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Lei Wang - One of the best experts on this subject based on the ideXlab platform.

  • transcriptomic study of the red palm weevil Rhynchophorus Ferrugineus embryogenesis
    Insect Science, 2015
    Co-Authors: Xiaowei Zhang, Lei Wang, Xiaoguang Yu, Khalid Alhudaib, Songnian Hu, Ibrahim S Almssallem
    Abstract:

    The red palm weevil (RPW), Rhynchophorus Ferrugineus (Coleoptera: Curculionidae), is an invasive, concealed and destructive tissue borer, and it becomes a lethal pest of the palm family of plants and has been reported to attack 20 palm species around the globe. Here we report a systematic transcriptomic study on embryogenesis of RPW, where we analyze the transcriptomes across five developmental stages of RPW embryogenesis, involving four embryonic stages (E1, E2, E3 and E4) and one larval stage (L1). Using the RNA-seq and next-generation platforms, we generated 80 to 91 million reads for each library and assemble 22 532 genes that are expressed at different embryonic stages. Among the total transcripts from the five embryonic development stages, we found that 30.45 % are differentially expressed, 10.10 % show stage-specificity and even a larger fraction, 62.88 %, exhibit constitutive expression in all the stages. We also analyzes the expression dynamics of several conserved signaling pathways (such as Hedgehog, JAK-STAT, Notch, TGF-β, Ras/MAPK and Wnt), as well as key developmental genes, including those related to apoptosis, axis formation, Hox complex, neurogenesis and segmentation. The datasets provide an essential resource for gene annotation and RPW functional genomics, including studies by using tools and concepts from multiple disciplines, such as development, physiology, biochemistry, molecular biology and genetics.

  • seasonally variable intestinal metagenomes of the red palm weevil Rhynchophorus Ferrugineus
    Environmental Microbiology, 2013
    Co-Authors: Xiaowei Zhang, Guangyu Zhang, Sun Zhang, Fusen Li, Lei Wang
    Abstract:

    The intestinal microbes residing in the red palm weevil (RPW, Rhynchophorus Ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (March, July and November), and then confirm the impact of high-temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify Klebsiella pneumoniae in November and Lactococcus lactis in July as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in November and July are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization.

Sandra Vacas - One of the best experts on this subject based on the ideXlab platform.

  • Development of an attract‐and‐infect system to control Rhynchophorus Ferrugineus with the entomopathogenic fungus Beauveria bassiana
    Pest Management Science, 2018
    Co-Authors: Óscar Dembilio, Josep A. Jaques, Sandra Vacas, Lola Ortega-garcía, Enrique Quesada-moraga, Pilar Moya, Vicente Navarro-llopis
    Abstract:

    This is the peer reviewed version of the following article: Dembilio, O. , Moya, P. , Vacas, S. , Ortega‐Garcia, L. , Quesada‐Moraga, E. , Jaques, J. A. and Navarro‐Llopis, V. (2018), Development of an attract‐and‐infect system to control Rhynchophorus Ferrugineus with the entomopathogenic fungus Beauveria bassiana. Pest. Manag. Sci, 74: 1861-1869., which has been published in final form at http://doi.org/10.1002/ps.4888. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving

  • Improvements in Rhynchophorus Ferrugineus (Coleoptera: Dryophthoridae) Trapping Systems.
    Journal of Economic Entomology, 2018
    Co-Authors: Vicente Navarro-llopis, Jaime Primo, Sandra Vacas
    Abstract:

    : Improved trap efficacy is crucial for implementing control methods for red palm weevil, Rhynchophorus Ferrugineus (Olivier; Coleoptera: Dryophthoridae), based on trapping systems, such as mass trapping, attract and infect or attract and sterilize techniques. Although new trap designs have been proposed and aggregation pheromone dispensers have been optimized, aspects such as the use of co-attractants (molasses) and trap placement are still not well defined and standardized. The efficacy of three concentrations of molasses and different formulations to reduce water evaporation in traps was studied in different field trials to improve trapping systems and to prolong trap servicing periods. In addition, the performance of installing groups of traps or single traps was also evaluated with the aim of improving the attracted/captured weevils ratio. Our results showed that captures increased when molasses were added at 15% to the water contained in the trap and that a thin layer of oil, created by adding 2-3% of paraffinic oil to water, was able to effectively reduce evaporation and prolong trap servicing periods. Moreover, 3.5-fold more weevils were captured when placing five traps instead of one at the same trapping point. Results obtained allow improved efficacy and may have an impact in the economic viability of trapping systems and, therefore, in integrated pest management programs.

  • identification of pheromone synergists for Rhynchophorus Ferrugineus trapping systems from phoenix canariensis palm volatiles
    Journal of Agricultural and Food Chemistry, 2014
    Co-Authors: Sandra Vacas, Jaime Primo, Maria Abadpaya, Vicente Navarrollopis
    Abstract:

    Trapping systems for the red palm weevil, Rhynchophorus Ferrugineus Olivier, rely on the use of natural plant odor sources to boost the attractiveness of the aggregation pheromone. The identification of the key odorants involved in attraction is essential in the development of a synthetic pheromone synergist to replace the nonstandardized use of plant material in traps. Canary Islands date palms (Phoenix canariensis) have become preferred hosts for R. Ferrugineus in Europe; thus, the volatile profile of different P. canariensis plant materials, including healthy and infested tissues, is investigated in the present work by means of solid phase microextraction (SPME-GC-MS), aimed to identify pheromone synergists. The electroantennography (EAG) response of the compounds identified was recorded, as well as the preliminary field response of several EAG-active compounds. The so-called “palm esters” (ethyl acetate, ethyl propionate, ethyl butyrate, and propyl butyrate) elicit the strongest EAG responses but perf...

Lu Peng - One of the best experts on this subject based on the ideXlab platform.

  • Red Palm Weevil Rhynchophorus Ferrugineus (Olivier)
    Biological Invasions and Its Management in China, 2017
    Co-Authors: Lu Peng
    Abstract:

    The red palm weevil (RPW), Rhynchophorus Ferrugineus (Coleoptera, Curculionidae) is the most destructive pest of palm trees worldwide. RPW can potentially spread to all palm-growing areas in China, mainly ranging across much of South, East, and Southwest areas and causing severe economic loss. Here, we introduce the current distribution of RPW in China, discuss the invasive mechanisms, including thermal adaptability, host adaptability, immune response, and population genetic differentiation, and summarize the frequently-used prevention and control strategies in China, with chemical controls accompanied by trapping are the dominated measures. We aim to summarize research progress in the past several decades in RPW and to provide sound prospects for improving the strategies and tactics currently employed in ecologically-based pest management in China. In addition, further studies will carry out from two aspects of prevention and control, mainly focus on building the early observing and monitoring system, better elucidating invasive mechanisms, as well as developing the new techniques for pest management, such as genetic and behavior regulation, biological pesticides screening, and natural enemies release.

  • Demographic comparison and population projection of Rhynchophorus Ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures
    Scientific Reports, 2016
    Co-Authors: Lu Peng, Yunxin Miao
    Abstract:

    Demographic comparison and population projection of Rhynchophorus Ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures

Xiaowei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • transcriptomic study of the red palm weevil Rhynchophorus Ferrugineus embryogenesis
    Insect Science, 2015
    Co-Authors: Xiaowei Zhang, Lei Wang, Xiaoguang Yu, Khalid Alhudaib, Songnian Hu, Ibrahim S Almssallem
    Abstract:

    The red palm weevil (RPW), Rhynchophorus Ferrugineus (Coleoptera: Curculionidae), is an invasive, concealed and destructive tissue borer, and it becomes a lethal pest of the palm family of plants and has been reported to attack 20 palm species around the globe. Here we report a systematic transcriptomic study on embryogenesis of RPW, where we analyze the transcriptomes across five developmental stages of RPW embryogenesis, involving four embryonic stages (E1, E2, E3 and E4) and one larval stage (L1). Using the RNA-seq and next-generation platforms, we generated 80 to 91 million reads for each library and assemble 22 532 genes that are expressed at different embryonic stages. Among the total transcripts from the five embryonic development stages, we found that 30.45 % are differentially expressed, 10.10 % show stage-specificity and even a larger fraction, 62.88 %, exhibit constitutive expression in all the stages. We also analyzes the expression dynamics of several conserved signaling pathways (such as Hedgehog, JAK-STAT, Notch, TGF-β, Ras/MAPK and Wnt), as well as key developmental genes, including those related to apoptosis, axis formation, Hox complex, neurogenesis and segmentation. The datasets provide an essential resource for gene annotation and RPW functional genomics, including studies by using tools and concepts from multiple disciplines, such as development, physiology, biochemistry, molecular biology and genetics.

  • seasonally variable intestinal metagenomes of the red palm weevil Rhynchophorus Ferrugineus
    Environmental Microbiology, 2013
    Co-Authors: Xiaowei Zhang, Guangyu Zhang, Sun Zhang, Fusen Li, Lei Wang
    Abstract:

    The intestinal microbes residing in the red palm weevil (RPW, Rhynchophorus Ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (March, July and November), and then confirm the impact of high-temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify Klebsiella pneumoniae in November and Lactococcus lactis in July as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in November and July are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization.

C.r.m. Nair - One of the best experts on this subject based on the ideXlab platform.

  • Electrophoretic protein pattern of male and female haemolymph of Rhynchophorus Ferrugineus Olivier
    International Journal of Farm Sciences, 2011
    Co-Authors: Annie Thomas, C.r.m. Nair
    Abstract:

    The protein pattern in the haemolymph of male and female Rhynchophorus Ferrugineus has been characterized. Native polyacrylamide gel electrophoresis (PAGE) analysis revealed eleven protein bands in adult males, ten in adult females and nine in the young female. Male haemolymph showed an additional protein band when compared with that of adult female haemolymph, whereas, in adult as well as young female a female specific protein band was present. The protein metabolism of an insect could be studied by observing the haemolymph protein pattern.

  • Variations in the haemocyte profile of Rhynchophorus Ferrugineus
    Indian journal of entomology, 2010
    Co-Authors: Annie Thomas, C.r.m. Nair
    Abstract:

    Haemocytes of Rhynchophorus Ferrugineus were described and figured as they appear in stained blood films. Six types of haemocytes were identified and they are prohaemocyte, plasmatocyte, granulocyte, adipohaemocyte, oenocytoid and spherulocyte. The haemogram profile noted in seven different stages revealed that there is a significant reduction in the count of granulocyte and plasmatocyte during pupation, which plays a key role in the defence against enemies.

  • Ultrastructure of the haemocytes of Rhynchophorus Ferrugineus Olivier
    Journal of entomological research, 2010
    Co-Authors: Annie Thomas, C.r.m. Nair
    Abstract:

    Electron microscopic studies showed the presence of six types of haemocytes in the haemolymph of Rhynchophorus Ferrugineus namely the prohaemocytes, plasmatocytes, granulocytes, adipohaemocytes, oenocytoids and spherulocytes. A comparison of cell size and nuclear size of haemocytes in the different stages of red palm weevil indicated that prohaemocytes are the smallest of all the haemocyte types, and adipohaemocytes are the biggest of all. All the cells in R. Ferrugineus were bigger in size during the early larval stages than the adult stages except plasmatocytes where reverse was true.