The Experts below are selected from a list of 2034 Experts worldwide ranked by ideXlab platform

Kazuhiro Abe - One of the best experts on this subject based on the ideXlab platform.

  • Scanning electron microscopic studies of Reticular framework in the rat mesenteric lymph node.
    The Anatomical record, 1995
    Co-Authors: Tatsuo Ushiki, Osamu Ohtani, Kazuhiro Abe
    Abstract:

    Background: The Reticular framework in the lymph node has in the past been studied mainly by light microscopy of silver-impregnated specimens. The aim of the present study is to understand three-dimensionally the ultrastructure and organization of the Reticular framework better than before. Methods: The mesenteric lymph nodes of the rat were prepared either an alkali-water maceration method or a conventional method and were observed in a scanning electron microscope (SEM). Results: The SEM study of alkali-water macerated tissues visualized directly the Reticular Fiber network in the lymph node. The Reticular Fibers consisted of thin bundles of collagem fibrils. They were continuous with the collagen fibriliar sheaths of blood vessels and lymphatic sinuses as well as with the fibrous capusule, thus acting as a skeleton of the lymph node. The arrangement of the reticulum was variable, depending on individual compartments. The SEM study of conventionally treated tissues, on the other hand, clarified the shape of Reticular cells and their relationship with the Reticular Fibers. The sinus Reticular cells connected with the sinus lining cells but separated from the parenchymal Reticular cells, indicating that the former two originate from lymphatic endothelial cells. The parenchymal Reticular cells varied in shape depending on their locations but essentially shared features with fibroblasts. Conclusions: The arrangements of the Reticular Fibers in the parenchyma were closely related to the associated Reticular cells, showing the possibility that the Reticular cells maintain the shape of the Reticular framework suitable for each compartment of the lymph node. © 1995 Wiley-Liss, Inc.

Tatsuo Ushiki - One of the best experts on this subject based on the ideXlab platform.

  • Scanning electron microscopic studies of Reticular framework in the rat mesenteric lymph node.
    The Anatomical record, 1995
    Co-Authors: Tatsuo Ushiki, Osamu Ohtani, Kazuhiro Abe
    Abstract:

    Background: The Reticular framework in the lymph node has in the past been studied mainly by light microscopy of silver-impregnated specimens. The aim of the present study is to understand three-dimensionally the ultrastructure and organization of the Reticular framework better than before. Methods: The mesenteric lymph nodes of the rat were prepared either an alkali-water maceration method or a conventional method and were observed in a scanning electron microscope (SEM). Results: The SEM study of alkali-water macerated tissues visualized directly the Reticular Fiber network in the lymph node. The Reticular Fibers consisted of thin bundles of collagem fibrils. They were continuous with the collagen fibriliar sheaths of blood vessels and lymphatic sinuses as well as with the fibrous capusule, thus acting as a skeleton of the lymph node. The arrangement of the reticulum was variable, depending on individual compartments. The SEM study of conventionally treated tissues, on the other hand, clarified the shape of Reticular cells and their relationship with the Reticular Fibers. The sinus Reticular cells connected with the sinus lining cells but separated from the parenchymal Reticular cells, indicating that the former two originate from lymphatic endothelial cells. The parenchymal Reticular cells varied in shape depending on their locations but essentially shared features with fibroblasts. Conclusions: The arrangements of the Reticular Fibers in the parenchyma were closely related to the associated Reticular cells, showing the possibility that the Reticular cells maintain the shape of the Reticular framework suitable for each compartment of the lymph node. © 1995 Wiley-Liss, Inc.

Osamu Ohtani - One of the best experts on this subject based on the ideXlab platform.

  • Scanning electron microscopic studies of Reticular framework in the rat mesenteric lymph node.
    The Anatomical record, 1995
    Co-Authors: Tatsuo Ushiki, Osamu Ohtani, Kazuhiro Abe
    Abstract:

    Background: The Reticular framework in the lymph node has in the past been studied mainly by light microscopy of silver-impregnated specimens. The aim of the present study is to understand three-dimensionally the ultrastructure and organization of the Reticular framework better than before. Methods: The mesenteric lymph nodes of the rat were prepared either an alkali-water maceration method or a conventional method and were observed in a scanning electron microscope (SEM). Results: The SEM study of alkali-water macerated tissues visualized directly the Reticular Fiber network in the lymph node. The Reticular Fibers consisted of thin bundles of collagem fibrils. They were continuous with the collagen fibriliar sheaths of blood vessels and lymphatic sinuses as well as with the fibrous capusule, thus acting as a skeleton of the lymph node. The arrangement of the reticulum was variable, depending on individual compartments. The SEM study of conventionally treated tissues, on the other hand, clarified the shape of Reticular cells and their relationship with the Reticular Fibers. The sinus Reticular cells connected with the sinus lining cells but separated from the parenchymal Reticular cells, indicating that the former two originate from lymphatic endothelial cells. The parenchymal Reticular cells varied in shape depending on their locations but essentially shared features with fibroblasts. Conclusions: The arrangements of the Reticular Fibers in the parenchyma were closely related to the associated Reticular cells, showing the possibility that the Reticular cells maintain the shape of the Reticular framework suitable for each compartment of the lymph node. © 1995 Wiley-Liss, Inc.

Emmanuel Donnadieu - One of the best experts on this subject based on the ideXlab platform.

  • Positive and negative influence of the matrix architecture on antitumor immune surveillance
    Cellular and Molecular Life Sciences, 2013
    Co-Authors: Elisa Peranzoni, Ana Rivas-caicedo, Houcine Bougherara, Hélène Salmon, Emmanuel Donnadieu
    Abstract:

    The migration of T cells and access to tumor antigens is of utmost importance for the induction of protective anti-tumor immunity. Once having entered a malignant site, T cells encounter a complex environment composed of non-tumor cells along with the extracellular matrix (ECM). It is now well accepted that a deregulated ECM favors tumor progression and metastasis. Recent progress in imaging technologies has also highlighted the impact of the matrix architecture found in solid tumor on immune cells and especially T cells. In this review, we argue that the ability of T cells to mount an antitumor response is dependent on the matrix structure, more precisely on the balance between pro-migratory Reticular Fiber networks and unfavorable migration zones composed of dense and aligned ECM structures. Thus, the matrix architecture, that has long been considered to merely provide the structural framework of connective tissues, can play a key role in facilitating or suppressing the antitumor immune surveillance. A new challenge in cancer therapy will be to develop approaches aimed at altering the architecture of the tumor stroma, rendering it more permissive to antitumor T cells.

  • Positive and negative influence of the matrix architecture on antitumor immune surveillance
    Cellular and Molecular Life Sciences, 2013
    Co-Authors: Elisa Peranzoni, Ana Rivas-caicedo, Houcine Bougherara, Hélène Salmon, Emmanuel Donnadieu
    Abstract:

    The migration of T cells and access to tumor antigens is of utmost importance for the induction of protective anti-tumor immunity. Once having entered a malignant site, T cells encounter a complex environment composed of non-tumor cells along with the extracellular matrix (ECM). It is now well accepted that a deregulated ECM favors tumor progression and metastasis. Recent progress in imaging technologies has also highlighted the impact of the matrix architecture found in solid tumor on immune cells and especially T cells. In this review, we argue that the ability of T cells to mount an antitumor response is dependent on the matrix structure, more precisely on the balance between pro-migratory Reticular Fiber networks and unfavorable migration zones composed of dense and aligned ECM structures. Thus, the matrix architecture, that has long been considered to merely provide the structural framework of connective tissues, can play a key role in facilitating or suppressing the antitumor immune surveillance. A new challenge in cancer therapy will be to develop approaches aimed at altering the architecture of the tumor stroma, rendering it more permissive to antitumor T cells.

Sg Ramos - One of the best experts on this subject based on the ideXlab platform.

  • Interference of dexamethasone in the pulmonary cycle of Strongyloides venezuelensis in rats
    Amer Soc Trop Med & Hygiene, 2015
    Co-Authors: Tefe-silva C, Di Souza, Mt Ueta, Em Floriano, Lh Faccioli, Sg Ramos
    Abstract:

    The aim of this study was to investigate the interference of a daily treatment of dexamethasone in the pulmonary cycle of Strongyloides venezuelensis infection in rats. Three principal effects were found: 1) increased alveolar hemorrhagic inflammation provoked by the passage of larvae into alveolar spaces; 2) significant decrease of eosinophil and mast cell migration to the axial septum of the lungs; and 3) impaired formation of the Reticular Fiber network, interfering with granuloma organization. This study showed that the use of drugs with immunomodulatory actions, such as dexamethasone, in addition to interfering with the morbidity from the pulmonary cycle of S. venezuelensis infection, may contribute to showing the mechanisms involved in its pathogenesis

  • Interference of dexamethasone in the pulmonary cycle of Strongyloides venezuelensis in rats
    EUA, 2015
    Co-Authors: Tefe-silva C, Di Souza, Mt Ueta, Em Floriano, Lh Faccioli, Sg Ramos
    Abstract:

    The aim of this study was to investigate the interference of a daily treatment of dexamethasone in the pulmonary cycle of Strongyloides venezuelensis infection in rats. Three principal effects were found: 1) increased alveolar hemorrhagic inflammation provoked by the passage of larvae into alveolar spaces; 2) significant decrease of eosinophil and mast cell migration to the axial septum of the lungs; and 3) impaired formation of the Reticular Fiber network, interfering with granuloma organization. This study showed that the use of drugs with immunomodulatory actions, such as dexamethasone, in addition to interfering with the morbidity from the pulmonary cycle of S. venezuelensis infection, may contribute to showing the mechanisms involved in its pathogenesis.o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.79457157