The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Dornburg R - One of the best experts on this subject based on the ideXlab platform.
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Reticuloendotheliosis viruses and derived vectors.
Gene therapy, 1995Co-Authors: Dornburg RAbstract:In most human gene therapy trials, the tools of gene delivery are retroviral vectors. All current vectors are derived from murine leukemia virus (MLV). Although this system is suitable for delivering a large variety of genes into different tissues, it also has its limitations and is not adequate for all potential applications of human gene therapy. Thus, attempts are underway in many laboratories to develop other gene delivery tools. Potential agents range from non-viral based gene delivery systems (eg liposomes) to other viral vectors such as those derived from adenoviruses, adeno-associated viruses, herpes simplex virus, and several other viruses. Furthermore, the development of other, non-MLV retroviral vector systems, including one derived from HIV, is in progress in several laboratories. In this article, Reticuloendotheliosis viruses and their vector systems are reviewed, and their possible use in human gene therapy is discussed.
Ziqiang Cheng - One of the best experts on this subject based on the ideXlab platform.
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Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs
Retrovirology, 2018Co-Authors: Defang Zhou, Shuhai He, Chengui Li, Venugopal Nair, Libo Huang, Xusheng Du, Jing Zhou, Ziqiang ChengAbstract:Background Co-infection with avian leukosis virus subgroup J and Reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown.
Thierry Jaffredo - One of the best experts on this subject based on the ideXlab platform.
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avian Reticuloendotheliosis virus strain a and spleen necrosis virus do not infect human cells
Journal of Virology, 2000Co-Authors: Rodolphe Gautier, An Jiang, V Rousseau, Ralph Dornburg, Thierry JaffredoAbstract:Spleen necrosis virus (SNV) and Reticuloendotheliosis virus strain A (REV-A) belong to the family of Reticuloendotheliosis viruses and are 90% sequence related. SNV-derived retroviral vectors produced by the REV-A-based D17.2G packaging cell line were shown to infect human cells (H.-M. Koo, A. M. C. Brown, Y. Ron, and J. P. Dougherty, J. Virol. 65:4769–4776, 1991), while similar vectors produced by another SNV-based packaging cell line, DSH134G, are not infectious in human cells (reviewed by R. Dornburg, Gene Ther. 2:301–310, 1995). Here we describe a careful reevaluation of the infectivity of vectors produced from the most commonly used REV-A- or SNV-based packaging cells obtained from various sources with, among them, one batch of D17.2G packaging cells obtained from the American Type Culture Collection. None of these packaging cells produced vectors able to infect human cells. Thus, contrary to previously published data, we conclude that REV-based vectors are not infectious in human cells.
Ralph Dornburg - One of the best experts on this subject based on the ideXlab platform.
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Reticuloendotheliosis viruses and derived vectors for human gene therapy.
Frontiers in bioscience : a journal and virtual library, 2003Co-Authors: Ralph DornburgAbstract:The Reticuloendotheliosis viruses (REV) spleen necrosis virus (SNV) and Reticuloendotheliosis virus strain-A (REV-A) are amphotropic retroviruses which infect a large variety of cells of avian and some mammalian species. They normally do not infect primate or rodent cells. However, they efficiently infect and integrate their genome into that of human cells when they are pseudotyped with the envelope protein of other mammalian retroviruses or the G protein of vesicular stomatitis virus (VSV) or rabies viruses (RV). Moreover, SNV-derived retroviral vectors, which display single chain antibodies or other targeting ligands on the viral surface enable cell-type-specific gene delivery into various human cells. My laboratory has developed genetically engineered REV vectors, which are capable of infecting non-dividing cells such as quiescent human T-cells, primary monocyte-derived macrophages, and mature neurons. Thus, REV-derived vectors appear to be very interesting candidates for the further development of vectors for human gene therapy. This article reviews the replication of REVs and vectors derived from REV-A and SNV for gene transfer into human cells.
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avian Reticuloendotheliosis virus strain a and spleen necrosis virus do not infect human cells
Journal of Virology, 2000Co-Authors: Rodolphe Gautier, An Jiang, V Rousseau, Ralph Dornburg, Thierry JaffredoAbstract:Spleen necrosis virus (SNV) and Reticuloendotheliosis virus strain A (REV-A) belong to the family of Reticuloendotheliosis viruses and are 90% sequence related. SNV-derived retroviral vectors produced by the REV-A-based D17.2G packaging cell line were shown to infect human cells (H.-M. Koo, A. M. C. Brown, Y. Ron, and J. P. Dougherty, J. Virol. 65:4769–4776, 1991), while similar vectors produced by another SNV-based packaging cell line, DSH134G, are not infectious in human cells (reviewed by R. Dornburg, Gene Ther. 2:301–310, 1995). Here we describe a careful reevaluation of the infectivity of vectors produced from the most commonly used REV-A- or SNV-based packaging cells obtained from various sources with, among them, one batch of D17.2G packaging cells obtained from the American Type Culture Collection. None of these packaging cells produced vectors able to infect human cells. Thus, contrary to previously published data, we conclude that REV-based vectors are not infectious in human cells.
Defang Zhou - One of the best experts on this subject based on the ideXlab platform.
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Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs
Retrovirology, 2018Co-Authors: Defang Zhou, Shuhai He, Chengui Li, Venugopal Nair, Libo Huang, Xusheng Du, Jing Zhou, Ziqiang ChengAbstract:Background Co-infection with avian leukosis virus subgroup J and Reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown.
