The Experts below are selected from a list of 7698 Experts worldwide ranked by ideXlab platform
Wei Zhang - One of the best experts on this subject based on the ideXlab platform.
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increased lipid production of the marine oleaginous microalgae isochrysis zhangjiangensis chrysophyta by nitrogen supplement
Bioresource Technology, 2011Co-Authors: Dina Feng, Zhaoan Chen, Wei ZhangAbstract:Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae isochrysis zhangjiangensis were investigated. when nitrate was supplied at interval of 24 h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g l-1 were obtained. to confirm whether 1. zhangjiangensis accumulates lipid during nitrogen-Repletion, a two-stage cultivation method was applied. this algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. these results revealed that this algal strain can accumulate lipids under nitrogen-Repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. when cultured in an extremely high nitrate concentration, 9 g l-1 at 24 h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. this special characteristic of lipid accumulation makes i. zhangjiangensis an ideal candidate for producing biodiesel using n-rich wastewater. (c) 2011 elsevier ltd. all rights reserved.
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increased lipid production of the marine oleaginous microalgae isochrysis zhangjiangensis chrysophyta by nitrogen supplement
Bioresource Technology, 2011Co-Authors: Dina Feng, Zhaoan Chen, Song Xue, Wei ZhangAbstract:Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae Isochrysis zhangjiangensis were investigated. When nitrate was supplied at interval of 24h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g L(-1) were obtained. To confirm whether I. zhangjiangensis accumulates lipid during nitrogen-Repletion, a two-stage cultivation method was applied. This algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. These results revealed that this algal strain can accumulate lipids under nitrogen-Repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. When cultured in an extremely high nitrate concentration, 9 g L(-1) at 24h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. This special characteristic of lipid accumulation makes I. zhangjiangensis an ideal candidate for producing biodiesel using N-rich wastewater.
Dina Feng - One of the best experts on this subject based on the ideXlab platform.
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increased lipid production of the marine oleaginous microalgae isochrysis zhangjiangensis chrysophyta by nitrogen supplement
Bioresource Technology, 2011Co-Authors: Dina Feng, Zhaoan Chen, Wei ZhangAbstract:Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae isochrysis zhangjiangensis were investigated. when nitrate was supplied at interval of 24 h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g l-1 were obtained. to confirm whether 1. zhangjiangensis accumulates lipid during nitrogen-Repletion, a two-stage cultivation method was applied. this algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. these results revealed that this algal strain can accumulate lipids under nitrogen-Repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. when cultured in an extremely high nitrate concentration, 9 g l-1 at 24 h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. this special characteristic of lipid accumulation makes i. zhangjiangensis an ideal candidate for producing biodiesel using n-rich wastewater. (c) 2011 elsevier ltd. all rights reserved.
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increased lipid production of the marine oleaginous microalgae isochrysis zhangjiangensis chrysophyta by nitrogen supplement
Bioresource Technology, 2011Co-Authors: Dina Feng, Zhaoan Chen, Song Xue, Wei ZhangAbstract:Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae Isochrysis zhangjiangensis were investigated. When nitrate was supplied at interval of 24h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g L(-1) were obtained. To confirm whether I. zhangjiangensis accumulates lipid during nitrogen-Repletion, a two-stage cultivation method was applied. This algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. These results revealed that this algal strain can accumulate lipids under nitrogen-Repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. When cultured in an extremely high nitrate concentration, 9 g L(-1) at 24h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. This special characteristic of lipid accumulation makes I. zhangjiangensis an ideal candidate for producing biodiesel using N-rich wastewater.
Rojyar Khezri - One of the best experts on this subject based on the ideXlab platform.
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nad augmentation restores mitophagy and limits accelerated aging in werner syndrome
Nature Communications, 2019Co-Authors: Evandro Fei Fang, Yujun Hou, Sofie Lautrup, Martin Borch Jensen, Beimeng Yang, Tanima Sengupta, Domenica Caponio, Rojyar KhezriAbstract:Metabolic dysfunction is a primary feature of Werner syndrome (WS), a human premature aging disease caused by mutations in the gene encoding the Werner (WRN) DNA helicase. WS patients exhibit severe metabolic phenotypes, but the underlying mechanisms are not understood, and whether the metabolic deficit can be targeted for therapeutic intervention has not been determined. Here we report impaired mitophagy and depletion of NAD+, a fundamental ubiquitous molecule, in WS patient samples and WS invertebrate models. WRN regulates transcription of a key NAD+ biosynthetic enzyme nicotinamide nucleotide adenylyltransferase 1 (NMNAT1). NAD+ Repletion restores NAD+ metabolic profiles and improves mitochondrial quality through DCT-1 and ULK-1-dependent mitophagy. At the organismal level, NAD+ Repletion remarkably extends lifespan and delays accelerated aging, including stem cell dysfunction, in Caenorhabditis elegans and Drosophila melanogaster models of WS. Our findings suggest that accelerated aging in WS is mediated by impaired mitochondrial function and mitophagy, and that bolstering cellular NAD+ levels counteracts WS phenotypes.
Giuditta Perozzi - One of the best experts on this subject based on the ideXlab platform.
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proteomic analysis of zn depletion Repletion in the hormone secreting thyroid follicular cell line frtl 5
Nutrients, 2018Co-Authors: Barbara Guantario, Angela Capolupo, Maria Chiara Monti, Guido Leoni, Giulia Ranaldi, Alessandra Tosco, Liberato Marzullo, Chiara Murgia, Giuditta PerozziAbstract:Zinc deficiency predisposes to a wide spectrum of chronic diseases. The human Zn proteome was predicted to represent about 10% of the total human proteome, reflecting the broad array of metabolic functions in which this micronutrient is known to participate. In the thyroid, Zn was reported to regulate cellular homeostasis, with a yet elusive mechanism. The Fischer Rat Thyroid Cell Line FRTL-5 cell model, derived from a Fischer rat thyroid and displaying a follicular cell phenotype, was used to investigate a possible causal relationship between intracellular Zn levels and thyroid function. A proteomic approach was applied to compare proteins expressed in Zn deficiency, obtained by treating cells with the Zn-specific chelator N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylene-diamine (TPEN), with Zn repleted cells. Quantitative proteomic analysis of whole cell protein extracts was performed using stable isotope dimethyl labelling coupled to nano-ultra performance liquid chromatography-mass spectrometry (UPLC-MS). TPEN treatment led to almost undetectable intracellular Zn, while decreasing thyroglobulin secretion. Subsequent addition of ZnSO4 fully reversed these phenotypes. Comparative proteomic analysis of Zn depleted/repleted cells identified 108 proteins modulated by either treatment. Biological process enrichment analysis identified functions involved in calcium release and the regulation of translation as the most strongly regulated processes in Zn depleted cells.
Ulla Heinonen - One of the best experts on this subject based on the ideXlab platform.
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niacin cures systemic nad deficiency and improves muscle performance in adult onset mitochondrial myopathy
Cell Metabolism, 2020Co-Authors: Eija Pirinen, Nahid Akhtar Khan, Mari Auranen, Niina Urho, Antti Hakkarainen, Virginia Brilhante, Alberto Pessia, Juho Kuula, Ulla HeinonenAbstract:Summary NAD+ is a redox-active metabolite, the depletion of which has been proposed to promote aging and degenerative diseases in rodents. However, whether NAD+ depletion occurs in patients with degenerative disorders and whether NAD+ Repletion improves their symptoms has remained open. Here, we report systemic NAD+ deficiency in adult-onset mitochondrial myopathy patients. We administered an increasing dose of NAD+-booster niacin, a vitamin B3 form (to 750–1,000 mg/day; clinicaltrials.gov NCT03973203 ) for patients and their matched controls for 10 or 4 months, respectively. Blood NAD+ increased in all subjects, up to 8-fold, and muscle NAD+ of patients reached the level of their controls. Some patients showed anemia tendency, while muscle strength and mitochondrial biogenesis increased in all subjects. In patients, muscle metabolome shifted toward controls and liver fat decreased even 50%. Our evidence indicates that blood analysis is useful in identifying NAD+ deficiency and points niacin to be an efficient NAD+ booster for treating mitochondrial myopathy.
