The Experts below are selected from a list of 2067 Experts worldwide ranked by ideXlab platform
Nicolas Granger - One of the best experts on this subject based on the ideXlab platform.
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Methods of olfactory ensheathing cell harvesting from the olfactory mucosa in dogs
2019Co-Authors: Daisuke Ito, Darren Carwardine, Jon Prager, Liang Fong Wong, Masato Kitagawa, Nick Jeffery, Nicolas GrangerAbstract:Olfactory ensheathing cells are thought to support regeneration and remyelination of damaged axons when transplanted into spinal cord injuries. Following transplantation, improved locomotion has been detected in many laboratory models and in dogs with naturally-occurring spinal cord injury; safety trials in humans have also been completed. For widespread clinical implementation, it will be necessary to derive large numbers of these cells from an accessible and, preferably, autologous, source making olfactory mucosa a good candidate. Here, we compared the yield of olfactory ensheathing cells from the olfactory mucosa using 3 different techniques: Rhinotomy, frontal sinus keyhole approach and rhinoscopy. From canine clinical cases with spinal cord injury, 27 biopsies were obtained by Rhinotomy, 7 by a keyhole approach and 1 with rhinoscopy. Biopsy via rhinoscopy was also tested in 13 cadavers and 7 living normal dogs. After 21 days of cell culture, the proportions and populations of p75-positive (presumed to be olfactory ensheathing) cells obtained by the keyhole approach and rhinoscopy were similar (~4.5 x 106 p75-positive cells; ~70% of the total cell population), but fewer were obtained by frontal sinus Rhinotomy. Cerebrospinal fluid rhinorrhea was observed in one dog and emphysema in 3 dogs following Rhinotomy. Blepharitis occurred in one dog after the keyhole approach. All three biopsy methods appear to be safe for harvesting a suitable number of olfactory ensheathing cells from the olfactory mucosa for transplantation within the spinal cord but each technique has specific advantages and drawbacks.
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Summary of Cell Proportion and Phenotypic Characteristics in Cultures Obtained by Rhinotomy (n = 27), Keyhole approach (n = 7) and Rhinoscopy (Cadaver n = 12, Living dog n = 7) at 21 Days In Vitro.
2019Co-Authors: Daisuke Ito, Darren Carwardine, Jon Prager, Liang Fong Wong, Masato Kitagawa, Nick Jeffery, Nicolas GrangerAbstract:Summary of Cell Proportion and Phenotypic Characteristics in Cultures Obtained by Rhinotomy (n = 27), Keyhole approach (n = 7) and Rhinoscopy (Cadaver n = 12, Living dog n = 7) at 21 Days In Vitro.
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Summary of Estimated Cell Population and Phenotypic Characteristics in Cultures Obtained by Rhinotomy (n = 27), Keyhole approach (n = 7) and Rhinoscopy (Cadaver n = 12, Living dog n = 7) at 21 Days In Vitro.
2019Co-Authors: Daisuke Ito, Darren Carwardine, Jon Prager, Liang Fong Wong, Masato Kitagawa, Nick Jeffery, Nicolas GrangerAbstract:Summary of Estimated Cell Population and Phenotypic Characteristics in Cultures Obtained by Rhinotomy (n = 27), Keyhole approach (n = 7) and Rhinoscopy (Cadaver n = 12, Living dog n = 7) at 21 Days In Vitro.
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Methods of olfactory ensheathing cell harvesting from the olfactory mucosa in dogs - Fig 6
2019Co-Authors: Daisuke Ito, Darren Carwardine, Jon Prager, Liang Fong Wong, Masato Kitagawa, Nick Jeffery, Nicolas GrangerAbstract:Comparison of proportion (left) and population (right) of p75 positive cells in between different harvesting methods. There were significant differences in p75-positive cell proportion and population in between cultures obtained by Rhinotomy and endoscopy (P
Anthony J. Reino - One of the best experts on this subject based on the ideXlab platform.
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Rhinotomy Approaches to the Paranasal Sinuses and Anterior Skull Base
Otolaryngology–Head and Neck Surgery, 1995Co-Authors: William Lawson, Anthony J. ReinoAbstract:Educational objectives: To understand and apply the three Rhinotomy approaches for extirpation of benign and malignant diseases of the sinonasal cavity, skull base, and anterior cranial fossa.
Han Yuefeng - One of the best experts on this subject based on the ideXlab platform.
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Preservation and reconstruction the lacrimal pas-sage in lateral Rhinotomy
Chinese Archives of Otolaryngology-head and Neck Surgery, 2005Co-Authors: Han YuefengAbstract:OBJECTIVE To investigate the preser-vation and reconstruction the lacrimal passage inlateral Rhinotomy. METHODS Bony lacrimal passageswere wholly opened in lateral Rhinotomy,so as toexpose lacrimal sac and lacrimonasal duct sufficiently.In cases with benign lesions,mucosal lacrimal passagesshould be preserved wholly. In cases with early malig-nant lesions,preserving lacrimal passage or not wasbased on the bony construction of lacrimal passage. Ifthe bony lacrimal passages were intact,mucosal lacri-mal passages must be preserved wholly. If bony lacrimalpassages were invaded suspiciously,the mucosal lacri-mal passages should be removed,remnant lacrimal sacwere used to dacryocystorhinostomy. RESULTS All31 cases cases underwent lateral Rhinotomy did nothave epiphora and lacrimal passage taint after operation.CONCLUSION Preservation and reconstruction thelacrimal passage and its physiological function inlateral Rhinotomy can enhance the quality of life of thepatients.
Matt S Ramer - One of the best experts on this subject based on the ideXlab platform.
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Spinally upregulated noggin suppresses axonal and dendritic plasticity following dorsal rhizotomy.
Experimental Neurology, 2006Co-Authors: David W. Hampton, John D Steeves, James W. Fawcett, Matt S RamerAbstract:Bone morphogenetic proteins (BMPs) and their antagonists, including noggin, are required for nervous system development, but their potential roles in the reactions of the adult central nervous system to injury are unknown. Here we have examined the expression of noggin and BMPs in the spinal cord following dorsal rhizotomy. Through the use of a function-blocking antibody, we have also investigated the role of endogenous noggin in the neuritic plasticity which follows rhizotomy. Dorsal rhizotomy resulted in the upregulation of BMPs 2/4, 7 and noggin in the superficial white matter and in the dorsal neuropil of the spinal cord. These co-localized with glial fibrillary acidic protein, indicating their expression by astrocytes. Because BMPs induce dendritic sprouting and synaptogenesis in some neuronal populations in vitro, we hypothesized that administration of a noggin function-blocking antibody (FbAb) in vivo would augment rhizotomy-induced sprouting in the spinal cord. Topical application of noggin-FbAb to the dorsal surface of the spinal cord following rhizotomy resulted in significant increases in the density of microtubule-associated protein 2 (MAP-2) and substance P (SP)-positive processes within the lateral spinal nucleus. In the deafferented dorsal horn, noggin-FbAb treatment induced significant increases in the density of SP, calcitonin gene-related peptide (CGRP)- and 5-hydroxytryptamine (5-HT)-positive axons. These results suggest a novel mechanism by which endogenous plasticity of spared axons is suppressed following dorsal rhizotomy, and which might be exploited to improve the outcome of spinal cord injury and other CNS trauma.
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rho kinase inhibition enhances axonal plasticity and attenuates cold hyperalgesia after dorsal rhizotomy
The Journal of Neuroscience, 2004Co-Authors: Leanne M Ramer, Jaimie F Borisoff, Matt S RamerAbstract:Dorsal rhizotomy results in primary deafferentation of the dorsal horn with concomitant sprouting of spared intraspinal monoaminergic axons. Because descending monoaminergic systems are thought to mitigate nociceptive transmission from the periphery and because dorsal rhizotomy can result in neuropathic pain, we sought to determine whether the rhizotomy-induced sprouting response could be further augmented. Because myelin-derived molecules mask endogenous plasticity of CNS axons and because myelin-inhibitory signaling occurs through the Rho-GTPase pathway, we inhibited Rho-pathway signaling after cervical dorsal rhizotomy in rats. An increase in the density of serotonergic- and tyrosine hydroxylase-positive fibers was seen in the dorsal horn 1 week after septuple rhizotomy, and axon density continued to increase for at least 1 month. One week after septuple rhizotomy, administration of intrathecal Y-27632, an antagonist of Rho-kinase (ROCK), increased the density of both fiber types over vehicle-treated controls. To examine behavioral effects of both cervical rhizotomy and ROCK inhibition, we examined responses to evoked pain: mechanical and thermal allodynia and cold hyperalgesia in the forepaw were examined after single, double, and quadruple rhizotomies of dorsal roots of the brachial plexus. The most notable behavioral outcome was the development of cold hyperalgesia in the affected forepaw after rhizotomies of the C7 and C8 dorsal roots. Application of Y-27632 both attenuated cold hyperalgesia and induced monoaminergic plasticity after C7/8 rhizotomy. Thus, inhibition of Rho-pathway signaling both promoted the sprouting of intact supraspinal monoaminergic fibers and alleviated pain after dorsal rhizotomy.
George W. Facer - One of the best experts on this subject based on the ideXlab platform.
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The clinical behavior of inverting papilloma of the nose and paranasal sinuses: report of 112 cases and review of the literature.
The Laryngoscope, 1990Co-Authors: P. Perry Phillips, Ray O. Gustafson, George W. FacerAbstract:Between 1944 and 1987, 112 patients with inverting papilloma of the nasal cavity were treated. The average duration of follow-up for this population was 6.2 years. The most common symptoms were nasal obstruction and history of previous surgery for nasal "polyps". Recurrence rates were lower when treatment consisted of lateral Rhinotomy with medial maxillectomy (14%) vs. transnasal operation with a sinus procedure (35%) or transnasal operation alone (58%). Recurrence rates between men and women were not significantly different when treatment methods were analyzed; however, a higher-than-expected association with tobacco usage was noted. Eight (7%) of the 112 patients had associated nasal carcinoma. Current treatment is lateral Rhinotomy with medial maxillectomy to prevent troublesome and potentially malignant recurrent disease.
