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Jinhyeuk Kwon - One of the best experts on this subject based on the ideXlab platform.
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occurrence of Rhizopus soft rot on rubus crataegifolius caused by Rhizopus Stolonifer in korea
Research in Plant Disease, 2008Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus Stolonifer on June, 2008 occurred on Rubus crataegifolius Bunge in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, black and eventually rotted. The symptoms occurred after infecting wounds at harvest time. The colony were white to light brown, formed much sporangiospores. The optimum temperature of the fungus on potato dextrose agar was . Sporangiophores were in width. Sporangia were globose or hemispheric, white at first and gradually to black, and in size. Columella were hemispheric, and in size. Sporangiospores were irregular round or oval, brownishblack and in size. On the basis of symptom, mycological characteristics and pathogenicity of the fungus, the causal fungus was identified as Rhizopus Stolonifer. This is the first report of Rhizopus soft rot by R. Stolonifer on R. crataegifolius in Korea.
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occurrence of Rhizopus soft rot on apple fruit caused by Rhizopus Stolonifer in korea
Research in Plant Disease, 2008Co-Authors: Jinhyeuk KwonAbstract:A Rhizopus soft rot caused by Rhizopus Stolonifer occurred sporadically on apple fruits (Malus pumila var. dulcissima Koidz.) at a wholesale market of agricultural products in Jinju, Korea in 2006 and 2007. Infected fruits were rapidly water-soaked, softened and rotted. The symptoms were initiated mainly from wounds or cracks occurred at harvest time. Sporangiophores were in length and in width. Sporangia were globose or hemispheric and in size. The color of sporangia was white and cottony at first and gradually turned to brownish black, bearing abundant spores when matured. Columella were hemispheric and in size. Sporangiospores were irregular round or oval, brownish-black streaked and in size. Optimum temperature for mycelial growth of the fungus on PDA was . On the basis of mycological characteristics and pathogenicity to apple the causal fungus was identified as Rhizopus Stolonifer (Ehrenb.) Vuill. This is the first report of Rhizopus soft rot by R. Stolonifer on Fuji apple in Korea.
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occurrence of Rhizopus soft rot on musa sapientum caused by Rhizopus Stolonifer in korea
The Korean Journal of Mycology, 2007Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus Stolonifer occurred on banana (Musa sapientum) in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, blackened and eventually rotted. They were infected from wounds at harvest time. The colony of the isolate from the infected fruit was white to light brown and formed many sporangiospores. The optimum growth temperature of the fungus on potato dextrose agar was . Sporangiophores were in width. Sporangia were globose or hemispheric, white at first and changed gradually to black, and in size. Columella were hemispheric, and in size. Sporangiospores were irregular round or oval, brownish-black and in size. On the basis of symptom, mycological characteristics and pathogenicity to banana, the causal fungus was identified as Rhizopus Stolonifer. This is the first report of Rhizopus soft rot by R. Stolonifer on banana in Korea.
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Rhizopus soft rot on pear pyrus serotina caused by Rhizopus Stolonifer in korea
Mycobiology, 2006Co-Authors: Jinhyeuk KwonAbstract:Rhizopus soft rot caused by Rhizopus Stolonifer occurred on pears (Pyrus serotina) in the Jinju City Agricultural Products Wholesale Market in Korea from 2004 to 2005. The infection usually started from wounds due to cracking at harvest time. The lesions started as water-soaked, rapidly softened, then gradually expanded. The mycelia grew vigorously on the surface of the fruits and formed stolons. Colonies on potato dextrose agar at 25℃ were white cottony to brownish black. Sporangia were globose, black and 90~120 µm in size. Sporangiophores were light brown and 480~2600 × 12~18 µm in size. Sporangiospores were globose to oval, brownish, streaked, and 8~14 × 6~10 µm in size. Columella were light brownish gray, hemispherical and 70~80 µm in size. On the basis of these symptoms, mycological characteristics and pathogenicity tests on host plants, the fungus was identified as Rhizopus Stolonifer (Ehrenb.) Vuill. This is the first report of Rhizopus soft rot on pear (P. serotina) caused by R. Stolonifer in Korea.
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Rhizopus soft rot on citrus fruit caused by Rhizopus Stolonifer in korea
The Korean Journal of Mycology, 2002Co-Authors: Jinhyeuk Kwon, Changseuk ParkAbstract:A fruits soft rot caused by Rhizopus Stolonifer found on citrus fruit in Jinjn City Agricultural Products Wholesale Market. The disease infection usually started from wounding. At first, the lesions started water soaked and rapidly softened and diseased lesion gradually expanded to other fruits in the same container. The mycelia grew vigorously on the surface of fruits and formed sporangia, sporangiophores and stolons. Sporangiophores were in size. Sporangia were globose or subglobose and in size. The color of sporangia was white at first and then turned black with many spores. Columella were globose or ellipsoid and in size. Sporangiospores were globose or ellipsoid, brownish-black streaked and in diameter. Zygospores were globose, black and in size. The optimum temperature for mycelial growth of the fungus on PDA was . The causal fungus was identified as Rhizopus Stolonifer. This is the first report of Rhizopus soft rot on citrus fruit caused by R. Stolonifer in Korea.
A N Hernandezlauzardo - One of the best experts on this subject based on the ideXlab platform.
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the mitochondrial respiratory chain of Rhizopus Stolonifer ehrenb fr vuill
Archives of Microbiology, 2013Co-Authors: Leobarda Roblesmartinez, Miguel Velazquezdel G Valle, A N Hernandezlauzardo, Maria Guadalupe Guerrasanchez, Oscar Floresherrera, Juan Pablo PardoAbstract:Rhizopus Stolonifer (Ehrenb.:Fr.) Vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes I, II, III, and IV) and the F1F0-ATP synthase (complex V). Using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. The composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organisms. Additionally, two alternative routes for the oxidation of cytosolic NADH were identified: the alternative NADH dehydrogenase and the glycerol-3-phosphate shuttles. Residual respiratory activity after inhibition of complex IV by cyanide was inhibited by low concentrations of n-octyl gallate, indicating the presence of an alternative oxidase. The K0.5 for the respiratory substrates NADH, succinate, and glycerol-3-phosphate in permeabilized cells was higher than in isolated mitochondria, suggesting that interactions of mitochondria with other cellular elements might be important for the function of this organelle.
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antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus Stolonifer ehrenb fr vuill
Carbohydrate Polymers, 2008Co-Authors: A N Hernandezlauzardo, Silvia Bautistabanos, M Velazquezdel G Valle, M G Mendezmontealvo, Mirna M Sanchezrivera, Luis A BelloperezAbstract:Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus Stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. Stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.
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estrategias de control de Rhizopus Stolonifer ehrenb ex fr lind agente causal de pudriciones postcosecha en productos agricolas
Revista Mexicana de Fitopatología, 2008Co-Authors: Miguel Velazquezdel G Valle, A N Hernandezlauzardo, Silvia Bautistabanos, Maria Guadalupe Guerrasanchez, Enriqueta AmoralazcanoAbstract:Resumen es: Rhizopus Stolonifer es un hongo fitopatogeno versatil que puede crecer y desarrollarse en una amplia gama de temperaturas y humedades relativas. Su rap...
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identification of Rhizopus Stolonifer ehrenb fr vuill causalagent of Rhizopus rot disease of fruits and vegetables
Revista Mexicana de Fitopatología, 2006Co-Authors: A N Hernandezlauzardo, Miguel Velazquezdel G Valle, Silvia Bautistabanos, Jose Luis TrejoespinoAbstract:Resumen en: Rhizopus Stolonifer commonly causes postharvest diseases on many fruits and vegetables. The objectives of this investigation were to evaluate the growt...
Silvia Bautistabanos - One of the best experts on this subject based on the ideXlab platform.
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Rhizopus Stolonifer soft rot
Postharvest Decay#R##N#Control Strategies, 2014Co-Authors: Silvia Bautistabanos, E Bosquezmolina, Laura Leticia BarreranechaAbstract:Abstract Rhizopus Stolonifer is considered the most important species in the genus Rhizopus . Disease symptoms that characterize R. Stolonifer infection are watery areas quickly covered by coarse, gray hairy mycelia forming a mass of black sporangia at their tips. Infection usually occurs during harvest and handling. Due to the wide array of hosts that R. Stolonifer can infect and its fast penetration and colonization, it has become an important target to control. Overall, R. Stolonifer is controlled using synthetic fungicides; among the various registered fungicides are thiabendazole, benomyl, imazalil, propiconazole and thiocarbamates. The fungicides fluodioxonyl, tebuconazole, fenhexamide and azoxystrobin have also achieved notable control of this fungus. Sanitizers like chlorine, sodium hypochlorite, calcium hypochlorite, ozone and peracetic acid have shown to have good control on Rhizopus . Of the various non-conventional methods under experimentation, antagonists have attracted considerable attention. Bacteria including Pantoe agglomerans , Enterobacter cloacae , Pseudomonas syringae , and Bacillus spp., as well as yeasts of the genera Pichia , Kloeckera , Candida , Cryptoccoccus , Metschnikowia and Acremonium , have exerted a significant control on this fungus. Aqueous plant extracts belonging to the botanical families Amaryllidaceae, Annonaceae, Arecaceae, Bromeliaceae, Caricaceae, Lamiaceae, Lauraceae, Leguminosae, Meliacea, Myrtaceae, Pinaceae, Sapotaceae, Solanaceae and Urticaceae, along with the essential oils of the genera Thymus , Cinnamom , Origanum and Citrus , have also produced excellent results under experimentation. UVC irradiation levels of up to 3.6 kJm 2 showed levels of control up to 50% on R. Stolonifer . Chitosan, a widely known chitin derivative, alone or in combination with other natural products, has been able to reduce significantly Rhizopus soft rot on various horticultural commodities at various concentrations. Volatiles, hypobaric atmospheres, salts, minerals, plant phenolics, hot water, wax and salicylic acid have resulted in good control methods with promising applications.
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antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus Stolonifer ehrenb fr vuill
Carbohydrate Polymers, 2008Co-Authors: A N Hernandezlauzardo, Silvia Bautistabanos, M Velazquezdel G Valle, M G Mendezmontealvo, Mirna M Sanchezrivera, Luis A BelloperezAbstract:Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopus Stolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. Stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.
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estrategias de control de Rhizopus Stolonifer ehrenb ex fr lind agente causal de pudriciones postcosecha en productos agricolas
Revista Mexicana de Fitopatología, 2008Co-Authors: Miguel Velazquezdel G Valle, A N Hernandezlauzardo, Silvia Bautistabanos, Maria Guadalupe Guerrasanchez, Enriqueta AmoralazcanoAbstract:Resumen es: Rhizopus Stolonifer es un hongo fitopatogeno versatil que puede crecer y desarrollarse en una amplia gama de temperaturas y humedades relativas. Su rap...
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identification of Rhizopus Stolonifer ehrenb fr vuill causalagent of Rhizopus rot disease of fruits and vegetables
Revista Mexicana de Fitopatología, 2006Co-Authors: A N Hernandezlauzardo, Miguel Velazquezdel G Valle, Silvia Bautistabanos, Jose Luis TrejoespinoAbstract:Resumen en: Rhizopus Stolonifer commonly causes postharvest diseases on many fruits and vegetables. The objectives of this investigation were to evaluate the growt...
Lixia Ding - One of the best experts on this subject based on the ideXlab platform.
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fermentation and purification of cellulase from a novel strain Rhizopus Stolonifer var reflexus tp 02
Biomass & Bioenergy, 2012Co-Authors: Bin Tang, Wenjing Tang, Qingqing Zhang, Lixia Ding, Fengqin ZhangAbstract:Abstract The morphology, cultural characteristics and internal transcribed spacer’s (ITS)-5.8S rDNA analysis of TP-02 were applied to identify a strain of filamentous fungus Rhizopus TP-02 with high activity of cellulase. The result of that showed that the novel strain could be identified as Zygomycotina , Phycomycetes , Mucorales , Mucoraceae , Rhizopus Stolonifer var. reflexus . The filter paper activity and CMC activity of the strain TP-02 could achieve 7.56 IU/mL and 28.5 IU/mL respectively after 96 h fermentation. During the tests of purification procedure, three types of Endo-β-1, 4-glucanases and two different molecular weight of β-1, 4-glucosidases were detected from the crud enzyme produced by the strain.
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cloning and expression of cellulase gene eg1 from Rhizopus Stolonifer var reflexus tp 02 in escherichia coli
Bioresource Technology, 2009Co-Authors: Bin Tang, Qingqing Zhang, Lixia DingAbstract:Abstract A novel gene, EG encoding enzymes involved in carboxymethyl cellulose (CMC) degradation was isolated, sequenced from the filamentous fungus Rhizopus Stolonifer var. reflexus TP-02, and expressed in Escherichia coli BL21. The results showed that the gene amplified from the cDNA of the strain could be classified as the family of endoglucanase. During the fermentation process, the maximum endoglucanase activity (i.e. 0.715 IU/ml) of the recombinant bacteria was obtained at 36 h. The SDS–PAGE analysis on purified samples showed that a band with apparent molecular weight of about 40 kDa was detected after staining with Coomassie brilliant blue.
Miguel Velazquezdel G Valle - One of the best experts on this subject based on the ideXlab platform.
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the mitochondrial respiratory chain of Rhizopus Stolonifer ehrenb fr vuill
Archives of Microbiology, 2013Co-Authors: Leobarda Roblesmartinez, Miguel Velazquezdel G Valle, A N Hernandezlauzardo, Maria Guadalupe Guerrasanchez, Oscar Floresherrera, Juan Pablo PardoAbstract:Rhizopus Stolonifer (Ehrenb.:Fr.) Vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes I, II, III, and IV) and the F1F0-ATP synthase (complex V). Using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. The composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organisms. Additionally, two alternative routes for the oxidation of cytosolic NADH were identified: the alternative NADH dehydrogenase and the glycerol-3-phosphate shuttles. Residual respiratory activity after inhibition of complex IV by cyanide was inhibited by low concentrations of n-octyl gallate, indicating the presence of an alternative oxidase. The K0.5 for the respiratory substrates NADH, succinate, and glycerol-3-phosphate in permeabilized cells was higher than in isolated mitochondria, suggesting that interactions of mitochondria with other cellular elements might be important for the function of this organelle.
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antifungal activity of chitosan and essential oils on Rhizopus Stolonifer ehrenb fr vuill causal agent of soft rot of tomato
Revista Colombiana de Biotecnología, 2011Co-Authors: Alejandra Maria Alvarado Hernandez, Laura Leticia Barrera Necha, Ana Niurka Hernandez Lauzardo, Miguel Velazquezdel G ValleAbstract:Rhizopus Stolonifer is the causal agent of soft rot, postharvest disease that causes important economic losses. Synthetic fungicides such as dichloran have been used to control this microorganism; however, it has been shown that fungicides represent a risk for the environment and human health. Actually, natural alternatives are looked for the control of postharvest rotting. In vitro and in situ experiments the antifungal effect of chitosan, essential oils of cinnamon (Cinnamomum zeylanicum), clove (Syzygium aromaticum), and thyme (Thymus vulgaris) and dichloran on Rhizopus Stolonifer were evaluated. The most effective treatments for in vitro inhibition of Rhizopus Stolonifer were obtained by quitosano to 10 mg mL, with the three essential oils proved to the concentration of 0.3 mg mL, the mixtures chitosan to 10 mg mL with the oils at 0.3 mg mL and dichloran at 1 mg mL. In situ experiments showed that the individual treatment with chitosan was the best to reduce the infection percentage of the tomato (Lycopersicon esculentum) fruits inoculated with Rhizopus Stolonifer and chitosan mixture with essential oils did not improve its antifungal activity. Chitosan and dichloran were the best treatments to reduce the weight loss of the fruits. Individual treatments with chitosan represent a natural alternative for the control of soft rot on tomato fruits.
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estrategias de control de Rhizopus Stolonifer ehrenb ex fr lind agente causal de pudriciones postcosecha en productos agricolas
Revista Mexicana de Fitopatología, 2008Co-Authors: Miguel Velazquezdel G Valle, A N Hernandezlauzardo, Silvia Bautistabanos, Maria Guadalupe Guerrasanchez, Enriqueta AmoralazcanoAbstract:Resumen es: Rhizopus Stolonifer es un hongo fitopatogeno versatil que puede crecer y desarrollarse en una amplia gama de temperaturas y humedades relativas. Su rap...
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identification of Rhizopus Stolonifer ehrenb fr vuill causalagent of Rhizopus rot disease of fruits and vegetables
Revista Mexicana de Fitopatología, 2006Co-Authors: A N Hernandezlauzardo, Miguel Velazquezdel G Valle, Silvia Bautistabanos, Jose Luis TrejoespinoAbstract:Resumen en: Rhizopus Stolonifer commonly causes postharvest diseases on many fruits and vegetables. The objectives of this investigation were to evaluate the growt...