The Experts below are selected from a list of 21216 Experts worldwide ranked by ideXlab platform
Xi Chen - One of the best experts on this subject based on the ideXlab platform.
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a dual signal amplification method for the dna detection based on exonuclease iii
Biosensors and Bioelectronics, 2014Co-Authors: Zhimin Cai, Yiying Chen, Chunshui Lin, Chaoyong James Yang, Yiru Wang, Xi ChenAbstract:A dual-signal amplification method based on two molecular beacons was designed for human hemochromatosis (HFE) gene detection. The two probes, P1 and P2, could resist the exonuclease III (Exo III) digestion due to the 3'-termini protrusion, and could coexist stably with Exo III. In the presence of HFE targets, P1 hybridized with a HFE target to form a duplex DNA with a recessed 3'-hydroxyl termini and then partially digested by Exo III, releasing the HFE target and a Residual Sequence (X). This X Sequence could trigger the digestion of P2 probes with 6-carboxy-fluoresceins and Black Hole Quenchers and then result in the increase of fluorescence intensity. The X Sequences were more stable than HFE targets and could cyclically trigger the P2 digestion for a long time even though the HFE targets were digested by Exo III. This method improved the sensitivity and reached 4 orders of magnitude in detection limit, and showed excellent selectivity to discriminate single base mismatched targets well.
Shulin Zhao - One of the best experts on this subject based on the ideXlab platform.
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a label free fluorescent probe based on dna templated silver nanoclusters and exonuclease iii assisted recycling amplification detection of nucleic acid
Analytica Chimica Acta, 2015Co-Authors: Wen Yang, Jianniao Tian, Lijun Wang, Yanchun Zhao, Shulin ZhaoAbstract:A number of specific nucleic acids are closely related with many serious diseases, in the current research, a platform taking advantage of exonuclease III (Exo III) to realize double recycling amplification and label-free fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) for detecting of nucleic acid had been developed. In this method, a molecular beacon (MB) with 3'-protruding termini and a single-stranded cytosine-rich (C-rich) probe were designed that coexist stably with Exo III. Once the target DNA appeared, portion of the MB could hybridize with target DNA and was digested by Exo III, which allowed the release of target DNA and a Residual Sequence. Subsequently, the Residual Sequence could trigger the Exo III to digest C-rich probe, and the DNA-AgNCs was not able to be synthesized because of the C-rich probe was destroyed; finally the fluorescent of solution was quenched. This assay enables to monitor human hemochromatosis gene (as a model) with high sensitivity, the detection limit is as low as 120 pM compared with other fluorescence DNA-AgNCs methods, this assay also exhibits superior specificity even against single base mismatch. The strategy is applied to detect human hemochromatosis gene in real human serum samples successfully.
Zhimin Cai - One of the best experts on this subject based on the ideXlab platform.
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a dual signal amplification method for the dna detection based on exonuclease iii
Biosensors and Bioelectronics, 2014Co-Authors: Zhimin Cai, Yiying Chen, Chunshui Lin, Chaoyong James Yang, Yiru Wang, Xi ChenAbstract:A dual-signal amplification method based on two molecular beacons was designed for human hemochromatosis (HFE) gene detection. The two probes, P1 and P2, could resist the exonuclease III (Exo III) digestion due to the 3'-termini protrusion, and could coexist stably with Exo III. In the presence of HFE targets, P1 hybridized with a HFE target to form a duplex DNA with a recessed 3'-hydroxyl termini and then partially digested by Exo III, releasing the HFE target and a Residual Sequence (X). This X Sequence could trigger the digestion of P2 probes with 6-carboxy-fluoresceins and Black Hole Quenchers and then result in the increase of fluorescence intensity. The X Sequences were more stable than HFE targets and could cyclically trigger the P2 digestion for a long time even though the HFE targets were digested by Exo III. This method improved the sensitivity and reached 4 orders of magnitude in detection limit, and showed excellent selectivity to discriminate single base mismatched targets well.
Wen Yang - One of the best experts on this subject based on the ideXlab platform.
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a label free fluorescent probe based on dna templated silver nanoclusters and exonuclease iii assisted recycling amplification detection of nucleic acid
Analytica Chimica Acta, 2015Co-Authors: Wen Yang, Jianniao Tian, Lijun Wang, Yanchun Zhao, Shulin ZhaoAbstract:A number of specific nucleic acids are closely related with many serious diseases, in the current research, a platform taking advantage of exonuclease III (Exo III) to realize double recycling amplification and label-free fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) for detecting of nucleic acid had been developed. In this method, a molecular beacon (MB) with 3'-protruding termini and a single-stranded cytosine-rich (C-rich) probe were designed that coexist stably with Exo III. Once the target DNA appeared, portion of the MB could hybridize with target DNA and was digested by Exo III, which allowed the release of target DNA and a Residual Sequence. Subsequently, the Residual Sequence could trigger the Exo III to digest C-rich probe, and the DNA-AgNCs was not able to be synthesized because of the C-rich probe was destroyed; finally the fluorescent of solution was quenched. This assay enables to monitor human hemochromatosis gene (as a model) with high sensitivity, the detection limit is as low as 120 pM compared with other fluorescence DNA-AgNCs methods, this assay also exhibits superior specificity even against single base mismatch. The strategy is applied to detect human hemochromatosis gene in real human serum samples successfully.
Yiying Chen - One of the best experts on this subject based on the ideXlab platform.
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a dual signal amplification method for the dna detection based on exonuclease iii
Biosensors and Bioelectronics, 2014Co-Authors: Zhimin Cai, Yiying Chen, Chunshui Lin, Chaoyong James Yang, Yiru Wang, Xi ChenAbstract:A dual-signal amplification method based on two molecular beacons was designed for human hemochromatosis (HFE) gene detection. The two probes, P1 and P2, could resist the exonuclease III (Exo III) digestion due to the 3'-termini protrusion, and could coexist stably with Exo III. In the presence of HFE targets, P1 hybridized with a HFE target to form a duplex DNA with a recessed 3'-hydroxyl termini and then partially digested by Exo III, releasing the HFE target and a Residual Sequence (X). This X Sequence could trigger the digestion of P2 probes with 6-carboxy-fluoresceins and Black Hole Quenchers and then result in the increase of fluorescence intensity. The X Sequences were more stable than HFE targets and could cyclically trigger the P2 digestion for a long time even though the HFE targets were digested by Exo III. This method improved the sensitivity and reached 4 orders of magnitude in detection limit, and showed excellent selectivity to discriminate single base mismatched targets well.
