Root Exudates

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Shuyong Mu - One of the best experts on this subject based on the ideXlab platform.

  • lead complexation behaviour of Root Exudates of salt marsh plant salicornia europaea l
    Chemical Speciation and Bioavailability, 2012
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    AbstractRoot Exudates are considered to have an important role in mobility and bioavailability of heavy metals. High molecular weight (HMW) substances are the main components of Root Exudates, however, knowledge about their interactions with heavy metals is lacking. In the present study, Pb(II) complexation of the HMW fluorescent fractions in Root Exudates from Salicornia europaea L. was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectrum of Root Exudates. The fluorescence of both peaks was clearly quenched by Pb(II). The values of conditional stability constants, log Ka, for these two protein-like fluorescence peaks were 4.14 and 3.79. This indicates that the fluorescent substances are strong Pb(II) complexing organic ligands.

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Xi Chen, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

Jianying Yang - One of the best experts on this subject based on the ideXlab platform.

  • lead complexation behaviour of Root Exudates of salt marsh plant salicornia europaea l
    Chemical Speciation and Bioavailability, 2012
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    AbstractRoot Exudates are considered to have an important role in mobility and bioavailability of heavy metals. High molecular weight (HMW) substances are the main components of Root Exudates, however, knowledge about their interactions with heavy metals is lacking. In the present study, Pb(II) complexation of the HMW fluorescent fractions in Root Exudates from Salicornia europaea L. was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectrum of Root Exudates. The fluorescence of both peaks was clearly quenched by Pb(II). The values of conditional stability constants, log Ka, for these two protein-like fluorescence peaks were 4.14 and 3.79. This indicates that the fluorescent substances are strong Pb(II) complexing organic ligands.

  • lead complexation behaviour of Root Exudates of salt marsh plant salicornia europaea l
    Chemical Speciation and Bioavailability, 2012
    Co-Authors: Xiangliang Pan, Jianying Yang, Daoyong Zhang
    Abstract:

    Root Exudates are considered to have an important role in mobility and bioavailability of heavy metals. High molecular weight (HMW) substances are the main components of Root Exudates, however, kno...

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Xi Chen, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

Daoyong Zhang - One of the best experts on this subject based on the ideXlab platform.

  • lead complexation behaviour of Root Exudates of salt marsh plant salicornia europaea l
    Chemical Speciation and Bioavailability, 2012
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    AbstractRoot Exudates are considered to have an important role in mobility and bioavailability of heavy metals. High molecular weight (HMW) substances are the main components of Root Exudates, however, knowledge about their interactions with heavy metals is lacking. In the present study, Pb(II) complexation of the HMW fluorescent fractions in Root Exudates from Salicornia europaea L. was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectrum of Root Exudates. The fluorescence of both peaks was clearly quenched by Pb(II). The values of conditional stability constants, log Ka, for these two protein-like fluorescence peaks were 4.14 and 3.79. This indicates that the fluorescent substances are strong Pb(II) complexing organic ligands.

  • lead complexation behaviour of Root Exudates of salt marsh plant salicornia europaea l
    Chemical Speciation and Bioavailability, 2012
    Co-Authors: Xiangliang Pan, Jianying Yang, Daoyong Zhang
    Abstract:

    Root Exudates are considered to have an important role in mobility and bioavailability of heavy metals. High molecular weight (HMW) substances are the main components of Root Exudates, however, kno...

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Xi Chen, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

Xi Chen - One of the best experts on this subject based on the ideXlab platform.

  • cu ii complexation of high molecular weight hmw fluorescent substances in Root Exudates from a wetland halophyte salicornia europaea l
    Journal of Bioscience and Bioengineering, 2011
    Co-Authors: Jianying Yang, Daoyong Zhang, Xi Chen, Shuyong Mu
    Abstract:

    Abstract High molecular weight (HMW) fractions are important components in Root Exudates. However, there is little available information concerning complexation of Cu(II) to the HMW fractions in Root Exudates. In the present study, complexation of Root Exudates from Salicornia europaea L. with Cu(II) was investigated using excitation emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of Root Exudates. Fluorescence of both peaks was clearly quenched by Cu(II). The increase of conditional stability constant with increasing temperature indicates that the fluorescence quenching of the protein-like fluorescence by Cu(II) may be controlled by a dynamic process. The values of conditional stability constants (log K a ) were in the range of 4.32–4.69, which were close to those of complexation of fulvic acid with Cu(II). This shows that the HMW fluorescent substances in Root Exudates from S. europaea L. were strong organic ligands for Cu(II). Our study suggests that the HMW fluorescent substances may affect chemical forms, mobility, and thus the fate of copper in wetland.

Jorge M. Vivanco - One of the best experts on this subject based on the ideXlab platform.

  • Root Exudates drive soil microbe nutrient feedbacks in response to plant growth
    Plant Cell and Environment, 2021
    Co-Authors: Mengli Zhao, Jorge M. Vivanco, Jun Yuan, Jun Zhao, Lauren Hale, Tao Wen, Qiwei Huang, Jizhong Zhou
    Abstract:

    Although interactions between plants and microbes at the plant-soil interface are known to be important for plant nutrient acquisition, relatively little is known about how Root Exudates contribute to nutrient exchange over the course of plant development. In this study, Root Exudates from slow- and fast-growing stages of Arabidopsis thaliana plants were collected, chemically analysed and then applied to a sandy nutrient-depleted soil. We then tracked the impacts of these Exudates on soil bacterial communities, soil nutrients (ammonium, nitrate, available phosphorus and potassium) and plant growth. Both pools of Exudates shifted bacterial community structure. GeoChip analyses revealed increases in the functional gene potential of both exudate-treated soils, with similar responses observed for slow-growing and fast-growing plant exudate treatments. The fast-growing stage Root Exudates induced higher nutrient mineralization and enhanced plant growth as compared to treatments with slow-growing stage Exudates and the control. These results suggest that plants may adjust their exudation patterns over the course of their different growth phases to help tailor microbial recruitment to meet increased nutrient demands during periods demanding faster growth.

  • linking jasmonic acid signaling Root Exudates and rhizosphere microbiomes
    Molecular Plant-microbe Interactions, 2015
    Co-Authors: Lilia C Carvalhais, Dayakar V. Badri, Jorge M. Vivanco, Paul G Dennis, Brendan N Kidd, Peer M Schenk
    Abstract:

    Jasmonic acid (JA) is an essential hormone in plant development and defense responses in Arabidopsis thaliana. Exogenous treatment with JA has recently been shown to alter Root exudate profiles and the composition of Root-associated bacterial communities. However, it is currently unknown whether disruptions of the JA in the rhizosphere affect Root exudation profiles and the relative abundance of bacteria and archaea in the rhizosphere. In the present study, two Arabidopsis mutants that are disrupted in different branches of the jasmonate pathway, namely myc2 and med25, were cultivated in nutrient solution and soil to profile Root Exudates and bacterial and archaeal communities, respectively. Compared with the wild type, both mutants showed distinct exudation patterns, including lower amounts of asparagine, ornithine, and tryptophan, as well as distinct bacterial and archaeal community composition, as illustrated by an increased abundance of Streptomyces, Bacillus, and Lysinibacillus taxa in the med25 rhizosphere and an Enterobacteriaceae population in myc2. Alternatively, the Clostridiales population was less abundant in the rhizosphere of both mutants. Similarities between plant genotypes were highly correlated, as determined by operational taxonomic units in the rhizosphere and metabolites in Root Exudates. This strongly suggests that Root Exudates play a major role in modulating changes in microbial community composition upon plant defense responses.

  • organic acids from Root Exudates of banana help Root colonization of pgpr strain bacillus amyloliquefaciens njn 6
    Scientific Reports, 2015
    Co-Authors: Jun Yuan, Nan Zhang, Jorge M. Vivanco, Waseem Raza, Qiwei Huang, Qirong Shen
    Abstract:

    The successful colonization of plant growth promoting rhizobacteria (PGPR) in the rhizosphere is an initial and compulsory step in the protection of plants from soil-borne pathogens. Therefore, it is necessary to evaluate the role of Root Exudates in the colonization of PGPR. Banana Root Exudates were analyzed by high pressure liquid chromatography (HPLC) which revealed Exudates contained several organic acids (OAs) including oxalic, malic and fumaric acid. The chemotactic response and biofilm formation of Bacillus amyloliquefaciens NJN-6 were investigated in response to OA’s found in banana Root Exudates. Furthermore, the transcriptional levels of genes involved in biofilm formation, yqxM and epsD, were evaluated in response to OAs via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Results suggested that Root Exudates containing the OAs both induced the chemotaxis and biofilm formation in NJN-6. In fact, the strongest chemotactic and biofilm response was found when 50 μM of OAs were applied. More specifically, malic acid showed the greatest chemotactic response whereas fumaric acid significantly induced biofilm formation by a 20.7–27.3% increase and therefore biofilm formation genes expression. The results showed banana Root Exudates, in particular the OAs released, play a crucial role in attracting and initiating PGPR colonization on the host Roots.

  • Regulation and function of Root Exudates
    Plant Cell and Environment, 2008
    Co-Authors: Dayakar V. Badri, Jorge M. Vivanco
    Abstract:

    Root-secreted chemicals mediate multi-partite interactions in the rhizosphere, where plant Roots continually respond to and alter their immediate environment. Increasing evidence suggests that Root Exudates initiate and modulate dialogue between Roots and soil microbes. For example, Root Exudates serve as signals that initiate symbiosis with rhizobia and mycorrhizal fungi. In addition, Root Exudates maintain and support a highly specific diversity of microbes in the rhizosphere of a given particular plant species, thus suggesting a close evolutionary link. In this review, we focus mainly on compiling the information available on the regulation and mechanisms of Root exudation processes, and provide some ideas related to the evolutionary role of Root Exudates in shaping soil microbial communities.

  • Root Exudates Regulate Soil Fungal Community Composition and Diversity
    Applied and Environmental Microbiology, 2007
    Co-Authors: Corey D. Broeckling, Amanda K. Broz, Joy Bergelson, Daniel K. Manter, Jorge M. Vivanco
    Abstract:

    Plants are in constant contact with a community of soil biota that contains fungi ranging from pathogenic to symbiotic. A few studies have demonstrated a critical role of chemical communication in establishing highly specialized relationships, but the general role for Root Exudates in structuring the soil fungal community is poorly described. This study demonstrates that two model plant species (Arabidopsis thaliana and Medicago truncatula) are able to maintain resident soil fungal populations but unable to maintain nonresident soil fungal populations. This is mediated largely through Root Exudates: the effects of adding in vitro-generated Root Exudates to the soil fungal community were qualitatively and quantitatively similar to the results observed for plants grown in those same soils. This effect is observed for total fungal biomass, phylotype diversity, and overall community similarity to the starting community. Nonresident plants and Root Exudates influenced the fungal community by both positively and negatively impacting the relative abundance of individual phylotypes. A net increase in fungal biomass was observed when nonresident Root Exudates were added to resident plant treatments, suggesting that increases in specific carbon substrates and/or signaling compounds support an increased soil fungal population load. This study establishes Root Exudates as a mechanism through which a plant is able to regulate soil fungal community composition.