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Ulrika Rova - One of the best experts on this subject based on the ideXlab platform.
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single cell oil producing yeasts lipomyces starkeyi and Rhodosporidium toruloides selection of extraction strategies and biodiesel property prediction
Energies, 2015Co-Authors: Nemailla Bonturi, Leonidas Matsakas, Robert Nilsson, Paul Christakopoulos, Everson Alves Miranda, Kris A Berglund, Ulrika RovaAbstract:Abstract: Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi . Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi ). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi ). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For
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Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides : Selection of Extraction Strategies and Biodiesel Property Prediction
Energies, 2015Co-Authors: Nemailla Bonturi, Leonidas Matsakas, Robert Nilsson, Paul Christakopoulos, Everson Alves Miranda, Kris A Berglund, Ulrika RovaAbstract:Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi . Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi ). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi ). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.
Nemailla Bonturi - One of the best experts on this subject based on the ideXlab platform.
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single cell oil producing yeasts lipomyces starkeyi and Rhodosporidium toruloides selection of extraction strategies and biodiesel property prediction
Energies, 2015Co-Authors: Nemailla Bonturi, Leonidas Matsakas, Robert Nilsson, Paul Christakopoulos, Everson Alves Miranda, Kris A Berglund, Ulrika RovaAbstract:Abstract: Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi . Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi ). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi ). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For
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Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides : Selection of Extraction Strategies and Biodiesel Property Prediction
Energies, 2015Co-Authors: Nemailla Bonturi, Leonidas Matsakas, Robert Nilsson, Paul Christakopoulos, Everson Alves Miranda, Kris A Berglund, Ulrika RovaAbstract:Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi . Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi ). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi ). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.
Ashwini Ashok Bedekar - One of the best experts on this subject based on the ideXlab platform.
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production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides ifo0880
Biotechnology for Biofuels, 2019Co-Authors: Sujit Sadashiv Jagtap, Ashwini Ashok BedekarAbstract:Background Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols.
Josa Paulo Sampaio - One of the best experts on this subject based on the ideXlab platform.
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polyphasic taxonomy of the basidiomycetous yeast genus rhodotorula rh glutinis sensu stricto and rh dairenensis comb nov
Fems Yeast Research, 2002Co-Authors: Mairio Gadanho, Josa Paulo SampaioAbstract:The phenotypic and genetic heterogeneity of the basidiomycetous yeast species Rhodotorula glutinis was investigated in a group of 109 isolates. A polyphasic taxonomic approach was followed which included PCR fingerprinting, determination of sexual compatibility, 26S and ITS rDNA sequence analysis, DNA–DNA reassociation experiments and reassessment of micromorphological and physiological attributes. The relationships with species of the teleomorphic genus Rhodosporidium were studied and isolates previously identified as Rh. glutinis were found to belong to Rhodosporidium babjevae, Rhodosporidium diobovatum and Rhodosporidium sphaerocarpum. Other isolates included in the study were found to belong to Rh. glutinis var. dairenensis, which is elevated to the species level, or to undescribed species. The concept of Rh. glutinis sensu stricto is proposed due to the close phenetic and phylogenetic proximity detected for Rh. glutinis, Rhodotorula graminis and R. babjevae.
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Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium: Rhodosporidium kratochvilovae and related anamorphic species.
International journal of systematic and evolutionary microbiology, 2001Co-Authors: Josa Paulo Sampaio, Mário Gadanho, S Santos, F L Duarte, C Pais, A Fonseca, J W FellAbstract:The phenotypic and genetic heterogeneity of the basidiomycetous yeast species Rhodosporidium kratochvilovae was investigated in a group of recent isolates and collection strains. A polyphasic taxonomic approach was followed which included micromorphological studies, nuclear staining, determination of sexual compatibility, physiological characterization, comparison of electrophoretic isoenzyme patterns, PCR fingerprinting, determination of mol% G+C, DNA-DNA reassociation experiments and 26S and ITS rDNA sequence analysis. The results allowed a more natural circumscription of the species, both from the genetic and phenotypic perspectives. The relationships with anamorphic species of the genus Rhodotorula were studied and isolates previously identified as Rhodotorula glutinis were found to belong to Rhodosporidium kratochvilovae. Other isolates included in the study were found to represent members of Rhodotorula glutinis var. dairenensis. Rhodosporidium kratochvilovae was found to include heterothallic strains, besides those already known to be self-sporulating. A total of 17 isolates, which were found to belong to this species, were heterothallic, self-sporulating and anamorphic strains. It is anticipated that integrated polyphasic studies of basidiomycetous yeasts will provide a more coherent classification system and the basis for accurate identification schemes, which in turn are essential for detailed ecological studies.
José Paulo Sampaio - One of the best experts on this subject based on the ideXlab platform.
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Rhodosporidium Banno (1967)
The Yeasts, 2011Co-Authors: José Paulo SampaioAbstract:Publisher Summary This chapter studies the genus Rhodosporidium. In the determination of asexual reproduction it is found that yeast cells are globose, ovoid, or elongate and budding is multilateral or polar. Ballistoconidia are not formed. Visible carotenoid pigments are formed and the cultures are pink to orange in color. In sexual reproduction it is seen that some species are heterothallic, whereas others are self-fertile. The hyphae have clamp connections. This chapter further examines physiology, biochemistry, and phylogenetic placement. The type species mentioned is Rhodosporidium toruloides. The species accepted are Rhodosporidium azoricum, Rhodosporidium babjevae, Rhodosporidium diobovatum, Rhodosporidium fluviale, Rhodosporidium kratochvilovae, Rhodosporidium lusitaniae, Rhodosporidium paludigenum, Rhodosporidium sphaerocarpum, and Rhodosporidium toruloides. The systematic discussion of the species includes growth on 5% malt extract agar, Dalmau plate culture on corn meal agar, sexual state, fermentation, gene sequence accession numbers, cell carbohydrates, origin of the strains studied, type strain, systematics, ecology, biotechnology, and agriculture and food.
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Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium: R. azoricum sp. nov.
Canadian journal of microbiology, 2001Co-Authors: Mário Gadanho, José Paulo Sampaio, Isabel Spencer-martinsAbstract:This report presents the description of a new heterothallic Rhodosporidium species, R. azoricum sp. nov. The new species is based on two strains previously identified as Rhodotorula glutinis, which were isolated from soil in Sao Miguel island, Azores, Portugal. Evidence that the two strains were conspecific and distinct from Rhodotorula glutinis was obtained in DNA fingerprinting experiments using the microsatellite-primed PCR approach (MSP-PCR) and the primers M13 and (GTG)5. In order to determine the phylogenetic position of the new species, the nucleotide sequence of the D1/D2 region of the 26S rDNA was analysed and Rhodosporidium azoricum was found to belong to a cluster including R. fluviale, R. lusitaniae, Sporidiobolus microsporus, and S. ruineniae. The life cycle of R. azoricum was investigated and comparisons integrating physiological, morphological, and molecular data were made with related species.Key words: Rhodosporidium azoricum sp. nov., microsatellite-primed PCR, Rhodotorula glutinis, basi...
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Rhodosporidium lusitaniae sp. nov., a Novel Homothallic Basidiomycetous Yeast Species from Portugal that Degrades Phenolic Compounds
Systematic and Applied Microbiology, 1992Co-Authors: Álvaro Fonseca, José Paulo SampaioAbstract:Summary Three strains of an undescribed basidiomycetous yeast, for which the name Rhodosporidium lusitaniae is proposed, were isolated from different substrates using either protocatechuic acid or vanillic acid as the sole source of carbon and energy in the isolation medium. They showed identical morphological, physiological and biochemical properties and shared the following characteristics: absence of ballistospores, production of carotenoid pigments, homothallic production of teliospores, formation of phragmo-metabasidia and failure to grow with inositol as sole source of carbon. This led us to classify them in the genus Rhodosporidium Banno. The new strains have some phenotypic similarity with the species Rhodosporidium dacryoidum . The molar G+C content of nDNA of the new strains is identical to the value determined for the type strain of Ro. dacryoidum but their nDNAs showed no hybridization indicating lack of conspecificity.
