The Experts below are selected from a list of 29298 Experts worldwide ranked by ideXlab platform
Ying Zhao - One of the best experts on this subject based on the ideXlab platform.
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anti influenza a virus activity of Rhein through regulating oxidative stress tlr4 akt mapk and nf κb signal pathways
PLOS ONE, 2018Co-Authors: Qianwen Wang, Ying Zhao, Jiangtao Sheng, Xiaoxuan Chen, Cheng Chen, Jianping DaiAbstract:Rhein, an anthraquinone compound existing in many traditional herbal medicines, has anti-inflammatory, antioxidant, antitumor, antiviral, hepatoprotective, and nephroprotective activities, but its anti-influenza A virus (IAV) activity is ambiguous. In the present study, through plaque inhibition assay, time-of-addition assay, antioxidant assay, qRT-PCR, ELISA, and western blotting assays, we investigated the anti-IAV effect and mechanism of action of Rhein in vitro and in vivo. The results showed that Rhein could significantly inhibit IAV adsorption and replication, decrease IAV-induced oxidative stress, activations of TLR4, Akt, p38, JNK MAPK, and NF-κB pathways, and production of inflammatory cytokines and matrix metalloproteinases in vitro. Oxidant H2O2 and agonists of TLR4, Akt, p38/JNK and IKK/NF-κB could significantly antagonize the inhibitory effects of Rhein on IAV-induced cytopathic effect (CPE) and IAV replication. Through an in vivo test in mice, we also found that Rhein could significantly improve the survival rate, lung index, pulmonary cytokines, and pulmonary histopathological changes. Rhein also significantly decreased pulmonary viral load at a high dose. In conclusion, Rhein can inhibit IAV adsorption and replication, and the mechanism of action to inhibit IAV replication may be due to its ability to suppress IAV-induced oxidative stress and activations of TLR4, Akt, p38, JNK MAPK, and NF-κB signal pathways.
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liver targeting effect of vinegar baked radix bupleuri on Rhein in rats
Journal of Ethnopharmacology, 2010Co-Authors: Ruizhi Zhao, You-jun Chen, Dong Yuan, Ying ZhaoAbstract:Vinegar-baked Radix Bupleuri (VBRB) is usually used to focus other drugs effect on liver in traditional Chinese medicine (TCM). However, no sufficient scientific data are available to support this concept. In this paper, the liver targeting enhancing effect of VBRB on Rhein was investigated. 432 of rats were divided into two large groups according to the dose of Rhein, low dose group of Rhein (LDGR) and high dose group of Rhein (HDGR). In each group, the rats were further divided into four subgroups, Rhein control and Rhein co-administered with three different doses of VBRB peroral. Concentrations of Rhein and its metabolite in different tissues were determined by HPLC. Compared to the control group, VBRB significantly increased the distribution of both Rhein and its metabolite in liver and meanwhile decreased their distribution in other tissues, indicating a strong liver targeting enhancing effect. This liver targeting effect of VBRB depended on the dose of VBRB and Rhein. Low and high dose of VBRB had a more strong effect than medium dose in HDGR; high dose of Rhein was more sensitive than low dose of Rhein (P < 0.05). Rhein existed in two forms after peroral administration in vivo. It was found that the liver targeting effect of VBRB was more remarkable with the native form of Rhein compared to its derivative form. The results of this paper demonstrated that co-administration with VBRB is a simple and efficiencient method for liver targeting therapy, and the meridine guide theory of TCM was credible.
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Liver targeting effect of vinegar-baked Radix Bupleuri on Rhein in rats.
Journal of ethnopharmacology, 2010Co-Authors: Ruizhi Zhao, You-jun Chen, Dong Yuan, Shaojun Liu, Li Juan Liu, Ying ZhaoAbstract:Vinegar-baked Radix Bupleuri (VBRB) is usually used to focus other drugs effect on liver in traditional Chinese medicine (TCM). However, no sufficient scientific data are available to support this concept. In this paper, the liver targeting enhancing effect of VBRB on Rhein was investigated. 432 of rats were divided into two large groups according to the dose of Rhein, low dose group of Rhein (LDGR) and high dose group of Rhein (HDGR). In each group, the rats were further divided into four subgroups, Rhein control and Rhein co-administered with three different doses of VBRB peroral. Concentrations of Rhein and its metabolite in different tissues were determined by HPLC. Compared to the control group, VBRB significantly increased the distribution of both Rhein and its metabolite in liver and meanwhile decreased their distribution in other tissues, indicating a strong liver targeting enhancing effect. This liver targeting effect of VBRB depended on the dose of VBRB and Rhein. Low and high dose of VBRB had a more strong effect than medium dose in HDGR; high dose of Rhein was more sensitive than low dose of Rhein (P
Xijun Wang - One of the best experts on this subject based on the ideXlab platform.
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uplc ms ms performing pharmacokinetic and biodistribution studies of Rhein
IEEE Journal of Solid-state Circuits, 2012Co-Authors: Hui Sun, Quanwei Yin, Aihua Zhang, Xijun WangAbstract:Abstract Rhein, an important constituent of Radix et Rhizoma Rhei, has been used to alleviate liver and kidney damage. In this work, plasma pharmacokinetic and biodistribution characteristics of Rhein after oral administration was investigated using a rapid and sensitive ultra-performance liquid chromatography coupled to tandem high-definition mass spectrometry (UPLC-MS/MS) method. Mass spectrometry was performed on a Waters Micromass high-definition technology with an electrospray ionization source in positive ion mode. Biosamples were prepared using methanolic precipitation and the separation of Rhein was achieved on a UPLC HSS T3 column by linear gradient elution and the total run time was only 4.70 min. Data were analyzed and estimated by compartmental methods using Win-Nonlin Professional version 5.1. Mean pharmacokinetic parameters following single-dose administration of Rhein was consistent with a two-compartmental open model. It was found that Rhein was distributed and eliminated rapidly in rats and the biodistribution showed the higher levels were in liver, spleen, kidney, heart, lung and the lower level observed in the muscle, adrenal, and thyroid. It was not discovered in brain and showed that Rhein could not cross the blood-brain barrier. Our developed UPLC-MS/MS approach was capable of providing complete pharmacokinetic and biodistribution parameters for Rhein when administered orally.
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UPLC–MS/MS performing pharmacokinetic and biodistribution studies of Rhein
Journal of separation science, 2012Co-Authors: Hui Sun, Quanwei Yin, Aihua Zhang, Xijun WangAbstract:Abstract Rhein, an important constituent of Radix et Rhizoma Rhei, has been used to alleviate liver and kidney damage. In this work, plasma pharmacokinetic and biodistribution characteristics of Rhein after oral administration was investigated using a rapid and sensitive ultra-performance liquid chromatography coupled to tandem high-definition mass spectrometry (UPLC-MS/MS) method. Mass spectrometry was performed on a Waters Micromass high-definition technology with an electrospray ionization source in positive ion mode. Biosamples were prepared using methanolic precipitation and the separation of Rhein was achieved on a UPLC HSS T3 column by linear gradient elution and the total run time was only 4.70 min. Data were analyzed and estimated by compartmental methods using Win-Nonlin Professional version 5.1. Mean pharmacokinetic parameters following single-dose administration of Rhein was consistent with a two-compartmental open model. It was found that Rhein was distributed and eliminated rapidly in rats and the biodistribution showed the higher levels were in liver, spleen, kidney, heart, lung and the lower level observed in the muscle, adrenal, and thyroid. It was not discovered in brain and showed that Rhein could not cross the blood-brain barrier. Our developed UPLC-MS/MS approach was capable of providing complete pharmacokinetic and biodistribution parameters for Rhein when administered orally.
Meng Liang Lin - One of the best experts on this subject based on the ideXlab platform.
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Rhein inhibits invasion and migration of human nasopharyngeal carcinoma cells in vitro by down regulation of matrix metalloproteinases 9 and vascular endothelial growth factor
Oral Oncology, 2009Co-Authors: Jing Gung Chung, Meng Liang Lin, Chiou Ying Yang, Shihshun ChenAbstract:Summary Progression of cancer invasion is believed to be dependent on the remodeling of extracellular matrix induced by tumor cells. Rhein has been shown to inhibit the growth and proliferation of human nasopharyngeal carcinoma (NPC) cells. However, the molecular mechanism underlying Rhein-induced inhibition of cancer invasion has not been explored. Herein, we show that Rhein could inhibit the invasion and migration of NPC cells in vitro. Rhein inhibits invasion by reducing the expression of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF). Moreover, we demonstrate that the pathway involved in Rhein-inhibited invasion is presumably through the growth factor receptor bound protein 2/son of sevenless-Ras-mitogen-activated protein kinase (GRB2/SOS-Ras-MAPK) pathway, as shown by an decrease in the expression levels of GRB2, SOS-1 and Ras as well as led to suppression of the phosphorylation of extracellular signal-regulated kinase (ERK) and p38 MAPK. Further study has shown that Rhein also inhibited activation of transcription factor nuclear factor κB (NF-κB), which is known to implicate the regulation of MMP-9 and VEGF gene expression in cancer invasion. Our findings suggest that Rhein inhibits the invasion of NPC cells may be mediated in part through the suppression of MMP-9 and VEGF expression via the modulation of NF-κB signaling pathway.
Hui Sun - One of the best experts on this subject based on the ideXlab platform.
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uplc ms ms performing pharmacokinetic and biodistribution studies of Rhein
IEEE Journal of Solid-state Circuits, 2012Co-Authors: Hui Sun, Quanwei Yin, Aihua Zhang, Xijun WangAbstract:Abstract Rhein, an important constituent of Radix et Rhizoma Rhei, has been used to alleviate liver and kidney damage. In this work, plasma pharmacokinetic and biodistribution characteristics of Rhein after oral administration was investigated using a rapid and sensitive ultra-performance liquid chromatography coupled to tandem high-definition mass spectrometry (UPLC-MS/MS) method. Mass spectrometry was performed on a Waters Micromass high-definition technology with an electrospray ionization source in positive ion mode. Biosamples were prepared using methanolic precipitation and the separation of Rhein was achieved on a UPLC HSS T3 column by linear gradient elution and the total run time was only 4.70 min. Data were analyzed and estimated by compartmental methods using Win-Nonlin Professional version 5.1. Mean pharmacokinetic parameters following single-dose administration of Rhein was consistent with a two-compartmental open model. It was found that Rhein was distributed and eliminated rapidly in rats and the biodistribution showed the higher levels were in liver, spleen, kidney, heart, lung and the lower level observed in the muscle, adrenal, and thyroid. It was not discovered in brain and showed that Rhein could not cross the blood-brain barrier. Our developed UPLC-MS/MS approach was capable of providing complete pharmacokinetic and biodistribution parameters for Rhein when administered orally.
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UPLC–MS/MS performing pharmacokinetic and biodistribution studies of Rhein
Journal of separation science, 2012Co-Authors: Hui Sun, Quanwei Yin, Aihua Zhang, Xijun WangAbstract:Abstract Rhein, an important constituent of Radix et Rhizoma Rhei, has been used to alleviate liver and kidney damage. In this work, plasma pharmacokinetic and biodistribution characteristics of Rhein after oral administration was investigated using a rapid and sensitive ultra-performance liquid chromatography coupled to tandem high-definition mass spectrometry (UPLC-MS/MS) method. Mass spectrometry was performed on a Waters Micromass high-definition technology with an electrospray ionization source in positive ion mode. Biosamples were prepared using methanolic precipitation and the separation of Rhein was achieved on a UPLC HSS T3 column by linear gradient elution and the total run time was only 4.70 min. Data were analyzed and estimated by compartmental methods using Win-Nonlin Professional version 5.1. Mean pharmacokinetic parameters following single-dose administration of Rhein was consistent with a two-compartmental open model. It was found that Rhein was distributed and eliminated rapidly in rats and the biodistribution showed the higher levels were in liver, spleen, kidney, heart, lung and the lower level observed in the muscle, adrenal, and thyroid. It was not discovered in brain and showed that Rhein could not cross the blood-brain barrier. Our developed UPLC-MS/MS approach was capable of providing complete pharmacokinetic and biodistribution parameters for Rhein when administered orally.
Ruizhi Zhao - One of the best experts on this subject based on the ideXlab platform.
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liver targeting effect of vinegar baked radix bupleuri on Rhein in rats
Journal of Ethnopharmacology, 2010Co-Authors: Ruizhi Zhao, You-jun Chen, Dong Yuan, Ying ZhaoAbstract:Vinegar-baked Radix Bupleuri (VBRB) is usually used to focus other drugs effect on liver in traditional Chinese medicine (TCM). However, no sufficient scientific data are available to support this concept. In this paper, the liver targeting enhancing effect of VBRB on Rhein was investigated. 432 of rats were divided into two large groups according to the dose of Rhein, low dose group of Rhein (LDGR) and high dose group of Rhein (HDGR). In each group, the rats were further divided into four subgroups, Rhein control and Rhein co-administered with three different doses of VBRB peroral. Concentrations of Rhein and its metabolite in different tissues were determined by HPLC. Compared to the control group, VBRB significantly increased the distribution of both Rhein and its metabolite in liver and meanwhile decreased their distribution in other tissues, indicating a strong liver targeting enhancing effect. This liver targeting effect of VBRB depended on the dose of VBRB and Rhein. Low and high dose of VBRB had a more strong effect than medium dose in HDGR; high dose of Rhein was more sensitive than low dose of Rhein (P < 0.05). Rhein existed in two forms after peroral administration in vivo. It was found that the liver targeting effect of VBRB was more remarkable with the native form of Rhein compared to its derivative form. The results of this paper demonstrated that co-administration with VBRB is a simple and efficiencient method for liver targeting therapy, and the meridine guide theory of TCM was credible.
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Liver targeting effect of vinegar-baked Radix Bupleuri on Rhein in rats.
Journal of ethnopharmacology, 2010Co-Authors: Ruizhi Zhao, You-jun Chen, Dong Yuan, Shaojun Liu, Li Juan Liu, Ying ZhaoAbstract:Vinegar-baked Radix Bupleuri (VBRB) is usually used to focus other drugs effect on liver in traditional Chinese medicine (TCM). However, no sufficient scientific data are available to support this concept. In this paper, the liver targeting enhancing effect of VBRB on Rhein was investigated. 432 of rats were divided into two large groups according to the dose of Rhein, low dose group of Rhein (LDGR) and high dose group of Rhein (HDGR). In each group, the rats were further divided into four subgroups, Rhein control and Rhein co-administered with three different doses of VBRB peroral. Concentrations of Rhein and its metabolite in different tissues were determined by HPLC. Compared to the control group, VBRB significantly increased the distribution of both Rhein and its metabolite in liver and meanwhile decreased their distribution in other tissues, indicating a strong liver targeting enhancing effect. This liver targeting effect of VBRB depended on the dose of VBRB and Rhein. Low and high dose of VBRB had a more strong effect than medium dose in HDGR; high dose of Rhein was more sensitive than low dose of Rhein (P
