The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Don P. Wolf - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of novel Rhesus Monkey embryonic stem cell lines
Stem Cells, 2006Co-Authors: Shoukhrat Mitalipov, James Byrne, Lisa Clepper, Don P. Wolf, Hungchih Kuo, Lorraine F Meisner, Julie A Johnson, Renee ZeierAbstract:ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The Rhesus Monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from Rhesus Monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived Monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and Monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
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Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells
Biology of reproduction, 2006Co-Authors: James Byrne, Shoukhrat Mitalipov, Lisa Clepper, Don P. WolfAbstract:Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding survival, functionality, and tumor formation mean a prudent approach should be adopted towards advancing ESCs into human clinical trials. The Rhesus Monkey provides an ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety, and efficacy of ESC-based medical treatments. Transcriptional profiling of Rhesus Monkey ESCs provides a foundation for pre-clinical ESC research in this species. In the present study, we used microarray technology, immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) to characterize and transcriptionally profile Rhesus Monkey ESCs. We identified 367 stemness gene candidates that were highly (>85%) conserved across five different ESC lines. Rhesus Monkey ESC lines maintained a pluripotent undifferentiated state over a wide range of POU5F1 (also known as OCT4) expression levels, and comparisons between Rhesus Monkey, mouse, and human stemness genes revealed five mammalian stemness genes: CCNB1, GDF3, LEFTB, POU5F1, and NANOG. These five mammalian genes are strongly expressed in Rhesus Monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors.
Alan W. Thomas - One of the best experts on this subject based on the ideXlab platform.
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Detection of new Babesia microti-like parasites in a Rhesus Monkey (Macaca mulatta) with a suppressed Plasmodium cynomolgi infection.
The American journal of tropical medicine and hygiene, 2008Co-Authors: Annemarie Voorberg-v.d. Wel, Clemens H. M. Kocken, Anne-marie Zeeman, Alan W. ThomasAbstract:A new type of piroplasm, phylogenetically closest to Babesia microti-like parasites previously detected in Eurasian red squirrels (Sciurus vulgaris orientis), was identified in a Rhesus Monkey (Macaca mulatta) imported from China. After challenge with Plasmodium cynomolgi M strain blood-stage parasites, the Rhesus Monkey repeatedly showed markedly reduced levels of Plasmodium parasitemia when compared with animals not infected with this organism.
James Byrne - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of novel Rhesus Monkey embryonic stem cell lines
Stem Cells, 2006Co-Authors: Shoukhrat Mitalipov, James Byrne, Lisa Clepper, Don P. Wolf, Hungchih Kuo, Lorraine F Meisner, Julie A Johnson, Renee ZeierAbstract:ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The Rhesus Monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from Rhesus Monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived Monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and Monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
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Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells
Biology of reproduction, 2006Co-Authors: James Byrne, Shoukhrat Mitalipov, Lisa Clepper, Don P. WolfAbstract:Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding survival, functionality, and tumor formation mean a prudent approach should be adopted towards advancing ESCs into human clinical trials. The Rhesus Monkey provides an ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety, and efficacy of ESC-based medical treatments. Transcriptional profiling of Rhesus Monkey ESCs provides a foundation for pre-clinical ESC research in this species. In the present study, we used microarray technology, immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) to characterize and transcriptionally profile Rhesus Monkey ESCs. We identified 367 stemness gene candidates that were highly (>85%) conserved across five different ESC lines. Rhesus Monkey ESC lines maintained a pluripotent undifferentiated state over a wide range of POU5F1 (also known as OCT4) expression levels, and comparisons between Rhesus Monkey, mouse, and human stemness genes revealed five mammalian stemness genes: CCNB1, GDF3, LEFTB, POU5F1, and NANOG. These five mammalian genes are strongly expressed in Rhesus Monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors.
Shoukhrat Mitalipov - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of novel Rhesus Monkey embryonic stem cell lines
Stem Cells, 2006Co-Authors: Shoukhrat Mitalipov, James Byrne, Lisa Clepper, Don P. Wolf, Hungchih Kuo, Lorraine F Meisner, Julie A Johnson, Renee ZeierAbstract:ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The Rhesus Monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from Rhesus Monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived Monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and Monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
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Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells
Biology of reproduction, 2006Co-Authors: James Byrne, Shoukhrat Mitalipov, Lisa Clepper, Don P. WolfAbstract:Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding survival, functionality, and tumor formation mean a prudent approach should be adopted towards advancing ESCs into human clinical trials. The Rhesus Monkey provides an ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety, and efficacy of ESC-based medical treatments. Transcriptional profiling of Rhesus Monkey ESCs provides a foundation for pre-clinical ESC research in this species. In the present study, we used microarray technology, immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) to characterize and transcriptionally profile Rhesus Monkey ESCs. We identified 367 stemness gene candidates that were highly (>85%) conserved across five different ESC lines. Rhesus Monkey ESC lines maintained a pluripotent undifferentiated state over a wide range of POU5F1 (also known as OCT4) expression levels, and comparisons between Rhesus Monkey, mouse, and human stemness genes revealed five mammalian stemness genes: CCNB1, GDF3, LEFTB, POU5F1, and NANOG. These five mammalian genes are strongly expressed in Rhesus Monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors.
Lisa Clepper - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of novel Rhesus Monkey embryonic stem cell lines
Stem Cells, 2006Co-Authors: Shoukhrat Mitalipov, James Byrne, Lisa Clepper, Don P. Wolf, Hungchih Kuo, Lorraine F Meisner, Julie A Johnson, Renee ZeierAbstract:ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The Rhesus Monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from Rhesus Monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived Monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and Monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
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Transcriptional Profiling of Rhesus Monkey Embryonic Stem Cells
Biology of reproduction, 2006Co-Authors: James Byrne, Shoukhrat Mitalipov, Lisa Clepper, Don P. WolfAbstract:Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding survival, functionality, and tumor formation mean a prudent approach should be adopted towards advancing ESCs into human clinical trials. The Rhesus Monkey provides an ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety, and efficacy of ESC-based medical treatments. Transcriptional profiling of Rhesus Monkey ESCs provides a foundation for pre-clinical ESC research in this species. In the present study, we used microarray technology, immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) to characterize and transcriptionally profile Rhesus Monkey ESCs. We identified 367 stemness gene candidates that were highly (>85%) conserved across five different ESC lines. Rhesus Monkey ESC lines maintained a pluripotent undifferentiated state over a wide range of POU5F1 (also known as OCT4) expression levels, and comparisons between Rhesus Monkey, mouse, and human stemness genes revealed five mammalian stemness genes: CCNB1, GDF3, LEFTB, POU5F1, and NANOG. These five mammalian genes are strongly expressed in Rhesus Monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors.
