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Jack W Fell - One of the best experts on this subject based on the ideXlab platform.
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Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions
Fems Yeast Research, 2002Co-Authors: Gloria Scorzetti, Alvaro Fonseca, Jack W Fell, Adele Statzell-tallmanAbstract:Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5′ end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
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systematics of basidiomycetous yeasts a comparison of large subunit d1 d2 and internal transcribed spacer rdna regions
Fems Yeast Research, 2002Co-Authors: Gloria Scorzetti, Alvaro Fonseca, Jack W Fell, Adele StatzelltallmanAbstract:Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5' end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
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biodiversity and systematics of basidiomycetous yeasts as determined by large subunit rdna d1 d2 domain sequence analysis
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jack W Fell, Alvaro Fonseca, Gloria Scorzetti, Teun Boekhout, Adele StatzelltallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
International journal of systematic and evolutionary microbiology, 2000Co-Authors: Jack W Fell, Gloria Scorzetti, Alvaro Fonseca, Teun Boekhout, Adele Statzell-tallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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87 – Sporidiobolus Nyland
The Yeasts, 1998Co-Authors: Adele Statzell-tallman, Jack W FellAbstract:Publisher Summary This chapter focuses on Sporidiobolus and its member species. The colony color of this genus on solid media includes shades of pink and red due to the presence of carotenoid pigments. Vegetative reproduction occurs through budding, and ballistoconidia develop on simple and branched sterigmata that arise from yeast and mycelial cells. Sexual mechanisms are homothallic and heterothallic. In the heterothallic system, a dikaryotic mycelium results fromthe conjugation of a compatible mating pair. Homothallism consists of the development of hyphae with teliospores from a single yeast cell in the absence ofmating. The yeast phase is diploid and reduction division takes place with the formation of a dikaryotic hypha with clamp connections. Germination of the teliospore results in a metabasidium with diploid basidiospores. The metabasidia are one-celled (holometabasidia) or septate with two or more cells (phragmometabasidia). The member species of this genus include Sporidiobolus johnsonii, Sporidiobolus pararoseus, Sporidiobolus ruineniae, and Sporidiobolus salmonicolor. The cells of Sporidiobolus johnsonii, afterundergoing growth in 5% malt extract for 3 days at 25° C, appear as ovoidal and elongate.
Teun Boekhout - One of the best experts on this subject based on the ideXlab platform.
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National Center for
2016Co-Authors: Lichenology Laboratory, Microorganisms Riken, Teun BoekhoutAbstract:and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffi
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Phylogenetic classification of yeasts and related taxa within Pucciniomycotina.
Studies in Mycology, 2015Co-Authors: Qi-ming Wang, Andrey Yurkov, Teun Boekhout, Markus Göker, H.t. Lumbsch, Steven D. Leavitt, Marizeth Groenewald, Bart Theelen, Xin-zhan Liu, Feng-yan BaiAbstract:Most small genera containing yeast species in the Pucciniomycotina (Basidiomycota, Fungi) are monophyletic, whereas larger genera including Bensingtonia, Rhodosporidium, Rhodotorula, Sporidiobolus and Sporobolomyces are polyphyletic. With the implementation of the “One Fungus = One Name” nomenclatural principle these polyphyletic genera were revised. Nine genera, namely Bannoa, Cystobasidiopsis, Colacogloea, Kondoa, Erythrobasidium, Rhodotorula, Sporobolomyces, Sakaguchia and Sterigmatomyces, were emended to include anamorphic and teleomorphic species based on the results obtained by a multi-gene phylogenetic analysis, phylogenetic network analyses, branch length-based methods, as well as morphological, physiological and biochemical comparisons. A new class Spiculogloeomycetes is proposed to accommodate the order Spiculogloeales. The new families Buckleyzymaceae with Buckleyzyma gen. nov., Chrysozymaceae with Chrysozyma gen. nov., Microsporomycetaceae with Microsporomyces gen. nov., Ruineniaceae with Ruinenia gen. nov., Symmetrosporaceae with Symmetrospora gen. nov., Colacogloeaceae and Sakaguchiaceae are proposed. The new genera Bannozyma, Buckleyzyma, Fellozyma, Hamamotoa, Hasegawazyma, Jianyunia, RhodoSporidiobolus, Oberwinklerozyma, Phenoliferia, Pseudobensingtonia, Pseudohyphozyma, Sampaiozyma, Slooffia, Spencerozyma, Trigonosporomyces, Udeniozyma, Vonarxula, Yamadamyces and Yunzhangia are proposed to accommodate species segregated from the genera Bensingtonia, Rhodosporidium, Rhodotorula, Sporidiobolus and Sporobolomyces. Ballistosporomyces is emended and reintroduced to include three Sporobolomyces species of the sasicola clade. A total of 111 new combinations are proposed in this study.
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Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov.
International journal of systematic and evolutionary microbiology, 2002Co-Authors: Feng-yan Bai, Masako Takashima, Jian-hua Zhao, Jian-hua Jia, Teun BoekhoutAbstract:More than 50 ballistoconidium-forming yeast strains, isolated from plant leaves collected in Yunnan, China, were identified as Sporobolomyces roseus Kluyver & van Niel by conventional methods. However, comparison of the internal transcribed spacer (ITS) region and 265 rDNA D1/D2 domain sequences indicated that these strains represented more than one species. Type or authentic strains of the synonyms of Sporobolomyces roseus and the closely related species Sporidiobolus pararoseus Fell & Tallman were employed in the rDNA sequence comparison. Sporobolomyces boleticola Ramírez, Sporobolomyces pollaccii Verona & Ciferri, Sporobolomyces roseus var. madurae Janke and Torulopsis somala Verona were confirmed to be conspecific with Sporobolomyces roseus. Another synonym of this species, Sporobolomyces salmoneus Derx, was located together with Sporobolomyces marcillae Santa Maria in a separate clade. Two synonyms of Sporidiobolus pararoseus, Sporobolomyces carnicolor Yamasaki & Fujii (nom. inval.) and Sporobolomyces japonicus Iizuka & Goto, were revealed to represent two distinct species. The name Sporobolomyces carnicolor is validated, with strain CBS 4215(T) as the type strain. A novel species represented by five of the selected Yunnan strains was confirmed, for which the name Sporobolomyces phaffii sp. nov. is proposed (type strain CH 2.052(T) = AS 2.2137(T) = JCM 11491(T) = CBS 9129(T)). This study also indicates that yeast species with similar ITS sequences may have quite different D1/D2 sequences.
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biodiversity and systematics of basidiomycetous yeasts as determined by large subunit rdna d1 d2 domain sequence analysis
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jack W Fell, Alvaro Fonseca, Gloria Scorzetti, Teun Boekhout, Adele StatzelltallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
International journal of systematic and evolutionary microbiology, 2000Co-Authors: Jack W Fell, Gloria Scorzetti, Alvaro Fonseca, Teun Boekhout, Adele Statzell-tallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
José Paulo Sampaio - One of the best experts on this subject based on the ideXlab platform.
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Sporidiobolus Nyland (1949)
The Yeasts, 2011Co-Authors: José Paulo SampaioAbstract:Publisher Summary This chapter studies the genus Sporidiobolus. In the determination of asexual reproduction it is found that reproduction is by budding and by formation of ballistoconidia. Yeast cells are ovoid, ellipsoidal, or elongate. Visible carotenoid pigments are formed and the cultures are pink to orange in color. In sexual reproduction it is seen that hyphae have clamp connections. Spherical teliospores are formed and germinate to produce transversely septate basidia with ovoid to bacilliform basidiospores, which are passively released and germinate by budding. In physiology and biochemistry it is seen that fermentation is absent. This chapter further examines phylogenetic placement. The type species mentioned is Sporidiobolus johnsonii. The species accepted are Sporidiobolus johnsonii, Sporidiobolus longiusculus, Sporidiobolus metaroseus, Sporidiobolus microsporus, Sporidiobolus pararoseus, Sporidiobolus ruineniae, and Sporidiobolus salmonicolor. The systematic discussion of the species includes synonyms, growth on 5% malt extract agar, Dalmau plate culture on corn meal agar, sexual state, CoQ, fermentation, Mol% G + C, gene sequence accession number, type strain, cell carbohydrates, origin of the strains studied, systematics, ecology, biotechnology, and agriculture and food.
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A deviation from the bipolar-tetrapolar mating paradigm in an early diverged basidiomycete.
PLoS genetics, 2010Co-Authors: José Paulo Sampaio, Marco A. Coelho, Paula GonçalvesAbstract:In fungi, sexual identity is determined by specialized genomic regions called MAT loci which are the equivalent to sex chromosomes in some animals and plants. Usually, only two sexes or mating types exist, which are determined by two alternate sets of genes (or alleles) at the MAT locus (bipolar system). However, in the phylum Basidiomycota, a unique tetrapolar system emerged in which four different mating types are generated per meiosis. This occurs because two functionally distinct molecular recognition systems, each encoded by one MAT region, constrain the selection of sexual partners. Heterozygosity at both MAT regions is a pre-requisite for mating in both bipolar and tetrapolar basidiomycetes. Tetrapolar mating behaviour results from the absence of genetic linkage between the two regions bringing forth up to thousands of mating types. The subphylum Pucciniomycotina, an early diverged lineage of basidiomycetes encompassing important plant pathogens such as the rusts and saprobes like Rhodosporidium and Sporidiobolus, has been so far poorly explored concerning the content and organization of MAT loci. Here we show that the red yeast Sporidiobolus salmonicolor has a mating system unlike any previously described because occasional disruptions of the genetic cohesion of the bipolar MAT locus originate new mating types. We confirmed that mating is normally bipolar and that heterozygosity at both MAT regions is required for mating. However, a laboratory cross showed that meiotic recombination may occur within the bipolar MAT locus, explaining tetrapolar features like increased allele number and evolution rates of some MAT genes. This pseudo-bipolar system deviates from the classical bipolar–tetrapolar paradigm and, to our knowledge, has never been observed before. We propose a model for MAT evolution in the Basidiomycota in which the pseudo-bipolar system may represent a hitherto unforeseen gradual form of transition from an ancestral tetrapolar system to bipolarity.
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Sporidiobolus johnsonii and Sporidiobolus salmonicolor revisited
Mycological Progress, 2008Co-Authors: Elisabete Valério, Mário Gadanho, José Paulo SampaioAbstract:The relationship between Sporidiobolus johnsonii and S. salmonicolor was investigated using rDNA sequence data. Two statistically well-supported clades were obtained. One clade included the type strain of S. johnsonii and the other included the type strain of S. salmonicolor. However, some mating strains of S. salmonicolor were found in the S. johnsonii group. These strains belonged to mating type A2 and were sexually compatible with mating type A1 strains from the S. salmonicolor group. DNA–DNA reassociation values were high within each clade and moderate between the two clades. In the re-investigation of teliospore germination, we observed that the basidia of S. salmonicolor were two-celled. In S. johnsonii , basidia were not formed and teliospore germination resulted in direct formation of yeast cells. We hypothesize that the S. johnsonii clade is becoming genetically isolated from the S. salmonicolor group and that a speciation process is presently going on. We suspect that the observed sexual compatibility between strains of the S. johnsonii and S. salmonicolor groups and the possible genetic flow between the two species has little biological relevance because distinct phenotypes have been fixed in the two taxa and intermediate (hybrid) sequences for LSU and ITS rDNAs have not been detected.
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Reappraisal of the Sporobolomyces roseus species complex and description of Sporidiobolus metaroseus sp. nov.
International journal of systematic and evolutionary microbiology, 2008Co-Authors: Elisabete Valério, Mário Gadanho, José Paulo SampaioAbstract:Here, we investigate a group of red to pinkish ballistoconidia-forming yeasts that were preliminarily identified as Sporobolomyces roseus or Sporidiobolus pararoseus. Detailed molecular and micromorphological studies revealed that the sexual strains and several conspecific anamorphic isolates belonged to a novel teleomorph that represents the sexual stage of Sporobolomyces roseus. Consequently, a new taxon in the genus Sporidiobolus is here described as Sporidiobolus metaroseus sp. nov. (type strain CBS 7683(T)). The main characteristics of Sporidiobolus metaroseus are presented and compared with those of the more closely related species. Our studies also led to the clarification of the life cycle of Sporidiobolus pararoseus. We confirm that the teliospores of this species germinate by forming short branches of hyphae, instead of basidia.
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Sporidiobolus longiusculus sp. nov. and Sporobolomyces patagonicus sp. nov., novel yeasts of the Sporidiobolales isolated from aquatic environments in Patagonia, Argentina.
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Diego Libkind, Mário Gadanho, María Van Broock, José Paulo SampaioAbstract:During a survey of carotenogenic yeasts carried out in north-western Patagonia (Argentina), several ballistoconidia-producing strains belonging to the order Sporidiobolales were isolated from aquatic environments. Five strains were found to represent two novel species, for which the names Sporidiobolus longiusculus and Sporobolomyces patagonicus are proposed, with CBS 9654T (=PYCC 5818T=CRUB 1044T) and CBS 9657T (=PYCC 5817T=CRUB 1038T) as the type strains, respectively. The elongated basidia, which are five to six times longer that those of the remaining species of the genus Sporidiobolus, are a particular micromorphological feature of Sporidiobolus longiusculus. On the basis of the sequences of the D1/D2 domains of the 26S rRNA gene, the species most closely related to Sporidiobolus longiusculus is Sporobolomyces bannaensis, whereas Sporobolomyces marcillae is the closest relative of Sporobolomyces patagonicus. Complete internal transcribed spacer sequence analysis confirmed the separate position of Sporidiobolus longiusculus, whereas for Sporobolomyces patagonicus no nucleotide differences were found with respect to Sporidiobolus pararoseus CBS 491T. Negative mating experiments between strains of Sporobolomyces patagonicus and strains of Sporidiobolus pararoseus together with the low DNA–DNA reassociation values for the type strains of the two species validated the proposal of Sporobolomyces patagonicus as a distinct species. Information on additional Patagonian Sporobolomyces isolates is also included in this report.
Adele Statzell-tallman - One of the best experts on this subject based on the ideXlab platform.
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Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions
Fems Yeast Research, 2002Co-Authors: Gloria Scorzetti, Alvaro Fonseca, Jack W Fell, Adele Statzell-tallmanAbstract:Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5′ end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
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Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
International journal of systematic and evolutionary microbiology, 2000Co-Authors: Jack W Fell, Gloria Scorzetti, Alvaro Fonseca, Teun Boekhout, Adele Statzell-tallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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87 – Sporidiobolus Nyland
The Yeasts, 1998Co-Authors: Adele Statzell-tallman, Jack W FellAbstract:Publisher Summary This chapter focuses on Sporidiobolus and its member species. The colony color of this genus on solid media includes shades of pink and red due to the presence of carotenoid pigments. Vegetative reproduction occurs through budding, and ballistoconidia develop on simple and branched sterigmata that arise from yeast and mycelial cells. Sexual mechanisms are homothallic and heterothallic. In the heterothallic system, a dikaryotic mycelium results fromthe conjugation of a compatible mating pair. Homothallism consists of the development of hyphae with teliospores from a single yeast cell in the absence ofmating. The yeast phase is diploid and reduction division takes place with the formation of a dikaryotic hypha with clamp connections. Germination of the teliospore results in a metabasidium with diploid basidiospores. The metabasidia are one-celled (holometabasidia) or septate with two or more cells (phragmometabasidia). The member species of this genus include Sporidiobolus johnsonii, Sporidiobolus pararoseus, Sporidiobolus ruineniae, and Sporidiobolus salmonicolor. The cells of Sporidiobolus johnsonii, afterundergoing growth in 5% malt extract for 3 days at 25° C, appear as ovoidal and elongate.
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Validation of the basidiomycetous yeast, Sporidiobolus microsporus sp. nov., based on phenotypic and molecular analyses.
Antonie van Leeuwenhoek, 1998Co-Authors: Jack W Fell, G.m. Blatt, Adele Statzell-tallmanAbstract:The validation of Sporidiobolus microsporus Higham, nom. nud. is based on phenotypic characterization and molecular sequence analysis of a partial region of the large sub-unit ribosomal DNA. The species is compared, based on phenotypic and molecular characteristics, with other species of Sporidiobolus and the closely related Rhodosporidium fluviale.
Gloria Scorzetti - One of the best experts on this subject based on the ideXlab platform.
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Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions
Fems Yeast Research, 2002Co-Authors: Gloria Scorzetti, Alvaro Fonseca, Jack W Fell, Adele Statzell-tallmanAbstract:Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5′ end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
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systematics of basidiomycetous yeasts a comparison of large subunit d1 d2 and internal transcribed spacer rdna regions
Fems Yeast Research, 2002Co-Authors: Gloria Scorzetti, Alvaro Fonseca, Jack W Fell, Adele StatzelltallmanAbstract:Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5' end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
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biodiversity and systematics of basidiomycetous yeasts as determined by large subunit rdna d1 d2 domain sequence analysis
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Jack W Fell, Alvaro Fonseca, Gloria Scorzetti, Teun Boekhout, Adele StatzelltallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
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Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
International journal of systematic and evolutionary microbiology, 2000Co-Authors: Jack W Fell, Gloria Scorzetti, Alvaro Fonseca, Teun Boekhout, Adele Statzell-tallmanAbstract:The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.
