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Prem Puri - One of the best experts on this subject based on the ideXlab platform.
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ASSOCIATION OF TRANSFORMING GROWTH FACTOR-β1 GENE POLYMORPHISM WITH Reflux Nephropathy
The Journal of Urology, 2005Co-Authors: Valeria Solari, Dwight Owen, Prem PuriAbstract:ABSTRACTPurpose:: Reflux Nephropathy (RN) is recognized as a major cause of end stage renal failure in children and young adults. Transforming growth factor-β1 (TGF-β1) is a potent proinflammatory and fibrogenetic cytokine known to have a key role in the regulation of renal tissue fibrosis. We investigate genotype frequencies for polymorphisms of the TGF-β1 gene at position −509, codon 10 and 25, and examine circulating levels of TGF-β1 in patients with Reflux Nephropathy.Materials and Methods:: Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed in 123 patients with severe to moderate Reflux Nephropathy and 58 controls for the position −509, the coding region at position 10 and 25 of the TGF-β1 gene polymorphisms by polymerase chain reaction, gel analysis and appropriate restriction digest. TGF-β1 serum levels were measured with standard ELISA technique.Results:: The genotype distribution of −509-CT was significantly increased in the RN group compar...
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Association of transforming growth factor-beta1 gene polymorphism with Reflux Nephropathy.
The Journal of urology, 2005Co-Authors: Valeria Solari, Dwight Owen, Prem PuriAbstract:Reflux Nephropathy (RN) is recognized as a major cause of end stage renal failure in children and young adults. Transforming growth factor-beta1 (TGF-beta1) is a potent proinflammatory and fibrogenetic cytokine known to have a key role in the regulation of renal tissue fibrosis. We investigate genotype frequencies for polymorphisms of the TGF-beta1 gene at position -509, codon 10 and 25, and examine circulating levels of TGF-beta1 in patients with Reflux Nephropathy. Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed in 123 patients with severe to moderate Reflux Nephropathy and 58 controls for the position -509, the coding region at position 10 and 25 of the TGF-beta1 gene polymorphisms by polymerase chain reaction, gel analysis and appropriate restriction digest. TGF-beta1 serum levels were measured with standard ELISA technique. The genotype distribution of -509-CT was significantly increased in the RN group compared to controls, (82% vs 37%). Similarly, there was a significant increase in the CC Lue(10)-->Pro (codon 10) genotype (77% vs 27.5%, p <0.05), while TC Lue(10)-->Pro was significantly lower (7.3% vs 43%, p <0.05) in patients compared to controls. There was no significant difference in the Arg(25)-->Pro (codon 25) TGF-beta1 genotypes distribution between patients and controls. There were no statistically significant differences in the serum levels of TGF-beta1 in children with RN (4.2 +/- 0.3 mIU/ml) compared to controls (3.9 +/- 0.4 mIU/ml) (p >0.05). Patients with TGF-beta1 -509 and Lue(10)-->Pro gene polymorphisms may be at higher risk for Reflux Nephropathy.
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TUMOR NECROSIS FACTOR-α GENE POLYMORPHISM IN Reflux Nephropathy
The Journal of Urology, 2004Co-Authors: Valeria Solari, Sean Ennis, Salvatore Cascio, Prem PuriAbstract:ABSTRACTPurpose:: Interstitial scarring contributes to the progression of renal failure in Reflux Nephropathy. The proinflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in the disease susceptibility and pathogenesis of several inflammatory diseases promoting interstitial infiltration of inflammatory cells. We evaluate the frequency of TNF-α gene polymorphism in patients with Reflux Nephropathy.Material and Methods:: Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed on 104 patients with Reflux Nephropathy and 30 controls for the TNF-α gene polymorphisms using polymerase chain reaction and restriction digest. This polymorphism involved a guanidine to adenosine transition at position −308 and was designated as TNF1 (−308G) and TNF2 (−308A).Results:: The allele frequencies of TNF1 and TNF2 were 18.8% and 81.2% in patients with Reflux Nephropathy and 76.7% and 23.3% in controls, respectively. The genotype distribution of TNF-α-AA ...
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Insulin-like growth factor-1 expression in Reflux Nephropathy.
Pediatric surgery international, 2004Co-Authors: Boris Chertin, Amicur Farkas, Prem PuriAbstract:Background Reflux Nephropathy (RN) is recognised as a major cause of end-stage renal failure in children and young adults. Insulin-like growth factor-1 (IGF-1), a peptide growth factor produced by collecting ducts, and its receptor, insulin-like growth factor-1 receptor (IGF-1R), are present in the glomeruli and basolateral membrane of renal proximal tubular cells. Exogenous IGF-1 has been shown to enhance proliferation and reduce apoptosis of tubular cells following renal injury.
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Tumor necrosis factor-alpha gene polymorphism in Reflux Nephropathy.
The Journal of urology, 2004Co-Authors: Valeria Solari, Sean Ennis, Salvatore Cascio, Prem PuriAbstract:Interstitial scarring contributes to the progression of renal failure in Reflux Nephropathy. The proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) has been implicated in the disease susceptibility and pathogenesis of several inflammatory diseases promoting interstitial infiltration of inflammatory cells. We evaluate the frequency of TNF-alpha gene polymorphism in patients with Reflux Nephropathy. Renal scarring was evaluated with technetium dimercapto-succinic acid renal scan. Genotyping was performed on 104 patients with Reflux Nephropathy and 30 controls for the TNF-alpha gene polymorphisms using polymerase chain reaction and restriction digest. This polymorphism involved a guanidine to adenosine transition at position -308 and was designated as TNF1 (-308G) and TNF2 (-308A). The allele frequencies of TNF1 and TNF2 were 18.8% and 81.2% in patients with Reflux Nephropathy and 76.7% and 23.3% in controls, respectively. The genotype distribution of TNF-alpha-AA was significantly higher (66.4% vs 10%, p <0.05), while the TNF-alpha-GG was lower (13.4% vs 60%, p <0.05) in patients with Reflux Nephropathy compared to controls. This study demonstrates for the first time the association of the cytokine TNF-alpha gene polymorphism in patients with Reflux Nephropathy. Our data suggest that patients with vesicoureteral Reflux and TNF-alpha AA genotype may have increased susceptibility to Reflux Nephropathy.
Valeria Solari - One of the best experts on this subject based on the ideXlab platform.
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ASSOCIATION OF TRANSFORMING GROWTH FACTOR-β1 GENE POLYMORPHISM WITH Reflux Nephropathy
The Journal of Urology, 2005Co-Authors: Valeria Solari, Dwight Owen, Prem PuriAbstract:ABSTRACTPurpose:: Reflux Nephropathy (RN) is recognized as a major cause of end stage renal failure in children and young adults. Transforming growth factor-β1 (TGF-β1) is a potent proinflammatory and fibrogenetic cytokine known to have a key role in the regulation of renal tissue fibrosis. We investigate genotype frequencies for polymorphisms of the TGF-β1 gene at position −509, codon 10 and 25, and examine circulating levels of TGF-β1 in patients with Reflux Nephropathy.Materials and Methods:: Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed in 123 patients with severe to moderate Reflux Nephropathy and 58 controls for the position −509, the coding region at position 10 and 25 of the TGF-β1 gene polymorphisms by polymerase chain reaction, gel analysis and appropriate restriction digest. TGF-β1 serum levels were measured with standard ELISA technique.Results:: The genotype distribution of −509-CT was significantly increased in the RN group compar...
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Association of transforming growth factor-beta1 gene polymorphism with Reflux Nephropathy.
The Journal of urology, 2005Co-Authors: Valeria Solari, Dwight Owen, Prem PuriAbstract:Reflux Nephropathy (RN) is recognized as a major cause of end stage renal failure in children and young adults. Transforming growth factor-beta1 (TGF-beta1) is a potent proinflammatory and fibrogenetic cytokine known to have a key role in the regulation of renal tissue fibrosis. We investigate genotype frequencies for polymorphisms of the TGF-beta1 gene at position -509, codon 10 and 25, and examine circulating levels of TGF-beta1 in patients with Reflux Nephropathy. Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed in 123 patients with severe to moderate Reflux Nephropathy and 58 controls for the position -509, the coding region at position 10 and 25 of the TGF-beta1 gene polymorphisms by polymerase chain reaction, gel analysis and appropriate restriction digest. TGF-beta1 serum levels were measured with standard ELISA technique. The genotype distribution of -509-CT was significantly increased in the RN group compared to controls, (82% vs 37%). Similarly, there was a significant increase in the CC Lue(10)-->Pro (codon 10) genotype (77% vs 27.5%, p <0.05), while TC Lue(10)-->Pro was significantly lower (7.3% vs 43%, p <0.05) in patients compared to controls. There was no significant difference in the Arg(25)-->Pro (codon 25) TGF-beta1 genotypes distribution between patients and controls. There were no statistically significant differences in the serum levels of TGF-beta1 in children with RN (4.2 +/- 0.3 mIU/ml) compared to controls (3.9 +/- 0.4 mIU/ml) (p >0.05). Patients with TGF-beta1 -509 and Lue(10)-->Pro gene polymorphisms may be at higher risk for Reflux Nephropathy.
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TUMOR NECROSIS FACTOR-α GENE POLYMORPHISM IN Reflux Nephropathy
The Journal of Urology, 2004Co-Authors: Valeria Solari, Sean Ennis, Salvatore Cascio, Prem PuriAbstract:ABSTRACTPurpose:: Interstitial scarring contributes to the progression of renal failure in Reflux Nephropathy. The proinflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in the disease susceptibility and pathogenesis of several inflammatory diseases promoting interstitial infiltration of inflammatory cells. We evaluate the frequency of TNF-α gene polymorphism in patients with Reflux Nephropathy.Material and Methods:: Renal scaring was evaluated with 99technetium dimercapto-succinic acid renal scan. Genotyping was performed on 104 patients with Reflux Nephropathy and 30 controls for the TNF-α gene polymorphisms using polymerase chain reaction and restriction digest. This polymorphism involved a guanidine to adenosine transition at position −308 and was designated as TNF1 (−308G) and TNF2 (−308A).Results:: The allele frequencies of TNF1 and TNF2 were 18.8% and 81.2% in patients with Reflux Nephropathy and 76.7% and 23.3% in controls, respectively. The genotype distribution of TNF-α-AA ...
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Tumor necrosis factor-alpha gene polymorphism in Reflux Nephropathy.
The Journal of urology, 2004Co-Authors: Valeria Solari, Sean Ennis, Salvatore Cascio, Prem PuriAbstract:Interstitial scarring contributes to the progression of renal failure in Reflux Nephropathy. The proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) has been implicated in the disease susceptibility and pathogenesis of several inflammatory diseases promoting interstitial infiltration of inflammatory cells. We evaluate the frequency of TNF-alpha gene polymorphism in patients with Reflux Nephropathy. Renal scarring was evaluated with technetium dimercapto-succinic acid renal scan. Genotyping was performed on 104 patients with Reflux Nephropathy and 30 controls for the TNF-alpha gene polymorphisms using polymerase chain reaction and restriction digest. This polymorphism involved a guanidine to adenosine transition at position -308 and was designated as TNF1 (-308G) and TNF2 (-308A). The allele frequencies of TNF1 and TNF2 were 18.8% and 81.2% in patients with Reflux Nephropathy and 76.7% and 23.3% in controls, respectively. The genotype distribution of TNF-alpha-AA was significantly higher (66.4% vs 10%, p <0.05), while the TNF-alpha-GG was lower (13.4% vs 60%, p <0.05) in patients with Reflux Nephropathy compared to controls. This study demonstrates for the first time the association of the cytokine TNF-alpha gene polymorphism in patients with Reflux Nephropathy. Our data suggest that patients with vesicoureteral Reflux and TNF-alpha AA genotype may have increased susceptibility to Reflux Nephropathy.
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Cyclooxygenase-2 up-regulation in Reflux Nephropathy.
The Journal of urology, 2003Co-Authors: Valeria Solari, Kei Unemoto, Boris Chertin, Salvatore Cascio, Anna Piaseczna Piotrowska, Prem PuriAbstract:Reflux Nephropathy (RN) is a major cause of end stage renal failure in children and hypertension is a frequent complication. Cyclooxygenase-2 (COX-2) is an enzyme responsible for the prostaglandin synthesis. It has been shown that COX-2 up-regulates renin production leading to renovascular hypertension. We investigate COX-2 expression in the kidneys of children with RN. Kidney specimens from 12 patients 2 to 13 years old with severe RN secondary to primary high grade vesicoureteral Reflux obtained at the time of nephrectomy and 5 controls were examined. Single labeled immunohistochemistry was performed with 2 COX-2 antibodies using light and confocal microscopy. Quantification of COX-2 was determined by Western blotting analysis. COX-2 gene expression was evaluated by real-time quantitative reverse transcription polymerase chain reaction. There was a strong COX-2 immunoreactivity in the proximal tubules and tubulointerstitial space in the RN samples compared to controls. Immunoreactive COX-2 protein expression was markedly increased in RN samples compared to controls. Real-time reverse transcription polymerase chain reaction showed a significant increase in COX-2 mRNA expression in the RN samples compared to controls (p <0.05). Over expression of COX-2 in Reflux Nephropathy suggests that COX-2 may be involved in the pathogenesis of tubulointerstitial damage associated with severe Reflux Nephropathy.
Christopher S Cooper - One of the best experts on this subject based on the ideXlab platform.
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KIDNEYS WITH Reflux Nephropathy MAINTAIN RELATIVE RENAL FUNCTION AFTER URETERAL REIMPLANTATION
The Journal of Urology, 2005Co-Authors: Kenneth G Nepple, J Christopher Austin, Charles E Hawtrey, Christopher S CooperAbstract:ABSTRACTPurpose:: In children with severe unilateral Reflux Nephropathy and diminished relative renal function (RRF) a dilemma exists between the choice of treatment with ureteral reimplantation or nephrectomy. Limited followup data are available regarding relative renal function or postoperative complications after ureteral reimplantation in kidneys with significant unilateral Reflux Nephropathy.Materials and Methods:: We retrospectively reviewed the records of 460 patients who underwent ureteral reimplantation between 1980 and 2002, and identified children with primary vesicoureteral Reflux and severe unilateral Reflux Nephropathy (RRF 30% or less on renal scintigraphy). The postoperative outcomes were assessed for relative renal function and complications including hypertension, pyelonephritis or persistent Reflux.Results:: A total of 18 girls and 14 boys with a mean preoperative relative renal function of 20.1 ± 7.8% (range 2% to 30%) met the inclusion criteria. Reflux grade in the poorly functioning ...
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Kidneys with Reflux Nephropathy maintain relative renal function after ureteral reimplantation.
The Journal of urology, 2005Co-Authors: Kenneth G Nepple, J Christopher Austin, Charles E Hawtrey, Christopher S CooperAbstract:In children with severe unilateral Reflux Nephropathy and diminished relative renal function (RRF) a dilemma exists between the choice of treatment with ureteral reimplantation or nephrectomy. Limited followup data are available regarding relative renal function or postoperative complications after ureteral reimplantation in kidneys with significant unilateral Reflux Nephropathy. We retrospectively reviewed the records of 460 patients who underwent ureteral reimplantation between 1980 and 2002, and identified children with primary vesicoureteral Reflux and severe unilateral Reflux Nephropathy (RRF 30% or less on renal scintigraphy). The postoperative outcomes were assessed for relative renal function and complications including hypertension, pyelonephritis or persistent Reflux. A total of 18 girls and 14 boys with a mean preoperative relative renal function of 20.1 +/- 7.8% (range 2% to 30%) met the inclusion criteria. Reflux grade in the poorly functioning kidney was II in 4 children (13%), III in 14 (44%), IV in 11 (34%) and V in 3 (9%). Reflux was unilateral in 15 children (47%) and bilateral in 17 (53%). Mean followup from surgery was 3.7 years (range 0.3 to 12.9). In 28 children with both preoperative and postoperative renal scans, mean preoperative RRF was 20.3 +/- 7.4% and mean postoperative RRF was 20.5 +/- 8.6% with a mean time between renal scans of 2.3 years. No statistically significant change was noted from preoperative to postoperative relative renal function with a mean change of +0.2 +/- 3.7% (range -6.5% to +10%, p=0.82). Postoperative complications occurred in 7 of the 32 children (22%), including hypertension (1), pyelonephritis (3) and persistent Reflux (4). Pyelonephritis occurred in 1 child with persistent Reflux. No statistically significant difference existed in mean preoperative relative renal function between those with and without complications (24.6 +/- 8.9% vs 18.8 +/- 7.2%, p = 0.09). Children who underwent ureteral reimplantation in association with unilateral Reflux Nephropathy maintained stable relative renal function.
Michael J. Dillon - One of the best experts on this subject based on the ideXlab platform.
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15-year follow-up of Reflux Nephropathy by imaging.
Clinical nephrology, 1998Co-Authors: Chulananda D. A. Goonasekera, I. Gordon, Michael J. DillonAbstract:Reflux Nephropathy i.e. renal scarring associated with vesico-ureteric Reflux and urinary tract infection is primarily a diagnosis based on renal imaging. It is well known to be associated with hypertension and renal failure. This has led to regular long-term follow-up of patients, clinically and radiologically. We report the findings of renal imaging in a cohort of 37 patients with Reflux Nephropathy 15 or more years after successful surgical correction of vesico-ureteric Reflux. The degree of renal scarring had been assessed and recorded at the beginning of the study utilizing a score on the original X-ray films. The scar scores of the current intravenous urography (IVU) imaging underestimate the degree of scarring in 35% of cases when compared with the previously recorded scar scores of the original IVU images suggesting a reduction in the renal scar score in some cases over the years. In the current review, concomitant renal images obtained by IVU and 99m Tc dimercapto succinic acid (DMSA) scanning were in agreement for scar scoring in only 50% of cases. The original scar score by IVU or the current scar score by either technique does not correlate with blood pressure, urine albumin excretion or glomerular filtration rate (GFR). We conclude that serial long-term two-dimensional renal imaging in children with damaged kidneys who no longer have vesico-ureteric Reflux, does not provide additional information that will alter clinical management. However, the changes in renal volume and echogenicity were not assessed in this study.
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tubular proteinuria in Reflux Nephropathy post ureteric re implantation
Pediatric Nephrology, 1996Co-Authors: Chulananda D. A. Goonasekera, Vanita Shah, Michael J. DillonAbstract:We studied urine protein excretion in 55 adults with Reflux Nephropathy (median age 26.9 years) who had had normal blood pressure, renal function and ureteric reimplantation in childhood. Urine retinol binding protein (RBP), N-acetyl-β-d-glucosaminidase (NAG), albumin, bacteriuria, systolic blood pressure, glomerular filtration rate (GFR), peripheral plasma renin activity (PRA) and the degree of renal scarring were measured in each subject; 20 had bilateral and 35 unilateral renal scarring; 5 were hypertensive and none were in renal failure. Urinary NAG and RBP excretions were significantly greater in the study group than in 34 healthy controls (median age 29.7 years). Within the study group, NAG excretion significantly correlated with PRA (P=0.02). RBP excretion correlated with PRA, systolic blood pressure and the laterality (bilateral vs. unilateral) of scarring (P<0.01). Urinary albumin excretion correlated with systolic blood pressure (P=0.03). We conclude that increase urinary protein, especially NAG and RBP excretion, occur late after ureteric re-implantation in Reflux Nephropathy independent of GFR. Its association with PRA supports the concept of segmental perfusion and filtration as an important mechanism that may explain the above findings.
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15-year follow-up of renin and blood pressure in Reflux Nephropathy
Lancet (London England), 1996Co-Authors: Chulananda D. A. Goonasekera, Vanita Shah, Michael J. Dillon, T M Barratt, Angela WadeAbstract:Abstract Summary Background Beginning in 1978 a cohort of patients with Reflux Nephropathy first seen at a London Children's hospital have had 5-yearly follow-ups. This is the fourth (15-year) report from that series. Methods Of the original 100 normotensive children with Reflux Nephropathy 78 were traced for the 15-year study in 1994. Five patients were excluded because of nephrectomy, ten for other reasons, and eight refused to take part, leaving 55. 26 were on oral contraceptives. Supine blood pressure and plasma renin activity (PRA) were measured, and daily sodium excretion was assessed on a sample of overnight urine. Findings Of the 55 patients (15 male, 40 female, median age 27 years, range 20-31), five had systolic and two had diastolic hypertension. Compared with the 10-year (1988) follow-up there was no change in blood pressure standard deviation scores (SDS) in this cohort. PRA showed an increasing dissociation from controls after 15 years of age and was significantly above that of controls by age 25. Exclusion of the patients on oral contraceptives did not significantly alter the results. The PRA values in 1988 were not individually predictive of the development of hypertension over the ensuing 5 years. Interpretation Previously, in the long-term study of Reflux Nephropathy, blood pressure SDS had progressively increased with age. By 15 years blood pressure had levelled out and the PRA, though raised, did not predict the development of hypertension. Oral contraceptive use did not significantly modify the results.
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Abnormalities of erythrocyte sodium transport in Reflux Nephropathy
Journal of human hypertension, 1996Co-Authors: Chulananda D. A. Goonasekera, H Jardim, Shah, Michael J. DillonAbstract:Hypertension is a complication of Reflux Nephropathy commonly occurring during adolescence and young adult life. We studied cellular sodium transport in an adolescent cohort with this condition as abnormal sodium transport is a feature of human hypertension. Thirty males and 52 females with Reflux Nephropathy, (median age 20.3 years) had erythrocyte ouabain sensitive sodium-potassium ATPase (Na/K ATPase) pump site number (Bmax) and red cell sodium concentration (RBC Na + ) measured in 1988. Six years later, 55 of those had red cell sodium-lithium counter transport (LCT) measured. On both occasions, their renal function and blood pressure (BP) were determined. Bmax in the study group (median 10.3 nmol/l) was significantly less than that of controls (median 11.45 nmol/l). Nine patients who were diagnosed as having hypertension during the 6 year study period appeared to have a lower Bmax compared with that of normotensives in the group. RBC Na + and LCT of the study group were not significantly different from that of controls. The Na/K ATPase activity is diminished, and sodium-lithium counter transport is unchanged in Reflux Nephropathy. Further study is needed to ascertain the link between these observations and the onset of high BP.
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Prediction of blood pressure from plasma renin activity in Reflux Nephropathy.
Archives of disease in childhood, 1991Co-Authors: H Jardim, Vanita Shah, J M Savage, T M Barratt, Michael J. DillonAbstract:As there is a 10% risk of hypertension developing in children with Reflux Nephropathy and the renin-angiotensin system has been implicated in its aetiology, a long term prospective study has been undertaken to explore the relationship between plasma renin activity (PRA) and blood pressure in such patients. In 1978, of 100 normotensive children with Reflux Nephropathy 8% were shown to have PRA above normal. Five years later of 85 subjects suitable for analysis 13% had increased PRA and it was shown that PRA and blood pressure SD scores significantly increased. The present study refers to the 10 year follow up in which 95 of the original group were traced but eight of these were unavailable for study and 28 others were excluded from analysis because of extraneous factors that might influence blood pressure or PRA. Results therefore on 59 have been analysed. PRA was above normal in 13/59 (20%) subjects, and PRA and blood pressure SD scores had further increased. The data continue to support the role of the renin-angiotensin system in the observed rise of blood pressure in Reflux Nephropathy, but individual PRA measurements do not appear so far to predict reliably the onset of hypertension in affected patients.
Seiichi Orikasa - One of the best experts on this subject based on the ideXlab platform.
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Clinical significance of urinary interleukin-6 in children with Reflux Nephropathy.
The Journal of urology, 2001Co-Authors: Jun Wang, Ryuichiro Konda, Kiyohide Sakai, Hiroshi Sato, Sadayoshi Ito, Seiichi OrikasaAbstract:Purpose: We determined urinary interleukin-6 (IL-6) in children with Reflux Nephropathy to evaluate the clinical significance of this cytokine in the progression of renal injury.Materials and Methods: Enrolled in this study were 34 boys and 32 girls in whom 99mtechnetium dimercapto-succinic acid renal scan showed renal scarring. Vesicoureteral Reflux had been corrected surgically at least 3 years before study entry. Urinary IL-6 was determined by enzyme-linked immunosorbent assay using spot urine samples. Simultaneously we measured serum creatinine, β2-microglobulin, α1-microglobulin, urinary α1-microglobulin and albumin. In addition, IL-6 expression was assessed by immunohistochemical study in the scarred kidneys of 3 boys and 1 girl who underwent nephrectomy due to severe Reflux Nephropathy with little function on renal scan.Results: Urinary IL-6 was significantly higher in children with severe bilateral renal scarring than in those with mild scarring and normal controls. Urinary IL-6 correlated signifi...
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SOLUBLE INTERLEUKIN-2 RECEPTOR IN CHILDREN WITH Reflux Nephropathy
The Journal of urology, 1998Co-Authors: Ryuichiro Konda, Kiyohide Sakai, Shozo Ota, Atsushi Takeda, Naoki Chida, Hiroshi Sato, Seiichi OrikasaAbstract:AbstractPurpose: Serum soluble interleukin-2 receptor level is a sensitive and quantitative marker of lymphocyte activation. We determined levels of serum soluble interleukin-2 receptor in children with Reflux Nephropathy to evaluate its clinical significance in the prediction for the progression of renal injuries.Materials and Methods: Serum soluble interleukin-2 receptor values were determined in 63 children with Reflux Nephropathy. The group consisted of 37 boys and 26 girls 10 to 18 years old. T cells (naive and memory), B cells and macrophages were evaluated immunohistochemically in the scarred kidneys of 4 other patients (3 boys and 1 girl 5 to 16 years old) who underwent nephrectomy due to severe Reflux Nephropathy with little function seen on99m technetium-dimercapto-succinic acid (DMSA) renal scan. Levels of serum soluble interleukin-2 receptor were measured by an enzyme-linked immunosorbent assay. We simultaneously determined serum levels of creatinine and beta 2-microglobulin, and urinary level...
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Urinary excretion of epidermal growth factor in children with Reflux Nephropathy
The Journal of urology, 1997Co-Authors: Ryuichiro Konda, Kiyohide Sakai, Shozo Ota, Atsushi Takeda, Naoki Chida, Seiichi OrikasaAbstract:AbstractPurpose: We determined urinary levels of epidermal growth factor in children with Reflux Nephropathy to evaluate the clinical significance of urinary epidermal growth factor.Materials and Methods: We studied 59 boys and 41 girls 3 to 15 years old with Reflux Nephropathy, and 64 boys and 36 girls 3 to 15 years old who were healthy. Levels of urinary epidermal growth factor were determined by sandwich enzyme immunoassay using spot urine samples. We also determined the levels of serum creatinine, urinary alpha 1-microglobulin and urinary microalbumin. Absolute values of function of the left and right kidneys were assessed by 99mtechnetium dimercapto-succinic acid (DMSA) uptake.Results: Levels of urinary epidermal growth factor gradually decreased with age in healthy children. There were low levels of urinary epidermal growth factor in 20 of the 44 patients (45%) with unilateral low DMSA uptake and 18 of the 19 (95%) with low total DMSA uptake (right and left uptakes). Urinary epidermal growth factor ...
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Obstructive and Reflux Nephropathy
Nihon rinsho. Japanese journal of clinical medicine, 1995Co-Authors: Seiichi Orikasa, Fukuzaki A, Konda RAbstract:Urinary tract obstruction and vesicoureteral Reflux have marked effects on renal function. Renal interstitial fibrosis and glomerular hypertrophy with subsequent focal segmental glomerulosclerosis like changes are a common consequence of chronic obstruction and vesicoureteral Reflux. Evidence suggests that vasoactive compounds and cytokines such as angiotensin II, nitric oxide, eicosanoids, TNF, TGF, EGF, PDGF, bFGF have a role in the hemodynamic and structural abnormalities that occur following obstruction of the urinary tract or vesicoureteral Reflux. Use of modulators for these compounds appears to be beneficial for treatment of obstructive or Reflux Nephropathy in near future.