000) were chosen as hydrophilic model drugs. PC:MG had an immediate and dose-dependent effect on the permeability of the cell monolayers, and on epithelial morphology (as seen under transmission electron microscopy). PC:MG (4–10 mM) dose-dependently enhanced the transport rate of mannitol and Fragmin, causing an approximately 10-fold increase in the transport rate of Fragmin at concentrations of 6–8 mM. The increase was independent of the dose of Fragmin and was reversible in concentrations up to 6 mM. At higher doses, clear effects on the apical cell membranes were observed although the tight junctions remained intact. PC:MG also enhanced Fragmin absorption in vivo after Rectal Administration to New Zealand White rabbits. Co-Administration of Fragmin with PC:MG (7 mM) resulted in an increase in the relative bioavailability (compared with s.c. Administration) from
M Wilen - One of the best experts on this subject based on the ideXlab platform.
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relative contribution of phosphatidylcholine and monoglyceride to absorption enhancement of low molecular weight heparin fragmin by a new lipid based drug delivery system in monolayers of human intestinal epithelial caco 2 cells and after Rectal admi
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The relative contribution of the constituents of a novel drug delivery system based on a lipid matrix to its absorption-enhancing properties was studied in monolayers of human intestinal epithelial Caco-2 cells and after Rectal Administration to New Zealand White rabbits. Chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol were chosen as lipid matrix (PC:MG). Five different compositions of PC:MG ranging from pure MG through 1:3, 1:1 and 3:1 (w/w) mixtures to pure PC were investigated for their effects on cell toxicity, epithelial permeability and transport of low molecular weight heparin (Fragmin) and mannitol in Caco-2 monolayers. The results indicate that MG enhances and PC inhibits all of these effects in the monolayers. Similar results were obtained after Rectal Administration to New Zealand White rabbits, suggesting that the effects of PC:MG can be controlled in vivo by varying the relative proportions of MG and PC in the mixtures.
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effects of a new lipid based drug delivery system on the absorption of low molecular weight heparin fragmin through monolayers of human intestinal epithelial caco 2 cells and after Rectal Administration to rabbits
European Journal of Pharmaceutical Sciences, 1994Co-Authors: L Lohikangas, M Wilen, M Einarsson, Per ArturssonAbstract:Abstract The effects of a novel drug delivery system, consisting of a lipid matrix and a drug, on the permeability, morphology and drug transport across monolayers of human intestinal epithelial cells were investigated. A 1:3 mixture (w/w) of chromatographically purified soybean phosphatidylcholine and medium chain monoacylglycerol was chosen as lipid matrix (PC:MG) and mannitol (mol. wt. 182) and Fragmin (low molecular weight heparin; mean mol. wt. 5000) were chosen as hydrophilic model drugs. PC:MG had an immediate and dose-dependent effect on the permeability of the cell monolayers, and on epithelial morphology (as seen under transmission electron microscopy). PC:MG (4–10 mM) dose-dependently enhanced the transport rate of mannitol and Fragmin, causing an approximately 10-fold increase in the transport rate of Fragmin at concentrations of 6–8 mM. The increase was independent of the dose of Fragmin and was reversible in concentrations up to 6 mM. At higher doses, clear effects on the apical cell membranes were observed although the tight junctions remained intact. PC:MG also enhanced Fragmin absorption in vivo after Rectal Administration to New Zealand White rabbits. Co-Administration of Fragmin with PC:MG (7 mM) resulted in an increase in the relative bioavailability (compared with s.c. Administration) from
T A Mcallister - One of the best experts on this subject based on the ideXlab platform.
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oral and Rectal Administration of bacteriophages for control of escherichia coli o157 h7 in feedlot cattle
Journal of Food Protection, 2009Co-Authors: Erica A Rozema, Tyler P Stephens, S J Bach, E K Okine, Roger P Johnson, Kim Stanford, T A McallisterAbstract:This study compared oral and Rectal Administration of O157-specific bacteriophages for mitigating the fecal shedding of Escherichia coli O157 by experimentally inoculated steers. Fecal shedding of nalidixic acid-resistant (Nal(R)) E. coli O157:H7 was monitored over 83 days after oral (ORL; 3.3 x 10(11) PFU), Rectal (REC; 1.5 x 10(11) PFU), both oral and Rectal (O+R; 4.8 x 10(11) PFU), or no (CON; control) treatment with a four-strain O157-specific bacteriophage cocktail in multiple doses. Bacteriophages were enumerated by plaque assay, and NalR E. coli O157:H7 by direct plating on sorbitol MacConkey agar supplemented with cefixime, potassium tellurite, and nalidixic acid. Orally treated steers produced the fewest Nal(R) E. coli O157:H7 culture-positive samples (P 0.05) in the number of E. coli O157-positive samples among treatments. Bacteriophage was isolated from CON steers, indicating that these steers acquired the bacteriophage from the environment and shed the phage at a level similar to that of REC steers (P = 0.39). Continuous bacteriophage therapy may be an efficacious method for mitigating shedding of E. coli O157:H7 in cattle, providing that the host bacterium does not develop resistance. This therapy may be especially advantageous if nontreated cattle can acquire this biocontrol agent from the feedlot environment.